Abstract:
:Capsular polysaccharide from Actinobacillus actinomycetemcomitans Y4 (Y4 CP) induces bone resorption in a mouse organ culture system and osteoclast formation in mouse bone marrow cultures, as reported in previous studies. We also found that Y4 CP inhibits the release of interleukin (IL)-6 and IL-8 from human gingival fibroblast (HGF). Thus Y4 CP induces various responses in localized tissue and leads to the secretion of several cytokines. However, the effects of Y4 CP on human monocytes/macrophages are still unclear. In this study, THP-1 cells, which are a human monocytic cell line, were stimulated with Y4 CP, and we measured gene expression in inflammatory cytokine and signal transduction pathways. IL-1beta and tumour necrosis factor (TNF)-alpha mRNA were induced from Y4 CP-treated THP-1 cells. IL-1beta mRNA expression was increased according to the dose of Y4 CP, and in a time-dependent manner. IL-1beta mRNA expression induced by Y4 CP (100 microg/ml) was approximately 7- to 10-fold greater than that in the control by real-time PCR analysis. Furthermore, neither PD98059, a specific inhibitor of extracellular signal-regulated kinase nor SB203580, a specific inhibitor of p38 kinase prevented the IL-1beta expression induced by Y4 CP. However, JNK Inhibitor II, a specific inhibitor of c-Jun N-terminal kinase (JNK) prevented the IL-1beta mRNA expression induced by Y4 CP in a concentration-dependent manner. These results indicate that Y4 CP-mediated JNK pathways play an important role in the regulation of IL-1beta mRNA. Therefore, Y4 CP-transduced signals for IL-1beta induction in the antibacterial action of macrophages may provide a therapeutic strategy for periodontitis.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Iwata T,Mitani A,Ishihara Y,Tanaka S,Yamamoto G,Kikuchi T,Naganawa T,Matsumura Y,Suga T,Koide M,Sobue T,Suzuki T,Noguchi Tdoi
10.1111/j.1365-2249.2005.02836.xsubject
Has Abstractpub_date
2005-08-01 00:00:00pages
261-9issue
2eissn
0009-9104issn
1365-2249pii
CEI2836journal_volume
141pub_type
杂志文章abstract::The hydrodynamic (gel filtration and sedimentation) properties of an isolated monoclonal IgM-K cryoglobulin (McE.) and five non-cryoglobulin cold-soluble proteins, as well as their constituent monomeric subunits and (Fc)5mu and Fabmu fragments, are compared under both native and partially denaturing conditions. It is ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::A method enabling direct enumeration of mouse T and B cells from cytocentrifuged cell smears is described. The acid alpha-naphthyl acetate esterase (ANAE) staining was used as marker for T cells. The distribution of the ANAE marker-carrying lymphocytes closely followed the percentual distribution of T cells in spleen ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1976-12-01 00:00:00
abstract::The interaction of cationic proteins with IgG, IgA and IgM were investigated by solid phase radioimmunoassay. All these immunoglobulins showed avid binding, IgM giving the strongest reaction, followed by IgA and then IgG. Fc fragments of IgG gave binding, but F(ab')2 fragments from the three main Ig classes did not, s...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1987-05-01 00:00:00
abstract::The anti-schistosomular humoral responses of guinea-pigs vaccinated with radiation-attenuated cercariae of Schistosoma mansoni have been investigated in vitro. The sera of vaccinated animals contain schistosomulicidal complement-fixing antibodies which peak in titre at week 5 after vaccination and predominantly consis...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1987-02-01 00:00:00
abstract::We investigated the clearance kinetics and tissue distribution of different sized IgA in normal and macrophage-depleted rats. Rats were injected iv with liposomes containing dichloromethylene diphosphonate (DMDP). DMDP treatment resulted in complete depletion of liver macrophages 24-48 h after administration. Normal a...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1991.tb05693.x
更新日期:1991-07-01 00:00:00
abstract::Human cartilage gp-39 (HC gp-39) is a well-known autoantigen in rheumatoid arthritis (RA). However, the exact localization, fluctuation and function of HC gp-39 in RA are unknown. Therefore, using a glucose-6-phosphate isomerase (GPI)-induced model of arthritis, we investigated these aspects of HC gp-39 in arthritis. ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.12349
更新日期:2014-08-01 00:00:00
abstract::Chemotherapy has, besides the beneficial effects, several adverse effects. Suppression of the immune system is one of the most important problems. Infections caused by encapsulated bacteria like Streptococcus pneumoniae are responsible for a major part of infectious problems during and after treatment. The splenic mar...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2003.02044.x
