Abstract:
:The virion proteins of Kaposi sarcoma-associated herpesvirus (KSHV) were initially characterized in 2005 in two separate studies that combined the detection of 24 viral proteins and a few cellular components via LC-MS/MS or MALDI-TOF. Despite considerable advances in the sensitivity and specificity of mass spectrometry instrumentation in recent years, leading to significantly higher yields in detections, the KSHV virion proteome has not been revisited. In this study, we have re-examined the protein composition of purified KSHV virions via ultra-high resolution Qq time-of-flight mass spectrometry (UHR-QqTOF). Our results confirm the detection of all previously reported virion proteins, in addition to 17 other viral proteins, some of which have been characterized as virion-associated using other methods, and 10 novel proteins identified as virion-associated for the first time in this study. These results add KSHV ORF9, ORF23, ORF35, ORF48, ORF58, ORF72/vCyclin, K3, K9/vIRF1, K10/vIRF4, and K10.5/vIRF3 to the list of KSHV proteins that can be incorporated into virions. The addition of these proteins to the KSHV virion proteome provides novel and important insight into early events in KSHV infection mediated by virion-associated proteins. Data are available via ProteomeXchange with identifier PXD022626.
journal_name
Virusesjournal_title
Virusesauthors
Nabiee R,Syed B,Ramirez Castano J,Lalani R,Totonchy JEdoi
10.3390/v12121382subject
Has Abstractpub_date
2020-12-02 00:00:00issue
12issn
1999-4915pii
v12121382journal_volume
12pub_type
杂志文章相关文献
Viruses-Basel文献大全abstract::During the last 25 years, swine vesicular disease (SVD) has occurred in Italy mostly sub-clinically. Therefore, regular testing of fecal samples from suspected holdings and high turnover premises was fundamental to identifying virus circulation and to achieve SVD eradication. In this study, we evaluated diagnostic per...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12111336
更新日期:2020-11-20 00:00:00
abstract::The Pneumoviridae family includes human metapneumovirus (HMPV) and human orthopneumovirus, which is also known as a respiratory syncytial virus (HRSV). These are large enveloped, negative single-strand RNA viruses. HMPV and HRSV are the human members, which commonly infect children. HMPV, which was discovered in 2001,...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12070781
更新日期:2020-07-20 00:00:00
abstract::Severe fever with thrombocytopenia syndrome (SFTS), a tick-borne infectious disease, is difficult to differentiate from other common febrile diseases. Clinically distinctive features and climate variates associated with tick growth can be useful predictors for SFTS. This retrospective study (2013-2019) demonstrated th...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v13010010
更新日期:2020-12-23 00:00:00
abstract::Several human monoclonal antibodies (hmAbs) and antibody fragments, including the best characterized in terms of structure-function b12 and Fab X5, exhibit relatively potent and broad HIV-1 neutralizing activity. However, the elicitation of b12 or b12-like antibodies in vivo by vaccine immunogens based on the HIV-1 en...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v1030802
更新日期:2009-12-01 00:00:00
abstract::Influenza virus infections can lead to viral pneumonia and acute respiratory distress syndrome in severe cases, causing significant morbidity and mortality and posing a great threat to human health. Because of the diversity of influenza virus strains and drug resistance to the current direct antiviral agents, there ha...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12050557
更新日期:2020-05-18 00:00:00
abstract::Pectobacterium atrosepticum is a phytopathogen of economic importance as it is the causative agent of potato blackleg and soft rot. Here we describe the Pectobacterium phage vB_PatP_CB5 (abbreviated as CB5), which specifically infects the bacterium. The bacteriophage is characterized in detail and TEM micrographs indi...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10080394
更新日期:2018-07-26 00:00:00
abstract::Despite worldwide efforts to understand the transmission dynamics of Zika virus (ZIKV), scanty evaluation has been made on the vector competence of Aedes aegypti fed directly on viremic human and non-human primates (NHPs). We blood-fed Ae. aegypti from two districts in Rio de Janeiro on six ZIKV infected pregnant rhes...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12121345
更新日期:2020-11-24 00:00:00
