Abstract:
:The objectives of study were (a) to determine alteration of feeding, glucose level and oxidative stress and (b) to investigate expression and localization of cannabinoid receptors in type-2 diabetic rat pancreas treated with Δ(9)-tetrahydrocannabinol (Δ(9)-THC). Rats were randomly divided into four groups: control, Δ(9)-THC, diabetes and diabetes + Δ(9)-THC groups. Diabetic rats were treated with a single dose of nicotinamide (85 mg/kg) 15 min before injection of streptozotocin (65 mg/kg). Δ(9)-THC was administered intraperitoneally at 3 mg/kg/day for 7 days. Body weights and blood glucose level of rats in all groups were measured on days 0, 7, 14 and 21. On day 15 after the Δ(9)-THC injections, pancreatic tissues were removed. Blood glucose levels and body weights of diabetic rats treated with Δ(9)-THC did not show statistically significant changes when compared with the diabetic animals on days 7, 14 and 21. Treatment with Δ(9)-THC significantly increased pancreas glutathione levels, enzyme activities of superoxide dismutase and catalase in diabetes compared with non-treatment diabetes group. The cannabinoid 1 receptor was found in islets, whereas the cannabinoid 2 receptor was found in pancreatic ducts. Their localization in cells was both nuclear and cytoplasmic. We can suggest that Δ(9) -THC may be an important agent for the treatment of oxidative damages induced by diabetes. However, it must be supported with anti-hyperglycaemic agents. Furthermore, the present study for the first time emphasizes that Δ(9)-THC may improve pancreatic cells via cannabinoid receptors in diabetes. The aim of present study was to elucidate the effects of Δ(9)-THC, a natural cannabinoid receptor agonist, on the expression and localization of cannabinoid receptors, and oxidative stress statue in type-2 diabetic rat pancreas. Results demonstrate that the cannabinoid receptors are presented in both Langerhans islets and duct regions. The curative effects of Δ(9)-THC can be occurred via activation of cannabinoid receptors in diabetic rat pancreas. Moreover, it may provide a protective effect against oxidative damage induced by diabetes. Thus, it is suggested that Δ(9)-THC can be a candidate for therapeutic alternatives of diabetes symptoms.
journal_name
Cell Biochem Functjournal_title
Cell biochemistry and functionauthors
Coskun ZM,Bolkent Sdoi
10.1002/cbf.3058subject
Has Abstractpub_date
2014-10-01 00:00:00pages
612-9issue
7eissn
0263-6484issn
1099-0844journal_volume
32pub_type
杂志文章abstract::The aim of the present studies was to determine whether the mechanism of biological action of garlic-derived sulfur compounds in human hepatoma (HepG2) cells can be dependent on the presence of labile sulfane sulfur in their molecules. We investigated the effect of allyl sulfides from garlic: monosulfide, disulfide an...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1835
更新日期:2012-04-01 00:00:00
abstract::Rat liver mitochondria were stored at 0-4 degrees C for several days using an appropriate medium and energy source. The elimination of the majority of microsomes and lysosomes, that normally contaminate isolated mitochondria, had a positive effect in preservation of respiratory control, P:O ratio, and monoamine oxidas...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290030407
更新日期:1985-10-01 00:00:00
abstract::UFD1L is the human homologue of the yeast ubiquitin fusion degradation 1 (Ufd1) gene and maps on chromosome 22q11.2 in the typically deleted region (TDR) for DiGeorge/velocardiofacial syndromes (DGS/VCFS). In yeast, Ufd1 protein is involved in a degradation pathway for ubiquitin fused products (UFD pathway). Several s...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1021
更新日期:2003-09-01 00:00:00
abstract::The protective effects of betaine in ethanol hepatotoxicity were investigated in 24 female wistar albino rats. Animals were divided into three groups: control, ethanol and ethanol + betaine group. Animals were fed liquid diets and consumed approximately 60 diet per day. Rats were fed ethanol 8 kg(- 1) day(- 1). The et...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.926
更新日期:2001-12-01 00:00:00
abstract::We examined the influence of chronic treatment with KN-93 (an inhibitor of Ca(2+)/calmodulin-dependent protein kinase II (CaMKII), 5 mg/kg given every other day for 4 weeks) on mean arterial pressure (MAP), urine protein and vascular reactivity in streptozotocin (STZ)-induced diabetes in Wistar Kyoto (WKY) and spontan...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1446
更新日期:2008-03-01 00:00:00
abstract::Betulin is a principal component of birch bark and is known to possess a broad range of biological activities, including antiinflammatory, antiviral and anticancer actions. The present study was carried out in vitro to clarify the influence of betulin on melanocortin (MC) receptor-ergic signalling by using COS-7 cells...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1427
更新日期:2007-09-01 00:00:00
abstract::The ataxia-telangiectasia mutated (ATM) protein kinase is best known for its critical nuclear roles in the DNA damage response (DDR), cell cycle checkpoints, and the maintenance of gene stability. In this review, we highlight the multifaceted cytoplasmic functions of ATM in autophagy. We focused on the functions of AT...
