Abstract：
:Recent evidence suggests that energy substrate metabolism forms the link between gene expression and contractile function of the heart. This hypothesis draws on three seemingly unconnected observations, made in 1941 by three different investigators. The first gave rise to the concept of the dynamic nature of body constituents, the second to the concept of one gene for one enzyme, and the third to the concept of energy conservation in the phosphate bond of adenosine triphosphate (ATP), the "approximate P." The heart employs different mechanisms to adapt to acute and to sustained changes in energy demand. While acute changes in energy demand are met by the coordinated activation or inactivation of enzymes, chronic changes in energy demand are met by adjustments in the rate of "metabolic" gene expression which are often isoform specific. Transcriptional analysis by quantitative polymerase chain reaction (PCR) defines changes in energy substrate metabolism in response to an altered physiologic environment at the transcriptional level. The paradigm of cardiac hypertrophy and atrophy has revealed a surprisingly uniform and rapid response in cardiac gene expression including the reactivation of fetal metabolic genes. The physiologic consequences are revealed in a shift in substrate utilization from fatty acids to glucose. Clinical consequences include a change in metabolic gene expression of the failing human heart, including the downregulation of glucose transporters and the enzymes of fatty acid metabolism. Although it is possible to study regulation of metabolism at the level of gene expression, the complexities of the interactions between metabolic intermediates and transcription factors may be difficult to elucidate by simple reductionist approaches. A model of feedback/feedforward between genes and proteins is needed to explain the principle of reestablishing homeostatic control of metabolism as cellular and external environments change.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Taegtmeyer Hdoi
10.1114/1.1312187subject
Has Abstractpub_date
2000-08-01 00:00:00pages
871-6issue
8eissn
0090-6964issn
1573-9686journal_volume
28pub_type
杂志文章,评审abstract：:Left-right (LR) asymmetry is a biologically conserved property in living organisms that can be observed in the asymmetrical arrangement of organs and tissues and in tissue morphogenesis, such as the directional looping of the gastrointestinal tract and heart. The expression of LR asymmetry in embryonic tissues can be ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-015-1431-3
更新日期：2016-05-01 00:00:00
abstract：:Elastin is an essential component of arteries which provides structural integrity and instructs smooth muscle cells to adopt a quiescent state. Despite interaction of endothelial cells with elastin in the internal elastic lamina, the potential for exploiting this interaction therapeutically has not been explored in de...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0142-z
更新日期：2011-01-01 00:00:00
abstract：:Current induced in cultured cerebellar granule cells by the bath application of kainate (500 microM) was measured using the conventional patch-clamp technique. Two different kinds of responses were observed after the agonist perfusion. Some cells exhibited small inward whole-cell currents: 116 +/- 40 pA (7 cells) at a...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02684186
更新日期：1997-05-01 00:00:00
abstract：:Numerous head injury models exist that vary in mesh density by orders of magnitude. A careful study of the mesh convergence behavior is necessary, especially in terms of strain most relevant to brain injury. To this end, as well as to investigate the effect of element integration scheme on simulated strains, we re-mes...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-018-02159-z
更新日期：2019-02-01 00:00:00
abstract：:Microstructural characterization of cardiac tissue and its remodeling in disease is a crucial step in many basic research projects. We present a comprehensive approach for three-dimensional characterization of cardiac tissue at the submicrometer scale. We developed a compression-free mounting method as well as labelin...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-015-1465-6
更新日期：2016-05-01 00:00:00
abstract：:Although diagnostic testing with auditory evoked potentials (EPs) has become routine, quantitative measurements of signal and noise are still lacking. In this study, current signal, power, noise power, and signal-to-noise ratio (SNR) estimation formulas are reviewed and applied to auditory brainstem response averaging...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02684183
更新日期：1996-11-01 00:00:00
abstract：:Previous research in cellular adhesion has focused primarily on studying isolated cells under conditions where cells do not interact with each other. However, in vivo cells form sheets where both cell-substratum and cell-cell interactions contribute to the overall adhesive behavior. Our understanding of how cell-cell ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.178
更新日期：1999-03-01 00:00:00
abstract：:Measurement of cell shortening is an important technique for assessment of physiology and pathophysiology of cardiac myocytes. Many types of heart disease are associated with decreased myocyte shortening, which is commonly caused by structural and functional remodeling. Here, we present a new approach for local measur...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1593-7
