Abstract:
:Metastatic malignancy represents a common cause of effusions. Immunocytochemistry (ICC) is useful in confirming malignancy and gaining insight into the site of origin. Cell blocks are commonly utilized for this purpose; nonetheless, when the malignant cells are sparse, they may not be represented in cell blocks thereby precluding immunophenotypic characterization. Thus, we sought to investigate the utility of direct smear preparations as a platform for ICC in the diagnosis of effusions. Air-dried, unstained direct smears were prepared from 49 malignant effusions and 17 reactive effusions for comparison. ICC for EMA and MOC-31 highlighted the tumor cells in 91 and 98% of the malignant effusions tested, respectively. EMA immunoreactivity was focally observed within the calretinin-positive mesothelial cell population in 1 (6%) of the 17 reactive effusions. ICC for MOC-31 was negative in all reactive effusions. Site-specific immunomarkers were also evaluated. Immunoreactivity for Napsin-A and TTF-1 were observed in 78 and 67% of metastatic lung adenocarcinomas, respectively. ICC for PAX8 highlighted metastatic Müllerian and thyroid carcinomas in 100% of cases tested. CDX-2 immunoreactivity was observed in 25, 60, and 100% of metastatic gastric, pancreatic, and colorectal adenocarcinomas, respectively. Positivity for p63 was observed in 75% of metastatic urothelial cell carcinomas and the one case of pulmonary squamous cell carcinoma examined. Calretinin ICC highlighted the tumor cells in both malignant mesothelioma cases tested as well as the benign mesothelial cells in the reactive effusions. In conclusion, direct smears represent an effective platform for the performance of ICC in the diagnosis of malignant effusions.
journal_name
Diagn Cytopatholjournal_title
Diagnostic cytopathologyauthors
Knoepp SM,Placido J,Fields KL,Thomas D,Roh MHdoi
10.1002/dc.22852subject
Has Abstractpub_date
2013-05-01 00:00:00pages
425-30issue
5eissn
8755-1039issn
1097-0339journal_volume
41pub_type
杂志文章abstract::This study investigated the role of Papanicolaou (Pap) smears in determining the presence of bacterial vaginosis (BV). Pap results, vaginal Gram stains, signs, symptoms, and wet mounts were evaluated. Vaginal smears for Gram stain and routine Pap smears were collected from 420 consecutive patients. Ninety-three percen...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199907)21:1<10::aid-dc4>3.
更新日期:1999-07-01 00:00:00
abstract::We evaluated the diagnostic contribution of adjunct studies performed on aspirated material in the work-up of pediatric fine-needle aspiration (FNA) biopsies. Ancillary studies were performed on 54 of 136 (39.7%) pediatric FNA biopsies during a 5-year period. In 23 (16.9%) cases, immunocytochemical (ICC) studies, cons...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840080210
更新日期:1992-01-01 00:00:00
abstract::Malignant renal neoplasms are common solid tumors in pediatric oncology practice. These include the common Wilms' tumor/nephroblastoma and the uncommon neoplasms such as clear-cell sarcoma of the kidney (CCSK), rhabdoid tumor, renal-cell carcinoma, and others. The aim of this study was to describe in detail the cytopa...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20242
更新日期:2005-05-01 00:00:00
abstract:BACKGROUND:The Afirma gene expression classifier (GEC) is being increasingly utilized to confirm the benign nature of indeterminate FNA cytology results thus avoiding unnecessary surgical procedures. However the "suspicious" result of the Afirma GEC does not classify these indeterminate nodules further in determining a...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23680
更新日期:2017-04-01 00:00:00
abstract::The cytologic findings observed in fine-needle aspiration biopsy (FNAB) of four cases of clear-cell sarcoma of the kidney (CCSK) are reported. Smears contained a monomorphic population of cells exhibiting non-descriptive cytoplasm and almost nude round to oval nuclei with smooth contour, evenly dispersed fine chromati...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840070612
更新日期:1991-01-01 00:00:00
abstract::Flow cytometry is a rapid method for measuring DNA content. Its high sensitivity and specificity in detecting transitional cell carcinomas in bladder washings should make it a routinely applied adjunct to cytology once universal laboratory standards are firmly established. Diagnostic and prognostic information on soli...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.2840130219
更新日期:1995-08-01 00:00:00
