The assay of uridine diphosphoglucose dehydrogenase activity: discrimination from xanthine dehydrogenase activity.

Abstract:

:The biochemical and quantitative cytochemical assays of the activity of uridine diphosphoglucose dehydrogenase (UDPG-D) have produced perplexing results. It is now shown that the perplexity may be due to the possibility that the coenzyme (NAD) required for UDPG-D activity, may be acting as a substrate for a second dehydrogenase, namely xanthine dehydrogenase, which may utilize NAD as its substrate. The activity of UDPG-D can be distinguished selectively by the pH of its optimal activity and by decreasing the concentration of the coenzyme used in the assay.

journal_name

Cell Biochem Funct

authors

Mehdizadeh S,Bitensky L,Chayen J

doi

10.1002/cbf.290090207

subject

Has Abstract

pub_date

1991-04-01 00:00:00

pages

103-10

issue

2

eissn

0263-6484

issn

1099-0844

journal_volume

9

pub_type

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