The application of immunocytochemistry to direct smears in the diagnosis of effusions.

Abstract:

:Metastatic malignancy represents a common cause of effusions. Immunocytochemistry (ICC) is useful in confirming malignancy and gaining insight into the site of origin. Cell blocks are commonly utilized for this purpose; nonetheless, when the malignant cells are sparse, they may not be represented in cell blocks thereby precluding immunophenotypic characterization. Thus, we sought to investigate the utility of direct smear preparations as a platform for ICC in the diagnosis of effusions. Air-dried, unstained direct smears were prepared from 49 malignant effusions and 17 reactive effusions for comparison. ICC for EMA and MOC-31 highlighted the tumor cells in 91 and 98% of the malignant effusions tested, respectively. EMA immunoreactivity was focally observed within the calretinin-positive mesothelial cell population in 1 (6%) of the 17 reactive effusions. ICC for MOC-31 was negative in all reactive effusions. Site-specific immunomarkers were also evaluated. Immunoreactivity for Napsin-A and TTF-1 were observed in 78 and 67% of metastatic lung adenocarcinomas, respectively. ICC for PAX8 highlighted metastatic Müllerian and thyroid carcinomas in 100% of cases tested. CDX-2 immunoreactivity was observed in 25, 60, and 100% of metastatic gastric, pancreatic, and colorectal adenocarcinomas, respectively. Positivity for p63 was observed in 75% of metastatic urothelial cell carcinomas and the one case of pulmonary squamous cell carcinoma examined. Calretinin ICC highlighted the tumor cells in both malignant mesothelioma cases tested as well as the benign mesothelial cells in the reactive effusions. In conclusion, direct smears represent an effective platform for the performance of ICC in the diagnosis of malignant effusions.

journal_name

Diagn Cytopathol

journal_title

Diagnostic cytopathology

authors

Knoepp SM,Placido J,Fields KL,Thomas D,Roh MH

doi

10.1002/dc.22852

subject

Has Abstract

pub_date

2013-05-01 00:00:00

pages

425-30

issue

5

eissn

8755-1039

issn

1097-0339

journal_volume

41

pub_type

杂志文章
  • Routine Pap smears for the diagnosis of bacterial vaginosis.

    abstract::This study investigated the role of Papanicolaou (Pap) smears in determining the presence of bacterial vaginosis (BV). Pap results, vaginal Gram stains, signs, symptoms, and wet mounts were evaluated. Vaginal smears for Gram stain and routine Pap smears were collected from 420 consecutive patients. Ninety-three percen...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199907)21:1<10::aid-dc4>3.

    authors: Prey M

    更新日期:1999-07-01 00:00:00

  • The utility of ancillary studies in pediatric FNA cytology.

    abstract::We evaluated the diagnostic contribution of adjunct studies performed on aspirated material in the work-up of pediatric fine-needle aspiration (FNA) biopsies. Ancillary studies were performed on 54 of 136 (39.7%) pediatric FNA biopsies during a 5-year period. In 23 (16.9%) cases, immunocytochemical (ICC) studies, cons...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840080210

    authors: Gurley AM,Silverman JF,Lassaletta MM,Wiley JE,Holbrook CT,Joshi VV

    更新日期:1992-01-01 00:00:00

  • Cytopathology of uncommon malignant renal neoplasms in the pediatric age group.

    abstract::Malignant renal neoplasms are common solid tumors in pediatric oncology practice. These include the common Wilms' tumor/nephroblastoma and the uncommon neoplasms such as clear-cell sarcoma of the kidney (CCSK), rhabdoid tumor, renal-cell carcinoma, and others. The aim of this study was to describe in detail the cytopa...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20242

    authors: Radhika S,Bakshi A,Rajwanshi A,Nijhawan R,Das A,Kakkar N,Joshi K,Marwaha RK,Rao KL

    更新日期:2005-05-01 00:00:00

  • How "suspicious" is that nodule? Review of "suspicious" Afirma gene expression classifier in high risk thyroid nodules.

    abstract:BACKGROUND:The Afirma gene expression classifier (GEC) is being increasingly utilized to confirm the benign nature of indeterminate FNA cytology results thus avoiding unnecessary surgical procedures. However the "suspicious" result of the Afirma GEC does not classify these indeterminate nodules further in determining a...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23680

    authors: Roychoudhury S,Klein M,Souza F,Gimenez C,Laser A,Shaheen Cocker R,Chau K,Das K

    更新日期:2017-04-01 00:00:00

  • Cytologic characteristics of clear-cell sarcoma of the kidney (CCSK) in fine-needle aspiration biopsy (FNAB): a report of 4 cases.