abstract:OBJECTIVES:Hürthle cell (HC) metaplasia is an important feature of chronic lymphocytic (Hashimoto) thyroiditis (HT). However, it is rarely observed in juvenile form HT. In a study based on fine needle aspiration cytology, the cytomorphological difference between pediatric HT and its adult form was studied, with special...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.24140
更新日期:2019-05-01 00:00:00
abstract::Intraabdominal desmoplastic small round cell tumor (DSRCT) is an extremely rare entity. This study describes fine-needle aspiration, ascitic fluid, and touch imprint cytomorphology of DSRCT in 2 patients with extensive abdomino-pelvic disease. Cytopathologic features were unique and showed good morphologic correlation...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199806)18:6<449::aid-dc13>
更新日期:1998-06-01 00:00:00
abstract::Based on current literature and the best available research to date, the current FDA workload limits for automated image-assisted screening, including the ThinPrep Imaging System and the FocalPoint GS, of 100 slides/day (imaged only slides counted as 0.5) are extremely high and may be associated with significant reduc...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,实务指引
doi:10.1002/dc.22817
更新日期:2013-02-01 00:00:00
abstract::We present the cytologic features and the immunocytochemical profile of four cases of chordoma on fine-needle aspiration biopsies. The physaliferous cells in signet-ring, pearl-like formations and the trabecular arrangement with rounded contours are distinctive. Other cell types and cellular arrangements are also desc...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840020112
更新日期:1986-01-01 00:00:00
abstract::Very rarely lymphoma primarily or secondarily involves the pancreas. Involvement of the pancreatic parenchyma with lymphoma clinically may mimic pancreatic ductal adenocarcinoma (PDA) and other mass-forming pancreatic lesions. Endoscopic ultrasound fine needle aspiration (EUS-FNA) is the first step in the diagnostic p...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24349
更新日期:2020-04-01 00:00:00
abstract::At the Maharaj Nakorn Chiang Mai Hospital, Chiang Mai, Thailand, 2,375 cases of breast lesions were sampled by fine-needle aspiration (FNA) from 1994-1999. Cytologic diagnoses were: benign (48%), suspicious for malignancy (5%), malignant (15%), and unsatisfactory (32%). Comparison with histology was possible in 721 ca...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.10067
更新日期:2002-03-01 00:00:00
abstract::This study was designed to assess the utility of fine-needle aspiration cytology (FNAC) in the preoperative localization of parathyroid adenoma (PA). Fifty-seven samples from fifty-three cases of PA (four patients had bilateral disease) were obtained by ultrasound (US)-guided fine-needle aspiration. Parathyroid hormon...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21020
更新日期:2009-06-01 00:00:00
abstract::Intranuclear pseudoinclusions represent invaginations of the cytoplasm into the nucleus. The nuclear pseudoinclusions are generally easier to appreciate in cytological preparations than frozen sections or histopathology thus forming an important cytological feature. In the present article we have reviewed the morpholo...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.21714
更新日期:2012-08-01 00:00:00
abstract::Synchronous malignancies in a pleural effusion are rare. A case of concurrent pleural infiltration by adenocarcinoma of unknown primary site and chronic lymphocytic leukemia (CLL) is presented in this case study, which was diagnosed by effusion cytology. Pleural effusion is not an uncommon complication in patients wit...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.22889
更新日期:2014-02-01 00:00:00
abstract::Perivascular epithelioid cell tumors (PEComas) are rare mesenchymal tumors with immunohistochemical co-expression of melanocytic and myoid markers. Vaginal PEComas have been described in only nine cases so far. We describe the case of a 65-year-old female with a large growth in the left lateral vaginal wall. Biopsy im...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.24402
更新日期:2020-05-01 00:00:00
abstract::The aim of this retrospective study is to evaluate the diagnostic yields of combining fine needle aspiration (FNA) with brushing cytology (BC) in clinical work-up of pancreatic ductal adenocarcinoma. The study included a total of 97 patients who underwent both FNA and BC along with histologic/clinical follow-up (F/U)....
