Involvement of calcium in ACC-oxidase activity from Cicer arietinum seed embryonic axes.

Abstract:

:Both in vivo and in vitro ACC-oxidase activities as well as ethylene production from embryonic axes of chickpea seeds were strongly inhibited by EGTA, a selective extracellular Ca2+ ion chelator, indicating that the influx of Ca2+ is important for enzymatic activity. EGTA inhibition was restored by exogenous Ca2+. Treatments of embryonic axes with either Verapamil and LaCl3 (both Ca2+ channel blockers) or TMB-8 (an intracellular Ca2+ antagonist) provoked an inhibition of both ACC-oxidase activity and ethylene production. These results suggest an involvement of calcium fluxes and intracellular calcium levels in the activity of the last step of the ethylene biosynthetic pathway, which is, in turn, intimately correlated with germination of Cicer arietinum seeds.

journal_name

Phytochemistry

journal_title

Phytochemistry

authors

Gallardo M,Gómez-Jiménez MC,Matilla A

doi

10.1016/s0031-9422(98)00591-3

subject

Has Abstract

pub_date

1999-02-01 00:00:00

pages

373-6

issue

3

eissn

0031-9422

issn

1873-3700

pii

S0031942298005913

journal_volume

50

pub_type

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