    abstract::The cytologic findings observed in fine-needle aspiration biopsy (FNAB) of four cases of clear-cell sarcoma of the kidney (CCSK) are reported. Smears contained a monomorphic population of cells exhibiting non-descriptive cytoplasm and almost nude round to oval nuclei with smooth contour, evenly dispersed fine chromati...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840070612

    authors: Drut R,Pomar M

    更新日期:1991-01-01 00:00:00

  • Applications of flow cytometric DNA analysis to diagnostic cytology.

    abstract::Flow cytometry is a rapid method for measuring DNA content. Its high sensitivity and specificity in detecting transitional cell carcinomas in bladder washings should make it a routinely applied adjunct to cytology once universal laboratory standards are firmly established. Diagnostic and prognostic information on soli...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.2840130219

    authors: Cibas ES

    更新日期:1995-08-01 00:00:00

  • Hürthle cell metaplasia in chronic lymphocytic thyroiditis: Role of age factor and review of literature on its molecular pathogenesis.

    abstract:OBJECTIVES:Hürthle cell (HC) metaplasia is an important feature of chronic lymphocytic (Hashimoto) thyroiditis (HT). However, it is rarely observed in juvenile form HT. In a study based on fine needle aspiration cytology, the cytomorphological difference between pediatric HT and its adult form was studied, with special...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.24140

    authors: Das DK

    更新日期:2019-05-01 00:00:00

  • Intraabdominal desmoplastic small round cell tumor: cytopathologic findings in two cases.

    abstract::Intraabdominal desmoplastic small round cell tumor (DSRCT) is an extremely rare entity. This study describes fine-needle aspiration, ascitic fluid, and touch imprint cytomorphology of DSRCT in 2 patients with extensive abdomino-pelvic disease. Cytopathologic features were unique and showed good morphologic correlation...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199806)18:6<449::aid-dc13>

    authors: Ali SZ,Nicol TL,Port J,Ford G

    更新日期:1998-06-01 00:00:00

  • American Society of Cytopathology workload recommendations for automated Pap test screening: developed by the productivity and quality assurance in the era of automated screening task force.

    abstract::Based on current literature and the best available research to date, the current FDA workload limits for automated image-assisted screening, including the ThinPrep Imaging System and the FocalPoint GS, of 100 slides/day (imaged only slides counted as 0.5) are extremely high and may be associated with significant reduc...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,实务指引

    doi:10.1002/dc.22817

    authors: Elsheikh TM,Austin RM,Chhieng DF,Miller FS,Moriarty AT,Renshaw AA,American Society of Cytopathology.

    更新日期:2013-02-01 00:00:00

  • Chordoma: cytologic and immunocytochemical study of four cases.

    abstract::We present the cytologic features and the immunocytochemical profile of four cases of chordoma on fine-needle aspiration biopsies. The physaliferous cells in signet-ring, pearl-like formations and the trabecular arrangement with rounded contours are distinctive. Other cell types and cellular arrangements are also desc...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840020112

    authors: Kontozoglou T,Qizilbash AH,Sianos J,Stead R

    更新日期:1986-01-01 00:00:00

  • Pancreatic lymphoma: A cytologic diagnosis challenge.

    abstract::Very rarely lymphoma primarily or secondarily involves the pancreas. Involvement of the pancreatic parenchyma with lymphoma clinically may mimic pancreatic ductal adenocarcinoma (PDA) and other mass-forming pancreatic lesions. Endoscopic ultrasound fine needle aspiration (EUS-FNA) is the first step in the diagnostic p...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24349

    authors: Savari O,Al-Duwal Z,Wang Z,Ganesan S,Danan-Rayes R,Ayub S

    更新日期:2020-04-01 00:00:00

  • Effectiveness of fine-needle aspiration cytology of breast: analysis of 2,375 cases from northern Thailand.

    abstract::At the Maharaj Nakorn Chiang Mai Hospital, Chiang Mai, Thailand, 2,375 cases of breast lesions were sampled by fine-needle aspiration (FNA) from 1994-1999. Cytologic diagnoses were: benign (48%), suspicious for malignancy (5%), malignant (15%), and unsatisfactory (32%). Comparison with histology was possible in 721 ca...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10067

    authors: Chaiwun B,Settakorn J,Ya-In C,Wisedmongkol W,Rangdaeng S,Thorner P

    更新日期:2002-03-01 00:00:00

  • Parathyroid fine-needle aspiration cytology in the evaluation of parathyroid adenoma: cytologic findings from 53 patients.

    abstract::This study was designed to assess the utility of fine-needle aspiration cytology (FNAC) in the preoperative localization of parathyroid adenoma (PA). Fifty-seven samples from fifty-three cases of PA (four patients had bilateral disease) were obtained by ultrasound (US)-guided fine-needle aspiration. Parathyroid hormon...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21020

    authors: Agarwal AM,Bentz JS,Hungerford R,Abraham D

    更新日期:2009-06-01 00:00:00

  • Intranuclear peudoinclusions: morphology, pathogenesis, and significance.

