Abstract:
:We developed the Auto Cyto Fix (ACF) 1000 as an experimental model, which was adapted as a membrane filter method for automatically making smear preparations fixed with 95% ethanol. In this study, immunohistochemistry was applied to the cells smeared on the membrane filter, and effectiveness was examined. Twenty-four effusions and HeLa 229 cells were used. These cell samples were produced by the ACF1000 on the membrane filter and stained by the Papanicolaou method. After observation, these were decolored and stained by immunohistochemical techniques. Antibodies included CEA, EMA, and MIB-1. CEA and EMA were detected by the indirect method, and were colored by DAB, AEC, and new fuchsin. MIB-1 was detected by the immunoperoxidase (LSAB) and the immunofluorescence method. A uniformly stabilized immunoreaction was obtained and was equivalent to or better than that of the conventional technique. In addition, there was no background staining on the membrane filter. Based on these results, the membrane filter preparation produced by the ACF1000 was also effectively applied to immunohistochemical observations.
journal_name
Diagn Cytopatholjournal_title
Diagnostic cytopathologyauthors
Iijima J,Shiina Y,Ohkoudo M,Sakuma Kdoi
10.1002/dc.10041subject
Has Abstractpub_date
2002-01-01 00:00:00pages
56-60issue
1eissn
8755-1039issn
1097-0339pii
10.1002/dc.10041journal_volume
26pub_type
杂志文章abstract::Cytohistologic correlation was performed by 3 observers on 100 atypical squamous cells of undetermined significance (ASCUS) cases from a colposcopy clinic. Our objectives were to: 1) subclassify ASCUS cases and determine their clinical significance; 2) assess the independent predictive value of different cytologic par...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199909)21:3<211::aid-dc14>
更新日期:1999-09-01 00:00:00
abstract::Fine-needle aspiration (FNA) is a minimally invasive, fast, and accurate diagnostic method for the evaluation of patients with locally recurrent or distant metastases of malignant melanoma. In the vast majority of cases, the diagnosis is straightforward with the characteristic cytologic features well documented in the...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20952
更新日期:2009-01-01 00:00:00
abstract::Juvenile xanthogranuloma (JXG) is a rare type of non-Langerhan cell histiocytic disorder, which is mostly confined to skin of head and neck. It is a self-limiting benign condition, which does not require surgery. We present a case of 8-month-old girl child with multiple yellowish brown colored papules over scalp, face...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24310
更新日期:2020-01-01 00:00:00
abstract::Nipple discharge (ND) either in the presence or in the absence of underlying mass is a very uncommon presentation in males and its bloody nature is still rarer. ND cytology in males has not been reported much in the literature. Spontaneous and expressed ND, however, can be very useful in establishing the diagnosis. Tw...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24080
更新日期:2019-02-01 00:00:00
abstract::Early and accurate diagnosis of hepatocellular carcinoma in liver nodules using fine-needle aspiration biopsy can still pose difficulties, particularly in cirrhotic nodules containing dysplastic hepatocytes. Loss of architectural clues, which may aid diagnosis, can be overcome by using confocal scanning laser microsco...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/1097-0339(200008)23:2<82::aid-dc3>3.0.co;2
更新日期:2000-08-01 00:00:00
abstract::We report a very unusual cytologic feature, intranuclear inclusion bodies, in mesothelioma of a predominantly adenomatoid type. The patient, a 57-year-old woman, was presented with dyspnea and right pleural effusion. Pleural aspiration cytology revealed many cohesive ball-like clusters, with a tubular pattern, compose...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.22932
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND:The 2012 American Society for Colposcopy and Cervical Pathology Consensus Guidelines provide information for managing abnormal cervical cancer screening tests and cancer precursors. According to these guidelines for Pap smear diagnosis of Atypical squamous cells of undetermined significance, reflex high risk...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24158
更新日期:2019-07-01 00:00:00
abstract::Breast disease is of great concern to patients and health care providers alike. Malignancies of the breast and their various presentations can pose tremendous challenges with respect to early diagnosis and effective treatment. Carcinoma of the breast may appear in several forms and produce one or more ectopic substanc...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.20485
