Abstract:
:We compared three drugs representing different classes of antidiabetic pharmacology (glyburide, a sulfonylurea; pioglitazone, a thiazolidinedione; and metformin, a biguanide) in terms of their direct effects on proliferation of cultured arterial smooth muscle cells (SMC). Rat aortic SMC were seeded at 4 x 10(4)/35 mm well. After 24 h, they were treated every 2 to 4 days for 2 weeks with 5% fetal bovine serum (FBS) in normal culture medium containing either drug vehicles or a low and a high but nontoxic level of glyburide (0.5 and 2.5 mumol/L), pioglitazone (1 and 5 mumol/L), and metformin (20 and 100 mumol/L). Vehicle-treated cells increased from 2 +/- 0 to 6 +/- 1 to 42 +/- 3 to 210 +/- 14 (cells per well x 10(4); 5 wells each) from day zero to 4 to 9 to 14. From day 9 to 14 these cell numbers were decreased an average of 20% by the 2.5 mumol/L glyburide (P < .05) and 43% by the 5 mumol/L pioglitazone (P < .05). The low levels of glyburide and pioglitazone and both the low and high levels of metformin failed to influence cell numbers. In a second experiment, even half the abovementioned high level of pioglitazone (2.5 mumol/L) still exerted a markedly greater antiproliferative effect on aortic SMC than a high level of 2.0 mumol/L glyburide (P < .05). In addition, neither drug's antiproliferative effect was influenced by a high level of insulin added to the medium (10 mU/mL). Similarly, a small but significant stimulatory effect of this high insulin on cell proliferation (P < .05) was not significantly affected by these two drugs (although pioglitazone tended to inhibit it). These results suggest that thiazolidinediones may be more useful antidiabetic agents than sulfonylureas and biguanides in inhibiting abnormal arterial SMC proliferation associated with atherosclerosis and postangioplastic restenosis which are common in diabetic patients.
journal_name
Am J Hypertensjournal_title
American journal of hypertensionauthors
Peuler JD,Phare SM,Iannucci AR,Hodorek MJdoi
10.1016/0895-7061(95)00393-2subject
Has Abstractpub_date
1996-02-01 00:00:00pages
188-92issue
2eissn
0895-7061issn
1941-7225pii
0895706195003932journal_volume
9pub_type
杂志文章abstract:BACKGROUND:Although several characteristics of aortic function, which are largely determined by age, predict outcomes beyond brachial blood pressure (BP), the extent to which brachial BP control accounts for age-related variations in aortic function is uncertain. We aimed to determine the extent to which brachial BP co...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1093/ajh/hpv140
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND:During the development of hypertension, glucose replaces triacylglycerols (TG) as the main energy source for the myocardium. However, there are no available studies investigating the TG molecular species composition of the myocardium in the spontaneously hypertensive rat (SHR). The objective of this study wa...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1016/j.amjhyper.2004.11.012
更新日期:2005-01-01 00:00:00
abstract::The role of an endogenous sodium pump inhibitor in the pathogenesis of hypertension has been reported in several papers. Unfortunately, because of the unknown structure and lack of biochemical characterization, some discrepancies have arisen. In this study we report a method to obtain extracts from human plasma that a...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1093/ajh/1.3.56s
更新日期:1988-07-01 00:00:00
abstract:BACKGROUND:In hypertensives, nondippers are more likely than dippers to suffer silent, as well as overt, hypertensive target organ damage. In this study, we investigated whether a nondipper status was associated with target organ damage in normotensives. METHODS:We performed ambulatory blood pressure (BP) monitoring, ...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1016/s0895-7061(03)00567-3
更新日期:2003-06-01 00:00:00
abstract:BACKGROUND:Previous autopsy studies suggested that a reduced nephron number is associated with increased risk of hypertension and chronic kidney disease. However, the significance of the nephron number estimated from a renal biopsy in patients with hypertensive nephrosclerosis (HNS) has not yet been elucidated. METHOD...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1093/ajh/hpu267
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND:Altered diastolic filling is an important contributor to several cardiovascular disorders. Multiple lines of evidence suggest a genetic contribution to left ventricular (LV) diastolic filling; however, chromosomal locations harboring genes involved in impaired LV diastolic filling have not been reported. The...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1016/s0895-7061(02)02943-6
