Abstract:
:We have been studying oligodendrocyte generation in vitro to obtain insights into how the timely generation of these cells might be regulated. Our studies suggest the existence of timing mechanisms quite different from those of existing models, wherein it is proposed that timely oligodendrocyte generation is associated with synchronous and symmetric differentiation controlled by cell-intrinsic biological clocks. Our results are most consistent with the hypothesis that the propensity of a clone of dividing oligodendrocyte type-2 astrocyte (O-2A) progenitors initially to generate at least one oligodendrocyte may be regulated by cell-intrinsic mechanisms, but that cell-extrinsic signals regulate the extent of further oligodendrocyte generation. In cultures of embryonic rat cortex grown in the presence of platelet-derived growth factor (PDGF), oligodendrocytes appeared in a timely manner in the absence of clonal differentiation. In contrast with previous suggestions, the presence or absence of thyroid hormone (T3) did not alter the probability of individual clones of O-2A progenitors generating at least one oligodendrocyte in vitro at a time equal to the rat's day of birth. Instead, T3 increased the proportion of oligodendrocytes generated within clones. For postnatally derived progenitor cells, the initial appearance of oligodendrocytes also was followed by further asymmetric generation of these cells, with the ratio of progenitors to oligodendrocytes within clones being regulated by environmental signals. T3 and ciliary neurotrophic factor increased oligodendrocyte generation, while neurotrophin-3 (NT-3) suppressed oligodendrocyte generation. Also in contrast to previous reports, NT-3 was not required for the promotion of extensive division of O-2A progenitor cells by PDGF.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Ibarrola N,Mayer-Pröschel M,Rodriguez-Peña A,Noble Mdoi
10.1006/dbio.1996.0280subject
Has Abstractpub_date
1996-11-25 00:00:00pages
1-21issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(96)90280-8journal_volume
180pub_type
杂志文章abstract::Fetomodulin (FM) was previously shown to be a surface marker protein of parietal endoderm by in vitro differentiation of F9 embryonal carcinoma cells and by immunohistochemistry of in vivo embryos. BALB/3T3 and sarcoma S180 cells of the mouse were also shown to possess a protein which was indistinguishable from FM by ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90058-q
更新日期:1990-07-01 00:00:00
abstract::Fibroblast growth factor (FGF) signalling has been implicated in the generation of mesoderm and neural fates in chordate embryos including ascidians and vertebrates. In Ciona, FGF9/16/20 has been implicated in both of these processes. However, in FGF9/16/20 knockdown embryos, notochord fate recovers during later devel...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.08.075
更新日期:2007-02-01 00:00:00
abstract::Trichosia pubescens larvae were ligated at different prepupal developmental stages in order to investigate whether ecdysterone acts just as a trigger or whether its presence in the hemolymph is constantly necessary for the induction of the late puffs, in particular the DNA puffs. The larvae were ligated in the middle ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90170-2
更新日期:1984-03-01 00:00:00
abstract::The PITX1 transcription factor is expressed during hindlimb development, where it plays a critical role in directing hindlimb growth and the specification of hindlimb morphology. While it is known that PITX1 regulates hindlimb formation, in part, through activation of the Tbx4 gene, other transcriptional targets remai...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.12.013
更新日期:2018-02-01 00:00:00
abstract::In the marine bivalve Hiatella flaccida, full-grown oocytes in ovaries are arrested at the first prophase (prophase-I) of meiosis, whereas spawned oocytes have reinitiated meiosis from prophase-I and are again arrested at the first metaphase (metaphase-I). The neurohormone serotonin (5-hydroxytryptamine, 5-HT) was abl...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1298
更新日期:1995-10-01 00:00:00
abstract::We describe the characterization and a functional analysis in Xenopus development of RalB, a small G protein. RalB RNA and protein are detectable during oogenesis and early development, but the gene is expressed only weakly in adult tissues. The RalB transcripts are processed by poly(A) extension during oocyte maturat...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9254
更新日期:1999-05-15 00:00:00
abstract::Ca2+-sensitive microelectrodes were used to study changes in cytoplasmic free calcium during progesterone-induced meiotic maturation in Xenopus laevis oocytes. In contrast to previous reports, no changes were detected during the maturation process, although a large activation pulse of Ca2+ was seen when in vitro matur...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90475-0