更新日期:2006-10-01 00:00:00
abstract::Herpes simplex is an uncommon cause of lower respiratory tract infection that requires prompt diagnosis and treatment to prevent late complications. We report two cases with simultaneous herpes simplex virus infection of the lower respiratory tract and lung carcinoma. Cytology of bronchial brushing and washing fluids ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20921
更新日期:2008-11-01 00:00:00
abstract::Although subacute granulomatous thyroiditis (SGT) is usually diagnose clinically, there are other thyroid conditions that must be ruled out. This task is achieved by means of fine-needle aspiration (FNA). In retrospect, the clinical and cytologic findings seen in 36 SGT cases are reassessed with a view to deciding whi...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199703)16:3<214::aid-dc4>3
更新日期:1997-03-01 00:00:00
abstract::E-cadherin (E-CD) is a cell-adhesion molecule that has been associated with invasion and metastasis in a wide variety of human neoplasms. We have recently shown that, although decreased E-CD expression is associated with increased bladder-wall invasion and higher tumor grade of infiltrating transitional cell carcinoma...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0339(199605)14:4<310::AID-DC6>3
更新日期:1996-06-01 00:00:00
abstract:BACKGROUND:Cytology of the adrenal gland is a less performed technique even in tertiary care centres; yet cytological evaluation is an important diagnostic tool for assessment of adrenal lesions. Our objective was to evaluate the diagnostic utility of FNAC smears and cellblock with immunohistochemistry (IHC) in lesions...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24484
更新日期:2020-05-23 00:00:00
abstract::We report the fine-needle aspiration cytology of a case of metastatic small cell osteosarcoma to the liver with rosette formation, originating from distal femur, in a 36-year-old female. The aspirate of the liver metastases revealed a relatively monomorphic population of mitotically active small blue round cells arran...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.22894
更新日期:2014-02-01 00:00:00
abstract::A case of histologically and immunocytochemically confirmed pemphigus vulgaris of the nipple examined by scrape cytology is reported. The scrape cytology showed isolated and somewhat loosely clustered cells with basophilic cytoplasm and vesicular hyperchromatic nuclei dysplaying prominent nucleoli, suggesting Paget's ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199702)16:2<156::aid-dc13>
更新日期:1997-02-01 00:00:00
abstract::Cytological findings are presented of seven cases of cardiac myxomas. Avidin-biotin-peroxidase complex (ABC) method was employed to demonstrate Ulex europaeus agglutinin-I (UEA-I) lectin in imprint smears as well as in paraffin-embedded tissue sections in cardiac myxomas. The cytology was characterized by tumor cells ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840090611
更新日期:1993-12-01 00:00:00
abstract::Adenoid cystic carcinoma (ACC) occurs not only as a tumor of salivary glands, but also in very unusual locations, such as in the skin. Only very few cases of primary cutaneous of ACC have metastasized to the lymph nodes and lungs. We present a 53-year-old man with metastasis of the pericardium from a primary cutaneous...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20818
更新日期:2008-05-01 00:00:00
abstract::We evaluated the diagnostic contribution of adjunct studies performed on aspirated material in the work-up of pediatric fine-needle aspiration (FNA) biopsies. Ancillary studies were performed on 54 of 136 (39.7%) pediatric FNA biopsies during a 5-year period. In 23 (16.9%) cases, immunocytochemical (ICC) studies, cons...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840080210
更新日期:1992-01-01 00:00:00
abstract::Human papillomavirus (HPV)-related head and neck squamous cell carcinoma (HNSCC) is a unique form of carcinoma that largely arises from the tonsillar tissue in the oropharynx. These tumors often present with cervical lymphadenopathy resulting in a fine needle aspiration (FNA) biopsy. Use of the cytology specimen to de...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.23668
更新日期:2017-03-01 00:00:00
abstract::Metastatic malignancy represents a common cause of effusions. Immunocytochemistry (ICC) is useful in confirming malignancy and gaining insight into the site of origin. Cell blocks are commonly utilized for this purpose; nonetheless, when the malignant cells are sparse, they may not be represented in cell blocks thereb...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.22852