更新日期:2002-07-01 00:00:00
abstract::The hypertensive transgenic rat model TGR(mRen2)27 has been used to investigate the development of cardiac and vascular hypertrophy in response to two different drug regimes. Cardiac hypertrophy was shown to be related to age and gender with the copy number of mouse renin transgenes having an additive effect. A simila...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1016/s0895-7061(99)00021-7
更新日期:1999-07-01 00:00:00
abstract:BACKGROUND:Recent studies have revealed the existence of bone marrow-derived endothelial progenitor cells (EPCs). The number of circulating EPCs might reflect the pathogenesis of atherosclerosis and progression of cardiovascular diseases (CVDs). The purpose of this study was to evaluate the relationship between the num...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1038/ajh.2008.278
更新日期:2008-11-01 00:00:00
abstract::Increased dietary sodium intake has been associated with an increase in blood pressure as well as a decrease in insulin-mediated glucose disposal in young healthy adults. The purpose of this study was to determine whether dietary sodium intake is associated with changes in oral glucose tolerance, insulin sensitivity, ...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1016/s0895-7061(97)00159-3
更新日期:1997-09-01 00:00:00
abstract::Intracellular free sodium levels ([Na+]i) were assessed with 23Na nuclear magnetic resonance (NMR) spectroscopy in isolated, Langendorff-perfused normal, compensated hypertrophied, and hypertrophied failing guinea pig hearts under several conditions. Baseline [Na+]i measured with a shift reagent was significantly grea...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1016/0895-7061(95)00219-F
更新日期:1995-09-01 00:00:00
abstract::The "totality" of hypertension clinical trial endpoint data has shown that the absolute benefit of pharmacological blood pressure (BP) lowering is directly related to the BP level and baseline cardiovascular risk, albeit with attenuation of the relative risk reduction per unit of BP lowering in patients with diabetes ...
journal_title:American journal of hypertension
pub_type: 杂志文章,评审
doi:10.1093/ajh/hpw087
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Hypertension is a significant global public health challenge. Low-grade inflammation is known to facilitate the development of essential hypertension and target-organ hypertensive damage. Neutrophil to lymphocyte ratio (NLR) is a simple and reliable indicator of inflammation that may also be useful in the pr...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1093/ajh/hpv034
更新日期:2015-11-01 00:00:00
abstract::Blood pressure is reportedly elevated in the spontaneously hypertensive rat (SHR) neonate, the etiology of which remains unclear. Aberrations in the hypothalamic-pituitary-adrenal axis have been implicated, as it is well accepted that excess corticosteroids are associated with hypertension. We examined aspects of adre...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1016/s0895-7061(99)00010-2
更新日期:1999-05-01 00:00:00
abstract:BACKGROUND:To determine all-cause and circulatory system (cardiovascular)-related mortality in subjects with different grades of hypertension, we performed a prospective study. METHODS:A total of 148,173 individuals aged > or =35 years were recruited in Mumbai, India in years 1991-1997. Clinical history and anthropome...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1038/ajh.2009.131
更新日期:2009-10-01 00:00:00
abstract::The aim of this study was to compare the efficacy (lowering diastolic blood pressure to 90 mm Hg or less) of urapidil, a postsynaptic alpha-blocker with central action with that of captopril in a randomized, double-blind, multicenter study. Two hundred ninety-five essential hypertensives (WHO I/II) (140 male, 155 fema...
journal_title:American journal of hypertension
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1093/ajh/1.3.233s
更新日期:1988-07-01 00:00:00
abstract::Several classes of antihypertensive drugs have been shown to improve vascular function through mechanisms other than reducing blood pressure (BP) alone. Certain dihydropyridine calcium channel blockers (CCBs) and inhibitors of the renin-angiotensin system (RAS) increase nitric oxide (NO) bioavailability and decrease o...
journal_title:American journal of hypertension
pub_type: 杂志文章,评审
doi:10.1038/ajh.2008.258
更新日期:2008-10-01 00:00:00
abstract::In normal subjects, insulin decreases the urinary excretion of sodium, potassium, and uric acid. We tested whether these renal effects of insulin are altered in insulin resistant hypertension. In 37 patients with essential hypertension, we measured the changes in urinary excretion of sodium, potassium, and uric acid i...
