Abstract:
PURPOSE:To determine whether chromosomal instability is induced in human diploid fibroblasts by ionizing radiation and to investigate the effects of radiation quality by comparing X-rays, neutrons and alpha-particles. MATERIALS AND METHODS:Cells from two human diploid fibroblast lines, HF12 and HF19, were irradiated and analysed cytogenetically at 3, 20 and 35 population doublings post-irradiation. RESULTS:Exposure of HF19 cells to neutrons and alpha-particles resulted in a consistently increased frequency of unstable aberrations, particularly chromatid-type aberrations, compared to control cultures. Aberration frequency after X-irradiation was not significantly greater than controls at 20 population doublings but was significantly increased after 35 population doublings, although not to the same level as that following neutron or alpha-irradiation. No chromosomal instability was demonstrated in the progeny of HF12 cells after X-, neutron or alpha-particle irradiation. CONCLUSIONS:The data are consistent with the progeny of irradiated HF19 cells expressing chromosomal instability. All three radiations are effective in inducing instability, but the expression of the phenotype is influenced by radiation quality. The absence of radiation-induced chromosomal instability in HF12 cells may reflect the influence of genetic factors.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Kadhim MA,Marsden SJ,Wright EGdoi
10.1080/095530098142518subject
Has Abstractpub_date
1998-02-01 00:00:00pages
143-8issue
2eissn
0955-3002issn
1362-3095journal_volume
73pub_type
杂志文章abstract::A review is presented to the program of education and training setup within the DoReMi Network of Excellence. DoReMi was funded by Euratom under the EU 7th Framework Programme to coordinate the EU research into risks from low-dose ionizing radiation. It was seen to be necessary to form a network of expert institutions...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2018.1454616
更新日期:2019-01-01 00:00:00
abstract::Studies of protein phosphorylation in the second cell cycle of mouse embryos were assessed by [32P]-incorporation and one- and two-dimensional electrophoresis. Unirradiated and X-irradiated embryos were compared. X-irradiation in the G2 phase led to a block in this cell cycle phase. The phosphorylation of proteins wit...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114552351
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journal_title:International journal of radiation biology
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abstract:PURPOSE:The actinides are chemical poisons and radiological hazards. One challenge to better appraise their toxicity and develop countermeasures in case of exposure of living organisms is to better assess pathways of contamination. Because of the high chemical affinity of those actinide elements for phosphate groups an...
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pub_type: 杂志文章
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更新日期:2014-11-01 00:00:00
abstract:PURPOSE:The assessment of biological effects caused by radiation exposure has been currently carried out with the linear-quadratic (LQ) model as an extension of the linear non-threshold (LNT) model. In this study, we suggest a new mathematical model named as SeaSaw (SS) model, which describes proliferation and cell dea...
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:
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abstract:PURPOSE:Ionizing radiation (IR) produced a dose-dependent increase in apoptosis in U937/pCEP4 cells which was attenuated by the stable over expression of Bcl-2 (U937/Bcl-2). A dose of 2 Gy IR was selected for further analyses to determine if subsequent exposure to 10nM bryostatin- would overcome the resistance to IR-in...
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更新日期:2006-08-01 00:00:00
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journal_title:International journal of radiation biology
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更新日期:1997-07-01 00:00:00
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pub_type: 历史文章
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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更新日期:1990-09-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214552121
更新日期:1992-09-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
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更新日期:1990-04-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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更新日期:1996-01-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214551241
更新日期:1992-04-01 00:00:00