Abstract：
:Avian influenza virus (AIV) is a highly diverse and widespread poultry pathogen. Itsevolution and adaptation may be affected by multiple host and ecological factors, which are stillpoorly understood. In the present study, a turkey-origin H9N2 AIV was used as a model toinvestigate the within-host diversity of the virus in turkeys, quail and ducks in conjunction with theclinical course, shedding and seroconversion. Ten birds were inoculated oculonasally with a doseof 106 EID50 of the virus and monitored for 14 days. Virus shedding, transmission andseroconversion were evaluated, and swabs collected at selected time-points were characterized indeep sequencing to assess virus diversity. In general, the virus showed low pathogenicity for theexamined bird species, but differences in shedding patterns, seroconversion and clinical outcomewere noted. The highest heterogeneity of the virus population as measured by the number of singlenucleotide polymorphisms and Shannon entropy was found in oropharyngeal swabs from quail,followed by turkeys and ducks. This suggests a strong bottleneck was imposed on the virus duringreplication in ducks, which can be explained by its poor adaptation and stronger selection pressurein waterfowl. The high within-host virus diversity in quail with high level of respiratory sheddingand asymptomatic course of infection may contribute to our understanding of the role of quail asan intermediate host for adaptation of AIV to other species of poultry. In contrast, low viruscomplexity was observed in cloacal swabs, mainly from turkeys, showing that the within-hostdiversity may vary between different replication sites. Consequences of these observations on thevirus evolution and adaptation require further investigation.
journal_name
Virusesjournal_title
Virusesauthors
Świętoń E,Tarasiuk K,Olszewska-Tomczyk M,Iwan E,Śmietanka Kdoi
10.3390/v12030319subject
Has Abstractpub_date
2020-03-16 00:00:00issue
3issn
1999-4915pii
v12030319journal_volume
12pub_type
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