Abstract:
BACKGROUND:Duck plague virus (DPV) can induce apoptosis in duck embryo fibroblasts (DEFs) and in infected ducks, but the molecular mechanism of DPV-induced apoptosis remains unknown. METHODS:We first used qRT-PCR and a Caspase-Glo assay to determine whether the caspase protein family plays an important role in DPV-induced apoptosis. Then, we used an intracellular ROS detection kit and the mitochondrial probe JC-1 to respectively detect ROS levels and mitochondrial membrane potential (MMP). Finally, flow cytometry was used to detect apoptosis and cell cycle progression. RESULTS:In this study, the mRNA levels and enzymatic activities of caspase-3, caspase-7, caspase-8, and caspase-9 were significantly increased during DPV-induced apoptosis. The caspase inhibitors Z-DEVD-FMK, Z-LEHD-FMK, and Q-VD-OphA could inhibit DPV-induced apoptosis and promote viral replication. Subsequently, a significant decrease in MMP and an increase in the intracellular ROS levels were observed. Further study showed that pretreating infected cells with NAC (a ROS scavenger) decreased the intracellular ROS levels, increased the MMP, inhibited apoptosis, and promoted viral replication. Finally, we showed that DPV infection can cause cell cycle S-phase arrest. CONCLUSIONS:This study shows that DPV causes cell cycle S-phase arrest and leads to apoptosis through caspase activation and increased intracellular ROS levels. These findings may be useful for gaining an understanding of the pathogenesis of DPV and the apoptotic pathways induced by α-herpesviruses.
journal_name
Virusesjournal_title
Virusesauthors
Zhao C,Wang M,Cheng A,Yang Q,Wu Y,Jia R,Zhu D,Chen S,Liu M,Zhao X,Zhang S,Liu Y,Yu Y,Zhang L,Tian B,Rehman MU,Pan L,Chen Xdoi
10.3390/v11020196subject
Has Abstractpub_date
2019-02-24 00:00:00issue
2issn
1999-4915pii
v11020196journal_volume
11pub_type
杂志文章相关文献
Viruses-Basel文献大全abstract::Around 257 million people are living with hepatitis B virus (HBV) chronic infection and 71 million with hepatitis C virus (HCV) chronic infection. Both HBV and HCV infections can lead to liver complications such as cirrhosis and hepatocellular carcinoma (HCC). To take care of these chronically infected patients, one s...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v12121440
更新日期:2020-12-14 00:00:00
abstract::The RNA3 species of the beet necrotic yellow vein virus (BNYVV), a multipartite positive-stranded RNA phytovirus, contains the 'core' nucleotide sequence required for its systemic movement in Beta macrocarpa. Within this 'core' sequence resides a conserved "coremin" motif of 20 nucleotides that is absolutely essential...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10030137
更新日期:2018-03-19 00:00:00
abstract::The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system is widely explored for sequence-specific attack on HIV-1 proviral DNA. We recently identified dual-guide RNA (dual-gRNA) combinations that can block HIV-1 replication permanently in infected cell cultures and prevent viral escape. Altho...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11030255
更新日期:2019-03-13 00:00:00
abstract::The human cytomegalovirus (HCMV)-encoded protein kinase, pUL97, is considered a cyclin-dependent kinase (CDK) ortholog, due to shared structural and functional characteristics. The primary mechanism of CDK activation is binding to corresponding cyclins, including cyclin T1, which is the usual regulatory cofactor of CD...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v5123213
更新日期:2013-12-18 00:00:00
abstract::Gammaherpesviruses like Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) subvert the ubiquitin proteasome system for their own benefit in order to facilitate viral gene expression and replication. In particular, viral tegument proteins that share sequence homology to the formylglycineamide r...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v9100308
更新日期:2017-10-21 00:00:00
abstract::MicroRNAs (miRNAs) are non-coding but functional RNA molecules of 21-25 nucleotides in length. MiRNAs play significant regulatory roles in diverse plant biological processes. In order to decipher the relationship between nbe-miR1919c-5p and the accumulations of tobacco curly shoot virus (TbCSV) and its betasatellite (...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12040392
更新日期:2020-04-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) infection strongly modulates the host's immune response. The RNA silencing pathway is an intracellular innate response to viral infections. However, it is unknown whether PRRSV interacts with cellular RNA silencing to facilitate the viral infection. Here, we ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v7102893