    abstract::Intranuclear pseudoinclusions represent invaginations of the cytoplasm into the nucleus. The nuclear pseudoinclusions are generally easier to appreciate in cytological preparations than frozen sections or histopathology thus forming an important cytological feature. In the present article we have reviewed the morpholo...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.21714

    authors: Arora SK,Dey P

    更新日期:2012-08-01 00:00:00

  • Concurrent pleural infiltration by chronic lymphocytic leukemia and adenocarcinoma of unknown primary site diagnosed by effusion cytology.

    abstract::Synchronous malignancies in a pleural effusion are rare. A case of concurrent pleural infiltration by adenocarcinoma of unknown primary site and chronic lymphocytic leukemia (CLL) is presented in this case study, which was diagnosed by effusion cytology. Pleural effusion is not an uncommon complication in patients wit...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.22889

    authors: Vrettos I,Kamposioras K,Peridis S,Aninos D,Kazika S,Spathis A,Karakitsos P,Papadopoulos A

    更新日期:2014-02-01 00:00:00

  • Malignant perivascular epithelioid tumor of the vagina: Report of a rare case with brief review of literature.

    abstract::Perivascular epithelioid cell tumors (PEComas) are rare mesenchymal tumors with immunohistochemical co-expression of melanocytic and myoid markers. Vaginal PEComas have been described in only nine cases so far. We describe the case of a 65-year-old female with a large growth in the left lateral vaginal wall. Biopsy im...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.24402

    authors: Gupta R,Hariprasad R,Dhanasekaran K,Gupta S

    更新日期:2020-05-01 00:00:00

  • Combining fine needle aspiration with brushing cytology has improved yields in diagnosing pancreatic ductal adenocarcinoma.

    abstract::The aim of this retrospective study is to evaluate the diagnostic yields of combining fine needle aspiration (FNA) with brushing cytology (BC) in clinical work-up of pancreatic ductal adenocarcinoma. The study included a total of 97 patients who underwent both FNA and BC along with histologic/clinical follow-up (F/U)....

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21062

    authors: Jing X,Wamsteker EJ,Li H,Pu RT

    更新日期:2009-08-01 00:00:00

  • Urothelial toxicity following conditioning therapy in bone marrow transplantation and bladder cancer: morphologic and morphometric comparison by exfoliative urinary cytology.

    abstract::Since cyclophosphamide and busulphan used for therapy of bone marrow transplantation (BMT) can cause urothelial cell changes similar to those found in bladder cancer, comparative morphologic and morphometric urinary cytologic research was carried out, examining 812 urine samples taken from 121 patients undergoing BMT ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840080306

    authors: Stella F,Battistelli S,Marcheggiani F,De Santis M,Donnorso RP,Baronciani D,Durazzi SM,Troccoli R,Lucarelli G

    更新日期:1992-01-01 00:00:00

  • Malignant phyllodes tumor with chondrosarcomatous differentiation: report of a case with cytological presentation.

    abstract::Malignant phyllodes tumor is a rare breast tumor with neoplastic epithelial and stromal components. The stromal component may show homologous and heterologous sarcomatous elements, including chondrosarcomatous and osteosarcomatous differentiation. Because these tumors may present with an almost exclusively sarcomatous...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199904)20:4<241::aid-dc12>

    authors: Garcia FU,Soans S,Galindo LM

    更新日期:1999-04-01 00:00:00

  • Cytodiagnostic patterns of metaplastic breast carcinoma in aspiration samples: a study of 14 cases.

    abstract::Metaplastic breast carcinomas (MBCs) are not only uncommon neoplasms but also show a myriad of cytomorphologic patterns in aspiration samples. These tumors comprise less than 5% of breast malignancies and have an uncertain prognosis. In this study, the fine-needle aspiration cytologic features of 14 MBCs with the iden...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199901)20:1<10::aid-dc3>3.

    authors: Gupta RK

    更新日期:1999-01-01 00:00:00

  • Cervical tuberculosis detection in Papanicolaou-stained smear: case report with review of literature.

    abstract::Genital tract tuberculosis is usually secondary to an extragenital tubercular focus. Cervical tuberculosis, however, is rare accounting for up to 3% of all genital tract diseases. The diagnosis of tuberculosis is usually established on multiple cervical biopsies followed by bacteriological confirmation. There are only...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.21082

    authors: Gupta R,Dey P,Jain V,Gupta N

    更新日期:2009-08-01 00:00:00

  • Fine-needle aspiration biopsy findings in sclerosing polycystic adenosis of the parotid gland.