更新日期:2013-05-01 00:00:00
abstract::Most granulomas of the prostate are nonspecific; infectious, post-operative, and allergic lesions are much less common. Fine-needle aspiration findings in the typical case are distinctive and easily recognized. Several series have been reported, but few have included histologic follow-up. We describe three cases of gr...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840070513
更新日期:1991-01-01 00:00:00
abstract::The biological behavior of neuroendocrine tumors (NETs) is heterogeneous and differs from that of cholangiocarcinoma, which is the most common malignant tumor of the biliary tree. However, the preoperative diagnosis of NET in the biliary tree is extremely difficult and to our knowledge, diagnosis by brush cytology has...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24173
更新日期:2019-07-01 00:00:00
abstract::Pleomorphic lipoma is a rare soft-tissue tumor, most commonly seen in the head and neck regions of middle-aged men. Fine-needle aspiration (FNA) of these lesions can present a diagnostic challenge. Its large, hyperchromatic cells and multinucleated forms (floret cells) can easily be mistaken for a malignancy. A patien...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20183
更新日期:2005-02-01 00:00:00
abstract:BACKGROUND:High-risk Human papillomavirus (HR-HPV)-associated head and neck squamous cell carcinoma (HNSCC) is a distinct epidemiologic and pathologic disease. The data of HR-HPV testing on fine-needle aspiration (FNA) materials of cervical lymph nodes in patients with metastatic HNSCC are limited. METHODS:We retrospe...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23548
更新日期:2016-10-01 00:00:00
abstract::Over an 8-yr period at the Houston Veterans Hospital, 731 endoscopic gastric salvage cytologies were performed in 671 patients, with surgical biopsy also done in 660. Seventy-six patients subsequently had tissue biopsy-proven or resection-proven malignancies. Salvage cytology had a sensitivity of 91% and a specificity...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840060102
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:Liquid-based cytology (LBC) testing induces morphologic changes due to the use of specific fixatives and preparation techniques, and the cytologies of effusions determined in this manner differ morphologically from those of conventional cytopreparation (CCP) smear methods. We compared the cytologic features ...
journal_title:Diagnostic cytopathology
pub_type: 临床试验,杂志文章
doi:10.1002/dc.24365
更新日期:2020-03-01 00:00:00
abstract::Synovial sarcoma (SS) is one of the most common soft tissue tumors that typically presents in the extremities of young adults, but may occur at any site and affect children during the first decade. Herein we discuss a 12-yr-old male who complained of left foot pain and plantar mass. A fine-needle aspiration biopsy of ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20566
更新日期:2006-11-01 00:00:00
abstract:BACKGROUND:Diagnosis of hepatoblastoma (HBL) is based on characteristic clinical and radiological presentation, young age and marked elevation of serum α-fetoprotein (aFP). Fine needle aspiration (FNA) technique is successfully used in the diagnosis of hepatoblastoma. To evaluate the value of FNA in the diagnosis and s...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23642
更新日期:2017-02-01 00:00:00
abstract::We report a rare case of extranodal NK/T-cell lymphoma, nasal type of the uterine cervix that showed cytologic features mimicking cervical cancer. A 65-year-old woman presented with vaginal bleeding. Gynecological examination revealed a bulky tumor of the cervix. A conventional Papanicolaou-stained cervical smear show...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23439
更新日期:2016-05-01 00:00:00
abstract::A 30-year-old female presented with a three-month history of a multilocular cystic lesion over the lumbosacral spine. Fine-needle aspiration biopsy (FNA) of the lesion was performed at an outside institution, and a cytologic diagnosis, suspicious for chordoma, was rendered. The patient presented for surgical consultat...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23871
更新日期:2018-05-01 00:00:00
abstract::Fine-needle aspiration cytology (FNAC) is a technique that can overcome tissue-sampling disaggregation problems related to DNA flow cytometry analysis. The aim of this study, with long-term follow-up (median, 72 mo), was to investigate the prognostic value of DNA ploidy and S-phase fraction (SPF) in patients with non-...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.10298
更新日期:2003-10-01 00:00:00