Structural characterization and rheological properties of β-D-glucan from hull-less barley (Hordeum vulgare L. var. nudum Hook. f.).

Abstract:

:A high purity of β-D-glucan (80.8%) from hull-less barley (Hordeum vulgare L. var. nudum Hook. f.) (HBBG) was isolated by alkali extraction and multi-precipitation with ethanol. The molecular weight (Mw) of HBBG was determined as 571.4 kDa with a broad distribution (Mw/Mn = 1.6) by using HPSEC. According to methylation and GC-MS analysis, HBBG was identified to be composed of (1 → 4)- and (1 → 3)-glucopyranosyl (Glcp) residues with a ratio of (3.19 ± 0.01). The MALDI-TOF MS and NMR spectroscopy were further conducted to analyze the enzyme hydrolysate released by lichenase digestion on HBBG. The results suggested that HBBG possessed a typical chemical structure of cereal β-D-glucans, namely linear homopolysaccharides formed by β-D-Glcp units via (1 → 4)-linkages and occasionally single (1 → 3)-linkage. The trisaccharide and tetrasaccharide of HBBG accounted for 66.6% of total cellulosyl units, accompanying with a ratio of cellotriosyl to cellotetraosyl units = 1.0, which were significant different from those reported for the other cereal β-glucans. Rheological property analysis revealed that HBBG showed a shear-thinning behavior and thermal resilience during heating-cooling process.

journal_name

Phytochemistry

journal_title

Phytochemistry

authors

Zhang H,Zhang N,Xiong Z,Wang G,Xia Y,Lai P,Ai L

doi

10.1016/j.phytochem.2018.08.004

subject

Has Abstract

pub_date

2018-11-01 00:00:00

pages

155-163

eissn

0031-9422

issn

1873-3700

pii

S0031-9422(18)30458-8

journal_volume

155

pub_type

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