Abstract:
:Both in vivo and in vitro ACC-oxidase activities as well as ethylene production from embryonic axes of chickpea seeds were strongly inhibited by EGTA, a selective extracellular Ca2+ ion chelator, indicating that the influx of Ca2+ is important for enzymatic activity. EGTA inhibition was restored by exogenous Ca2+. Treatments of embryonic axes with either Verapamil and LaCl3 (both Ca2+ channel blockers) or TMB-8 (an intracellular Ca2+ antagonist) provoked an inhibition of both ACC-oxidase activity and ethylene production. These results suggest an involvement of calcium fluxes and intracellular calcium levels in the activity of the last step of the ethylene biosynthetic pathway, which is, in turn, intimately correlated with germination of Cicer arietinum seeds.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Gallardo M,Gómez-Jiménez MC,Matilla Adoi
10.1016/s0031-9422(98)00591-3subject
Has Abstractpub_date
1999-02-01 00:00:00pages
373-6issue
3eissn
0031-9422issn
1873-3700pii
S0031942298005913journal_volume
50pub_type
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