journal_title:American journal of hypertension
pub_type: 临床试验,杂志文章
doi:10.1016/0895-7061(96)00098-2
更新日期:1996-08-01 00:00:00
abstract:BACKGROUND:Nocturnal hypertension is an important phenotype of abnormal diurnal blood pressure (BP) variability and a known risk marker for target organ damage and cardiovascular events. This study aimed to assess the differential BP-lowering effects of esaxerenone vs. eplerenone on nocturnal BP in hypertensive patient...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1093/ajh/hpaa155
更新日期:2020-11-09 00:00:00
abstract:OBJECTIVE:We tested the hypothesis that repetitive severe hypoxemia resulting from obstructive sleep apnea would increase serum erythropoietin, and that this increase would be attenuated by effective treatment of obstructive sleep apnea. METHODS:We studied healthy untreated patients with obstructive sleep apnea (18 se...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1016/j.amjhyper.2004.04.011
更新日期:2004-09-01 00:00:00
abstract:BACKGROUND:Azilsartan medoxomil (AZL-M), an angiotensin II receptor blocker, demonstrates antihypertensive and organ protective effects in hypertension. We investigated the efficacy of AZL-M to ameliorate metabolic syndrome and kidney damage associated with type 2 diabetes using Zucker diabetic fatty (ZDF) rats. METHO...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1093/ajh/hpu016
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND:Blood pressure (BP) measured in the office setting increases from early through later adulthood. However, it is unknown to what extent out-of-office BP derived via ambulatory BP monitoring (ABPM) increases over time, and which participant characteristics and risk factors might contribute to these increases. ...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1093/ajh/hpaa189
更新日期:2020-11-17 00:00:00
abstract::The effects of activators and inhibitors of protein kinase C (PKC) on the production of C-type natriuretic peptide (CNP) by cultured human aortic endothelial cells were examined. The PKC activators phorbol 12-myristate 13-acetate (PMA) and 1-oleoyl 2-acetyl glycerol (OAG) stimulated CNP release; the maximal effects we...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1016/s0895-7061(96)00107-0
更新日期:1996-09-01 00:00:00
abstract:BACKGROUND:Increased short-term blood pressure (BP) variability (BPV) in hemodialysis is associated with increased cardiovascular and all-cause mortality. Studies on the impact of BP-lowering interventions on BPV are scarce. This study examined the effect of dry-weight reduction with a lung ultrasound-guided strategy o...
journal_title:American journal of hypertension
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1093/ajh/hpz064
更新日期:2019-07-17 00:00:00
abstract:BACKGROUND:There is evidence from genome wide association study that single-nucleotide polymorphisms (SNPs) in the 5' end of the uromodulin gene (UMOD) affect uromodulin excretion and blood pressure (BP). Uromodulin is almost exclusively expressed in the thick ascending limb of the loop of Henle (TAL) and its effect on...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1093/ajh/hpaa166
更新日期:2020-10-21 00:00:00
abstract:BACKGROUND:The W allele of the G460W polymorphism in the adducin-1 gene has been occasionally associated with increased blood pressure (BP). The aim of this study was to test whether the G460W variant is associated with BP levels and BP progression rate and whether G460W associations with BP are affected by sex, body m...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1016/j.amjhyper.2007.04.007
更新日期:2007-09-01 00:00:00
abstract::The results of our previous studies suggested that endothelin-1 (ET-1) might be contributory to the impaired uteroplacental blood flow seen in preeclampsia. The aim of this study was to investigate the in vitro influence of isradipine on ET-1-induced contraction of myometrial resistance arteries from pregnant women, a...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1093/ajh/7.7.50s
更新日期:1994-07-01 00:00:00
abstract::Cicletanine sulfate was tested on bicarbonate-dependent pHi changes in cultured vascular smooth muscle (A10 line). Cicletanine sulfate exhibited double reactivity with regard to the cell alkalinization induced by bicarbonate uptake. The analysis of 11 concentration-response curves revealed a high reactivity component ...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1093/ajh/6.8.674
更新日期:1993-08-01 00:00:00
abstract::The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is a new genetic model of non-insulin-dependent diabetes mellitus (NIDDM). We investigated whether treatment with an angiotensin II (ANGII) subtype-1 receptor antagonist delays the onset of NIDDM and attenuates diabetic nephropathy in the OLETF rat. OLETF rats fed a re...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1016/s0895-7061(98)00276-3
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:Marinobufagenin (MBG) is an endogenous Na/K-ATPase inhibitor, a natriuretic and a vasoconstrictor. MBG is implicated in salt-sensitive hypertension, cardiac hypertrophy, and initiate the pro-fibrotic signaling. Previously it was demonstrated that immunoneutralization of an endogenous MBG by 3E9 anti-MBG-anti...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1093/ajh/hpv155
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND:Pulse pressure, a measure of central arterial stiffness and a predictor of cardiovascular mortality, has known genetic components. METHODS:To localize the genetic effects of pulse pressure, we conducted a genome-wide linkage analysis of 1,892 American-Indian participants of the Strong Heart Family Study (SH...
journal_title:American journal of hypertension
pub_type: 杂志文章
doi:10.1038/ajh.2007.34
更新日期:2008-02-01 00:00:00