Abstract:
:This study is a first approach to identify UVA-related alterations in situ of bovine eye lens proteins from the water-soluble and urea-soluble fractions upon aging. The fractions were obtained from irradiated long-term organ culture lenses and analyzed by mini two-dimensional gel electrophoresis. This micropreparative method followed by computer analysis allows high resolution and separation of microgram quantities of proteins and to detect spots which arose as a consequence of irradiation. To facilitate the analysis we first separated the water-soluble fraction into the major crystallin classes by gel filtration. Moreover, we immunoblotted the gel of the urea-soluble fraction with a specific antibody against the intermediate filament protein vimentin. Upon irradiation of young and adult lenses, alphaA-crystallin and vimentin showed obvious modifications. During aging the susceptibility to irradiation increased when vimentin started to degrade, whereas deamidation of alphaA-crystallin seems to occur.
journal_name
Ophthalmic Resjournal_title
Ophthalmic researchauthors
Weinreb O,van Rijk FA,Steely HT,Dovrat A,Bloemendal Hdoi
10.1159/000055613subject
Has Abstractpub_date
2000-09-01 00:00:00pages
195-204issue
5eissn
0030-3747issn
1423-0259pii
55613journal_volume
32pub_type
杂志文章abstract:BACKGROUND:The aim of this study is to provide a quantitative evaluation of the blood-aqueous barrier (BAB) in patients with ocular blunt trauma and evaluate its association with intraocular pressure (IOP) elevation. METHODS:This is a prospective case-control study, and the following 3 groups were included: elevated I...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000445352
更新日期:2016-07-01 00:00:00
abstract::A method for microscopical observation and quantification of corneal neovascularization in the living mouse is described. Measurements of the corneal vasculature were made on still-mode video-pictures of the cornea. The validity of the method was shown by the high correlation between the morphometric measurements and ...
journal_title:Ophthalmic research
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journal_title:Ophthalmic research
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更新日期:2020-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Ophthalmic research
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267836
更新日期:1995-01-01 00:00:00
abstract::Autofluorescence and transmission values of the lens for blue-green light were determined at two locations with the use of identical fluorophotometers: in Coimbra, Portugal, in 44 healthy controls, 15 haemodialysis and 11 renal failure patients, and in Leiden, The Netherlands, in 57 healthy controls, 23 haemodialysis ...
journal_title:Ophthalmic research
pub_type: 杂志文章
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更新日期:1988-01-01 00:00:00
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更新日期:2011-01-01 00:00:00
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journal_title:Ophthalmic research
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更新日期:1994-01-01 00:00:00
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journal_title:Ophthalmic research
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更新日期:2020-04-30 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267895
更新日期:1996-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000055595
更新日期:2000-03-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章,评审
doi:10.1159/000055530
更新日期:1999-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章,评审
doi:10.1159/000316477
更新日期:2010-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000055694
更新日期:2001-11-01 00:00:00
abstract::Diabetic macular edema is considered the most important factor related to visual impairment in patients with diabetic retinopathy. Together with the use of grid and focal laser photocoagulation, today the intravitreal administration of pharmacotherapies represents the standard of care for the treatment of this complic...
journal_title:Ophthalmic research
pub_type: 杂志文章,评审
doi:10.1159/000362764
更新日期:2014-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265442
更新日期:1986-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
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更新日期:2017-01-01 00:00:00
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更新日期:2016-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-01-01 00:00:00
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更新日期:2012-01-01 00:00:00
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更新日期:1983-01-01 00:00:00
abstract:BACKGROUND:To assess the cytotoxic properties of voriconazole and sulfobutylether-β-cyclodextrin (SBECD) on cultured primary human corneal epithelial cells. METHODS:Human corneal epithelial cells were cultured and exposed to various concentrations of SBECD (0.016-32 mg/ml) and voriconazole (0.001-2 mg/ml). Cellular cy...
journal_title:Ophthalmic research
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
abstract::Inflammation is substantially contributing to the development and worsening of diabetic retinopathy in general and diabetic macular edema (DME) in particular, which provides the rationale to treat DME with corticosteroids. While anti-vascular endothelial growth factor (VEGF) agents are mostly chosen as a first-line tr...
journal_title:Ophthalmic research
pub_type: 杂志文章,评审
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更新日期:2019-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000048333
更新日期:2002-03-01 00:00:00
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journal_title:Ophthalmic research
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doi:10.1159/000090268
更新日期:2006-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00