更新日期:2015-10-23 00:00:00
abstract::Type I interferon (IFN-I) is the first line of mammalian host defense against viral infection. To counteract this, the flaviviruses, like other viruses, have encoded a variety of antagonists, and use a multi-layered molecular defense strategy to establish their infections. Among the most potent antagonists is non-stru...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v10120712
更新日期:2018-12-14 00:00:00
abstract::We describe herein, limitations on research at biosafety level 4 (BSL-4) containment laboratories, with regard to biosecurity regulations, safety considerations, research space limitations, and physical constraints in executing experimental procedures. These limitations can severely impact the number of collaborations...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v4123932
更新日期:2012-12-01 00:00:00
abstract::Peptide-displayed phage libraries are billion-clone collections of diverse chimeric bacteriophage particles, decorated by genetically fused peptides built from a random combination of natural amino acids. Studying the molecular evolution of peptide-displayed libraries in mammalian model systems, using in vivo phage di...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11110988
更新日期:2019-10-26 00:00:00
abstract::Phage lytic enzymes are enzymes produced by bacterial viruses, either as part of their virion to facilitate bacterial infection through local peptidoglycan degradation, or as soluble proteins to induce massive cell lysis at the end of the lytic replication cycle [...]. ...
journal_title:Viruses
pub_type: 社论
doi:10.3390/v11020113
更新日期:2019-01-29 00:00:00
abstract::Eukaryotic translation is a complex process composed of three main steps: initiation, elongation, and termination. During infections by RNA- and DNA-viruses, the eukaryotic translation machinery is used to assure optimal viral protein synthesis. Human immunodeficiency virus type I (HIV-1) uses several non-canonical pa...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v7010199
更新日期:2015-01-19 00:00:00
abstract::Lassa virus (LASV) and Mopeia virus (MOPV) are two closely related Old-World mammarenaviruses. LASV causes severe hemorrhagic fever with high mortality in humans, whereas no case of MOPV infection has been reported. Comparing MOPV and LASV is a powerful strategy to unravel pathogenic mechanisms that occur during the c...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11030293
更新日期:2019-03-23 00:00:00
abstract::The success of oral infection by viruses depends on their capacity to overcome the gut epithelial barrier of their host to crossing over apical, mucous extracellular matrices. As orally transmitted viruses, densoviruses, are also challenged by the complexity of the insect gut barriers, more specifically by the chitino...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11090870
更新日期:2019-09-17 00:00:00
abstract::Nasopharyngeal cancer (NPC) is associated with Epstein Barr virus (EBV) infection. However different viral strains have been implicated in NPC worldwide. This study aimed to detect and characterize EBV in patients diagnosed with NPC in Ghana. A total of 55 patients diagnosed with NPC by CT scan and endoscopy were age-...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12070766
更新日期:2020-07-16 00:00:00
abstract::The nervous system is the target for acute encephalitic viral infections, as well as a reservoir for persisting viruses. Intrathecal antibody (Ab) synthesis is well documented in humans afflicted by infections associated with neurological complications, as well as the demyelinating disease, multiple sclerosis. This re...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v5020732
更新日期:2013-02-15 00:00:00
abstract::Baboons are susceptible to natural Ebola virus (EBOV) infection and share 96% genetic homology with humans. Despite these characteristics, baboons have rarely been utilized as experimental models of human EBOV infection to evaluate the efficacy of prophylactics and therapeutics in the United States. This review will s...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v4102400
更新日期:2012-10-23 00:00:00
abstract::With ongoing colony losses driven in part by the Varroa mite and the associated exacerbation of the virus load, there is an urgent need to protect honey bees (Apis mellifera) from fatal levels of virus infection and from the non-target effects of insecticides used in agricultural settings. A continuously replicating c...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v12020236
更新日期:2020-02-20 00:00:00
