Multivariate approaches in plant science.


:The objective of proteomics is to get an overview of the proteins expressed at a given point in time in a given tissue and to identify the connection to the biochemical status of that tissue. Therefore sample throughput and analysis time are important issues in proteomics. The concept of proteomics is to encircle the identity of proteins of interest. However, the overall relation between proteins must also be explained. Classical proteomics consist of separation and characterization, based on two-dimensional electrophoresis, trypsin digestion, mass spectrometry and database searching. Characterization includes labor intensive work in order to manage, handle and analyze data. The field of classical proteomics should therefore be extended to also include handling of large datasets in an objective way. The separation obtained by two-dimensional electrophoresis and mass spectrometry gives rise to huge amount of data. We present a multivariate approach to the handling of data in proteomics with the advantage that protein patterns can be spotted at an early stage and consequently the proteins selected for sequencing can be selected intelligently. These methods can also be applied to other data generating protein analysis methods like mass spectrometry and near infrared spectroscopy and examples of application to these techniques are also presented. Multivariate data analysis can unravel complicated data structures and may thereby relieve the characterization phase in classical proteomics. Traditionally statistical methods are not suitable for analysis of the huge amounts of data, where the number of variables exceed the number of objects. Multivariate data analysis, on the other hand, may uncover the hidden structures present in these data. This study takes its starting point in the field of classical proteomics and shows how multivariate data analysis can lead to faster ways of finding interesting proteins. Multivariate analysis has shown interesting results as a supplement to classical proteomics and added a new dimension to the field of proteomics.






Gottlieb DM,Schultz J,Bruun SW,Jacobsen S,Søndergaard I




Has Abstract


2004-06-01 00:00:00














  • Putrescine N-methyltransferase--the start for alkaloids.

    abstract::Putrescine N-methyltransferase (PMT) catalyses S-adenosylmethionine (SAM) dependent methylation of the diamine putrescine. The product N-methylputrescine is the first specific metabolite on the route to nicotine, tropane, and nortropane alkaloids. PMT cDNA sequences were cloned from tobacco species and other Solanacea...


    pub_type: 杂志文章,评审


    authors: Biastoff S,Brandt W,Dräger B

    更新日期:2009-10-01 00:00:00

  • The biosynthesis of monolignols: a "metabolic grid", or independent pathways to guaiacyl and syringyl units?

    abstract::Lignin is a complex polymer formed by the oxidative polymerization of hydroxycinnamyl alcohol derivatives termed monolignols. The major monolignols in dicotyledonous angiosperm lignin are monomethylated guaiacyl (G) units derived from coniferyl alcohol, and dimethylated syringyl (S) units derived from sinapyl alcohol....


    pub_type: 杂志文章,评审


    authors: Dixon RA,Chen F,Guo D,Parvathi K

    更新日期:2001-08-01 00:00:00

  • Five labdane diterpenoids from the seeds of Aframomum zambesiacum.

    abstract::Five labdane diterpenoids, (3-5), zambesiacolactone A (7) and zambesiacolactone B (8), were isolated from the seeds of Aframomum zambesiacum (Baker) K. Schum., along with five known labdanes and a linear sesquiterpene, nerolidol. Their structures were elucidated by spectroscopic analysis. Their antiplasmodial activity...


    pub_type: 杂志文章


    authors: Kenmogne M,Prost E,Harakat D,Jacquier MJ,Frédérich M,Sondengam LB,Zèches M,Waffo-Téguo P

    更新日期:2006-03-01 00:00:00

  • An anti-staphylococcal acylphloroglucinol from Hypericum foliosum.

    abstract::An investigation into the antibacterial properties of Hypericum foliosum Aiton. (Guttiferae) has led to the isolation of a new bioactive acylphloroglucinol natural product which by NMR spectroscopy and mass spectrometry was characterised as 1,3,5-trihydroxy-6-[2''',3'''-epoxy-3'''-methyl-butyl]-2-[2''-methyl-butanoyl]...


