Abstract:
:Natural anti-carbohydrate antibodies are central to hyperacute rejection in ABO-incompatible allotransplantation and in discordant xenotransplantation. ABO-incompatible rejection has been inhibited successfully using intravenous soluble carbohydrates as antibody inhibitors. The approach has been less successful previously in pig to primate xenotransplantation, where the necessary concentrations of a partial inhibitor (Gal alpha 1-6Glc) proved highly toxic. In this study, we have identified more effective inhibitors of the dominant human anti-pig antibodies that bind to the pentasaccharide Gal alpha 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-. The inhibitors are the terminal disaccharide (Gal alpha 1-3Gal) and terminal trisaccharide (Gal alpha 1-3Gal beta 1-4GlcNAc) of the target pentasaccharide. Twelve sera (3 from each ABO blood group) were tested in 3 different assays: lymphocytotoxic, lymphocyte flow cytometry, and solid-phase antigen ELISA. Fifty percent to 75% inhibition of human IgG and IgM was achieved using the disaccharide and trisaccharide inhibitors in the range of 10-50 mM. Disaccharide (70 mM) was used to inhibit hyperacute thrombosis in pig kidneys perfused for 40 min with heparinized human AB whole blood. The disaccharide completely inhibited red cell occlusion of glomerular but not of intertubular capillaries, although there was residual platelet thrombus in glomeruli. Disaccharide and trisaccharide can, therefore, be used in concentrations shown for other carbohydrate inhibitors to be nontoxic, for inhibition of hyperacute pig-to-human xenograft rejection. The inhibition is incomplete, however, and other antigen specificities and other rejection mechanisms are likely to be involved.
journal_name
Transplantationjournal_title
Transplantationauthors
Cairns T,Lee J,Goldberg L,Cook T,Simpson P,Spackman D,Palmer A,Taube Dsubject
Has Abstractpub_date
1995-12-15 00:00:00pages
1202-7issue
11eissn
0041-1337issn
1534-6080journal_volume
60pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:Dendritic cells (DC) can exert powerful immune stimulatory as well as regulatory functions and are therefore important tools for therapeutic strategies. Dexamethasone (Dex) was previously shown to inhibit DC maturation and to induce regulatory properties both in vitro and in vivo. Here, we investigated the i...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31818a6682
更新日期:2008-11-15 00:00:00
abstract::The immunological events mediated by, and antigen specificity of, allograft-bound lymphocytes (ABLs) are poorly understood. To further define the role of antibody-mediated rejection, a rejected allograft from a patient with primary anti-TBM disease was sterilely minced and pressed through a microscreen. The ABLs were ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198602000-00008
更新日期:1986-02-01 00:00:00
abstract:BACKGROUND:Primary hyperoxaluria type-1 (PH1) is an autosomal recessive disease characterized by excessive oxalate production by hepatocytes caused by the deficiency of peroxisomal alanine-glyoxylate aminotransferase (AGT) activity. Persistent hyperoxaluria causes nephrocalcinosis and urolithiasis, leading to renal fai...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31816de49e
更新日期:2008-05-15 00:00:00
abstract::Since June 1979, percutaneous transluminal angioplasty (PTA) has been the procedure of choice for renal transplant artery stenosis (RTAS) at the Hospital of the University of Pennsylvania. Of 241 renal allograft recipients, 17 (7%) when studied by arteriogram because of suspected RTAS proved to have significant stenos...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198212000-00005
更新日期:1982-12-01 00:00:00
abstract::Organ shortage in liver transplantation has led the transplant community to expand the donor organ pool and reduce waiting list mortality by using so-called marginal donor or extended criteria donor (ECD) livers. The exact impact of these criteria on graft function and recipient survival are still under investigation....
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e318186ad7a
更新日期:2008-10-15 00:00:00
abstract:BACKGROUND:Immunosuppressive drugs that target T cells are useful for prolonging allograft survival. The anti-CD3 immunotoxin FN18-CRM9 has been shown to effectively prolong renal allograft survival in a rhesus monkey model of transplantation. However, immunotoxin-treated monkeys showed increased levels of inflammatory...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199909150-00016
更新日期:1999-09-15 00:00:00
abstract:BACKGROUND:It is well known that kidney transplant recipients with preformed lymphocytotoxic antibodies against HLA antigens have an increased graft rejection rate. However, the individual contribution of anti-HLA class I and class II antibodies to this phenomenon is poorly understood. We investigated the clinical rele...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200204270-00014
更新日期:2002-04-27 00:00:00
abstract::Limited recovery of contractile function and loss of coronary reactivity have been observed following prolonged hypothermic storage and transplantation of the heart. Since lipid peroxidation has a significant role in these deficits, we investigated the cardioprotective effects of a 21-aminosteroid. U74389G, 3 mg/kg i....
