Abstract:
:We applied TdT-mediated deoxyuridine triphosphate (dUTP)-biotin nick end labeling (TUNEL) to cytologic smears in order to detect the cells undergoing apoptosis. These smears were obtained by scraping the cut surface of 9 cases of carcinoma, including renal-cell carcinoma (3 cases), esophageal squamous-cell carcinoma (3 cases), and gastric adenocarcinoma (3 cases), and were fixed and prepared by different methods. The results were also compared with those of tissue sections. TUNEL in smears was generally associated with higher background nuclear stain than in tissue sections. Smears that were fixed in 4% or 8% paraformaldehyde or absolute methanol exhibited results comparable with those of tissue sections, with minimum background in all cases examined. There were no significant differences in TUNEL labeling index among tissue sections and smears fixed in 4% or 8% paraformaldehyde or in absolute methanol. Smears treated in Carnoy's fixative (3:1 methanol:acetic acid) and air-dried smears demonstrated a higher background. TUNEL positivity could not be detected in slides decolorized from May-Grünwald-Giemsa stain. Markedly high background, which may occur as a result of artifactural DNA breaks, was also observed in slides decolorized from Papanicolaou stain, in which TUNEL-positive cells could be evaluated only in 3/8 cases. Application of the TUNEL method to cytology specimens has disadvantages or limitations compared to its application to histological sections, but the method is considered the most suitable one for detecting cells undergoing apoptosis in cytology materials.
journal_name
Diagn Cytopatholjournal_title
Diagnostic cytopathologyauthors
Sasano H,Yamaki H,Nagura Hdoi
10.1002/(sici)1097-0339(199806)18:6<398::aid-dc3>3subject
Has Abstractpub_date
1998-06-01 00:00:00pages
398-402issue
6eissn
8755-1039issn
1097-0339pii
10.1002/(SICI)1097-0339(199806)18:6<398::AID-DC3>3journal_volume
18pub_type
杂志文章abstract::A silver staining technique was applied to 51 thyroid smears. The numbers of silver-stained nucleolar organizer regions (AgNORs) were counted, and the mean AgNOR and nuclear area per cell were determined with an image analyzer. The mean AgNOR count per cell was significantly higher in malignant than in benign lesions,...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0339(199603)14:2<140::AID-DC7>3
更新日期:1996-03-01 00:00:00
abstract::Cell blocks (CBs) are often prepared with fine-needle aspirates (FNAs) from multiple organs as an adjunct to smears in the diagnosis of aspirated lesions. However, the literature contains few reports on their utility with regard to specific organ sites. At our institution, CBs are made routinely on FNAs when there is ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20385
更新日期:2006-02-01 00:00:00
abstract::Gout, a disease resulting from the effects of hyperuricemia and a crystal-induced arthropathy, may produce soft tissue masses (tophi) which mimick neoplasia clinically and radiographically. We have recently diagnosed three cases of gouty tophus, two of which were clinically suspected to represent sarcomas, by fine-nee...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199707)17:1<30::aid-dc6>3.
