Abstract:
:The aim of our study was to evaluate the levels of fibronectin (FN) and its classic receptor (FNR) in various transformed cells lines, especially of leukemic origin, and also the influence of HIV-1 replication on the expression of these proteins (in particular on H9-V cells, chronically infected with HIV-1, and acutely infected MT-4 cells). Monoclonal antibodies were used for indirect immunofluorescence tests; the fluorescein-conjugated recombinant p14, the product of the HIV gene tat, was used as a molecular probe. The results demonstrated a high variability of FN and FNR expression among the various cellular lines studied. Moreover, deficits of such adhesive proteins did not necessarily correlate with a severe reduction of the corresponding receptor. HIV-1 replication in MT-4 and H9-V cells increased the expression of FNR. This seems to correlate with p14-induced phenomena because pretreatment of H9-V cells with recombinant p14 showed an enhancing effect on the expression of this receptor.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Pugliese A,Comito G,Savarino A,Cantamessa C,Torre Ddoi
10.1002/cbf.665subject
Has Abstractpub_date
1996-06-01 00:00:00pages
105-10issue
2eissn
0263-6484issn
1099-0844pii
10.1002/(SICI)1099-0844(199606)14:2<105::AID-CBF66journal_volume
14pub_type
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