Abstract:
OBJECTIVE:To explore the accurate Fas antigenic expression in transitional cell carcinoma (TCC) cells and and compare its synergistic modulation on adriamycin cytotoxicity with alpha-tumor necrosis factor (TNF-alpha). METHODS:The expression of Fas antigen in normal urothelium and TCC cell lines was measured by flow cytometry. The Fas/Fas ligand reaction-induced cytotoxic changes in tumor cells were evaluated by microculture MTT technique. The synergism efficacy of Fas-mediated apoptosis and TNF-alpha on adriamycin cytotoxicity of TCC cells was analyzed. The Fas/Fas ligand-induced apoptosis in tumor cells was studied by annexin-V apoptotic cell expression and DNA ladder fragmentation analysis. RESULTS:75% of TCC cell lines showed Fas antigen expression. The Fas expression was not influenced by in vitro growth density of tumor cells. Apoptosis of TCC cells was observed during 48 h of treatment after activation of the Fas/Fas ligand pathway. TNF-alpha had a higher cytotoxicity activity on TCC cells than Fas ligand (17 vs. 0.7%) while combination of both reagents had 30% increased synergistic cytotoxicity. The increasing rate of apoptotic body after 3 days of treatment was 74% in adriamycin, 32% in Fas ligand, 60% in TNF-alpha, 69% in Fas and adriamycin combination and Fas and TNF-alpha combination, 103% in combination of TNF-alpha and adriamycin, and 136% in combination of these three reagents individually. In the DNA ladder analysis, Fas ligand had a 1.5-fold increase of DNA fragmentation when compared with adriamycin while TNF-alpha had a 4.4-fold increase and triple combination had a 5.5-fold increase after 3 days of treatment. CONCLUSIONS:Although the Fas antigen expression is common in TCC cells and apoptosis can be activated by the Fas/Fas ligand pathway activation, the synergistic cytotoxicity of adriamycin by the Fas/Fas ligand pathway is lower than that of TNF-alpha.
journal_name
Urol Intjournal_title
Urologia internationalisauthors
Yu DS,Hsieh DS,Chen HI,Chang SYdoi
10.1159/000068772subject
Has Abstractpub_date
2003-01-01 00:00:00pages
161-6issue
3eissn
0042-1138issn
1423-0399pii
68772journal_volume
70pub_type
杂志文章abstract:PURPOSE:To assess our short-term experience with transurethral microwave thermotherapy (TUMT) for symptomatic benign prostatic hyperplasia (BPH). PATIENTS AND METHODS:From August 1993 through July 1994, in total 65 patients with symptomatic BPH were enrolled into this study. The patients' ages ranged from 56 to 95 yea...
journal_title:Urologia internationalis
pub_type: 临床试验,杂志文章
doi:10.1159/000064846
更新日期:2000-01-01 00:00:00
abstract:INTRODUCTION:To identify the risk factors, characteristics and prognosis of patients treated with docetaxel-based chemotherapy for peritoneal carcinomatosis due to metastatic castrate-resistant prostate cancer (mCRPC). METHODS:We retrospectively reviewed our series of mCRPC patients with peritoneal metastases treated ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000354396
更新日期:2014-01-01 00:00:00
abstract::A case of malignant peripheral nerve sheath tumor of the bladder in a 57-year-old man with multiple neurofibromatosis type 1 is presented. The patient had a recent history of a transurethrally resected bladder ganglioneuroma. A probable histogenetic association between these two extremely rare neoplasms is proposed. ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000065568
更新日期:2002-01-01 00:00:00
abstract::35 women, referred for simple total hysterectomy, were evaluated pre- and postoperatively for bladder suspension and urodynamic changes. Although all patients denied urological problems, more than half of the patients had some degree of stress and/or urge incontinence. 40% had radiological signs of bladder suspension ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000281085
更新日期:1985-01-01 00:00:00
abstract:AIM:To analyze demographics, perioperative mortality and overall survival of radical cystectomy (RC) in patients aged 70+ years in Austria in a nation-wide registry cohort. PATIENTS AND METHODS:All patients >69 years who underwent RC in public hospitals (covering >95% of all surgical procedures) in Austria between 199...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000316100
更新日期:2010-01-01 00:00:00
abstract:INTRODUCTION:Open radical cystectomy (ORC) is currently the standard treatment for muscle-invasive bladder cancer (MIBC) without metastasis, while many patients with MIBC are not always appropriate candidates due to multiple comorbidities. To evaluate the bladder-preservation strategy, we compared the results with thos...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000477912