    pub_type: 杂志文章


    authors: Gibbons S,Moser E,Hausmann S,Stavri M,Smith E,Clennett C

    更新日期:2005-06-01 00:00:00

  • Chemical constituents of two Mollinedia species.

    abstract::A butanolide, marliolide, was isolated from the hexane extract of leaves of Mollinedia marliae (Monimiacae) along with the long chain fatty alcohol hexacosanol. Phytol and a mixture of sitosterol and stigmasterol were also isolated from leaves of both M. marliae and M. gilgiana. trans-N-Feruloyltyramine, a ferulic aci...


    pub_type: 杂志文章


    authors: Claros BM,da Silva AJ,Vasconcellos ML,de Brito AP,Leitão GG

    更新日期:2000-12-01 00:00:00

  • Transformations of testosterone and related steroids by Botrytis cinerea.

    abstract::Botrytis cinerea (strain AM235) was used to investigate the transformations of testosterone and related steroids. It was found that the position and stereochemistry of the introduced hydroxyl group, as well as the yield of products, depended on the structure of the substrate. Botrytis cinerea converts the examined sub...


    pub_type: 杂志文章


    authors: Huszcza E,Dmochowska-Gładysz J

    更新日期:2003-01-01 00:00:00

  • Biosynthesis of the hyperforin skeleton in Hypericum calycinum cell cultures.

    abstract::Hyperforin is an important antidepressant constituent of Hypericum perforatum (St. John's wort). Cell cultures of the related species H. calycinum were found to contain the homologue adhyperforin and to a low extent hyperforin, when grown in BDS medium in the dark. Adhyperforin formation paralleled cell culture growth...


    pub_type: 杂志文章


    authors: Klingauf P,Beuerle T,Mellenthin A,El-Moghazy SA,Boubakir Z,Beerhues L

    更新日期:2005-01-01 00:00:00

  • 4alpha-methyl-24beta-ethyl-5alpha-cholesta-14,25-dien-3beta-ol and 24beta-ethylcholesta-5, 9(11), 22E-trien-3beta-ol, sterols from Clerodendrum inerme.

    abstract::From the aerial parts of Clerodendrum inerme, two new sterols (4alpha-methyl-24beta-ethyl-5alpha-cholesta-14, 25-dien-3beta-ol and 24beta-ethylcholesta-5, 9(11), 22E-trien-3beta-ol) and a new aliphatic ketone (11-pentacosanone) were isolated together with another known aliphatic ketone (6-nonacosanone) and a diterpene...


    pub_type: 杂志文章


    authors: Pandey R,Verma RK,Singh SC,Gupta MM

    更新日期:2003-06-01 00:00:00

  • Purification and partial biochemical characterization of polyphenol oxidase from mamey (Pouteria sapota).

    abstract::While a long shelf life for fruit products is highly desired, enzymatic browning is the main cause of quality loss in fruits and is therefore a main problem for the food industry. In this study polyphenol oxidase (PPO), the main enzyme responsible for browning was isolated from mamey fruit (Pouteria sapota) and charac...


    pub_type: 杂志文章


    authors: Palma-Orozco G,Ortiz-Moreno A,Dorantes-Alvarez L,Sampedro JG,Nájera H

    更新日期:2011-01-01 00:00:00

  • Anthocyanin determination in blueberry extracts from various cultivars and their antiproliferative and apoptotic properties in B16-F10 metastatic murine melanoma cells.

    abstract::Blueberry consumption is associated with health benefits contributing to a reduced risk for cardiovascular disease, diabetes and cancer. The aim of this study was to determine the anthocyanin profile of blueberry extracts and to evaluate their effects on B16-F10 metastatic melanoma murine cells. Seven blueberry cultiv...


    pub_type: 杂志文章


    authors: Bunea A,Rugină D,Sconţa Z,Pop RM,Pintea A,Socaciu C,Tăbăran F,Grootaert C,Struijs K,VanCamp J

    更新日期:2013-11-01 00:00:00

  • Identification and functional expression of a type 2 acyl-CoA:diacylglycerol acyltransferase (DGAT2) in developing castor bean seeds which has high homology to the major triglyceride biosynthetic enzyme of fungi and animals.