更新日期：2016-10-01 00:00:00
abstract：:Impedance cardiography has not achieved popularity in the Intensive Care Unit (ICU) to date probably because of the limitations in technique and interpretation associated with the altered physiology of critically ill patients, and also because of interference from other machinery in the ICU. The current climate of que...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368068
更新日期：1989-01-01 00:00:00
abstract：:A two-dimensional sheet model was used to study the dynamics of reentry around a zone of functional block. The sheet is a set of parallel, continuous, and uniform cables, transversely interconnected by a brick-wall arrangement of fixed resistors. In accord with experimental observations on cardiac tissue, longitudinal...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368286
更新日期：1994-11-01 00:00:00
abstract：:High mechanical stress in atherosclerotic plaques at vulnerable sites, called critical stress, contributes to plaque rupture. The site of minimum fibrous cap (FC) thickness (FC(MIN)) and plaque shoulder are well-documented vulnerable sites. The inherent weakness of the FC material at the thinnest point increases the s...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0078-3
更新日期：2010-10-01 00:00:00
abstract：:In this paper, we present the application of a semi-global inverse method for determining material parameters of biological tissues. The approach is based on the successive response surface method, and is illustrated by fitting constitutive parameters to two nonlinear anisotropic constitutive equations, one for aortic...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-005-8338-3
更新日期：2005-12-01 00:00:00
abstract：:Intraluminal thrombus (ILT) is a pseudo-tissue that develops from coagulated blood, and is found in most abdominal aortic aneurysms (AAAs) of clinically relevant size. A number of studies have suggested that ILT mechanical characteristics may be related to AAA risk of rupture, even though there is still great controve...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-015-1267-x
更新日期：2015-09-01 00:00:00
abstract：:Neuromuscular impairment associated with cerebral palsy (CP) often leads to life-long walking deficits. Our goal was to evaluate the ability of a novel untethered wearable ankle exoskeleton to reduce the severity of gait pathology from CP. In this clinical feasibility study of five individuals with CP, we used instrum...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-019-02237-w
更新日期：2019-06-01 00:00:00
abstract：:Polycrystalline silicon (poly-Si) micromechanical structures can be made by selectively etching an underlying sacrificial oxide layer. Advantages of this micromachining technique are its simplicity and compatibility with conventional integrated-circuit processing. Compressive internal stress in poly-Si films constrain...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02584269
更新日期：1986-01-01 00:00:00
abstract：:Quantifying injury tolerance for concussion is complicated by variability in the type, severity, and time course of post-injury physiological and behavioral changes. The current study outlined acute and chronic changes in behavioral metrics following rotational acceleration-induced concussion in rats. The Medical Coll...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1647-x
更新日期：2016-11-01 00:00:00
abstract：:Computing the numerical solution of the bidomain equations is widely accepted to be a significant computational challenge. In this study we extend a previously published semi-implicit numerical scheme with good stability properties that has been used to solve the bidomain equations (Whiteley, J.P. IEEE Trans. Biomed. ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-008-9513-0
更新日期：2008-08-01 00:00:00
abstract：:We have developed a method for quantifying the complexity of activation patterns observed during ventricular fibrillation (VF) that is based on our previously reported methodology for decomposing epicardial mapping data into a set of isolated wavefronts. One-half second datasets are acquired from a 21 x 24 array of un...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02684160
更新日期：1997-09-01 00:00:00
abstract：:A multifunctional FePt nanoparticle was developed that targets tumor microvasculature via "radiation-guided" peptides, and is detected by both near-infrared (NIR) fluorescence imaging and analytical mass spectrometry methods. Tumor specific binding was first measured by biotinylated peptide linked to fluorophore-conju...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0219-8
更新日期：2011-03-01 00:00:00
abstract：:Based on mathematical expressions governing the electric field, the extracellular potentials generated by a single active fiber in a restricted circular cylindrical volume conductor are evaluated. This paper examines the effect of the extent of the volume conductor, with radius b, on the extracellular potentials at di...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368439
更新日期：1990-01-01 00:00:00
abstract：:Spine musculoskeletal models used to estimate loads and displacements require many simplifying assumptions. We examined how assumptions about subject size and vertebral positions can affect the model outcomes. Head and neck models were developed to represent 30 subjects (15 males and 15 females) in neutral posture and...