更新日期:2017-01-01 00:00:00
abstract::Cystinuria is a rare cause of renal calculi, whose management presents a complex problem mainly due to the hardness and high recurrence rate of cystine stones. During the period 1987-1991, 28 established cases of cystine calculi were treated by extracorporeal shock wave lithotripsy (ESWL) and percutaneous nephrolithot...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282836
更新日期:1996-01-01 00:00:00
abstract::The ability of three compounds, all similar in chemical structure to citric acid, to decrease calcium crystalluria has been measured. The measurements were made in normal human urine at 37 degrees C and compared with the crystal-decreasing power of citric acid when measured in the same way and in the same urine sample...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282875
更新日期:1996-01-01 00:00:00
abstract:OBJECTIVES:Since little investigation has been undertaken to determine if alpha1-blockers should be given continuously to sustain their efficacy, we conducted a pilot study to determine symptom change following discontinuation of tamsulosin after an initial improvement in symptoms in men with lower urinary tract sympto...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000092052
更新日期:2006-01-01 00:00:00
abstract::119 patients with stage-IV renal cell carcinoma were treated using immunotherapy with autologous tumor vaccine. The immunization was carried out at monthly intervals, the patients were restaged every 3 months using X-ray, ultrasound scanning or computed tomography as well as bone scintigraphy in the follow-up. The pat...
journal_title:Urologia internationalis
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1159/000281439
更新日期:1989-01-01 00:00:00
abstract::We present a 50-year-old male with renal cell carcinoma and hypercalcemia presenting as acute pancreatitis. The sudden onset of epigastralgia, acute pancreatitis and hypercalcemia with raised parathyroid-like peptide levels in this patient could not be explained except for renal cell carcinoma. This is the first repor...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000048452
更新日期:2002-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the management of urolithiasis in children since the development of extracorporeal shock-wave lithotripsy (ESWL). METHODS:Between 1988 and 1994, 37 children, aged from 2 to 15 years (mean 10), with upper tract urolithiasis were evaluated and treated. Lithogenic metabolic disorders or anomalies of...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282882
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:To determine association between urine pH and OAB symptoms and to determine if urinary alkalization improves overactive bladder (OAB) symptoms. METHODS:659 patients with OAB were enrolled in this study between June 2012 and May 2014. 329 patients (group 1) were included in the final analysis. 201 adults wer...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000375322
更新日期:2015-01-01 00:00:00
abstract::A total of 51 consecutive female patients with genuine stress incontinence who underwent a Burch or Stamey operation were clinically and urodynamically evaluated preoperatively at least 8 months postoperatively. Our study group consisted of 27 women who underwent the Burch colposuspension and 24 who had the Stamey end...
journal_title:Urologia internationalis
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000282803
更新日期:1996-01-01 00:00:00
abstract::Two cases of colorenocutaneous fistula due to genitourinary tuberculosis are presented. They were successfully managed by single-stage surgery (nephroureterectomy, fistulectomy, and primary repair of the colon) and antitubercular treatment. The literature is briefly reviewed. ...
journal_title:Urologia internationalis
pub_type: 杂志文章,评审
doi:10.1159/000282568
更新日期:1994-01-01 00:00:00
abstract::To help bolster penile tension mechanically, we have tried implantation of silicone penile prostheses varying in type. Our simple and apparently successful procedures for organic impotence with the implantation of intrapenile silicone prostheses may well deserve recommendation in view of spinal impairment therapeutica...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000279981
更新日期:1975-01-01 00:00:00
abstract:INTRODUCTION:Splenectomy had been previously performed in ABO-incompatible kidney transplantation to reduce the B cell pool. However, studies have shown that patients undergoing splenectomy may have a lifelong susceptibility to infection and mortality. Splenectomy may affect the incidence of cytomegalovirus (CMV) disea...
