Application of MS and NMR to the structure elucidation of complex sugar moieties of natural products: exemplified by the steroidal saponin from Yucca filamentosa L.

abstract：:The cuticle of higher plants functions primarily as a protective barrier for the leaves and fruits, controlling microbial attack as well as the diffusion of water and chemicals from the outside environment. Its major chemical constituents are waxes (for waterproofing) and cutin (a structural support polymer). However,...

journal_title：Phytochemistry

authors： Fang X,Qiu F,Yan B,Wang H,Mort AJ,Stark RE

更新日期：2001-07-01 00:00:00

abstract：:In plants, it has been proposed that hexacoordinate (class 1) non-symbiotic Hbs (nsHb-1) function in vivo as peroxidases. However, little is known about peroxidase activity of nsHb-1. We evaluated the peroxidase activity of rice recombinant Hb1 (a nsHb-1) by using the guaiacol/H2O2 system at pH 6.0 and compared it to ...

journal_title：Phytochemistry

authors： Violante-Mota F,Tellechea E,Moran JF,Sarath G,Arredondo-Peter R

更新日期：2010-01-01 00:00:00

abstract：:Twenty phenolic compounds, representatives of proanthocyanidins and gallic acid/hexahydroxyldiphenic acid esters of glucose, have been assessed for their ability to inhibit binding of specific radioligands to 16 receptors. The receptors utilized were alpha 1-, alpha 2- and beta-adrenoceptors, adenosine 1, dopamine 1 a...

journal_title：Phytochemistry

authors： Zhu M,Phillipson JD,Greengrass PM,Bowery NE,Cai Y

更新日期：1997-02-01 00:00:00

abstract：:A flavonoid trioside and its coumaryl ester together with seven known flavonoids and five phenolic acids were isolated from the leaves of Reseda muricata. Their structures were elucidated by spectroscopic methods including UV, FAB MS, 1H, 13C and 2D-NMR, DEPT, HMBC and HMQC experiments. The two compounds were identifi...

journal_title：Phytochemistry

authors： El-Sayed NH,Omara NM,Yousef AK,Farag AM,Mabry TJ

更新日期：2001-06-01 00:00:00

abstract：:Six cardenolides were isolated from the anti-tumor active fraction of the 75% ethanol extract of Streptocaulon juventas (Asclepiadaceae), mainly found in southwest of China. These were named 1α, 14β-dihydroxy-5β-card-20 (22)-enolide 3-O-[O-β-D-glucopyranosyl-(1→2)-β-D-digitalopyranoside] (1), acovenosigenin A 3-O-[O-β...

journal_title：Phytochemistry

authors： Xue R,Han N,Ye C,Wang HB,Yin J

更新日期：2013-04-01 00:00:00

abstract：:The role and fate of Jasmonoyl-Phenylalanine (JA-Phe), an understudied conjugate in the jasmonate pathway remain to be unraveled. We addressed here the possibility of JA-Phe oxidative turnover by cytochrome P450s of the CYP94 family. Leaf wounding or fungal infection in Arabidopsis resulted in accumulation of JA-Phe, ...

journal_title：Phytochemistry

authors： Widemann E,Grausem B,Renault H,Pineau E,Heinrich C,Lugan R,Ullmann P,Miesch L,Aubert Y,Miesch M,Heitz T,Pinot F

更新日期：2015-09-01 00:00:00

abstract：:Cannabis sativa L. is the source for a whole series of chemically diverse bioactive compounds that are currently under intensive pharmaceutical investigation. In this work, hot and cold water extracts as well as ethanol/water mixtures (tinctures) of cannabis were compared in order to better understand how these extrac...

journal_title：Phytochemistry

authors： Politi M,Peschel W,Wilson N,Zloh M,Prieto JM,Heinrich M

更新日期：2008-01-01 00:00:00

abstract：:In a chemotaxonomic survey of 57 Pelargonium species, leaf exudate flavonoids were detected in 35% of the sample, mostly in trace amounts. However, chrysin and a related C-methylflavanone were identified as major leaf surface constituents of P. crispum, and a mixture of quercetin and kaempferol mono-, and di- and trim...

journal_title：Phytochemistry

authors： Williams CA,Harborne JB,Newman M,Greenham J,Eagles J

更新日期：1997-12-01 00:00:00

abstract：:As a basis for future investigations of evolutionary trajectories and biosynthetic mechanisms underlying variations in glucosinolate structures, we screened members of the crucifer tribe Cardamineae by HPLC-MS/MS, isolated and identified glucosinolates by NMR, searched the literature for previous data for the tribe, a...

journal_title：Phytochemistry

authors： Olsen CE,Huang XC,Hansen CIC,Cipollini D,Ørgaard M,Matthes A,Geu-Flores F,Koch MA,Agerbirk N

更新日期：2016-12-01 00:00:00

abstract：:In search for new or chemo-taxonomically relevant bioactive compounds from chemically unexplored Hypericum species, four previously undescribed natural products, named peplidiforones A-D were isolated and characterized from Hypericum peplidifolium A. Rich., together with six known compounds. The structures of all comp...