journal_title：Annals of biomedical engineering
pub_type： 临床试验,杂志文章
doi：10.1007/s10439-018-2084-9
更新日期：2018-11-01 00:00:00
abstract：:Ever-increasing demand for bone grafts necessitates the realization of clinical implementation of bone tissue engineered constructs. The predominant hurdle to implementation remains to be securing FDA approval, based on the lack of viable methods for the rigorous monitoring of said constructs. The study presented here...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-017-1937-y
更新日期：2018-01-01 00:00:00
abstract：:In vitro stent-induced thromboembolism was altered by the presence of residual stenoses placed upstream or placed upstream and downstream of the stent. Heparinized (3 micro/ml) bovine blood was gravity fed through a conduit with a deployed coronary stent. Embolism was continuously monitored using a light-scattering mi...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.243
更新日期：2000-02-01 00:00:00
abstract：:High density particle image velocimetry, with spatial resolution of O(1 mm), was used to measure the effect of occluder rebound on the flow field near a Bjork-Shiley Monostrut tilting-disk mitral valve. The ability to measure two velocity components over an entire plane simultaneously provides a very different insight...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.281
更新日期：2000-04-01 00:00:00
abstract：:There has been considerable debate on the relative importance of biochemical stimuli and mechanical deformation in neutrophil adhesion in lung capillaries, a process observed following bacterial infection in the body. In contrast to venules, where the vessel diameter is larger than the leukocyte diameter (6-9 microm) ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-008-9437-8
更新日期：2008-04-01 00:00:00
abstract：:The bending stiffness of a phospholipid bilayer (Kc) was measured by forming thin bilayer cylinders (tethers) from giant phospholipid vesicles. Based on the balance of forces, the tether force was expected to be proportional to the square root of the membrane tension, with a constant of proportionality containing Kc. ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02684228
更新日期：1996-09-01 00:00:00
abstract：:A flow diverter (FD) is a flexible, densely braided stent-mesh device placed endoluminally across an intracranial aneurysm to induce its thrombotic occlusion. FD treatment planning using computational virtual stenting and flow simulation requires accurate representation of the expanded FD geometry. We have recently de...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-013-0808-4
更新日期：2013-10-01 00:00:00
abstract：:Dual X-ray absorptiometry (DXA) measures areal bone mineral density (aBMD) by simplifying a complex 3D bone structure to a 2D projection and is not equally effective for explaining fracture strength in women and men. Unlike DXA, subject-specific quantitative computed tomography-based finite element analysis (QCT/FEA) ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-017-1914-5
更新日期：2017-12-01 00:00:00
abstract：:This study presents a new steady-state visual evoked potential (SSVEP)-based brain computer interface (BCI). SSVEPs, induced by phase-tagged flashes in eight light emitting diodes (LEDs), were used to control four cursor movements (up, right, down, and left) and four button functions (on, off, right-, and left-clicks)...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-9964-y
更新日期：2010-07-01 00:00:00
abstract：:Biodegradable implants have demonstrated clinical success in simple applications (e.g., absorbable sutures) and have shown great potential in many other areas of interventional medicine, such as localized drug delivery, engineered tissue scaffolding, and structural implants. For endovascular stenting and musculoskelet...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-015-1477-2
更新日期：2016-02-01 00:00:00
