Abstract：
:Ever-increasing demand for bone grafts necessitates the realization of clinical implementation of bone tissue engineered constructs. The predominant hurdle to implementation remains to be securing FDA approval, based on the lack of viable methods for the rigorous monitoring of said constructs. The study presented herein details a method for such monitoring based on the shifting metabolism of mesenchymal stem cells (MSCs) as they differentiate into osteoblasts. To that end, rat MSCs seeded on 85% porous spunbonded poly(L-lactic acid) scaffolds were cultured in flow perfusion bioreactors with baseline or osteoinductive media, and levels of key physio-metabolic markers (oxygen, glucose, osteoprotegerin, and osteocalcin) were monitored throughout culture. Comparison of these non-destructively obtained values and current standard destructive analyses demonstrated key trends useful for the concurrent real-time monitoring of construct cellularity and maturation. Principle among these is the elucidation of the ratio of the rates of oxygen uptake to glucose consumption as a powerful quality marker. This ratio, supported on a physiological basis, has been shown herein to be reliable in the determination of both construct maturation (defined as osteoblastic differentiation and accompanying mineralization) and construct cellularity. Supplementary monitoring of OPG and OCN are shown to provide further validation of such metrics.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Simmons AD,Sikavitsas VIdoi
10.1007/s10439-017-1937-ysubject
Has Abstractpub_date
2018-01-01 00:00:00pages
37-47issue
1eissn
0090-6964issn
1573-9686pii
10.1007/s10439-017-1937-yjournal_volume
46pub_type
杂志文章abstract：:The effect of stent design on wall shear stress (WSS) and oscillatory shear index (OSI) was studied in vitro using time-resolved digital particle image velocimetry (DPIV). Four drug-eluting stents [XIENCE V (Abbott Vascular), TAXUS Liberté (Boston Scientific), Endeavor (Medtronic), and Cypher (J&J Cordis)] and a bare-...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-009-9697-y
更新日期：2009-07-01 00:00:00
abstract：:This study was undertaken to investigate the possible origin of some cutaneously recorded higher frequency electrogastrographic signals. Computer modeling of gastric electrical uncoupling was performed using previously described conoidal dipole model. Cutaneous electrogastrograms were simulated after uncoupling was in...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:Coronary artery bypass graft (CABG) failure has been linked to graft hemodynamics, in particular wall shear stress. This study characterizes the morphology, geometry and wall shear stress patterns in human CABGs. The intimal thickness (IT) in 49 human saphenous vein CABGs was measured by digital light microscopy. The ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-005-1732-z
更新日期：2005-03-01 00:00:00
abstract：:The purpose of the study was to examine the effects of arterial coculture conditions on the transport properties of several in vitro endothelial cell (EC)-smooth muscle cell (SMC)-porous filter constructs in which SMC were grown to confluence first and then EC were inoculated. This order of culturing simulates the env...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-015-1421-5
更新日期：2016-05-01 00:00:00
abstract：:Perfusion bioreactors are known to exert shear stresses on cultured cells, leading to cell differentiation and enhanced extracellular matrix deposition on scaffolds. The influence of the scaffold's porous microstructure is investigated for a polycaprolactone (PCL) scaffold with a regular microarchitecture and a silk f...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-014-0981-0
更新日期：2014-05-01 00:00:00
abstract：:We compare the influence of time-frequency methods on analysis of time-varying renal autoregulation properties. Particularly, we examine if detection probabilities are similar for amplitude and frequency modulation for a modulated simulation signal among five time-frequency approaches, and if time-varying changes in s...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-012-0625-1
更新日期：2013-01-01 00:00:00
abstract：:Pulmonary arterial hypertension (PAH) is caused by extensive pulmonary vascular remodeling that increases right ventricular (RV) afterload and leads to RV failure. PAH predominantly affects women; paradoxically, female PAH patients have better outcomes than men. The roles of estrogen in PAH remain controversial, which...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1716-1
更新日期：2017-03-01 00:00:00