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199604150-00007
更新日期:1996-04-15 00:00:00
abstract::Endothelial injury plays a central role in the pathophysiologic mechanisms underlying cardiac allograft vasculopathy (CAV). Although the accelerated course of CAV and its localization to the allograft support an important role for the alloimmune response, there is considerable evidence implicating lipoprotein abnormal...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/01.TP.0000080981.90718.EB
更新日期:2003-09-27 00:00:00
abstract:BACKGROUND:Although chronic kidney disease (CKD) is still a common complication, the prognosis of kidney function after liver transplantation (LT) is not well known. Moreover, kidney function after LT in children with renal involvement is unknown. METHODS:We retrospectively analyzed patients aged <20 years who underwe...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002548
更新日期:2019-08-01 00:00:00
abstract:BACKGROUND:Survival following liver transplantation for hepatitis C virus (HCV) is significantly poorer than for liver transplants performed for other causes of chronic liver disease. The factors responsible for the inferior outcome in HCV+ recipients, and whether they differ from factors associated with survival in HC...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000130468.36131.0d
更新日期:2004-06-27 00:00:00
abstract:BACKGROUND:Alcoholic liver disease has emerged as a leading indication for hepatic transplantation, although it is a controversial use of resources. We aimed to examine all aspects of liver transplantation associated with alcohol abuse. METHODS:Retrospective cohort analysis of 123 alcoholic patients with a median of 7...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200108270-00010
更新日期:2001-08-27 00:00:00
abstract::KM2210, a conjugate of estradiol and chlorambucil (CBL), which was originally developed as an anti-breast cancer agent, inhibits proliferative response of human mononuclear cells to alloantigens in mixed lymphocyte culture in a dose-dependent manner, but has no effect on their response to phytohemagglutinin. Neither e...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199210000-00022
更新日期:1992-10-01 00:00:00
abstract::In order to study further whether a relationship exists between the extent of ischemia-preservation-reperfusion injury (IPRI) and acute rejection (AR) events in liver allografts, we retrospectively reviewed 213 consecutive cyclosporine-treated patients who received their first liver allograft between 1/1/93 and 12/31/...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199509270-00006
更新日期:1995-09-27 00:00:00
abstract::Patients awaiting a renal transplant were given 1 HLA-DR antigen-matched blood in order to compare the sensitization and graft outcome of these patients with those of control patients, who had been given random blood before transplantation. No differences in sensitization, measured by the formation of HLA antibodies, ...
journal_title:Transplantation
pub_type: 临床试验,杂志文章
doi:
更新日期:1994-10-15 00:00:00
abstract:BACKGROUND:Pancreatic islets are avascular immediately after transplantation and depend on revascularization. Recently, the authors found decreased vascular density in mouse islets 1 month after implantation into nondiabetic recipients. This study investigated possible differences in revascularization between islets im...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000052592.92966.FE
更新日期:2003-03-15 00:00:00
abstract:BACKGROUND:We recently described a new approach that enables the generation of human/mouse chimera by adoptive transfer of human peripheral blood mononuclear cells into lethally irradiated normal strains of mice or rats, radioprotected with bone marrow from donors with severe combined immune deficiency. In such human/m...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199704270-00018
更新日期:1997-04-27 00:00:00
abstract::Cyclosporine (CsA) is an immunosuppressive drug requiring dose individualization and regular control due to its highly variable pharmacokinetics. Since gastroparesis may influence the absorption of CsA, a randomized cross-over study was performed to assess the pharmacokinetics and tolerability of a novel microemulsion...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00007890-199608270-00006
更新日期:1996-08-27 00:00:00
abstract:BACKGROUND:Allografts are occasionally accepted in the absence of immunosuppression. Because naturally occurring CD4(+)CD25(+) regulatory T cells (natural CD25(+) Treg cells) have been shown to inhibit allograft rejection, we investigated their influence on the outcome of allografts in nonimmunosuppressed mouse recipie...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000155179.61445.78