journal_title:Urologia internationalis
pub_type:
doi:10.1159/000504862
更新日期:2020-01-01 00:00:00
abstract::Amputation of the penis is by no means the quickest and least invasive of the surgical methods of treating penis carcinoma. Radiation therapy has the advantage of preserving the integrity of the penis and does not render a later operation of the penis impossible. Regional lymph node metastases should be removed surgic...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000280899
更新日期:1983-01-01 00:00:00
abstract::Multiple oral doses of alpha-difluoromethyl-ornithine (alpha-DFMO), 18-24 g/day for up to 2 months, were administered to 2 patients with invasive and metastatic carcinoma of the bladder and to 3 patients with metastatic renal cancer in an open study. The moderate antigrowth effect of alpha-DFMO in these patients was c...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000280403
更新日期:1981-01-01 00:00:00
abstract::In order to determine an optimum mode for systemic administration of recombinant interleukin 2 (rIL2), the effects of rIL2 on lymphocyte-mediated cytotoxicity against renal carcinoma cells were studied in vitro. Augmentation of the cytotoxicity by rIL2 was dose- and time-dependent. The optimum dose of rIL2 was 100-500...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282272
更新日期:1991-01-01 00:00:00
abstract::Cyclic dysmenorrhea, unilateral abdominopelvic mass, acute urinary retention and renal agenesis ipsilateral to the pelvic mass in a young woman suggest uterus didelphys with unilaterally imperforate vagina. ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282018
更新日期:1987-01-01 00:00:00
abstract::Patients with prostatic carcinoma were randomised to treatment with surgical castration (n = 47) or oral oestrogen therapy (n = 53). The oestrogen-treated patients received oral ethinyl oestradiol daily, 1 mg for the first 2 weeks followed by 150 micrograms. In addition, oestrogen was given intramuscularly as polyoest...
journal_title:Urologia internationalis
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000282639
更新日期:1994-01-01 00:00:00
abstract::The effect of vinblastine sulfate on the acceptance and growth of a very malignant, human adenocarcinoma of the kidney (RCCI) on nude mice was investigated. Without any treatment, this tumor has an acceptance rate of 100%. Subcutaneously transplanted pieces of tumor, measuring 3 X 3 X 1 mm, commence to grow rapidly 1 ...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000280404
更新日期:1981-01-01 00:00:00
abstract::Adherence of human prostate carcinoma (PC 3, DU 145), bladder (647 V, J 82) carcinoma and melanoma (RPMI-8252) cell lines to plastic and bovine endothelial monolayers (BEM) was tested in the presence of 0.5, 1, 5, 10, 20 IU of low-molecular-weight heparin (LMWH), unfractionated heparin (UFH) or saline as a control. Cu...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000282848
更新日期:1996-01-01 00:00:00
abstract:OBJECTIVE:The study aimed to document the types of meatal occlusive disease (MOD) in adult males in terms of its clinical presentation, management and prognosis. MATERIAL AND METHODS:Eighty-six adult males with MOD were assessed during the study period. The patients were divided into Group 1, having meatal opening tha...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000446221
更新日期:2016-01-01 00:00:00
abstract:INTRODUCTION:This study assesses the differences in the presentations, complications and metabolic abnormalities of children with renal calyceal microlithiasis (RCM) and overt urolithiasis in different pediatric ages. MATERIALS AND METHODS:A total of 465 children with urolithiasis were investigated retrospectively. Pa...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000441126
更新日期:2016-01-01 00:00:00
abstract:INTRODUCTION:The treatment of T1G3 bladder cancer is still a controversial issue. Nowadays, intravesical bacillus Calmette-Guérin (BCG) instillation is considered to be the treatment of choice for patients with high-grade superficial bladder tumour after transurethral resection of all visible tumour. The aim of this re...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000066121
更新日期:2002-01-01 00:00:00
abstract::The introduction in 1982 of vasoactive agents for intracavernous injection represents a milestone in the diagnosis and treatment of erectile dysfunction. Two preparations, the single drug papaverine hydrochloride and the combination of phentolamine mesylate and papaverine hydrochloride, hold great promise. In the last...
journal_title:Urologia internationalis
pub_type: 杂志文章,评审
doi:10.1159/000281308
更新日期:1988-01-01 00:00:00
abstract:INTRODUCTION AND OBJECTIVE:When feasible, the treatment for all-invasive bladder cancer is radical cystectomy. The aim of the present study was to analyze the prognostic difference, disease-specific survival rate, of muscle-invasive transitional cell cancer of the bladder (TCCB) for progressive invasive TCCB. PATIENTS...
journal_title:Urologia internationalis
pub_type: 杂志文章,多中心研究
doi:10.1159/000107950
更新日期:2007-01-01 00:00:00
abstract:INTRODUCTION:Renal cell carcinoma (RCC) reveals a tendency towards venous invasion in its advanced stages, making clinical management challenging. Survival may be improved following surgery, but is less clear if it applies to patients with metastatic disease at diagnosis. MATERIALS AND METHODS:Review of clinical files...
journal_title:Urologia internationalis
pub_type: 杂志文章
doi:10.1159/000493510
更新日期:2018-01-01 00:00:00