journal_title：Phytochemistry

authors： Fobofou SA,Harmon CR,Lonfouo AH,Franke K,Wright SM,Wessjohann LA

更新日期：2016-04-01 00:00:00

abstract：:In a study of 62 plant species of the Cretan flora for their phytotoxic activity, plants were extracted successively with CH2Cl2, MeOH and H2O. Phytotoxicity evaluation of the 240 extracts was performed against Lactuca sativa L. and Agrostis stolonifera L.. The MeOH extract of Bellis longifolia was the most phytotoxic...

journal_title：Phytochemistry

authors： Stavropoulou MI,Angelis A,Aligiannis N,Kalpoutzakis E,Mitakou S,Duke SO,Fokialakis N

更新日期：2017-12-01 00:00:00

abstract：:Prenylation reactions contribute considerably to the diversity of natural products. Polyprenylated secondary metabolites include hyperforin which is both quantitatively and pharmacologically a major constituent of the medicinal plant Hypericum perforatum (St. John's wort). Cell cultures of the related species Hypericu...

journal_title：Phytochemistry

authors： Boubakir Z,Beuerle T,Liu B,Beerhues L

更新日期：2005-01-01 00:00:00

abstract：:Two bitter stigmastane derivatives, vernoguinosterol (1) and vernoguinoside (2), have been isolated from the stem bark of Vernonia guineensis and their structures eludicated using spectroscopic methods. The new compounds exhibit trypanocidal activity. ...

journal_title：Phytochemistry

authors： Tchinda AT,Tsopmo A,Tane P,Ayafor JF,Connolly JD,Sterner O

更新日期：2002-02-01 00:00:00

abstract：:Highly specialized chemical defense pathways are a particularly noteworthy metabolic characteristic of sessile organisms, whether terrestrial or marine, providing protection against pests and diseases. For this reason, knowledge of the metabolites involved in these processes is crucial to producing ecologically fit cr...

journal_title：Phytochemistry

authors： Pedras MS,To QH

更新日期：2015-05-01 00:00:00

abstract：:A sulfated fucan containing fraction (SmWE) was isolated from water extract of the brown seaweed Stoechospermum marginatum collected from the Arabian Sea. Anion exchange chromatography of the crude fraction results in the production of a sulfated fucan (F3) having a molecular mass of 40 kDa and specific rotation [alph...

journal_title：Phytochemistry

authors： Adhikari U,Mateu CG,Chattopadhyay K,Pujol CA,Damonte EB,Ray B

更新日期：2006-11-01 00:00:00

abstract：:Sugarcane neutral invertase (SNI) has been partially purified from mature sugarcane stem tissue to remove any potential competing activity. The enzyme is non-glycosylated and exhibits catalytic activity as a monomer, dimer and tetramer, most of the activity elutes as a monomer of native M(r) ca 60 k. The enzyme displa...

journal_title：Phytochemistry

authors： Vorster DJ,Botha FC

更新日期：1998-10-01 00:00:00

abstract：:Investigation of the aerial parts of Rhyncholacis penicillata afforded the new chromenes, 7-hydroxy-6-(3-methylbutyryl)-5-oxymethyl-chromene (rhynchonin A) and 7-hydroxy-6-(2-methylbutyryl)-6-oxymethylchromene (rhynchonin B). Structures were elucidated by spectroscopic methods and independent synthesis. Rhynchonin A s...

journal_title：Phytochemistry

authors： Burkhardt G,Becker H,Grubert M,Thomas J,Eicher T

更新日期：1994-12-01 00:00:00

abstract：:Psiadia arguta (Asteraceae) is endemic to the island of Mauritius in the Indian Ocean. The species is traditionally used to treat various ailments, such as its use as an expectorant or for the treatment of bronchitis and asthma. Preliminary biological screenings have displayed the antimalarial (Plasmodium falciparum) ...

journal_title：Phytochemistry

authors： Mahadeo K,Grondin I,Herbette G,Palama TL,Bouchemal N,Soulange J,Laulloo SJ,Sadeyen J,Humeau L,Frederich M,Gauvin-Bialecki A,Kodja H

更新日期：2020-08-01 00:00:00

abstract：:Phospholipase D (PLD) catalyzes the hydrolysis and transesterification of glycerophospholipids at the terminal phosphodiester bond. In many plants, several isoforms of PLD have been identified without knowing their functional differences. In this paper, the specificities of two PLD isoenzymes from white cabbage (Brass...

journal_title：Phytochemistry

authors： Dippe M,Ulbrich-Hofmann R

更新日期：2009-02-01 00:00:00

abstract：:Triterpene saponins 1-8 were isolated from the aerial parts of Gynostemma pubescens. Among them, compounds 1-4, 8 possess carboxylic groups at both C-21 and C-29, 5 contains a carboxylic group at C-21 and an aldehyde function at C-29, whereas 6 and 7 contain carboxylic groups at C-21 and hydroxymethylene groups at C-2...

journal_title：Phytochemistry

authors： Yang Z,Chen Q,Hu L

更新日期：2007-07-01 00:00:00

abstract：:Four new oleanane triterpenoid saponins named triplosides D-G were isolated from the roots of Triplostegia grandiflora. Their structures were elucidated on the basis of chemical degradation and spectral evidence. The saponins investigated were: oleanolic acid 3-O-beta-D-xylopyranosyl(1----4)-beta-D-xylopyranosyl(1----...

