Abstract:
:We have studied the uptake of [3H]-medicarpin and its glutathione conjugate(s) into vacuolar membrane vesicles from etiolated hypocotyls of mung bean (Vigna radiata). Unconjugated medicarpin is taken up at a low rate in the presence or absence of MgATP. However, [3H]-medicarpin-glutathione conjugate(s), prepared by incubation of medicarpin with a total maize glutathione S-transferase preparation, is taken up more than four-fold faster than medicarpin in the presence of MgATP, and this uptake is MgATP-dependent. Uptake of medicarpin-glutathione was not significantly inhibited by the ionophore gramicidin-D, but was strongly inhibited by vanadate and the alternative transport substrate S-(2,4-dinitrophenyl) glutathione. Our results demonstrate, in a model system, the potential utilization of the high affinity, high capacity, uncoupler-insensitive glutathione conjugate pump for the vacuolar transport of an isoflavonoid phytoalexin.
journal_name
Phytochemistryjournal_title
Phytochemistryauthors
Li ZS,Alfenito M,Rea PA,Walbot V,Dixon RAdoi
10.1016/s0031-9422(97)00031-9subject
Has Abstractpub_date
1997-06-01 00:00:00pages
689-93issue
4eissn
0031-9422issn
1873-3700pii
S0031-9422(97)00031-9journal_volume
45pub_type
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