更新日期:1985-06-01 00:00:00
abstract::A group of proteins judged on several criteria to be yolk proteins have been isolated from a homogenate of the nematode Caenorhabditis elegans. Comparison of partial proteolysis fragments indicates that the two bands of a 170,000-dalton doublet (yp170) are closely related; bands observed at 115,000 daltons (yp115) and...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90321-4
更新日期:1983-03-01 00:00:00
abstract::The C-X-C motif ligand 14 (CXCL14) is a recently discovered chemokine that is highly conserved in vertebrates and expressed in various embryonic and adult tissues. CXCL14 signaling has been implicated to function as an antiangiogenic and anticancer agent in adults. However, its function during development is unknown. ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.01.008
更新日期:2017-03-01 00:00:00
abstract::The lateral line system displays highly divergent patterns in adult teleost fish. The mechanisms underlying this variability are poorly understood. Here, we demonstrate that the lateral line mechanoreceptor, the neuromast, gives rise to a series of accessory neuromasts by a serial budding process during postembryonic ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.02.017
更新日期:2010-04-15 00:00:00
abstract::Previous studies have established that embryonal carcinoma (EC) cells produce several different growth factors, but express few, if any, receptors for epidermal growth factor, platelet-derived growth factor, or transforming growth factor type-beta. In this study, the production and utilization of fibroblast growth fac...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90161-3
更新日期:1988-09-01 00:00:00
abstract::Nerve and muscle development was studied in paralysé mutant mice. The mutant phenotype is first recognizable 6-7 days after birth (PN 6-PN 7) as cessation of muscle growth and weakness and incoordination of movement. Mutant animals die between 2 and 3 weeks of age. Muscle fibers from paralysé mutants had a unimodal di...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90213-3
更新日期:1989-03-01 00:00:00
abstract::Serotonin is a classical small-molecule neurotransmitter with known effects on developmental processes. Previous studies have shown a developmental role for serotonin in the fly peripheral nervous system. In this study, we show that serotonin can modulate the development of serotonergic varicosities within the fly cen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.07.025
更新日期:2005-10-01 00:00:00
abstract::Retinoic acid (RA) is a potent inducer of cell differentiation and plays an essential role in sex-specific germ cell development in the mammalian gonad. RA is essential for male gametogenesis and hence fertility. However, RA can also disrupt sexual cell fate in somatic cells of the testis, promoting transdifferentiati...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.02.015
更新日期:2017-04-15 00:00:00
abstract::Skeletal muscle fibers express members of the myosin heavy chain (MyHC) gene family in a fiber-type-specific manner. In avian skeletal muscle it is the expression of the slow MyHC isoforms that most clearly distinguishes slow- from fast-contracting fiber types. Two hypotheses have been proposed to explain fiber-type-s...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8619
更新日期:1997-08-01 00:00:00
abstract::Notch signaling inhibits hair cell differentiation, based on studies on mice deficient in Notch signaling-related genes and its downstream genes. However, the precise mechanisms of this inhibition are unknown because it is difficult to control the timing and duration of the suppression of Notch signaling. Here, we dev...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.04.035
更新日期:2007-07-01 00:00:00
abstract::The effect of phenylephrine, an alpha-1 adrenergic agonist, on development of body asymmetry was studied using a rat whole embryo culture system. Embryos were explanted at the presomite stage, cultured in 100% rat serum containing various concentrations of phenylephrine, and examined at the 20-25 somite stage for side...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90067-d
更新日期:1991-01-01 00:00:00
abstract::Hoxa2 gene is a primary player in regulation of craniofacial programs of head development in vertebrates. Here we investigate the evolution of a Hoxa2 neural crest enhancer identified originally in mouse by comparing and contrasting the fugu hoxa2a and hoxa2b genes with their orthologous teleost and mammalian sequence...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.11.006
更新日期:2016-01-15 00:00:00
