Attenuation of lpr-graft-versus-host disease (GVHD) in MRL/lpr spleen cell-injected SCID mice by in vivo treatment with anti-V beta 8.1,2 monoclonal antibody.


:When MRL/lpr (H-2k) spleen cells were intraperitoneally injected into C.B-17-scid/scid (severe combined immunodeficient (SCID)) (H-2d) mice, the SCID (SCID-MRL/lpr) mice manifested a severe wasting syndrome with weight loss, splenic atrophy, and lymphoid cell infiltration in the liver and lung, as seen in lpr-GVHD. In contrast, MRL/+ spleen cell-injected SCID (SCID-MRL/+) mice did not show lpr-GVHD. The spleens of SCID-MRL/lpr mice showed progressive increases in donor CD4+ and CD8+ T cells from 4 to 12 weeks after injection and a decrease in B cells at 12 weeks. SCID-MRL/+ mice showed a stable engraftment of CD4+ and CD8+ T cells and a progressive increase in B cells. Analyses of T cell receptor (TCR) repertoires (V beta 6, V beta 8.1,2 and V beta 11) revealed that the V beta 8.1,2+ T cells were found more frequently in SCID-MRL/lpr mice than in SCID-MRL/+ mice. When SCID-MRL/lpr mice were treated with intraperitoneal injection of an anti-V beta 8.1,2 (KJ16) MoAb, V beta 8.1,2+ T cells were markedly depleted, and the severity of lpr-GVHD was attenuated at 4 and 8 weeks after treatment, in contrast to normal rat IgG-injected SCID-MRL/lpr mice. However, the KJ16 MoAb-treated SCID-MRL/lpr mice suffered from severe lpr-GVHD 12 weeks after treatment, although V beta 8.1,2+ T cells were still maintained at a low level. These findings suggest that V beta 8.1,2+ T cells are a major T cell population that mediates lpr-GVHD in the early stage of lpr-GVHD, but that in the later stage, the other T cell populations may proliferate naturally or in accordance with the depletion of V beta 8.1,2+ T cells, and contribute to the development of lpr-GVHD.


Clin Exp Immunol


Hosaka N,Nagata N,Miyashima S,Ikehara S




Has Abstract


1994-06-01 00:00:00












  • Recombinant proteinase 3 (Wegener's antigen) expressed in Pichia pastoris is functionally active and is recognized by patient sera.

    abstract::The open reading frame of human proteinase 3 (PR3) without the prepro-peptide was cloned and expressed in Escherichia coli (rcPR3) and in Pichia pastoris (rpPR3). The 6-histidine tagged rpPR3 was efficiently secreted into culture supernatant from which it could be purified by immobilized metal chelate chromatography. ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Harmsen MC,Heeringa P,van der Geld YM,Huitema MG,Klimp A,Tiran A,Kallenberg CG

    更新日期:1997-11-01 00:00:00

  • Cellular immunological changes in patients with LADA are a mixture of those seen in patients with type 1 and type 2 diabetes.

    abstract::There is currently scarce knowledge of the immunological profile of patients with latent autoimmune diabetes mellitus in the adult (LADA) when compared with healthy controls (HC) and patients with classical type 1 diabetes (T1D) and type 2 diabetes (T2D). The objective of this study was to investigate the cellular imm...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Singh K,Martinell M,Luo Z,Espes D,Stålhammar J,Sandler S,Carlsson PO

    更新日期:2019-07-01 00:00:00

  • The effect of adrenomedullin and cold stress on interleukin-6 levels in some rat tissues.

    abstract::Stress known to stimulate sympathetic activity, as well as the hypothalamic-pituitary-adrenal axis (HPA), produces a significant increase in adrenomedullin (AdM) levels, suggesting a regulatory or protective role for AdM in countering HPA activation that follows a variety of stressors. Stressors can modulate the secre...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Yildirim NC,Yurekli M

    更新日期:2010-07-01 00:00:00

  • Anti-lymphocyte antibodies in lethal mouse malaria. II. Induction of an autoantibody specific suppressor T cell by non-lethal P. yoelii.

    abstract::The anti-lymphocyte autoantibody response to irradiated lethal Plasmodium berghei malaria parasites in normal mice was significantly reduced when recipients were pre-treated with splenic T cells from mice recovered from a non-lethal Plasmodium yoelii infection. Suppression was specific for the autoantibody and did not...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: de Souza JB,Playfair JH

    更新日期:1983-10-01 00:00:00

  • Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: position statement of the T-Cell Workshop Committee of the Immunology of Diabetes Society.

    abstract::Autoimmune T cell responses directed against insulin-producing β cells are central to the pathogenesis of type 1 diabetes (T1D). Detection of such responses is therefore critical to provide novel biomarkers for T1D 'immune staging' and to understand the mechanisms underlying the disease. While different T cell assays ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Mallone R,Mannering SI,Brooks-Worrell BM,Durinovic-Belló I,Cilio CM,Wong FS,Schloot NC,T-Cell Workshop Committee, Immunology of Diabetes Society.

