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Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4).

Abstract：

:Viruses protect their genomes by enclosing them into protein capsids that sometimes contain lipid bilayers that either reside above or below the protein layer. Controlled dissociation of virions provides important information on virion composition, interactions, and stoichiometry of virion components, as well as their possible role in virus life cycles. Dissociation of viruses can be achieved by using various chemicals, enzymatic treatments, and incubation conditions. Asymmetrical flow field-flow fractionation (AF4) is a gentle method where the separation is based on size. Here, we applied AF4 for controlled dissociation of enveloped bacteriophage φ6. Our results indicate that AF4 can be used to assay the efficiency of the dissociation process and to purify functional subviral particles.

journal_name

Viruses

journal_title

Viruses

authors

Eskelin K,Poranen MM

doi

10.3390/v10110579

subject

Has Abstract

pub_date

2018-10-23 00:00:00

issue

11

issn

1999-4915

pii

v10110579

journal_volume

10

pub_type

杂志文章

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