abstract::These studies were designed to test the hypothesis that sperm nuclear decondensation and male pronuclear formation during hamster fertilization depend upon the ability of the fertilized oocyte to reduce sperm nuclear disulfide bonds. In a first series of experiments, treatment of mature oocytes with the sulfhydryl blo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90126-x
更新日期:1984-01-01 00:00:00
abstract::Salivary gland formation in the Drosophila embryo is dependent on the homeotic gene Sex combs reduced (Scr). When Scr function is missing, salivary glands do not form, and when SCR is expressed everywhere in the embryo, salivary glands form in new places. Scr is normally expressed in all the cells that form the saliva...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9560
更新日期:2000-01-15 00:00:00
abstract::We have previously reported the detection of cAMP waves within monolayers of aggregating Dictyostelium discoideum cells (K. J. Tomchik and P.N. Devreotes, 1981, Science 212, 443-446). The computer-assisted analysis presented here of the fluorographic images of the cAMP waves reveals (1) all the waves have a consistent...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90178-1
更新日期:1983-04-01 00:00:00
abstract::The homebox gene Hlxb9, encoding Hb9, exhibits a dual expression profile during pancreatic development. The early expression in the dorsal and ventral pancreatic epithelium is transient and spans from embryonic day (e) 8 to e9-e10, whereas the later expression is confined to differentiating beta-cells as they appear. ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0440
更新日期:2001-12-01 00:00:00
abstract::Vascular smooth muscle of the head derives from neural crest, but developmental mechanisms and early transcriptional drivers of the vSMC lineage are not well characterized. We find that in early development, the transcription factor foxc1b is expressed in mesenchymal cells that associate with the vascular endothelium....
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.06.005
更新日期:2019-09-01 00:00:00
abstract::Huntington disease (HD) is an adult-onset neurodegenerative disease characterized by motor, cognitive, and psychiatric symptoms that is caused by a CAG expansion in the HTT gene. Palmitoylation is the addition of saturated fatty acids to proteins by DHHC palmitoylacyl transferases. HTT is palmitoylated by huntingtin i...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.11.018
更新日期:2015-01-15 00:00:00
abstract::pmar1 is a transcription factor in the paired class homeodomain family that was identified and found to be transcribed in micromeres beginning at the fourth cleavage of sea urchin development [Dev. Biol. 246 (2002), 209]. Based on in situ data, molecular perturbation studies, and QPCR data, the recently published gene...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00108-8
更新日期:2003-06-01 00:00:00
abstract::Caenorhabditis elegans embryonic elongation depends on both epidermal and muscle cells. The hemidesmosome-like junctions, commonly called fibrous organelles (FOs), that attach the epidermis to the extracellular matrix ensure muscle anchoring to the cuticular exoskeleton and play an essential role during elongation. To...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.11.025
更新日期:2011-02-15 00:00:00
abstract::To examine whether the 200-kDa neurofilament protein (200K NFP) is involved in mechanically stabilizing axons, we studied the developmental appearance of immunoreactivity to nonphosphorylated and phosphorylated 200K NFP at the neuromuscular junction. Polyinnervated rat muscle fibers become singly innervated during the...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90422-8
更新日期:1988-11-01 00:00:00
abstract::Ammonium transporter C (AmtC) is one of three transporters in Dictyostelium that have been proposed to regulate entry and exit of ammonia in a cell type dependent manner and to mediate ammonia signaling. Previous work demonstrated that disruption of the amtC gene results in a slugger phenotype in which the cells remai...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.08.043
更新日期:2005-11-01 00:00:00
abstract::NK genes are related pan-metazoan homeobox genes. In the fruitfly, NK genes are clustered and involved in patterning various mesodermal derivatives during embryogenesis. It was therefore suggested that the NK cluster emerged in evolution as an ancestral mesodermal patterning cluster. To test this hypothesis, we cloned...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.02.013
更新日期:2008-05-15 00:00:00
abstract::An understanding of heart development is critical in any systems biology approach to cardiovascular disease. The interpretation of data generated from high-throughput technologies (such as microarray and proteomics) is also essential to this approach. However, characterizing the role of genes in the processes underlyi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.03.011
更新日期:2011-06-01 00:00:00