    更新日期:2011-01-01 00:00:00

  • Up-regulation of the tumour-associated marker CD44V6 in experimental kidney disease.

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    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Takazoe K,Foti R,Tesch GH,Hurst LA,Lan HY,Atkins RC,Nikolic-Paterson DJ

    更新日期:2000-09-01 00:00:00

  • Impairment of circulating myeloid dendritic cells in immunosuppressed liver transplant recipients.

    abstract::The aim of the present study was to elucidate the impact of liver transplantation (LTX) on myeloid dendritic cell (MDC) homeostasis. We observed a threefold reduction of circulating CD1c(+) MDC immediately after LTX (n = 16; P < 0.01), and normalization between 3 and 12 months after LTX. This decline was not due to re...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Bosma BM,Metselaar HJ,Tra WM,Mancham S,Kuipers EJ,Tilanus HW,Kwekkeboom J

    更新日期:2007-09-01 00:00:00

  • Low mannose-binding lectin (MBL) levels in neonates with pneumonia and sepsis.

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    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Frakking FN,Brouwer N,van Eijkelenburg NK,Merkus MP,Kuijpers TW,Offringa M,Dolman KM

    更新日期:2007-11-01 00:00:00

  • What does the future hold for clinical studies in vasculitis?

    abstract::The era prior to 1990 was a time of careful observation of disease presentation, course, outcomes and meticulous pathology studies. These mainly single-centre studies introduced new life-saving therapies for drugs still used effectively today. In the 1970-1980s, cyclophosphamide (CyP) added to glucocorticosteroids (GC...

    journal_title:Clinical and experimental immunology

    pub_type: 历史文章,杂志文章,评审


    authors: Hoffman GS

    更新日期:2011-05-01 00:00:00

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    abstract::GPI-linked surface molecules have recently been described as structures with an activation potential for human T lymphocytes. To study the role of these molecules in T cell activation we analysed GPI-deficient or normal T cells from patients with paroxysmal nocturnal haemoglobinuria (PNH). On activation with allogenei...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Schubert J,Stroehmann A,Scholz C,Schmidt RE

    更新日期:1995-10-01 00:00:00

  • Negative regulation of inflammatory responses by immunoglobulin A receptor (FcαRI) inhibits the development of Toll-like receptor-9 signalling-accelerated glomerulonephritis.

    abstract::Myeloid FcαRI, a receptor for immunoglobulin (Ig)A, mediates cell activation or inhibition depending on the type of ligand interaction, which can be either multivalent or monovalent. Anti-inflammatory signalling is triggered by monomeric targeting using anti-FcαRI Fab or IgA ligand binding, which inhibits immune and n...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Watanabe T,Kanamaru Y,Liu C,Suzuki Y,Tada N,Okumura K,Horikoshi S,Tomino Y

    更新日期:2011-11-01 00:00:00

  • Phagocytic efficacy of macrophage-like cells as a function of cell cycle and Fcgamma receptors (FcgammaR) and complement receptor (CR)3 expression.

    abstract::Previous studies have shown that the efficiency of phagocytosis is a function of cell cycle and that phagocytosis promotes cell cycle progression. Because phagocytosis is dependent on cellular receptors we hypothesized that Fcgamma receptors (FcgammaR) and complement receptors (CR) expression varied with cell cycle. C...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Luo Y,Cook E,Fries BC,Casadevall A

    更新日期:2006-08-01 00:00:00

  • Somatic mutation of immunoglobulin V(H)6 genes in human infants.

    abstract::Infants respond to antigen by making antibody that is generally of low affinity for antigen. Somatic hypermutation of immunoglobulin genes, and selection of cells expressing mutations with improved affinity for antigen, are the molecular and cellular processes underlying the maturation of antibody affinity. We have re...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Ridings J,Dinan L,Williams R,Roberton D,Zola H

