Semliki Forest virus produced in the absence of the 6K protein has an altered spike structure as revealed by decreased membrane fusion capacity.

abstract：:The promotion of membrane fusion by the paramyxovirus hemagglutinin-neuraminidase (HN) and fusion (F) proteins requires that they be derived from homologous viruses, suggesting the possibility that the promotion of fusion requires a virus-specific communication between the two glycoprotein spikes. We have evaluated th...

journal_title：Virology

authors： Deng R,Wang Z,Mirza AM,Iorio RM

更新日期：1995-06-01 00:00:00

abstract：:Stress granule (SG)-like antiviral granules (AVG) had been found in some vaccinia virus infection conditions and shown to repress translation. Similar RNA granules are also associated with translational inhibition and poxvirus restriction mediated by the host restriction factor SAMD9, but their function is less clear....

journal_title：Virology

authors： Meng X,Xiang Y

更新日期：2019-03-01 00:00:00

abstract：:The complete sequence of the genome of the cherry strain of tomato bushy stunt virus (TBSV), a member of the tombusvirus group, was determined. A full-length clone of the genome containing a bacteriophage T7 RNA polymerase promoter was assembled from partial cDNA clones. In vitro transcripts of the genome, either with...

journal_title：Virology

authors： Hearne PQ,Knorr DA,Hillman BI,Morris TJ

更新日期：1990-07-01 00:00:00

abstract：:Recent studies have shown that interferon-gamma (IFN-gamma) synergizes with IFN-alpha/beta to inhibit the replication of both RNA and DNA viruses. We investigated the effects of IFNs on the replication of two strains of severe acute respiratory syndrome-associated coronavirus (SARS-CoV). While treatment of Vero E6 cel...

journal_title：Virology

authors： Sainz B Jr,Mossel EC,Peters CJ,Garry RF

更新日期：2004-11-10 00:00:00

abstract：:Brain metastases occur commonly in the setting of a variety of human cancers. At present, such cases are invariably fatal and highlight a need for research on new therapies. We have developed a mouse brain tumor model utilizing the Harding-Passey melanoma cell line injected intracranially into C57Bl/6 mice. Tumors dev...

journal_title：Virology

authors： Randazzo BP,Kesari S,Gesser RM,Alsop D,Ford JC,Brown SM,Maclean A,Fraser NW

更新日期：1995-08-01 00:00:00

abstract：:Herpes simplex virus mutant KG111 contains a nonsense mutation at codon 44 of the viral thymidine kinase (tk) gene and produces low amounts of a truncated tk polypeptide. We tested mutant KG111 and related viruses that specify varying amounts of similar truncated tk polypeptides for their sensitivities to antiviral nu...

journal_title：Virology

authors： Coen DM,Irmiere AF,Jacobson JG,Kerns KM

更新日期：1989-02-01 00:00:00

abstract：:To investigate differential evolutionary rates and selective forces of WNV in hosts and vectors, we measured the genetic diversity that arose during alternating passage in mosquitoes and birds. Within-host genetic diversity was monitored in each of three experimentally passed replicates, and the complete genome sequen...

journal_title：Virology

authors： Jerzak GV,Brown I,Shi PY,Kramer LD,Ebel GD

更新日期：2008-05-10 00:00:00

abstract：:An essential step for a productive infection by the dengue flavivirus (DEN) is translation of the m(7)G-capped, nonpolyadenylated positive-sense RNA genome. We have recently identified sequences within the DEN 3' untranslated region (UTR) that modulate viral translation. Here, we show that the DEN type 2 (DEN2) 3'UTR ...

journal_title：Virology

authors： Holden KL,Harris E

更新日期：2004-11-10 00:00:00

abstract：:Lentiviral vectors have gained much attention in recent years mainly because they integrate into nondividing host-cell genomes. For clinical applications, a safe and efficient lentiviral vector system is required. Previously, we have established a human immunodeficiency virus type 1 (HIV-1)-derived three-plasmid lenti...

journal_title：Virology

authors： Iwakuma T,Cui Y,Chang LJ

更新日期：1999-08-15 00:00:00

abstract：:The Epstein-Barr virus (EBV) BGLF4 gene product is the only protein kinase encoded by the virus genome. In order to elucidate its physiological roles in viral productive replication, we here established a BGLF4-knockout mutant and a revertant virus. While the levels of viral DNA replication of the deficient mutant wer...

journal_title：Virology

authors： Murata T,Isomura H,Yamashita Y,Toyama S,Sato Y,Nakayama S,Kudoh A,Iwahori S,Kanda T,Tsurumi T