journal_title:Cell biochemistry and function
pub_type: 杂志文章,评审
doi:10.1002/cbf.3385
更新日期:2019-04-01 00:00:00
abstract::The effects of either radiation or hyperthermia on the differentiation potential of NG108-15, a neuroblastoma-glioma hybrid cell line, were studied. After radiation and hyperthermia, the outgrowth of neurites from NG108-15 cells was potentiated, and polarizing current and voltage pulses induced a distinct action poten...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290120209
更新日期:1994-06-01 00:00:00
abstract::The aim of the present study was to measure plasma uric acid (UA) levels and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and xanthine oxidase (XO) activities and to evaluate the relationship between these parameters and the severity of pre-eclampsia. Twenty-five pre-eclamptic, 15 healthy pregnant and 1...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1090
更新日期:2004-07-01 00:00:00
abstract::Because elevated oxidative stress may exacerbate cardiovascular complications of diabetes mellitus, the current study aimed to investigate the effects of treatment with either vitamin A, an antioxidant, or with insulin on lipid peroxidation products and antioxidant enzyme activities of diabetic rat heart. Also to eval...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.957
更新日期:2002-06-01 00:00:00
abstract::The molecular mechanisms involved in GPCR-initiated signaling cascades where the two receptors share the same signaling cascade, such as thyrotropin-releasing hormone (TRH) and angiotensin II (ANG II), are still far from being understood. Here, we analyzed hormone-induced Ca(2+) responses and the process of desensitiz...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1453
更新日期:2008-03-01 00:00:00
abstract::This study was designed to investigate the effect of L-carnitine in ischaemia and reperfusion of the rat kidney. Rats were randomly allocated into three groups. Group I (control group; n = 6) received no treatment. Group II (isotonic saline group; n = 6), received 2 ml of isotonic saline 15 min before the renal ischae...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1159
更新日期:2005-05-01 00:00:00
abstract::Reduced and oxidized glutathione (GSH and GSSG), protein-bound glutathione, lipid peroxidation and antioxidant enzyme activities were determined in the erythrocyte lysates and membranes of type I and II alcoholics in order to clarify the effect of age-of-onset and the duration of the alcohol consumption on erythrocyte...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1125
更新日期:2005-01-01 00:00:00
abstract::Growth factors are biologically-active mediators that bind to specific receptors on target cells and regulate genes involved in cell growth, wound healing and regeneration. In the case of wound healing, a proper wound dressing is needed to cover the wound area, protect the damaged tissue, and if possible to activate c...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1121
更新日期:2004-11-01 00:00:00
abstract::Ovarian cancer is one of the most common female malignancies, and cisplatin-based chemotherapy is routinely used in locally advanced ovarian cancer patients. Acquired or de novo cisplatin resistance remains the barrier to patient survival, and the mechanisms of cisplatin resistance are still not well understood. In th...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3319
更新日期:2018-03-01 00:00:00
abstract::Cardiac hypertrophy is characterized by remodeling of the extracellular matrix (ECM). Integrins are cell-surface molecules that link the ECM to the cellular cytoskeleton where they play roles as signaling molecules and transducers of mechanical force. To clarify the possible roles of integrins in cardiac myocyte hyper...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.988
更新日期:2003-03-01 00:00:00
abstract::The study of the influence of the age of the animals (13 to 53 weeks) on the rate of ethanol metabolism in vivo and the total activity of liver alcohol dehydrogenase and microsomal ethanol oxidizing system showed a progressive decline with age. These effects were observed concomitantly with a diminution in the content...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290060103
更新日期:1988-01-01 00:00:00
abstract::Endothelial dysfunction is recognized as the initial detectable stage of cardiovascular disease, a serious complication of diabetes. In this study, we evaluated effects of myricetin on high glucose (HG)-elicited oxidative damage in human umbilical vein endothelial cells (HUVECs). The cells were pre-incubated with myri...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3442
更新日期:2020-01-01 00:00:00
abstract::Rat intestinal epithelial cells were isolated by EDTA-chelation, combined with gentle shaking (modified Weiser procedure) or with strong longitudinal vibration (Harrison/Webster procedure). Both methods yield large numbers of viable cells and are relatively easy to use. Electronmicroscopical and biochemical data indic...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020208