    abstract::Seed oil from castor bean (Ricinus communis) contains high amounts of hydroxy fatty acid rich triacylglycerols (TAGs) that can serve as raw material for production of bio-based products such as nylon, cosmetics, lubricants, foams, and surfactants. Diacylglycerol acyltransferase (DGAT) catalyses the terminal reaction i...


    pub_type: 杂志文章


    authors: Kroon JT,Wei W,Simon WJ,Slabas AR

    更新日期:2006-12-01 00:00:00

  • alpha-Glucosidase inhibitors from the seeds of Syagrus romanzoffiana.

    abstract::Bioassay-guided fractionation against alpha-glucosidase resulted in isolation and characterization of eight active compounds from the EtOH extract of the seeds of Syagrus romanzoffiana. Of these, seven are stilbenoids, and two of them, 13-hydroxykompasinol A (1) and scirpusin C (4), possess potent inhibitory activity ...


    pub_type: 杂志文章


    authors: Lam SH,Chen JM,Kang CJ,Chen CH,Lee SS

    更新日期:2008-03-01 00:00:00

  • Cadinane sesquiterpenoids from the basidiomycete Stereum cf. sanguinolentum BCC 22926.

    abstract::Stereumins Q-U, together with known stereumins A, B, K, L, and N, as well as ent-strobilols E and G were isolated from the culture of Stereum cf. sanguinolentum BCC 22926. Their structures were elucidated by extensive spectroscopic analyses. The absolute configurations of stereumins A and Q, as well as ent-strobilol E...


    pub_type: 杂志文章


    authors: Bunyapaiboonsri T,Yoiprommarat S,Nopgason R,Komwijit S,Veeranondha S,Puyngain P,Boonpratuang T

    更新日期:2014-09-01 00:00:00

  • Mangiterpenes A-C and 2',3'-seco-manginoid C, four sesquiterpene/monoterpene-shikimate-conjugated spirocyclic meroterpenoids from Guignardia mangiferae.

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    pub_type: 杂志文章


    authors: Chen K,Chen C,Guo J,Sun W,Liu J,Yang J,Liu X,Wang J,Luo Z,Zhu H,Zhang Y

    更新日期:2019-08-01 00:00:00

  • Intermediates in the oxidative pathway from torulene to torularhodin in the red yeasts Cystofilobasidium infirmominiatum and C. capitatum (Heterobasidiomycetes, Fungi).

    abstract::Two red Cystofilobasidium spp. isolated from spring sap-flows of Betula pendula were analysed for their carotenoid content. In Cystofilobasidium infirmominiatum, three unusual pigments were detected and identified by structure elucidation as oxidised torulene derivatives. These included 16'-hydroxytorulene and torular...


    pub_type: 杂志文章


    authors: Herz S,Weber RW,Anke H,Mucci A,Davoli P

    更新日期:2007-10-01 00:00:00

  • Cardiac glycosides from Yellow Oleander (Thevetia peruviana) seeds.

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    pub_type: 杂志文章


    authors: Kohls S,Scholz-Böttcher BM,Teske J,Zark P,Rullkötter J

    更新日期:2012-03-01 00:00:00

  • Pithedulosides A-G, oleanane glycosides from Pithecellobium dulce.

    abstract::Seven saponins named pithedulosides A-G were isolated from the seeds of Pithecellobium dulce. Their structures were established through spectral analyses as echinocystic acid 3-O-alpha-L-arabinopyranosyl-(1-->6)-beta-D-glucopyranoside, echinocystic acid and oleanolic acid 3-O-alpha-L-arabinopyranosyl- (1-->2)-alpha-L-...


    pub_type: 杂志文章


    authors: Nigam SK,Gopal M,Uddin R,Yoshikawa K,Kawamoto M,Arihara S

    更新日期:1997-04-01 00:00:00

  • Triumphalone, a diketone from the volatile oil of the leaves of Melaleuca triumphalis, and its spontaneous conversion into isotriumphalone.