更新日期:1997-07-01 00:00:00
abstract::True malignant mixed tumor (carcinosarcoma) of salivary gland is a very rare neoplasm. Only one brief account of the Fine-Needle Aspiration Biopsy (FNAB) cytologic findings of this tumor was found in the literature. The authors report a case of malignant mixed tumor (chondrosarcoma and epidermoid carcinoma) arising fr...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840070419
更新日期:1991-01-01 00:00:00
abstract::Myoepithelial cells are an important component of salivary gland tumors and are partly responsible from the diverse histology of them. In this study, we focus on the myoepithelial cell differentiation by using cytological morphology in a various types of salivary gland tumors especially with regard to their contributi...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21544
更新日期:2012-03-01 00:00:00
abstract:BACKGROUND:The International Academy of Cytology (IAC) Yokohama system is a recently proposed system for reporting breast cytology by fine needle aspiration biopsies (FNAB). Multiple studies are needed to confirm the risk of malignancy (ROM) of the various reporting categories of this system. The present article studie...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24632
更新日期:2021-02-01 00:00:00
abstract::Diffuse large B-cell lymphoma (DLBCL) with fibrillary matrix is an extremely rare variant of non-Hodgkin's lymphoma (NHL) whose pathological features are poorly known. Here, we report on our experience with such a condition arising in a 71-yr-old woman. Our findings not only demonstrate that DLBCL with fibrillary matr...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.10172
更新日期:2002-10-01 00:00:00
abstract::Despite various reports of BK viral (BKV) DNA sequences or proteins in tumors of the urogenital tract, there has been no study statistically linking infection by this polyoma virus (PV) to tumor development. All PV are potential transforming viruses, the large T-antigen of which interacts with tumor suppressor protein...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20429
更新日期:2006-03-01 00:00:00
abstract::Solitary fibrous tumor is a rare lesion of serosal membranes. In a pleural location, it may be confused clinically with a localized mesothelioma or a peripheral lung tumor. A transthoracic needle aspiration can be done to obtain material for a diagnosis in these easily accessible tumors. In the literature, there are o...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199802)18:2<118::aid-dc7>3
更新日期:1998-02-01 00:00:00
abstract::Seventy-four fine needle aspiration biopsies (FNAB) of breast were selected from the 133 cases with surgical biopsy follow up, from a total of 1,154 consecutive breast FNAB received in a 6-month-period. These 74 cases were reviewed and scored using all relevant cytological criteria for proliferative breast lesions use...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20653
更新日期:2007-08-01 00:00:00
abstract:BACKGROUND:The number of "renal incidentalomas" is on the rise due to increasing use of radiologic studies. Image-guided core needle biopsies (CNB) with touch preparations are performed to guide specimen collection and triage of sample for additional studies. Results allow the clinical team to make appropriate treatmen...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24253
更新日期:2019-10-01 00:00:00
abstract::Sinus histiocytosis with massive lymphadenopathy (SHML) is a rare entity characterized by the proliferation of cells belonging to the macrophage-histiocyte family, but whose exact origin is unknown. A case of SHML occurring in a 62-yr-old female is described in which the diagnosis is initially suggested by fine-needle...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840130413
更新日期:1995-11-01 00:00:00
abstract::Ultrasound examination (US) is an essential tool in the evaluation of thyroid nodules. The size determined by US is used to distinguish between clinical vs. nonclinical thyroid nodules i.e. greater than or equal to or less than 1 cm. In this study, we evaluated the concordance between the sizes of thyroid nodules meas...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20714
更新日期:2007-09-01 00:00:00
abstract::Involvement of the pancreas by metastatic sarcoma is rare, and can prove challenging to differentiate from sarcomatoid carcinomas which occur more commonly. The endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) technique has been successfully used for the diagnosis of pancreatic carcinomas whether primary ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21104
更新日期:2009-11-01 00:00:00
abstract::Extramedullary multiple myeloma (EMM) involving the liver as a focal space-occupying lesion is very rare, especially in the patients with cirrhosis. Here, we report a case of EMM in the liver and periportal lymph node, diagnosed by endoscopic ultrasound guided-fine-needle aspiration (EUS-FNA). A 57-year-old male patie...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24416
更新日期:2020-07-01 00:00:00
abstract::Cytologic preparations were made from 53 biopsies of osseous and related lesions submitted for intraoperative diagnosis. Smears made by scraping the surface of the lesion and spreading the material obtained on a glass slide were most commonly used. These preparations were found to be valuable adjuncts to frozen sectio...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840020103
更新日期:1986-01-01 00:00:00
abstract::Granulosa cell tumors (GCT) of the ovary are low grade tumor with a potential ability of late pelvic recurrences and distant metastases. However, there is sparse literature on the cytopathologic features of metastatic granulosa cell tumors (MGCT). Between 2000 and 2014, eight cases of MGCT were diagnosed by FNA. Clini...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23535