更新日期:1984-04-01 00:00:00
abstract::Nuclear, chromatin and microsomal fractions were isolated from hepatocytes prepared from rats injected with [32P]O4(2-) and killed subsequently at times between 1 and 48 h. Specific activities of the total phospholipids (PL) were determined for each subcellular fraction. The major points noted were the initial specifi...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290040409
更新日期:1986-10-01 00:00:00
abstract::The new glass ionomer dental cement, ChemFil, was found to be moderately cytotoxic when freshly mixed in vitro. This was assessed by a reduction in fibroblast and macrophage counts following 24 h exposure to the material, and by alterations in enzyme levels and enzyme staining kinetics relative to control cultures. Te...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290020112
更新日期:1984-01-01 00:00:00
abstract::Several polymorphisms in the CYP1A1 locus have been identified and their genotypes appear to exhibit population frequencies that depend on ethnicity. We studied two CYP1A1 polymorphic sites (position 4889 and 6235) in a group of 212 unrelated healthy individuals belonging to three different Mexican populations (106 Me...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1174
更新日期:2005-05-01 00:00:00
abstract::The effects of several novel monosaccharides upon thymidine incorporation into both normal and tumour cells were investigated. The monosaccharide 2-deoxy-3-[1-(R)-(ethoxycarbonyl)ethyl]- alpha-D-allo-pyranose had the most inhibitory effect on proliferation, with the (S)-enantiomer having less inhibitory effects. The c...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/(SICI)1099-0844(199712)15:4<243::AID-CBF74
更新日期:1997-12-01 00:00:00
abstract::The purpose of this study was to investigate the oxidative status in experimental hypothyroidism and the antioxidant effect of taurine supplementation. Forty male Sprague Dawley rats were randomly divided into four groups (group 1, control; group 2, control + taurine; group 3, propylthiouracil (PTU); group 4, PTU + ta...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.1198
更新日期:2006-03-01 00:00:00
abstract::The effect of citrinin on Ca2+ transport was studied in isolated kidney cortex and liver mitochondria, and baby hamster kidney cultured cells. The mycotoxin significantly inhibited the activity of 2-oxoglutarate and pyruvate dehydrogenases in both kidney cortex and liver mitochondria. Citrinin promoted a decrease in t...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290130110
更新日期:1995-03-01 00:00:00
abstract::The rat osteosarcoma cell line UMR-106-01 has an osteoblast-like phenotype. When grown in monolayer culture these cells transport inorganic phosphate and L-alanine via Na(+)-dependent transport systems. Exposure of these cells to a low phosphate medium for 4 h produced a 60-70 per cent increase in Na(+)-dependent phos...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.290110207
更新日期:1993-06-01 00:00:00
abstract::Epidermal growth factor plays a major role in breast cancer cell proliferation, survival, and metastasis. Quercetin, a bioactive flavonoid, is shown to exhibit anticarcinogenic effects against various cancers including breast cancer. Hence, the present study was designed to evaluate the effects of gold nanoparticles-c...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3266
更新日期:2017-06-01 00:00:00
abstract::Intrahepatic cholangiocarcinoma (ICC) is the second most common primary liver cancer, and thymidine phosphorylase (TP) is a regulator of angiogenesis. To investigate the biological activities of TP in ICC, we established human cholangiocarcinoma RBE cell lines overexpressing TP or silencing TP. Overexpression of TP en...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.3541
更新日期:2020-08-01 00:00:00
abstract::Incidence and mortality rates of cancer continue to increase greatly despite the improved diagnostic and therapeutic methods. Based on GLOBOCAN estimates, the numbers of new cancer cases reported in 2018 were ~18.1 million, while the numbers of cancer mortalities were ~9.6 million. It remains difficult to diagnose mos...
journal_title:Cell biochemistry and function
pub_type: 杂志文章,评审
doi:10.1002/cbf.3601
更新日期:2020-11-06 00:00:00
abstract::Liver-directed gene therapy has become a promising treatment for many liver diseases. In this study, we constructed a multi-functional targeting molecule, which maintains targeting, endosome-escaping, and DNA-binding abilities for gene delivery. Two single oligonucleotide chains of Melittin (M) were synthesized. The f...
journal_title:Cell biochemistry and function
pub_type: 杂志文章
doi:10.1002/cbf.2958
更新日期:2013-12-01 00:00:00