    abstract::The major component (35-65%) of the volatile oil obtained by steam distillation of the leaves of Melaleuca triumphalis has been identified as (rel)-1beta-pentyl-1alpha,6alpha-dihydroxy-3,3,5,5-tetramethylcyclohexa-2,4-dione (trivial name triumphalone). Relative stereochemistry was established by nuclear Overhauser exp...


    pub_type: 杂志文章


    authors: Brophy JJ,Craig DC,Goldsack RJ,Fookes CJ,Leach DN,Waterman PG

    更新日期:2006-09-01 00:00:00

  • Flavonoids as chemotaxonomic markers in the genus Drosera.

    abstract::The botanical classification of the huge genus Drosera remains controversial since long. In the present study, the pattern of major phenolic compounds in ten Drosera species belonging to seven different subgenera and/or sections of the genus was investigated for chemotaxonomic allocation. The composition of flavonoids...


    pub_type: 杂志文章


    authors: Braunberger C,Zehl M,Conrad J,Wawrosch C,Strohbach J,Beifuss U,Krenn L

    更新日期:2015-10-01 00:00:00

  • Pregnane glycosides from fruits of Balanites aegyptiaca.

    abstract::From the mesocarp of Balanites aegyptiaca fruits, two pregnane glycosides were isolated. One is new and identified as pregn-5-ene-3 beta,16 beta,20(R)-triol 3-O-(2,6-di-O-alpha-L-rhamnopyranosyl)-beta-D-glucopyranoside (balagyptin), while the other is known and assigned as pregn-5-ene-3 beta,16 beta,20(R)-triol 3-O-be...


    pub_type: 杂志文章


    authors: Kamel MS,Koskinen A

    更新日期:1995-12-01 00:00:00

  • Biosynthesis of cyanohydrin glucosides from unnatural nitriles in intact tissue of Passiflora morifolia and Turnera angustifolia.

    abstract::Passiflora morifolia, which under natural conditions contains cyanohydrin glucosides linamarin, lotaustralin and epilotaustralin, converted cyclopentanecarbonitrile, 2-cyclopentenecarbonitrile and 3-methylbutanenitrile into the corresponding cyanohydrin glucosides. Turnera angustifolia, which normally produces glucosi...


    pub_type: 杂志文章


    authors: Jaroszewski JW,Rasmussen AB,Rasmussen HB,Olsen CE,Jørgensen LB

    更新日期:1996-06-01 00:00:00

  • Sesquiterpenes from Warburgia ugandensis and their antimycobacterial activity.

    abstract::The dichloromethane extract of the stem bark of Warburgia ugandensis afforded three new coloratane sesquiterpenes, namely: 6alpha,9alpha-dihydroxy-4(13),7-coloratadien-11,12-dial (1), 4(13),7-coloratadien-12,11-olide (2), and 7beta-hydroxy-4(13),8-coloratadien-11,12-olide (3), together with nine known sesquiterpenes, ...


    pub_type: 杂志文章


    authors: Wube AA,Bucar F,Gibbons S,Asres K

    更新日期:2005-10-01 00:00:00

  • A seco-laurane sesquiterpene and related laurane derivatives from the red alga Laurencia okamurai Yamada.

    abstract::A ring-cleaved sesquiterpene, named seco-laurokamurone, four laurane-type sesquiterpenes, laurepoxyene, 3β-hydroperoxyaplysin, 3α-hydroperoxy-3-epiaplysin, and 8,10-dibromoisoaplysin, one laurokamurane-type sesquiterpene, laurokamurene D, and one bisabolane-type sesquiterpene, (5S)-5-acetoxy-β-bisabolene, have been is...


    pub_type: 杂志文章


    authors: Yu XQ,He WF,Liu DQ,Feng MT,Fang Y,Wang B,Feng LH,Guo YW,Mao SC

    更新日期:2014-07-01 00:00:00

  • Glutathione reductase activity and isoforms in leaves and roots of wheat plants subjected to cadmium stress.