更新日期:2016-12-01 00:00:00
abstract::Mammary analogue secretory carcinoma (MASC) is a recently described salivary gland neoplasm that is defined by ETV6-NTRK3 gene fusion. There have been few case reports on the cytopathologic features of MASC to date. We examined the clinicopathological and cytological features of seven cases of MASC defined by RT-PCR a...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23139
更新日期:2014-10-01 00:00:00
abstract::Monoclonal antibodies were used for a preoperative analysis of estrogen receptors (ERs) and progesterone receptors (PgRs) in fine-needle aspirates from 44 primary human breast carcinomas. The semiquantitative receptor values obtained in cytologic specimens correlated well with those from enzyme immunoassay analysis on...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840060205
更新日期:1990-01-01 00:00:00
abstract::Over a 10-year period the Pap program at our community hospital laboratory progressed from the use of 100% conventional smears to predominantly ThinPrep (TP) and then to nearly 100% SurePath (SP). During this period the annual Pap volume grew from less than 20,000 to nearly 50,000. This retrospective review focuses on...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20607
更新日期:2007-03-01 00:00:00
abstract::We report a rare case of extranodal NK/T-cell lymphoma, nasal type of the uterine cervix that showed cytologic features mimicking cervical cancer. A 65-year-old woman presented with vaginal bleeding. Gynecological examination revealed a bulky tumor of the cervix. A conventional Papanicolaou-stained cervical smear show...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23439
更新日期:2016-05-01 00:00:00
abstract::Like a pulmonary counterpart, extrapulmonary small cell carcinoma (SCC) is an aggressive tumor with a high rate of metastasis. Forty-nine fine-needle aspiration biopsies (FNABs) (36 patients) of various primary sites other than the lung diagnosed as metastatic SCC (including Merkel cell carcinoma) were reviewed. FNABs...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199809)19:3<177::aid-dc4>3
更新日期:1998-09-01 00:00:00
abstract::Sequential tracheal aspirates from 39 neonates with hyaline membrane disease were examined to correlate cytological findings with the development of bronchopulmonary dysplasia (BPD). A total of 224 tracheal aspirates were examined from these infants, 15 of whom developed BPD as diagnosed by conventional clinical and r...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840020307
更新日期:1986-09-01 00:00:00
abstract::Differential leukocyte counts on noninflammatory synovial fluids (NISF) are not widely reported or used in research, apparently due to technical difficulties related to either high viscosity or low numbers of cells. We describe an evaluation of a technique using hyaluronidase and cytospin preparations to study NISF. T...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(200004)22:4<256::aid-dc13>
更新日期:2000-04-01 00:00:00
abstract::Pleomorphic xanthoastrocytoma (PXA) is a WHO grade II astrocytic tumor of children and young adults. It is characterized by pleomorphic, atypical astrocytes. Atypia is so remarkable, that PXA can be easily misdiagnosed as malignant glioma. If confused with a high-grade glioma the neurosurgeon may not proceed with a co...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23660
更新日期:2017-04-01 00:00:00
abstract::Myoepithelial cells play an important role in the interpretation of breast fine-needle aspiration biopsy, since these cells are believed to be a component of a benign process in breast lesions. Myoepithelial cells are usually easy to recognize, however, other cells can morphologically simulate myoepithelial cells and ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840130115
更新日期:1995-07-01 00:00:00
abstract::Ameloblastic fibroma of the jaw is a rare, benign mixed odontogenic tumor, having little tendency for local invasion and a low recurrence rate. Cytologic distinction from ameloblastoma, ameloblastic fibrosarcoma, and intraosseous adenoid cystic carcinoma is necessary, in view of the different biologic behavior. A pain...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.10310
更新日期:2003-08-01 00:00:00
abstract::Metastatic dissemination is the primary cause of death in ovarian cancer (OvCa) patients, and dissemination to pleural and peritoneal effusions is a common clinical event. Effusion samples were collected from 15 OvCa patients. Twenty-six samples were collected prospectively, two were archival, and eight were taken fro...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20279
更新日期:2005-11-01 00:00:00
abstract::Giant cell tumour (GCT) of bone is a distinctive neoplasm, which has only recently been included within the diagnostic purview of the cytopathologist. Four cases of GCT of bone diagnosed primarily and exclusively by fine needle aspiration cytology (FNAC), presenting at unusual sites, are presented with a view to highl...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.10188
更新日期:2002-12-01 00:00:00
abstract::Alzheimer's disease (AD) is the most common form of dementia. The cardinal histopathologic features include senile plaques (SPs) and neurofibrillary tangles (NFTs), and in addition, granulovacuolar degeneration (GVD) and Hirano bodies (HBs) are frequently observed in the hippocampus. We studied hippocampal cytological...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21053
更新日期:2009-09-01 00:00:00