更新日期：2009-06-20 00:00:00

abstract：:MuSV349 is a TB-cell line which produces infectious MuSV with little or no MuLV detectable by the XC assay. The apparent restriction of MuLV replication in MuSV349 cells was investigated. A replication-competent helper virus, with protein composition nearly identical to that of Mo-MuLV was isolated from the viruses pr...

journal_title：Virology

authors： Yuen PH,Soong MM,Kissil MS,Wong PK

更新日期：1984-01-30 00:00:00

abstract：:Homologous, subgenomic fragments of the viral LCR and E6/E7 transforming genes of HPV-18 and HPV-16 were amplified from several primary cervical, penile, and vulvar tumors and cloned into a pUC-18-derived vector. When assayed by a quantitative transformation assay using primary human keratinocytes, the subgenomic regi...

journal_title：Virology

authors： Villa LL,Schlegel R

更新日期：1991-03-01 00:00:00

abstract：:HIV-1 Nef is expressed in astrocytes, but a contribution to neuropathogenesis and the development of HIV-associated dementia (HAD) remains uncertain. To determine the neuropathogenic actions of the HIV-1 Nef protein, the brain-derived (YU-2) and blood-derived (NL4-3) Nef proteins were expressed in neural cells using a...

journal_title：Virology

authors： van Marle G,Henry S,Todoruk T,Sullivan A,Silva C,Rourke SB,Holden J,McArthur JC,Gill MJ,Power C

更新日期：2004-11-24 00:00:00

abstract：:Electron microscopy and sequence analysis have suggested the presence of distinct morphological regions within the reovirus cell attachment protein sigma 1. Kinking of purified sigma 1 observed by electron microscopy and sequence-predicted flexibility profiles suggest the presence of potential flexible regions in the ...

journal_title：Virology

authors： Duncan R,Lee PW

更新日期：1994-08-15 00:00:00

abstract：:The human hepatitis B Virus genome (HBV) contains a liver-specific enhancer upstream of the X ORF which has been studied in detail by several investigators. A second liver-specific enhancer element, designated here as enhancer II, has been relatively recently described in the HBV genome, which is located within the co...

journal_title：Virology

authors： López-Cabrera M,Letovsky J,Hu KQ,Siddiqui A

更新日期：1991-08-01 00:00:00

abstract：:cDNA clones encoding the envelope glycoprotein E1 of rubella virus (RV) were altered by site-directed mutagenesis at consensus sites for addition of N-linked glycans. The resulting plasmids were introduced into COS cells and the mutant E1 proteins were analyzed by indirect immunofluorescence, radioimmunoprecipitation,...

journal_title：Virology

authors： Hobman TC,Qiu ZY,Chaye H,Gillam S

更新日期：1991-04-01 00:00:00

abstract：:The baculovirus-insect cell system is used routinely for foreign glycoprotein production, but the precise nature of the N-glycosylation pathway in this system remains unclear. Some studies indicate that these cells cannot process N-linked oligosaccharides to complex forms containing outer-chain galactose and sialic ac...

journal_title：Virology

authors： Jarvis DL,Finn EE

更新日期：1995-10-01 00:00:00

abstract：:West Nile virus (WNV) is a mosquito-borne flavivirus that causes febrile illness, encephalitis, and occasionally death in humans. The envelope protein is the main component of the WNV virion surface, and domain III of the envelope protein (EIII) is both a putative receptor binding domain and a target of highly specifi...

journal_title：Virology

authors： Plante JA,Torres M,Huang CY,Beasley DWC

更新日期：2016-09-01 00:00:00

abstract：:Previous in vitro work demonstrated the incorporation of the major inducible 70k heat shock protein (i.e., 72k HSP) into the biologically active light nucleocapsid (L-NC) variant of canine distemper virus (CDV). Here, in vitro induction of the cellular stress response, characterized by elevated cytoplasmic and intranu...

journal_title：Virology

authors： Oglesbee MJ,Kenney H,Kenney T,Krakowka S

更新日期：1993-02-01 00:00:00

abstract：:Infection of different human epithelial cell lines with human respiratory syncytial virus (HRSV) revealed significant differences in the electrophoretic mobility of the viral attachment glycoprotein (G). Cell-type specific differences in G protein glycosylation were observed with certain lectins and sugar-specific rea...