    abstract::The behavior of glutathione reductase (GR, EC activity and isoforms were analyzed in wheat (Triticum aestivum L.) leaves and roots exposed to a chronic treatment with a toxic cadmium (Cd) concentration. A significant growth inhibition (up to 55%) was found in leaves at 7, 14 and 21 days, whereas roots were af...


    pub_type: 杂志文章


    authors: Yannarelli GG,Fernández-Alvarez AJ,Santa-Cruz DM,Tomaro ML

    更新日期:2007-02-01 00:00:00

  • Dietary phytoestrogens and health.

    abstract::The interest in the potential health effects of dietary phytoestrogens has increased with the findings that hormone replacement therapy is not as safe or effective as previously thought. This review summarizes the dietary sources of the phytoestrogens; isoflavonoids, stilbenes, coumestans and lignans. It also examines...


    pub_type: 杂志文章,评审


    authors: Cornwell T,Cohick W,Raskin I

    更新日期:2004-04-01 00:00:00

  • Chondroprotective and anti-inflammatory effects of sesamin.

    abstract::Osteoarthritis (OA) is a major disability of elderly people. Sesamin is the main compound in Sesamun indicum Linn., and it has an anti-inflammatory effect by specifically inhibiting Δ5-desaturase in polyunsaturated fatty acid biosynthesis. The chondroprotective effects of sesamin were thus studied in a porcine cartila...


    pub_type: 杂志文章


    authors: Phitak T,Pothacharoen P,Settakorn J,Poompimol W,Caterson B,Kongtawelert P

    更新日期:2012-08-01 00:00:00

  • Unusual cyclolanostanes from leaves of Pandanus boninensis.

    abstract::Two unusual triterpenoids, (24S)-24-methyl-25,32-cyclo-5alpha-lanosta-9(11)-en-3beta-ol and (24S)-24-methyl-25,32-cyclo-cycloartane-3beta-ol, were isolated from leaves of Pandanus boninensis along with known triterpenoids and lignans. Their structures were established on the basis of spectroscopic methods and X-ray an...


    pub_type: 杂志文章


    authors: Inada A,Ikeda Y,Murata H,Inatomi Y,Nakanishi T,Bhattacharyya K,Kar T,Bocelli G,Cantoni A

    更新日期:2005-12-01 00:00:00

  • Biosynthesis of unusual monocyclic alkenes by the diatom Rhizosolenia setigera (Brightwell).

    abstract::Novel, polyunsaturated monocyclic sester- and triterpenes isolated from the diatom Rhizosolenia setigera (Brightwell), are biosynthesised mainly via the mevalonate pathway. The experiments involved incubation of the alga with [1-(13)C]acetate, isolation of the alkenes by extraction and silver ion HPLC, followed by det...


    pub_type: 杂志文章


    authors: Massé G,Belt ST,Rowland SJ

    更新日期:2004-04-01 00:00:00

  • Chrysin and other leaf exudate flavonoids in the genus Pelargonium.

    abstract::In a chemotaxonomic survey of 57 Pelargonium species, leaf exudate flavonoids were detected in 35% of the sample, mostly in trace amounts. However, chrysin and a related C-methylflavanone were identified as major leaf surface constituents of P. crispum, and a mixture of quercetin and kaempferol mono-, and di- and trim...


    pub_type: 杂志文章


    authors: Williams CA,Harborne JB,Newman M,Greenham J,Eagles J

    更新日期:1997-12-01 00:00:00

  • Polygalasaponins XLII-XLVI from roots of Polygala glomerata.

    abstract::Five new oleanane-type saponins, polygalasaponins XLII-XLVI, along with two known saponins were isolated from the roots of Polygala glomerata Lour. The structures of polygalasaponins XLII-XLVI were elucidated as 3-O-beta-D-glucopyranosyl presenegenin 28-O-beta-D-xylopyranosyl-(1-->4)-alpha-L-rhamnopyranosyl- (1-->2)-{...


    pub_type: 杂志文章


    authors: Zhang D,Miyase T,Kuroyanagi M,Umehara K,Noguchi H

    更新日期:1998-02-01 00:00:00