Abstract：
:For the interpretation of the concentration of morphine in blood samples of heroin consumers information about the concentration of the analgesic active morphine metabolite morphine-6-glucuronide is very important. Thus a simple but specific clean-up procedure based on immuno-affinity chromatography is presented for the extraction of morphine, morphine-3-glucuronide and morphine-6-glucuronide from whole blood in cases of fatal heroin overdose. The preparation of the immunoabsorber by immobilization of antibodies against morphine-3-BSA and morphine-6-KLH with carbonyldiimidazole-activated trisacrylgel is described. The separation of the extracts is achieved by HPLC using native fluorescence detection. The limits of detection for this method are 10ng for morphine and morphine glucuronides/g blood. The results for the concentration of morphine and morphine glucuronides in blood from seven cases of heroin overdose are presented. By calculating the quotients for the concentrations of morphine-6-glucuronide/morphine the time elapsed since the last intake of heroin is estimated.
journal_name
Int J Legal Medjournal_title
International journal of legal medicineauthors
Beike J,Köhler H,Blaschke Gdoi
10.1007/s004140050073subject
Has Abstractpub_date
1997-01-01 00:00:00pages
226-9issue
4eissn
0937-9827issn
1437-1596journal_volume
110pub_type
杂志文章abstract：:We present a case of fatal intoxication by the application of a transdermal fentanyl patch upon a superficial bleeding abrasion of a 2-year-old girl. The grandmother discovered the body of the child in bed at approximately 7 a.m. External examination revealed a properly developed, nourished, and hydrated child, with s...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1209-z
更新日期：2015-11-01 00:00:00
abstract：:STR-based individualisation of biological deposits on bullets after perforation of tissue, can identify the person injured or killed by a particular bullet and comparison with the firearms used can identify the weapon and thus possibly the person who did the shooting. In this study, the effect of subsequent impacts on...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140000203
更新日期：2001-01-01 00:00:00
abstract：:In the present study 161 Japanese father/son haplotype transfers in 147 pedigrees were analyzed at 14 Y-STRs with two multiplex PCR-based typing systems. Five isolated single repeat mutations were identified at the DYS389I, DYS439, Y-GATA-H4, DYS389II and DYS391 loci, and a pedigree showing triple alleles at the DYS38...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-003-0422-3
更新日期：2004-06-01 00:00:00
abstract：:In many forensic cases, the existence of a traumatic brain injury (TBI) is an essential factor, and the determination of the survival time is nearly as important as the determination of whether or not a trauma exists. Since it is known that glucose uptake increases in injured brain cells in order to perpetuate the neu...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-018-1893-6
更新日期：2019-07-01 00:00:00
abstract：:Current analysis of forensic DNA stains relies on the probabilistic interpretation of bulk-processed samples that represent mixed profiles consisting of an unknown number of potentially partial representations of each contributor. Single-cell methods, in contrast, offer a solution to the forensic DNA mixture problem b...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-021-02503-4
更新日期：2021-01-23 00:00:00
abstract：:The use of phenethylamines in the dance scene is now well established. Apart from amphetamine, the commonest phenethylamine encountered in clinical and forensic settings is 3,4-methylenedioxymethamphetamine (MDMA) commonly known as ecstasy. Other phenethylamines, which have similar effects are encountered, such as 3,4...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050291
更新日期：2000-01-01 00:00:00
abstract：:Allele frequencies and forensic parameters for 17 autosomal short tandem repeat (STR) loci were investigated from 1542 individuals of Va ethnic minority in Yunnan Province in the southwest of China. Also, genetic distances between Va and 13 published populations were compared, and a neighbor-joining (NJ) phylogenetic ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1620-8
更新日期：2017-09-01 00:00:00
abstract：:Severe cold stress has been shown to cause changes in the expression and secretion of thrombomodulin (TM), an endothelial protein regulating haemostasis and inflammation. To further evaluate TM as a cold stress indicator, relative TM mRNA and TM protein levels in the myocardium and the concentrations of TM in serum an...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-014-1138-2