journal_title：Virology

authors： García-Beato R,Martínez I,Francí C,Real FX,García-Barreno B,Melero JA

更新日期：1996-07-15 00:00:00

abstract：:The parapoxvirus Orf virus (ORFV) encodes several immunomodulatory proteins (IMPs) that modulate host-innate and pro-inflammatory responses and has been proposed as a vaccine delivery vector for use in animal species. Here we describe the construction and characterization of two recombinant ORFV vectors expressing the...

journal_title：Virology

authors： Martins M,Joshi LR,Rodrigues FS,Anziliero D,Frandoloso R,Kutish GF,Rock DL,Weiblen R,Flores EF,Diel DG

更新日期：2017-11-01 00:00:00

abstract：:Measles virus V protein represses genome replication through a poorly understood mechanism, which led us to investigate whether V protein might be an RNA-binding modulatory factor. Recombinant V protein, expressed from transfected HEp-2 cells or E. coli, formed protein-RNA complexes with poly-guanosine (poly-G) or pol...

journal_title：Virology

authors： Parks CL,Witko SE,Kotash C,Lin SL,Sidhu MS,Udem SA

更新日期：2006-04-25 00:00:00

abstract：:We reported previously that carbohydrate attachment to an overlapping glycosylation site adjacent to the signal peptide of GP3 from equine arteritis virus (EAV) prevents cleavage. Here we investigated whether this unusual processing scheme is a feature of GP3s of other Arteriviridae, which all contain a glycosylation ...

journal_title：Virology

authors： Zhang M,Veit M

更新日期：2018-04-01 00:00:00

abstract：:Human enteric adenovirus Ad41 is associated with children gastroenteritis. To infect gastrointestinal cells, the invading virus must be acid-stable and resistant to inactivation by bile salts and proteases. In addition, it has to cross the mucus barrier before it infects mucosa cells. We show that Ad41 infectivity is ...

journal_title：Virology

authors： Favier AL,Burmeister WP,Chroboczek J

更新日期：2004-04-25 00:00:00

abstract：:We have investigated the interaction of SV40 virions with polarized monkey kidney epithelial cells. Virions were tagged with biotin to facilitate their detection and were found to retain full infectivity. When polarized Vero C1008 cells were incubated with biotinylated virions followed by a strepavidin-rhodamine conju...

journal_title：Virology

authors： Basak S,Turner H,Compans RW

更新日期：1992-09-01 00:00:00

abstract：:Viral metagenomics coupled to high-throughput sequencing has provided a powerful tool for large-scale detection of known and unknown viruses associated to distinct hosts and environments. Using this approach, known and novel viruses have been characterized from sylvatic and commercial avian hosts, increasing our under...

journal_title：Virology

authors： Cibulski S,Weber MN,de Sales Lima FE,Lima DA,Fernandes Dos Santos H,Teixeira TF,Varela APM,Tochetto C,Mayer FQ,Roehe PM

更新日期：2020-09-01 00:00:00

abstract：:Ovine pulmonary carcinoma is a contagious neoplastic disease of sheep which can be transmitted experimentally with cell-free filtrate. Products homologous to structural proteins of type D and type B retroviruses are expressed in the tumors, but an infectious retrovirus has yet to be isolated. Retroviral RNA was isolat...

journal_title：Virology

authors： Hecht SJ,Carlson JO,DeMartin JC

更新日期：1994-07-01 00:00:00

abstract：:Equine infectious anemia virus (EIAV) is responsible of acute disease episodes characterized by fever, anemia, thrombocytopenia and anorexia in equids. The high mutation rate in EIAV genome limited the number of full genome sequences availability. In the present study, we used the SureSelect target enrichment system w...

journal_title：Virology

authors： Deshiere A,Berthet N,Lecouturier F,Gaudaire D,Hans A

更新日期：2019-11-01 00:00:00

abstract：:Missing contact footprinting with formic acid as a modifying reagent was used to examine specific IE-1 binding contacts to double-stranded oligonucleotides that contained either a consensus hr repeat sequence or a sequence from the pe38 promoter, which is down regulated by IE-1. The hr repeat sequences contain two con...

journal_title：Virology

authors： Leisy DJ,Rohrmann GF

更新日期：2000-08-15 00:00:00

abstract：:The target for neutralizing antibodies against human immunodeficiency virus (HIV) is the trimeric Env protein on the native virion. Conserved neutralizing epitopes of receptor binding sites are located in the recessed core of the Env protein, partially masked by glycosylations and variable loops. In this study, we hav...

journal_title：Virology

authors： Kang SM,Quan FS,Huang C,Guo L,Ye L,Yang C,Compans RW

更新日期：2005-01-05 00:00:00