Abstract:
:Conjugation of dextran-coated superparamagnetic iron oxide (SPIO) particles with transactivator protein (Tat)-peptide and fluorescein isothiocyanate (FITC) allows cells to readily uptake SPIO particles. This makes possible high-resolution, real-time imaging of these cells by magnetic resonance imaging (MRI). First, we need to understand how various subpopulations take up and maintain SPIO particles. In this report, we have focused on differences in T cells, B cells, and macrophages with respect to cross-linked (CL)-SPIO Tat-FITC particle uptake over 72 hours. We have found that cells quickly take up the particles and that the bead loss that does occur is not related to cell death or apoptosis. In contrast with reports in the literature, we have observed migration of the Tat-peptide conjugates primarily to the cytoplasm rather than the nucleus.
journal_name
Transplantationjournal_title
Transplantationauthors
Kaufman CL,Williams M,Ryle LM,Smith TL,Tanner M,Ho Cdoi
10.1097/01.TP.0000090164.42732.47subject
Has Abstractpub_date
2003-10-15 00:00:00pages
1043-6issue
7eissn
0041-1337issn
1534-6080journal_volume
76pub_type
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