Vacuolar uptake of the phytoalexin medicarpin by the glutathione conjugate pump.

Abstract:

:We have studied the uptake of [3H]-medicarpin and its glutathione conjugate(s) into vacuolar membrane vesicles from etiolated hypocotyls of mung bean (Vigna radiata). Unconjugated medicarpin is taken up at a low rate in the presence or absence of MgATP. However, [3H]-medicarpin-glutathione conjugate(s), prepared by incubation of medicarpin with a total maize glutathione S-transferase preparation, is taken up more than four-fold faster than medicarpin in the presence of MgATP, and this uptake is MgATP-dependent. Uptake of medicarpin-glutathione was not significantly inhibited by the ionophore gramicidin-D, but was strongly inhibited by vanadate and the alternative transport substrate S-(2,4-dinitrophenyl) glutathione. Our results demonstrate, in a model system, the potential utilization of the high affinity, high capacity, uncoupler-insensitive glutathione conjugate pump for the vacuolar transport of an isoflavonoid phytoalexin.

journal_name

Phytochemistry

journal_title

Phytochemistry

authors

Li ZS,Alfenito M,Rea PA,Walbot V,Dixon RA

doi

10.1016/s0031-9422(97)00031-9

subject

Has Abstract

pub_date

1997-06-01 00:00:00

pages

689-93

issue

4

eissn

0031-9422

issn

1873-3700

pii

S0031-9422(97)00031-9

journal_volume

45

pub_type

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