更新日期:2005-03-27 00:00:00
abstract:BACKGROUND:The detection of acute rejection in heart transplantation remains an important feature of transplant management, especially in the early phase. Frequent surveillance with endomyocardial biopsy is necessary, even though it is an invasive procedure and carries a certain risk. Hence, noninvasive biomarkers able...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181919f5d
更新日期:2009-01-27 00:00:00
abstract::Tolerance induction by CD45RB monoclonal antibody (mAb) in murine allograft models is associated with an alteration in the CD45RBlo/CD45RBhi T-cell ratio in favor of CD45RBlo T cells, which can function as regulatory cells and promote tolerance. It has been proposed that inversion of the CD45RBhi/CD45RBlo normal T-cel...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000072373.77323.D4
更新日期:2003-07-27 00:00:00
abstract:BACKGROUND:We reviewed the incidence of and risk factors for venous thromboembolic complications in our population of kidney (KTx) and simultaneous kidney-pancreas transplant (SPK) recipients. METHODS:Information was collected retrospectively from a database on 1833 KTx and 276 SPK recipients who underwent transplant ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199801270-00015
更新日期:1998-01-27 00:00:00
abstract::A murine IgG1 antibody specific for the IL-2-binding site on the human lymphocyte IL-2 receptor beta chain (CD25) was evaluated in 11 patients who developed acute graft-versus-host disease following allogeneic marrow transplantation. All patients had received cyclosporine and methotrexate for prophylaxis of GVHD, eith...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199007000-00010
更新日期:1990-07-01 00:00:00
abstract:BACKGROUND:NKp46 expression in natural killer (NK) cells has recently been shown to affect the responsiveness to antiviral treatment in hepatitis C virus (HCV)-infected patients. However, the density of NKp46 on intrahepatic NK cells is remarkably higher than that on peripherally circulating NK cells, whereas the bioph...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001031
更新日期:2016-02-01 00:00:00
abstract:BACKGROUND:In the present study, we investigated whether the soluble form of CD30 (sCD30), a marker for T helper 2-type cytokine-producing T cells, is increased in sera of potential kidney graft recipients. We also investigated whether the pretransplantation serum sCD30 content is related to kidney graft survival. MET...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200201150-00002
更新日期:2002-01-15 00:00:00
abstract:BACKGROUND:Long-term use of immunosuppressants is associated with significant morbidity and mortality in transplant recipients. A simple whole blood assay that has U.S. Food and Drug Administration clearance directly assesses the net state of immune function of allograft recipients for better individualization of thera...
journal_title:Transplantation
pub_type: 杂志文章,meta分析
doi:10.1097/01.tp.0000234837.02126.70
更新日期:2006-09-15 00:00:00
abstract::We describe a substrain of BB rats, BB/Pfd, characterized by a loss of autoimmune potential soon after onset of diabetes. When fresh syngeneic (BB/Pfd) islets (6 islets/g body weight) were transplanted under the kidney capsule of diabetic BB/Pfd rats 1-2 weeks after diabetes onset (n = 14), no recurrence of diabetes o...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1994-08-15 00:00:00
abstract:BACKGROUND:A new vascularized concordant xenotransplant model using the Chinese hamster as donor and mouse as recipient species is reported. This model takes advantage of the wealth of informative immune reagents and knockout and transgenic backgrounds available for the mouse. METHODS:Heterotopic auxillary cardiac tra...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199906150-00016
更新日期:1999-06-15 00:00:00
abstract::Cold-storage preservation of the canine pancreas prior to islet isolation has previously been noted to reduce the intrasplenic islet autograft success rate; but the mechanism of this deleterious effect has not been determined. We undertook a study in both outbred dogs and Lewis (RT1-1) rats to determine the influence ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198901000-00007
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND:We hypothesized that solitary pancreas transplants could be performed successfully even in the presence of poor HLA matching if an aggressive approach were taken with regard to immunosuppressive protocol and the performance of allograft biopsy. METHODS:Seven pancreas-after-kidney transplants and seven pancr...
journal_title:Transplantation
pub_type: 杂志文章,多中心研究
doi:10.1097/00007890-200007270-00028
更新日期:2000-07-27 00:00:00