    更新日期:1998-10-01 00:00:00

  • Delayed disease progression in HIV-2: the importance of TRIM5α and the retroviral capsid.

    abstract::HIV-2 is thought to have entered the human population in the 1930s through cross-species transmission of SIV from sooty mangabeys in West Africa. Unlike HIV-1, HIV-2 has not led to a global pandemic, and recent data suggest that HIV-2 prevalence is declining in some West African states where it was formerly endemic. A...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Boswell MT,Rowland-Jones SL

    更新日期:2019-06-01 00:00:00

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    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Ferrer JM,Iglesias J,Hernández M,Matamoros N

    更新日期:1995-11-01 00:00:00

  • Cytokine production by peripheral blood mononuclear cells in IgA nephropathy.

    abstract::The regulation of cytokine production and T cell proliferation by other cytokines is mandatory in mediating inflammatory responses but the full understanding is far from complete. We have previously reported increased production of IL-2 and IL-2 receptors (IL-2R) in IgA nephropathy. The present study was undertaken to...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Lai KN,Leung JC,Li PK,Lui SF

    更新日期:1991-08-01 00:00:00

  • Percentile ranges for serum IgG subclass concentrations in healthy Chinese children.

    abstract::In order to establish normal reference ranges of serum immunoglobulin G subclasses for different age groups in Chinese, we measured the four IgG subclasses by radial immunodiffusion using polyclonal antisera in 350 normal healthy subjects (148 males and 202 females) recruited from the community. There was no significa...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Lau YL,Jones BM,Ng KW,Yeung CY

    更新日期:1993-02-01 00:00:00

  • Cell-bound helper and suppressor factors in primate lymphocytes.

    abstract::Cell-bound helper and suppressor factors, on lymphocytes from Rhesus monkeys, were assayed by the indirect immunofluorescent method, using F(ab')2 fragments of rabbit antisera to antigen specific secreted helper and suppressor factors. The rabbit antisera recognize the function related to the 'constant' region of secr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Lehner T,Lamb JR,Kontiainen S

    更新日期:1982-03-01 00:00:00

  • Hepatitis B surface antigen containing immune complexes occur in seronegative hepatocellular carcinoma patients.

    abstract::IgG, IgM and hepatitis B surface antigen (HBsAg) containing immune complexes (IC) were detected by the Clq and conglutinin solid phase assays in both HBsAg+ and HBsAg- groups of patients with primary hepatocellular carcinoma (HCC). No differences were observed between the two patient groups either in the levels of ant...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Brown SE,Howard CR,Steward MW,Ajdukiewicz AB,Whittle HC

    更新日期:1984-02-01 00:00:00

  • The role of the immune system in kidney disease.

    abstract::The immune system and the kidneys are closely linked. In health the kidneys contribute to immune homeostasis, while components of the immune system mediate many acute forms of renal disease and play a central role in progression of chronic kidney disease. A dysregulated immune system can have either direct or indirect...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Tecklenborg J,Clayton D,Siebert S,Coley SM

    更新日期:2018-05-01 00:00:00

  • Immunomodulatory effects of Lactobacillus plantarum colonizing the intestine of gnotobiotic rats.

    abstract::We have studied the effect of the probiotic strain Lactobacillus plantarum 299v on the immune functions of gnotobiotic rats. One group of germ-free rats was colonized with the type 1-fimbriated Escherichia coli O6:K13:H1 and another group with the same E. coli strain together with L. plantarum 299v. One and 5 weeks af...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Herías MV,Hessle C,Telemo E,Midtvedt T,Hanson LA,Wold AE

    更新日期:1999-05-01 00:00:00

  • Endogenous glucocorticoids attenuate Shiga toxin-2-induced toxicity in a mouse model of haemolytic uraemic syndrome.

    abstract::The concept that during an immune challenge the release of glucocorticoids (GC) provides feedback inhibition on evolving immune responses has been drawn primarily from studies of autoimmune and/or inflammatory processes in animal models. The epidemic form of haemolytic uraemic syndrome (HUS) occurs secondary to infect...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Gómez SA,Fernández GC,Vanzulli S,Dran G,Rubel C,Berki T,Isturiz MA,Palermo MS

    更新日期:2003-02-01 00:00:00

  • Side-effects of intravenous immune globulins.

    abstract::Intravenous immune globulin (IVIG) preparations are efficacious and safe products in use world-wide. Although rare, side-effects of IVIG may be serious, even life-threatening, and clinicians should be aware of their potential occurrence. This article summarizes most of the adverse experiences with IVIG reported in the...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Duhem C,Dicato MA,Ries F