abstract：:The aim of this study was to establish in vitro bench-tests of left atrial appendage occlusion (LAAo) devices regarding tug force, radial force and sealing capacity. Two LAAo devices, namely the WATCHMAN™ and the Occlutech®, of three different sizes underwent testing in novel dedicated in vitro setups. Radial force wa...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-018-2057-z
更新日期：2018-09-01 00:00:00
abstract：:Some investigators have attempted to estimate the Reynolds shear stress on the basis of a single component of velocity. The purpose of this investigation is to determine the validity of such estimates in a complex flow field, such as occurs in the cardiovascular system in the region of the aortic valve. Turbulent velo...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02371247
更新日期：1985-01-01 00:00:00
abstract：:Gas bubble motion in a blood vessel causes temporal and spatial gradients of shear stress at the cell surface lining the vessel wall as the bubble approaches the cell, moves over it and passes it by. Rapid reversals occur in the sign of the shear stress imparted to the cell surface during this motion. These may result...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0120-5
更新日期：2010-12-01 00:00:00
abstract：:The left-right asymmetry in the potential amplitude on the scalp was studied in poststroke patients by using flash visual evoked potential (VEP) and a numerical two-dimensional model of the head. The left-right asymmetry of the VEP was measured in three patients after thrombosis, in one after hemorrhage, and in one he...
journal_title：Annals of biomedical engineering
pub_type： 临床试验,杂志文章
doi：10.1007/BF02770997
更新日期：1996-01-01 00:00:00
abstract：:We propose a multiphysical mathematical model by fully coupling lipid deposition, monocytes/macrophages recruitment and angiogenesis to investigate the pathophysiological responses of an atherosclerotic plaque to the dynamic changes in the microenvironment. The time evolutions of cellular (endothelial cells, macrophag...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-018-02173-1
更新日期：2019-02-01 00:00:00
abstract：:Vibration under shear flow causes the reduction of flow resistance for shear-thinning fluids. The present study investigates the effect of vibration on the flow resistance of a nonaggregating red blood cell (RBC) suspension with a newly designed pressure-scanning capillary viscometer (PSCV). The PSCV was originally de...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1603751
更新日期：2003-10-01 00:00:00
abstract：:High density particle image velocimetry, with spatial resolution of O(1 mm), was used to measure the effect of occluder rebound on the flow field near a Bjork-Shiley Monostrut tilting-disk mitral valve. The ability to measure two velocity components over an entire plane simultaneously provides a very different insight...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.281
更新日期：2000-04-01 00:00:00
abstract：BACKGROUND:This work explores for the first time the effects of temperature increments on the development of high shear stresses between plaque and arterial wall due to their different dilatational properties. Data from the literature report febrile reactions prior to myocardial infarction in patients with normal coron...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-007-9397-4
更新日期：2008-01-01 00:00:00
abstract：:Extraction of the cardiac surfaces of interest from multi-detector computed tomographic (MDCT) data is a pre-requisite step for cardiac analysis, as well as for image guidance procedures. Most of the existing methods need manual corrections, which is time-consuming. We present a fully automatic segmentation technique ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10439-015-1422-4
更新日期：2016-05-01 00:00:00
abstract：:The nonlinear diffusion-reaction equations describing the nonequilibrium transport of CO2 through flat layers of complex but homogeneous aqueous media were solved by an approximate analytical method called the "Combined Damköhler Number" (CDN) technique. Unlike other approximate analytical solutions, the CDN technique...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02484468
更新日期：1986-01-01 00:00:00
abstract：:When cells and the matrix of a tissue remodel, the mechanical properties of the tissue do change. The mechanical properties are expressed by constitutive equations. In this article the remodeling of the constitutive equation of the pulmonary artery is studied. The remodeling was induced in a rat breathing a gas whose ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1380417
更新日期：2001-01-01 00:00:00
abstract：:New porous implant designs made possible by additive manufacturing allow for increased osseointegration, potentially improving implant performance and longevity for patients that require massive bone implants. The aim of this study was to evaluate how implantation and the strain distribution in the implant affect the ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-019-02369-z
