Anoxia reduces depolarization induced calcium uptake in the rat hippocampal slice.

Abstract:

:Veratridine-induced depolarization caused a large increase in Ca uptake in the rat hippocampal slice (30.2 vs. 9.0 nM/mg dry weight). This uptake was reduced to 18.4 nM/mg when veratridine was combined with anoxia. When compared with veratridine exposure alone, the combination of anoxia and veratridine increased intracellular Na (460 vs. 380 microM/g), decreased intracellular K (30 vs. 40 microM/g) and decreased ATP levels (0.1 vs. 0.8 nM/mg). The changes in Na, K, and ATP should enhance net Ca uptake, yet Ca uptake was reduced. This suggests an effect of anoxia to block Ca channels. In summary anoxia attenuates depolarization-induced Ca uptake. This may represent a mechanism by which neurons are partially protected against anoxic damage which could be more severe if depolarization-induced Ca uptake was not limited.

journal_name

Brain Res

journal_title

Brain research

authors

Kass IS,Abramowicz AE,Cottrell JE,Amorim P,Chambers G

doi

10.1016/0006-8993(94)91547-4

subject

Has Abstract

pub_date

1994-01-07 00:00:00

pages

262-6

issue

1-2

eissn

0006-8993

issn

1872-6240

pii

0006-8993(94)91547-4

journal_volume

633

pub_type

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