Analysis of three-dimensional architecture in Feulgen-stained fine-needle liver aspirates, using confocal scanning laser microscopy.

Abstract:

:Early and accurate diagnosis of hepatocellular carcinoma in liver nodules using fine-needle aspiration biopsy can still pose difficulties, particularly in cirrhotic nodules containing dysplastic hepatocytes. Loss of architectural clues, which may aid diagnosis, can be overcome by using confocal scanning laser microscopic examination of tissue fragments in smears without the need for further processing of slides. The basic fuchsin of the Feulgen method resulted in excellent confocal images without the need for further processing of the smears. Clear morphological differences in three-dimensional reconstructions of optically sectioned tissue fragments were demonstrated in normal, hyperplastic, and malignant smears as an aid to diagnosis.

journal_name

Diagn Cytopathol

journal_title

Diagnostic cytopathology

authors

Boon ME,Barlow Y,Marres EM,Zeppa P,Kok LP

doi

10.1002/1097-0339(200008)23:2<82::aid-dc3>3.0.co;2

subject

Has Abstract

pub_date

2000-08-01 00:00:00

pages

82-6

issue

2

eissn

8755-1039

issn

1097-0339

pii

10.1002/1097-0339(200008)23:2<82::AID-DC3>3.0.CO;2

journal_volume

23

pub_type

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