A 2-oxoglutarate-dependent dioxygenase is integrated in DIMBOA-biosynthesis.

Abstract:

:Benzoxazinoids are secondary metabolites of grasses that function as natural pesticides. While many steps of DIMBOA biosynthesis have been elucidated, the mechanism of the introduction of OCH(3)-group at the C-7 position was unknown. Inhibitor experiments in Triticum aestivum and Zea mays suggest that a 2-oxoglutarate-dependent dioxygenase catalyses the hydroxylation reaction at C-7. Cloning and reverse genetics analysis have identified the Bx6 gene that encodes this enzyme. Bx6 is located in the Bx-gene cluster of maize.

journal_name

Phytochemistry

journal_title

Phytochemistry

authors

Frey M,Huber K,Park WJ,Sicker D,Lindberg P,Meeley RB,Simmons CR,Yalpani N,Gierl A

doi

10.1016/s0031-9422(02)00556-3

subject

Has Abstract

pub_date

2003-02-01 00:00:00

pages

371-6

issue

3

eissn

0031-9422

issn

1873-3700

pii

S0031942202005563

journal_volume

62

pub_type

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