    abstract::Sclerosing polycystic adenosis (SPA) is a recently described, rare lesion of the salivary gland analogous to fibrocystic disease of the breast. Recognition of this benign entity is important since the differential diagnosis includes other more common benign and malignant salivary gland neoplasms, particularly mucoepid...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20671

    authors: Etit D,Pilch BZ,Osgood R,Faquin WC

    更新日期:2007-07-01 00:00:00

  • Progression of cervical lesions in HIV-seropositive women: a cytological study.

    abstract::It has been reported that cervical intraepithelial lesions have a more aggressive course in HIV-seropositive than in HIV-seronegative women. In the present investigation, the progression of these cervical lesions was studied in a group of HIV-seropositive women. Of 1,587 patients, 200 (12.6%) had a cytological diagnos...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/1097-0339(200102)24:2<117::aid-dc1021>3.0.

    authors: Calore EE,Pereira SM,Cavaliere MJ

    更新日期:2001-02-01 00:00:00

  • Primary salivary gland-type tumors of the tracheobronchial tree diagnosed by transbronchial fine needle aspiration: Clinical and cytomorphologic features with histopathologic correlation.

    abstract:BACKGROUND:Primary salivary gland-type tumors of the tracheobronchial tree are rare; their cytologic features have been seldom reported. We aim to describe the clinical and cytomorphologic features of tracheobronchial salivary gland-type tumors diagnosed by transbronchial fine needle aspiration (TBNA) at our institutio...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24285

    authors: Doxtader EE,Shah AA,Zhang Y,Wang H,Dyhdalo KS,Farver C

    更新日期:2019-11-01 00:00:00

  • Fine-needle aspiration cytology of hepatic leiomyosarcoma.

    abstract::Hepatic leiomyosarcoma is a rare malignancy to involve the liver, occurring as a primary liver sarcoma in patients without an underlying medical disorder, as a metastatic malignancy, and with increasing incidence, as a primary tumor in AIDS patients. A series of hepatic leiomyosarcomas diagnosed by FNA biopsy, includi...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840110403

    authors: Smith MB,Silverman JF,Raab SS,Towell BD,Geisinger KR

    更新日期:1994-12-01 00:00:00

  • Diffuse large B-cell lymphoma with fibrillary matrix.

    abstract::Diffuse large B-cell lymphoma (DLBCL) with fibrillary matrix is an extremely rare variant of non-Hodgkin's lymphoma (NHL) whose pathological features are poorly known. Here, we report on our experience with such a condition arising in a 71-yr-old woman. Our findings not only demonstrate that DLBCL with fibrillary matr...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10172

    authors: Dargent JL,Meiers I,Lespagnard L,Ma Y,Dehou MF,Verhest A

    更新日期:2002-10-01 00:00:00

  • Fine-needle aspiration of hepatocellular carcinoma: False-negative result due to epithelioid cells.

    abstract::The presence of epithelioid cells in fine-needle aspirations of a liver nodule is rare, but may complicate the diagnosis of the nodule. We report on a case of a liver nodule in hepatitis C cirrhosis. Results of fine-needle aspiration mainly revealed the presence of epithelioid cells, without any recognizable tumor cel...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.1087

    authors: Mourra N,Flejou JF

    更新日期:2001-06-01 00:00:00

  • Chlamydia trachomatis in cervical uterine irradiated cancer patients.

    abstract::Twenty-two women with uterine carcinoma with morphological alterations postradiation treatment suggestive of Chlamydia trachomatis infection were analyzed by direct immunofluorescence. The diagnosis was confirmed in 12 (54.4%) cases. Discussions of the presence of Chlamydia in irradiated cells were not found in the li...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840060203

    authors: Maeda MY,Longatto Filho A,Shih LW,Cavaliere MJ,Oyafuso MS,Marziona F,Santos DR,Carvalho MI

    更新日期:1990-01-01 00:00:00

  • Clinical significance of cultures collected from fine-needle aspiration biopsy.

    abstract::The rate of positive cultures in fine-needle aspiration biopsy (FNAB) specimens is evaluated, and the value of submitting FNAB culture is assessed. Review of 3,300 FNAB specimens from 2,416 patients were tabulated for culture results, when obtained from the FNAB material. For positive culture results, clinical impact ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20648

    authors: Granville LA,Laucirica R,Verstovsek G

    更新日期:2008-02-01 00:00:00

  • Bladder-washing cytology of metastatic ovarian granulosa cell tumor.

    abstract::An 86-year-old Caucasian female presented with two weeks history of discomfort discharging urine, occasional hematuria, and suprapubic pain. The patient had a history of left salpingo-oophorectomy for an ovarian tumor, performed four years earlier. Ultrasound showed a solid mass surrounding the orifice of the left ure...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10095

    authors: Vodovnik A

    更新日期:2002-06-01 00:00:00