Abstract：
:Diluted serum provides a model of interstitial fluid that can be used to study the response of smooth muscle cells (SMCs) to interstitial flow. The effect of serum and some of its components on SMC contraction (area reduction) and intracellular calcium ([Ca2+]i) response were characterized in rat aortic SMC in vitro. Rat aortic SMCs contracted dramatically to fetal bovine serum (FBS), bovine serum albumin (BSA), and lysophosphatidic acid (LPA) within 5 min of exposure. By 30 min, cell areas were significantly reduced. Even at concentrations as low as 0.0005% FBS, 0.004% BSA and 0.25 microM LPA, cell areas were significantly different from controls at 30 min. The [Ca2+]i response was significant for serum and LPA at these low concentration levels, but BSA did not elicit a significant [Ca2+]i response at concentrations of 0.1% or lower. Under calcium controlled conditions in which SMCs were pretreated with 10 microM BAPTA-AM, contraction levels were not statistically different from non-calcium controlled conditions even when SMCs were exposed to the highest concentration of serum, BSA, or LPA. It appears that LPA and albumin are components of interstitial fluid that contribute to SMC contraction through calcium-independent mechanisms.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Ainslie K,Shi ZD,Garanich JS,Tarbell JMdoi
10.1007/s10439-004-7820-7subject
Has Abstractpub_date
2004-12-01 00:00:00pages
1667-75issue
12eissn
0090-6964issn
1573-9686journal_volume
32pub_type
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