更新日期：2015-03-01 00:00:00
abstract：:Warping has been used to determine the pre-burning condition of human skeletal remains. In the literature, this modification has been associated more often with the burning of fleshed and green bones, but it also arises during the burning of dry bones. The objective of this paper was to assess if bone collagen content...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1400-x
更新日期：2016-11-01 00:00:00
abstract：:The tetrameric STRs, HUMTH01, HUMVWA31A and HUMFES/FPS, were studied in a population from the Basque Country (northern Spain) for their frequency distribution and applicability to identity and paternity testing. All systems conformed to Hardy-Weinberg equilibrium; pairwise comparisons demonstrated the allelic independ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01245481
更新日期：1995-01-01 00:00:00
abstract：:Allele frequencies at the short tandem repeat (STR) loci HumACTBP2 and HumVWA were determined in 118 unrelated individuals from Northern Italy (Milan area). For locus HumACTBP2 (SE33) a total of 39 alleles was observed. Furthermore, two interalleles (N18m+N19m) and one allele (> N35) were found which were not observed...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050090
更新日期：1997-01-01 00:00:00
abstract：:To study the population data of Y-chromosome STRs (Y-STRs) of Han population resided in Hunan province, we analyzed haplotypes of 26 Y-STRs (DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, DYS38...
journal_title：International journal of legal medicine
pub_type： 杂志文章,评审
doi：10.1007/s00414-016-1411-7
更新日期：2017-01-01 00:00:00
abstract：:DNA typing was carried out on epithelial cells which were transferred from the hands of the suspect onto the neck of the victim. In an experimental study 16 suspect-victim combinations were investigated for estimating the typing success. Alternatively to an attack against the neck, the upper arm was used for "strangul...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050063
更新日期：1997-01-01 00:00:00
abstract：:In the forensic anthropology practice, bone diseases are rarely considered for personal identification. In this paper, we present a forensic skeletonized case with tuberculous bone lesions, for which bone pathology may provide an indicator for positive personal identification. Antemortem hospital records were availabl...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02348-3
更新日期：2020-09-01 00:00:00
abstract：:Growth arrest-specific 6 (Gas6) is widely expressed in leukocytes, platelets, endothelial cells, and monocytes. It regulates various processes including granulocyte adhesion to the endothelium, cell migration, thrombus stabilization, and cytokine release. In humans, increased plasma Gas6 levels have been described in ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1230-2
更新日期：2015-09-01 00:00:00
abstract：:Diagnosis of ischaemia-related sudden cardiac death in the absence of microscopic and macroscopic ischaemic lesions remains a challenge for medical examiners. Medical imaging techniques increasingly provide support in post-mortem examinations by detecting and documenting internal findings prior to autopsy. Previous st...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-019-02211-0
更新日期：2020-03-01 00:00:00
abstract：:In kinship analysis, large data sets with estimated haplotype frequencies for marker clusters are very important for the likelihood calculation. Practical use of the X-STRs demonstrated that in some complex kinship cases, the marker set of the Investigator Argus X-12 kit can be insufficient. This study aimed to extend...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-020-02306-z
更新日期：2020-11-01 00:00:00
abstract：:Limited genetic marker information can be obtained from saliva by typing by conventional serological means. Thus, the application of PCR-based DNA typing methods was investigated as a potential approach for typing genetic markers in saliva. DNA was isolated from 200 cigarettes smoked by 10 different individuals (20 ci...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF01369812
更新日期：1991-01-01 00:00:00
abstract：:In order to establish a reliable age indicator in the period when all other epiphyseal age indicators have already been inactivated, medial clavicle as the bone with the longest period of growth became the object of various investigations. However, the lack of population-specific method often made it unreliable in som...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0791-6
更新日期：2013-05-01 00:00:00