更新日期：2020-01-01 00:00:00
abstract：:This project was undertaken to establish if the integral circulation had any effects upon the mechanical behavior of bone. The influence of blood flow in the rabbit tibia on the strain induced at the bone surface when under load was assessed. Recordings were taken from three adult and three immature New Zealand White ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02390377
更新日期：1994-03-01 00:00:00
abstract：:The optimal means of electrically stimulating a skeletal muscle to contract around a fluid-filled pouch (i.e., a skeletal muscle ventricle [SMV]) has not been determined. A SMV was made from the latissimus dorsi muscle in five dogs and the rectus abdominis muscle in five dogs, and each SMV was electrically stimulated ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02364611
更新日期：1990-01-01 00:00:00
abstract：:Deformational loading represents a primary component of the chondrocyte physical environment in vivo. This review summarizes our experience with physiologic deformational loading of chondrocyte-seeded agarose hydrogels to promote development of cartilage constructs having mechanical properties matching that of the par...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1023/b:abme.0000007789.99565.42
更新日期：2004-01-01 00:00:00
abstract：:Phospholipids have been identified by thin-layer chromatography in appreciable quantities in pericardial fluid taken from 12 dogs and found to include sphingomyelin and phosphatidylcholines, -ethanolamines, -inositols and -serines--the cholines predominating. The extracts, the synthetic surfactants and a mixture of sy...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02584258
更新日期：1985-01-01 00:00:00
abstract：:Radiation applied for therapy and diagnostics damages molecular/atomic couples of biotissues, bones being among them. As a result, electron peculiarities and mechanical behavior of the latter are altered. Correlation between strength of the irradiated bone and its electron features explored due to photo- and exoelectr...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1114/1.1424911
更新日期：2001-12-01 00:00:00
abstract：:The traditional auscultatory technique and current methodologies for measuring human blood pressure are limited when used in situations where extreme vibration or acoustic noise is present. In this study, human subjects were used to establish the effectiveness of a novel adaptive blood pressure monitoring (ABPM) syste...
journal_title：Annals of biomedical engineering
pub_type： 临床试验,杂志文章
doi：10.1114/1.1481051
更新日期：2002-05-01 00:00:00
abstract：:Mitral valve regurgitation (MVR) is common in patients receiving left ventricular assist device (LVAD) support, however the haemodynamic effect of MVR is not entirely clear. This study evaluated the haemodynamic effect of MVR with LVAD support and the influence of inflow cannulation site and LVAD speed modulation. Lef...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1579-5
更新日期：2016-09-01 00:00:00
abstract：:Magnetic (eddy-current) stimulation of the inspiratory motor nerves in the neck of the anesthetized dog was achieved. Using a 10-turn coil wound around the base of the neck and a train of pulses (25/s), inspiration was produced by tetanic contraction of the inspiratory muscles. The volume of air inspired increased wit...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368176
更新日期：1993-05-01 00:00:00
abstract：:Uncomplicated acute type B aortic dissections are usually treated medically, but they can become acutely complicated by rapid expansion, rupture and malperfusion syndromes and in the longer term by chronic dilatation and aortic aneurysm formation. The objective of this study is to use computational fluid dynamics reco...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-014-1075-8
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND:We present a fundamental theoretical framework for analysis of energy dissipation in any component of the circulatory system and formulate the full energy budget for both venous and arterial circulations. New indices allowing disease-specific subject-to-subject comparisons and disease-to-disease hemodynamic ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-009-9650-0
更新日期：2009-04-01 00:00:00
abstract：:The false vocal folds are believed to be components of the acoustic filter that is responsible for shaping the voice. However, the effects of false vocal folds on the vocal fold vibration and the glottal aerodynamic during phonation remain unclear. This effect has implications for computational modeling of phonation a...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-008-9630-9
更新日期：2009-03-01 00:00:00