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21062
更新日期:2009-08-01 00:00:00
abstract::Since cyclophosphamide and busulphan used for therapy of bone marrow transplantation (BMT) can cause urothelial cell changes similar to those found in bladder cancer, comparative morphologic and morphometric urinary cytologic research was carried out, examining 812 urine samples taken from 121 patients undergoing BMT ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840080306
更新日期:1992-01-01 00:00:00
abstract::Malignant phyllodes tumor is a rare breast tumor with neoplastic epithelial and stromal components. The stromal component may show homologous and heterologous sarcomatous elements, including chondrosarcomatous and osteosarcomatous differentiation. Because these tumors may present with an almost exclusively sarcomatous...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199904)20:4<241::aid-dc12>
更新日期:1999-04-01 00:00:00
abstract::Metaplastic breast carcinomas (MBCs) are not only uncommon neoplasms but also show a myriad of cytomorphologic patterns in aspiration samples. These tumors comprise less than 5% of breast malignancies and have an uncertain prognosis. In this study, the fine-needle aspiration cytologic features of 14 MBCs with the iden...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199901)20:1<10::aid-dc3>3.
更新日期:1999-01-01 00:00:00
abstract::Genital tract tuberculosis is usually secondary to an extragenital tubercular focus. Cervical tuberculosis, however, is rare accounting for up to 3% of all genital tract diseases. The diagnosis of tuberculosis is usually established on multiple cervical biopsies followed by bacteriological confirmation. There are only...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.21082
更新日期:2009-08-01 00:00:00
abstract::Sclerosing polycystic adenosis (SPA) is a recently described, rare lesion of the salivary gland analogous to fibrocystic disease of the breast. Recognition of this benign entity is important since the differential diagnosis includes other more common benign and malignant salivary gland neoplasms, particularly mucoepid...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20671
更新日期:2007-07-01 00:00:00
abstract::It has been reported that cervical intraepithelial lesions have a more aggressive course in HIV-seropositive than in HIV-seronegative women. In the present investigation, the progression of these cervical lesions was studied in a group of HIV-seropositive women. Of 1,587 patients, 200 (12.6%) had a cytological diagnos...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/1097-0339(200102)24:2<117::aid-dc1021>3.0.
更新日期:2001-02-01 00:00:00
abstract:BACKGROUND:Primary salivary gland-type tumors of the tracheobronchial tree are rare; their cytologic features have been seldom reported. We aim to describe the clinical and cytomorphologic features of tracheobronchial salivary gland-type tumors diagnosed by transbronchial fine needle aspiration (TBNA) at our institutio...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24285
更新日期:2019-11-01 00:00:00
abstract::Hepatic leiomyosarcoma is a rare malignancy to involve the liver, occurring as a primary liver sarcoma in patients without an underlying medical disorder, as a metastatic malignancy, and with increasing incidence, as a primary tumor in AIDS patients. A series of hepatic leiomyosarcomas diagnosed by FNA biopsy, includi...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840110403
更新日期:1994-12-01 00:00:00
abstract::Diffuse large B-cell lymphoma (DLBCL) with fibrillary matrix is an extremely rare variant of non-Hodgkin's lymphoma (NHL) whose pathological features are poorly known. Here, we report on our experience with such a condition arising in a 71-yr-old woman. Our findings not only demonstrate that DLBCL with fibrillary matr...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.10172
更新日期:2002-10-01 00:00:00
abstract::The presence of epithelioid cells in fine-needle aspirations of a liver nodule is rare, but may complicate the diagnosis of the nodule. We report on a case of a liver nodule in hepatitis C cirrhosis. Results of fine-needle aspiration mainly revealed the presence of epithelioid cells, without any recognizable tumor cel...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.1087
更新日期:2001-06-01 00:00:00
abstract::Twenty-two women with uterine carcinoma with morphological alterations postradiation treatment suggestive of Chlamydia trachomatis infection were analyzed by direct immunofluorescence. The diagnosis was confirmed in 12 (54.4%) cases. Discussions of the presence of Chlamydia in irradiated cells were not found in the li...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840060203
更新日期:1990-01-01 00:00:00
abstract::The rate of positive cultures in fine-needle aspiration biopsy (FNAB) specimens is evaluated, and the value of submitting FNAB culture is assessed. Review of 3,300 FNAB specimens from 2,416 patients were tabulated for culture results, when obtained from the FNAB material. For positive culture results, clinical impact ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20648
更新日期:2008-02-01 00:00:00
abstract::An 86-year-old Caucasian female presented with two weeks history of discomfort discharging urine, occasional hematuria, and suprapubic pain. The patient had a history of left salpingo-oophorectomy for an ovarian tumor, performed four years earlier. Ultrasound showed a solid mass surrounding the orifice of the left ure...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.10095
更新日期:2002-06-01 00:00:00