Abstract：
:Deformational loading represents a primary component of the chondrocyte physical environment in vivo. This review summarizes our experience with physiologic deformational loading of chondrocyte-seeded agarose hydrogels to promote development of cartilage constructs having mechanical properties matching that of the parent calf tissue, which has a Young's modulus E(Y) = 277 kPa and unconfined dynamic modulus at 1 Hz G* = 7 MPa. Over an 8-week culture period, cartilage-like properties have been achieved for 60 x 10(6) cells/ml seeding density agarose constructs, with E(Y) = 186 kPa, G* = 1.64 MPa. For these constructs, the GAG content reached 1.74% ww and collagen content 2.64% ww compared to 2.4% ww and 21.5% ww for the parent tissue, respectively. Issues regarding the deformational loading protocol, cell-seeding density, nutrient supply, growth factor addition, and construct mechanical characterization are discussed. In anticipation of cartilage repair studies, we also describe early efforts to engineer cylindrical and anatomically shaped bilayered constructs of agarose hydrogel and bone (i.e., osteochondral constructs). The presence of a bony substrate may facilitate integration upon implantation. These efforts will provide an underlying framework from which a functional tissue-engineering approach, as described by Butler and coworkers (2000), may be applied to general cell-scaffold systems adopted for cartilage tissue engineering.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Hung CT,Mauck RL,Wang CC,Lima EG,Ateshian GAdoi
10.1023/b:abme.0000007789.99565.42subject
Has Abstractpub_date
2004-01-01 00:00:00pages
35-49issue
1eissn
0090-6964issn
1573-9686journal_volume
32pub_type
杂志文章,评审abstract：:Pathologic vascular adaptation following local injury is the primary driver for accelerated intimal hyperplasia and an occlusive phenotype. Smooth muscle cell (SMC) proliferation within the wall, and migration into the developing intima, is a major component of this remodeling response. The primary objective in the cu...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1725-0
更新日期：2017-03-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
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更新日期：2015-03-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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更新日期：2020-06-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 临床试验,杂志文章
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更新日期：2014-01-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.43
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-017-1937-y
更新日期：2018-01-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-012-0703-4
更新日期：2013-04-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-008-9511-2
更新日期：2008-08-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-019-02290-5
更新日期：2019-11-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0023-5
更新日期：2010-08-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-009-9650-0
更新日期：2009-04-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1289458
更新日期：2000-07-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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更新日期：2011-02-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0242-9
更新日期：2011-05-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-004-7820-7
更新日期：2004-12-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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更新日期：2004-06-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368017
更新日期：1988-01-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02367084
更新日期：1994-09-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02771001
更新日期：1996-01-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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更新日期：2011-07-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-9964-y
更新日期：2010-07-01 00:00:00