    更新日期:1994-07-01 00:00:00

  • Contrasting activity of cytosin-guanosin dinucleotide oligonucleotides in mice with experimental colitis.

    abstract::Intestinal inflammation in inflammatory bowel disease (IBD) and experimental models of colitis is characterized by a dysregulated intestinal immune response with elevated levels of Th1 cytokines. The luminal flora has been implicated as a major factor contributing to the initiation and perpetuation of inflammation in ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Obermeier F,Dunger N,Strauch UG,Grunwald N,Herfarth H,Schölmerich J,Falk W

    更新日期:2003-11-01 00:00:00

  • LAIR-1 shedding from human fibroblast-like synoviocytes in rheumatoid arthritis following TNF-α stimulation.

    abstract::This study examined the expression of the inhibitory receptor, leucocyte-associated immunoglobulin (Ig)-like receptor-1 (LAIR-1) in fibroblast-like synoviocytes (FLS) in rheumatoid arthritis (RA) patients to investigate its potential role in the modulation of inflammatory cytokines, matrix metalloproteinases (MMPs) an...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Zhang Y,Wang S,Dong H,Yi X,Zhang J,Liu X,Zhuang R,Ding Y

    更新日期:2018-05-01 00:00:00

  • Systemic inflammation in acute intermittent porphyria: a case-control study.

    abstract::This study aimed to examine whether acute intermittent porphyria (AIP) is associated with systemic inflammation and whether the inflammation correlates with disease activity. A case-control study with 50 AIP cases and age-, sex- and place of residence-matched controls was performed. Plasma cytokines, insulin and C-pep...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Storjord E,Dahl JA,Landsem A,Fure H,Ludviksen JK,Goldbeck-Wood S,Karlsen BO,Berg KS,Mollnes TE,W Nielsen E,Brekke OL

    更新日期:2017-03-01 00:00:00

  • Recombinant Mycobacterium bovis bacillus Calmette-Guérin (BCG) expressing mouse IL-18 augments Th1 immunity and macrophage cytotoxicity.

    abstract::Interleukin-18 (IL-18) has been demonstrated to synergize with BCG for induction of a T-helper-type 1 (Th1) immune response. Since successful treatment of superficial bladder cancer with BCG requires proper induction of Th1 immunity, we have developed a recombinant (r) BCG strain that functionally secretes mouse (m) I...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Luo Y,Yamada H,Chen X,Ryan AA,Evanoff DP,Triccas JA,O'Donnell MA

    更新日期:2004-07-01 00:00:00

  • Blunted expression of miR-199a-5p in regulatory T cells of patients with chronic obstructive pulmonary disease compared to unaffected smokers.

    abstract::Chronic obstructive pulmonary disease (COPD) is characterized by an abnormal regulatory T cell (T(reg)) response and increases in T helper type 1 (Th1) and Th17 cell responses. It is unclear if dysregulation of microRNAs (miRNA) within T(reg) cells contributes to the abnormal inflammatory response in COPD. In this stu...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Chatila WM,Criner GJ,Hancock WW,Akimova T,Moldover B,Chang JK,Cornwell W,Santerre M,Rogers TJ

    更新日期:2014-07-01 00:00:00

  • Abnormalities in subset distribution, activation, and differentiation of T cells isolated from large intestine biopsies in HIV infection. The Berlin Diarrhoea/Wasting Syndrome Study Group.

    abstract::Intestinal T cells have a unique state of activation and differentiation which might specifically affect or be affected by HIV infection. Lymphocyte subsets in the peripheral blood are well characterized, but our knowledge about intestinal lymphocytes in HIV infection is incomplete. We therefore analysed lymphocytes i...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Schneider T,Ullrich R,Bergs C,Schmidt W,Riecken EO,Zeitz M

    更新日期:1994-03-01 00:00:00

  • beta7 Integrin expression is not required for the localization of T cells to the intestine and colitis pathogenesis.

    abstract::beta7 Integrins have been shown to have an important role in the localization of T cells to the intestine. Utilizing two different experimental mouse models of inflammatory bowel disease (IBD), this study was undertaken to determine if beta7 integrin expression is critical for T cell localization to the intestine and ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Sydora BC,Wagner N,Lohler J,Yakoub G,Kronenberg M,Muller W,Aranda R

    更新日期:2002-07-01 00:00:00