journal_title：Phytochemistry

authors： Ma WG,Wang DZ,Zeng YL,Yang CR

更新日期：1992-04-01 00:00:00

abstract：:The leaves of Cyclocarya paliurus with sweet taste are often used as herbal tea in People's Republic of China. In this study eight previously undescribed seco-dammarane type triterpenoids, cyclocariols A-H along with seven known compounds were isolated and characterized from its leaves. A possible biogenetic pathway f...

journal_title：Phytochemistry

authors： Chen YJ,Na L,Fan J,Zhao J,Hussain N,Jian YQ,Yuan H,Li B,Liu B,Choudhary MI,Khan I,Wang W

更新日期：2018-01-01 00:00:00

abstract：:Bispecific O-methyltransferase (OMT, EC 2.1.1.68) which catalyses the meta-specific methylation of caffeic acid and 5-hydroxyferulic acid was purified to homogeneity from the developing secondary xylem of aspen (Populus tremuloides). The enzyme was purified by conventional techniques and affinity chromatography on S-a...

journal_title：Phytochemistry

authors： Bugos RC,Chiang VL,Campbell WH

更新日期：1992-05-01 00:00:00

abstract：:A study of the chemistry and antibacterial activity of Scrophularia deserti led to the isolation of eight compounds, including the metabolite 3(zeta)-hydroxy-octadeca-4(E),6(Z)-dienoic acid (1). The known compounds ajugoside (2), scropolioside B (3), 6-O-alpha-L-rhamnopyranosylcatalpol (4), buddlejoside A(8) (5), scro...

journal_title：Phytochemistry

authors： Stavri M,Mathew KT,Gibbons S

更新日期：2006-07-01 00:00:00

abstract：:The methanol extract from Bellis perennis (Asteraceae) flowers was found to promote collagen synthesis in normal human dermal fibroblasts (NHDFs). Seven oleanane-type triterpene saponins, perennisosides XIII-XIX, and two known saponins, bellissaponins BS5 and BS9, were isolated from the methanol extract. The structure...

journal_title：Phytochemistry

authors： Morikawa T,Ninomiya K,Takamori Y,Nishida E,Yasue M,Hayakawa T,Muraoka O,Li X,Nakamura S,Yoshikawa M,Matsuda H

更新日期：2015-08-01 00:00:00

abstract：:Reinvestigation of the fermentation broth and mycelium of the coprophilous fungus Guanomyces polythrix, grown in static conditions, led to the isolation of several phytotoxic compounds, including two new naphthopyranone derivatives, namely (2S, 3R)-5-hydroxy-6,8-dimethoxy-2,3-dimethyl-2,3-dihydro-4H-naphtho[2,3-b]-pyr...

journal_title：Phytochemistry

authors： Macías M,Gamboa A,Ulloa M,Toscano RA,Mata R

更新日期：2001-11-01 00:00:00

abstract：:Chemical investigation of the vines and leaves of Momordica charantia resulted in isolation of fourteen cucurbitane triterpenoids, kuguacins F-S (1-14), including two pentanorcucurbitacins (6 and 7), one octanorcucurbitacin (8), and two trinorcucurbitacins (11 and 12), along with six known analogues. Their structures ...

journal_title：Phytochemistry

authors： Chen JC,Liu WQ,Lu L,Qiu MH,Zheng YT,Yang LM,Zhang XM,Zhou L,Li ZR

更新日期：2009-01-01 00:00:00

abstract：:This is the first study showing that alkaloids are present on the leaf surface of plants. A concentration of 30-230 pmol/cm2 pyrrolizidine alkaloids (PA's) was detected in 8 different samples taken from Senecio jacobaea. PA concentration on the leaves was marginally correlated with PA concentration of the total leaf t...

journal_title：Phytochemistry

authors： Vrieling K,Derridj S

更新日期：2003-12-01 00:00:00

abstract：:A high purity of β-D-glucan (80.8%) from hull-less barley (Hordeum vulgare L. var. nudum Hook. f.) (HBBG) was isolated by alkali extraction and multi-precipitation with ethanol. The molecular weight (Mw) of HBBG was determined as 571.4 kDa with a broad distribution (Mw/Mn = 1.6) by using HPSEC. According to methylatio...

journal_title：Phytochemistry

authors： Zhang H,Zhang N,Xiong Z,Wang G,Xia Y,Lai P,Ai L

更新日期：2018-11-01 00:00:00

abstract：:Two unprecedented guaiane-type sesquiterpene glycosides (undulatumosides A and B) were isolated by bioassay-guided fractionation from the MeOH extract of Pittosporum undulatum fruits, along with six known compounds, including the guaiane isomers 5-guaien-11-ol and 4-guaien-11-ol. The structures of the compounds were e...

journal_title：Phytochemistry

authors： Mendes SA,Mansoor TA,Rodrigues A,Armas JB,Ferreira MJ

更新日期：2013-11-01 00:00:00