abstract::SARS-CoV-2 enters cells using its Spike protein, which is also the main target of neutralizing antibodies. Therefore, assays to measure how antibodies and sera affect Spike-mediated viral infection are important for studying immunity. Because SARS-CoV-2 is a biosafety-level-3 virus, one way to simplify such assays is ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12050513
更新日期:2020-05-06 00:00:00
abstract::Negative strand RNA viruses (NSVs) include many important human pathogens, such as influenza virus, Ebola virus, and rabies virus. One of the unique characteristics that NSVs share is the assembly of the nucleocapsid and its role in viral RNA synthesis. In NSVs, the single strand RNA genome is encapsidated in the line...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v12080835
更新日期:2020-07-30 00:00:00
abstract::In July 2018, a novel avian influenza virus (A/Mandarin duck/South Korea/KNU18-12/2018(H11N9)) was isolated from Mandarin ducks in South Korea. Phylogenetic and molecular analyses were conducted to characterize the genetic origins of the H11N9 strain. Phylogenetic analysis indicated that eight gene segments of strain ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12020203
更新日期:2020-02-12 00:00:00
abstract::Paramyxoviruses comprise a large number of diverse viruses which in part give rise to severe diseases in affected hosts. A new genotype of feline morbillivirus, tentatively named feline morbillivirus genotype 2 (FeMV-GT2), was isolated from urine of cats with urinary tract diseases. Whole genome sequencing showed abou...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11020146
更新日期:2019-02-09 00:00:00
abstract::Human respiratory syncytial virus (HRSV) is a major cause of pediatric infection and also causes disease in the elderly and those with underlying respiratory problems. There is no vaccine for HRSV and anti-viral therapeutics are not broadly applicable. To investigate the effect of HRSV biology in children, nasopharyng...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11100926
更新日期:2019-10-10 00:00:00
abstract::Interferon (IFN) regulatory factor 3 (IRF3) is the key transcription factor for the induction of IFN and antiviral genes. The absence of antiviral genes in IRF3 deficiency leads to susceptibility to a wide range of viral infections. Previously, we uncovered a function for nontranscriptional IRF3 (nt-IRF3), RLR (RIG-I-...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12040442
更新日期:2020-04-14 00:00:00
abstract::Most cases of hemorrhagic fever with renal syndrome (HFRS) in Europe are caused by the Puumala hantavirus (PUUV). Typical features of the disease are increased vascular permeability, acute kidney injury (AKI), and thrombocytopenia. YKL-40 is an inflammatory glycoprotein involved in various forms of acute and chronic i...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11090767
更新日期:2019-08-21 00:00:00
abstract::Viruses represent important test cases for data federation due to their genome size and the rapid increase in sequence data in publicly available databases. However, some consequences of previously decentralized (unfederated) data are lack of consensus or comparisons between feature annotations. Unifying or displaying...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12121424
更新日期:2020-12-10 00:00:00
abstract::Exosomes are membrane-enclosed vesicles actively released into the extracellular space, whose content reflect the physiological/pathological state of the cells they originate from. These vesicles participate in cell-to-cell communication and transfer of biologically active proteins, lipids, and RNAs. Their role in vir...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v7062770
更新日期:2015-06-19 00:00:00
abstract::Rotavirus (RV), a member of the Reoviridae family, causes infection in children and infants, with high morbidity and mortality. To be viable, the virus particle must package a set of eleven RNA segments. In order to understand the packaging mechanism, here, we co-synthesized sets of RNA segments in vitro in different ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v9070167
更新日期:2017-06-29 00:00:00
abstract::The Gammacoronavirus infectious bronchitis virus (IBV) causes a highly contagious and economically important respiratory disease in poultry. In the laboratory, most IBV strains are restricted to replication in ex vivo organ cultures or in ovo and do not replicate in cell culture, making the study of their basic virolo...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12101102
更新日期:2020-09-29 00:00:00
abstract::Porcine circovirus 2 (PCV2) is the etiological agent that causes porcine circovirus diseases and porcine circovirus-associated diseases (PCVD/PCVAD), which are present in every major swine-producing country in the world. PCV2 infections may downregulate the host immune system and enhance the infection and replication ...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v11020185