abstract：:Myocardial infarctions go along with biomechanical stress, i.e. stretching of muscle fibres, and the expression of certain marker molecules. We tested if two of those markers, endothelin-1 (ET-1) and growth differentiation factor 15 (GDF-15), can be used as immunohistochemical markers for myocardial ischaemia/infarcti...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1726-z
更新日期：2018-03-01 00:00:00
abstract：:This paper describes the general design and main results of the Italian proficiency testing program for the analysis of psychoactive substances in urine, a long-term initiative created in 1995 on an educational basis and characterized by an innovative internet-based service for data exchange between laboratories and t...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050279
更新日期：1999-01-01 00:00:00
abstract：:Chumbinho is the popular name given to carbamate aldicarb (Temik), an insecticide commonly used in agriculture and highly toxic (LD50 = 0.9 mg/kg oral in rats) that has been sold clandestinely in several regions of Brazil. Chumbinho is sold illegally as raticide and is available in a formulation of small black granule...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-016-1336-1
更新日期：2016-07-01 00:00:00
abstract：:Blank cartridge guns are generally regarded as being harmless and are not considered to be firearms in most countries. A comparison of the legal situations in Germany and Austria concerning weapons is given. There have been several reports of serious injuries and even fatalities due to these weapons. Ballistic experim...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140050156
更新日期：1998-01-01 00:00:00
abstract：:One of the most fundamental issues in forensic anthropology is the determination of sex and population affinity based on various skeletal elements. Therefore, we compared the sexual dimorphism of the upper facial skeleton from a recent Czech population (twenty-first century) with that of a population from Early Modern...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-017-1625-3
更新日期：2018-01-01 00:00:00
abstract：:The performance of two detection techniques for body fluids, the Spectra-Physics(R) Revealtrade mark portable forensic laser system and the mercury-arc lamp Lumatec Superlite 400, was evaluated with various biological stains on different substrates. Serial dilutions of neat, 1/10, 1/100 and 1/1,000 using fluid semen, ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-007-0214-2
更新日期：2008-05-01 00:00:00
abstract：:The smoking cessation agent varenicline acts as a partial agonist on α(4)β(2) nicotinic acetylcholine receptors. Although debated, several reports have linked varenicline therapy to an increased risk of suicidal thoughts and/or suicide. In addition, several non-fatal overdose cases have been reported. In this report, ...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-012-0695-5
更新日期：2013-01-01 00:00:00
abstract：:Plant exposures are among the most frequently reported cases to poison control centres worldwide. This is a growing condition due to recent societal trends oriented towards the consumption of wild plants as food, cosmetics, or medicine. At least three general causes of plant poisoning can be identified: plant misident...
journal_title：International journal of legal medicine
pub_type： 杂志文章,评审
doi：10.1007/s00414-016-1460-y
更新日期：2017-01-01 00:00:00
abstract：:The utility of postmortem microbiology has continuously been a topic of controversy. The present study describes a case of fatal sepsis in a patient with systemic lupus erythematosus. Postmortem culture and genotyping analyses allowed us to identify Klebsiella pneumoniae as the cause of sepsis, revealing the inadequat...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s00414-015-1160-z
更新日期：2015-09-01 00:00:00
abstract：:Scars of human skin can on occasions provide a very useful ancillary method of identification of an unknown deceased. If the age of any visible scars can be estimated objectively, then this might be of some assistance in the identification procedure. Melanocytes migrate into scar tissue as it ages and their number wit...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/s004140100225
更新日期：2001-10-01 00:00:00
abstract：:The localization of ABH related antigens in human male reproductive tract was examined using monoclonal antibodies and an avidin biotin complex method. No positive reaction with blood group antibodies on spermatozoa was observed in testis and ductus epididymidis apart from erythrocytes and endothelial cells. The expre...
journal_title：International journal of legal medicine
pub_type： 杂志文章
doi：10.1007/BF02340827
更新日期：1992-01-01 00:00:00