abstract::Like all other viruses, a successful egress of functional particles from infected cells is a prerequisite for foamy virus (FV) spread within the host. The budding process of FVs involves steps, which are shared by other retroviruses, such as interaction of the capsid protein with components of cellular vacuolar protei...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v5041075
更新日期:2013-04-10 00:00:00
abstract::Virus diseases greatly affect oilseed rape (Brassica napus) production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene) with diffe...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v7082815
更新日期:2015-07-27 00:00:00
abstract::Plant positive strand RNA viruses are intracellular infectious agents that take advantage of cellular lipids and membranes to support replication and protect viral RNA from degradation by host antiviral responses. In this review, we discuss how Tomato bushy stunt virus (TBSV) co-opts lipid transfer proteins and modula...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v8030068
更新日期:2016-03-03 00:00:00
abstract::The extension of virology beyond its traditional medical, veterinary, or agricultural applications, now called environmental virology, has shown that viruses are both the most numerous and diverse biological entities on Earth. In particular, virus isolations from unicellular eukaryotic hosts (heterotrophic and photosy...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12101130
更新日期:2020-10-06 00:00:00
abstract::Avian influenza virus (AIV) is a highly diverse and widespread poultry pathogen. Itsevolution and adaptation may be affected by multiple host and ecological factors, which are stillpoorly understood. In the present study, a turkey-origin H9N2 AIV was used as a model toinvestigate the within-host diversity of the virus...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12030319
更新日期:2020-03-16 00:00:00
abstract::Lactococcus lactis is one of the most important bacteria in dairy fermentations, being used in the production of cheese and buttermilk. The processes are vulnerable to phage attacks, and undefined mixtures of lactococcal strains are often used to reduce the risk of bacteriophage caused fermentation failure. Other prev...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11050443
更新日期:2019-05-16 00:00:00
abstract::Influenza A viruses (IAV) have been a major public health threat worldwide, and options for antiviral therapy become increasingly limited with the emergence of drug-resisting virus strains. New and effective anti-IAV drugs, especially for highly pathogenic influenza, with different modes of action, are urgently needed...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12020225
更新日期:2020-02-18 00:00:00
abstract::Cytoskeleton defines the shape and structural organization of the cell. Its elements participate in cell motility, intracellular transport and chromosome movement during mitosis. Papillomaviruses (PV) are strictly epitheliotropic and induce self-limiting benign tumors of skin and mucosa, which may progress to malignan...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v3030260
更新日期:2011-03-01 00:00:00
abstract::Three dsRNAs, in sizes of approximately 2.5⁻5 kbp, were detected in the plant pathogenic fungus Nigrospora oryzae strain CS-7.5-4. Genomic analysis showed that the 5.0 kb dsRNA was a victorivirus named as Nigrospora oryzae victorivirus 2 (NoRV2). The genome of NoRV2 was 5166 bp in length containing two overlapping ope...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11010083
更新日期:2019-01-19 00:00:00
abstract::We developed an innovative strategy to induce a cytotoxic T cell (CTL) immune response against protein antigens of choice. It relies on the production of exosomes, i.e., nanovesicles spontaneously released by all cell types. We engineered the upload of huge amounts of protein antigens upon fusion with an anchoring pro...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v7031079
更新日期:2015-03-09 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) establishes latency in resting memory CD4+ T cells and cells of myeloid lineage. In contrast to the T cells, cells of myeloid lineage are resistant to the HIV-1 induced cytopathic effect. Cells of myeloid lineage including macrophages are present in anatomical sanctuaries ma...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v7041578
更新日期:2015-03-31 00:00:00
abstract::The Megalocytivirus genus includes three genotypes, red sea bream iridovirus (RSIV), infectious spleen and kidney necrosis virus (ISKNV), and turbot reddish body iridovirus (TRBIV), and has caused mass mortalities in various marine and freshwater fish species in East and Southeast Asia. Of the three genotypes, TRBIV-l...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12060681
更新日期:2020-06-24 00:00:00