更新日期:2019-02-21 00:00:00
abstract::The family Totiviridae currently contains five genera Totivirus, Victorivirus, Leishmavirus, Trichomonasvirus, and Giardiavirus. Members in this family generally have a set of two-open reading frame (ORF) elements in their genome with the 5'-proximal ORF (ORF1) encoding a capsid protein (CP) and the 3'-proximal one (O...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11060577
更新日期:2019-06-25 00:00:00
abstract::Influenza A viruses (IAVs) harbor a segmented RNA genome that is organized into eight distinct viral ribonucleoprotein (vRNP) complexes. Although a segmented genome may be a major advantage to adapt to new host environments, it comes at the cost of a highly sophisticated genome packaging mechanism. Newly synthesized v...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v8060165
更新日期:2016-06-17 00:00:00
abstract::Noroviruses are the most common etiological agent of acute gastroenteritis worldwide. Despite their high infectivity, a subpopulation of individuals is resistant to infection and disease. This susceptibility is norovirus genotype-dependent and is largely mediated by the presence or absence of human histo-blood group a...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v11030226
更新日期:2019-03-06 00:00:00
abstract::Glycoprotein B (gB) plays an important role in alphaherpesvirus cellular entry and acts in concert with gD and the gH/gL complex. To evaluate whether functional differences exist between gB1 and gB4, the corresponding genes were exchanged between the two viruses. The gB4-containing-EHV-1 (EHV-1_gB4) recombinant virus ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v7020522
更新日期:2015-02-03 00:00:00
abstract::Endemic to West Africa and South America, mammalian arenaviruses can cross the species barrier from their natural rodent hosts to humans, resulting in illnesses ranging from mild flu-like syndromes to severe and fatal haemorrhagic zoonoses. The increased frequency of outbreaks and associated high fatality rates of the...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v12070784
更新日期:2020-07-21 00:00:00
abstract::Since the human immunodeficiency virus (HIV) was discovered as the etiological agent of acquired immunodeficiency syndrome (AIDS), it has encouraged much research into antiviral compounds. The reverse transcriptase (RT) of HIV has been a main target for antiviral drugs. However, all drugs developed so far inhibit the ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v2040900
更新日期:2010-04-01 00:00:00
abstract::The human gut contains an expanse of largely unstudied bacteriophages. Among the most common are crAss-like phages, which were predicted to infect Bacteriodetes hosts. CrAssphage, the first crAss-like phage to be discovered, contains a protein encoding a Bacteroides-associated carbohydrate-binding often N-terminal (BA...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v11121085
更新日期:2019-11-21 00:00:00
abstract::Usutu virus (USUV) is a neurotropic flavivirus closely related to West Nile virus (WNV). Its enzootic cycle mainly involves mosquitoes and birds. Human infection can occur with occasional, but sometimes severe, neurological complications. Since its emergence and spread in Europe over the last two decades, USUV has bee...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v12020164
更新日期:2020-01-31 00:00:00
abstract::Fruit trees, such as apricot trees, are constantly exposed to the attack of viruses. As they are propagated in a vegetative way, this risk is present not only in the field, where they remain for decades, but also during their propagation. Metagenomic diagnostic methods, based on next generation sequencing (NGS), offer...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v10060318
更新日期:2018-06-11 00:00:00
abstract::Foot-and-mouth disease virus (FMDV) causes a highly contagious disease with catastrophic economic impact for affected countries. BHK21 suspension cells are preferred for the industrial production of FMDV vaccine antigen, but not all virus strains can be successfully propagated in these cells. Serotype Asia-1 is often ...
journal_title:Viruses
pub_type: 杂志文章
doi:10.3390/v9080231
更新日期:2017-08-18 00:00:00
abstract::Coronaviruses are enveloped RNA viruses capable of causing respiratory, enteric, or systemic diseases in a variety of mammalian hosts that vary in clinical severity from subclinical to fatal. The host range and tissue tropism are largely determined by the coronaviral spike protein, which initiates cellular infection b...
journal_title:Viruses
pub_type: 杂志文章,评审
doi:10.3390/v12091023
更新日期:2020-09-13 00:00:00