更新日期:2003-01-01 00:00:00
abstract::Two models of murine graft-versus-host disease (GVHD) were studied with respect to autoantibody production and development of systemic lupus erythematosus (SLE) like disease. One model was induced by injection of (B10.A(4R) x B10.A(2R]F1 mice with parental (B10.A(4R] spleen and lymph node cells (groups I GVHD), the ot...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1990.tb05287.x
更新日期:1990-07-01 00:00:00
abstract::Expression of CD4 or CD8 on the cell surface is an important guide for discriminating the immunological functions of T cells. However, a minor T cell subset, which lacks both CD4 and CD8 molecules but bears the usual form of T cell receptor (TCR) alpha beta (CD4-CD8-TCR alpha beta+ T cells), has recently been found no...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1991.tb05761.x
更新日期:1991-09-01 00:00:00
abstract::The Km for AMP for 5' nucleotidase was increased in lymphocytes from patients with common variable (CVH) and sex linked (XLH) hypogammaglobulinaemia and from patients with chronic lymphatic leukaemia (CLL): the Vmax of the latter was low. The kinetic constants for cord blood lymphocytes are similar to adults and the K...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
abstract::Transmembrane activator and calcium modulator and cyclophilin ligand interactor-immunoglobulin (TACI-Ig) is a human fusion protein that binds and neutralizes both B lymphocyte stimulator (BLyS), a cytokine shown to be a key regulator of B cell maturation, proliferation and survival, and a proliferation-inducing ligand...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2010.04293.x
更新日期:2011-02-01 00:00:00
abstract::We examined the role of IL-12 in host resistance to Cryptococcus neoformans using a murine model of pulmonary and disseminated infection. In this model, mice were infected intratracheally with viable yeast cells. Mice untreated with IL-12 allowed an uncontrolled multiplication of yeast cells in the lung with infiltrat...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1996.14723.x
更新日期:1996-05-01 00:00:00
abstract::Monoclonal antibodies were produced using mice immunized with the human promyeloid cell line HL60. Two antibodies are described which identify antigens selectively expressed by myeloid cells. Studies using normal bone marrow and myeloid leukaemia cells demonstrated that one of these antibodies (AGF4.48) identifies an ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-11-01 00:00:00
abstract::Two cationic proteins, a neutral phosphatase (NP-tase) and a 70-kD protein (p70) were isolated from Staphylococcus aureus by ion exchange chromatography. We compared their properties to those of the well established B cell mitogen of whole, fixed Staph. aureus strain Cowan I cells (SAC). Both purified proteins were ab...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1995.tb03714.x
更新日期:1995-06-01 00:00:00
abstract::Chronic bone infection is associated with bone resorption. From animal studies, CD3/CD28-activated T cells are known to enhance osteoclastogenesis and bone resorption. Because CD28 is expressed constitutively on T cells and its expression is down-regulated by chronic exposure to the inflammatory environment, we charac...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.12245
更新日期:2014-04-01 00:00:00
abstract::Small molecule metabolites that are produced or altered by host-associated microbial communities are emerging as significant immune response modifiers. However, there is a key gap in our knowledge of how oral microbial metabolites affect the immune response. Here, we examined the effects of metabolites from five bacte...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.12943
更新日期:2017-06-01 00:00:00
abstract::Lipoarabinomannan (LAM), purified from the cell walls of Mycobacterium leprae and M. tuberculosis, is a potent inhibitor of interferon-gamma (IFN-gamma) mediated activation of macrophages. The capability of LAM to inhibit IFN-gamma activation of macrophages in vitro was dose dependent and required a 24-h pre-exposure....
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1990.tb06454.x
更新日期:1990-04-01 00:00:00
abstract::Immunoblotting of platelets that have been subjected to SDS-PAGE has revealed that sera from normal individuals contain IgG which binds to many platelet components. This binding was seen with autologous and heterologous platelets using serum of males and of nulliparous females who had not received blood transfusions. ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1990.tb08097.x
更新日期:1990-03-01 00:00:00
abstract::Increased serum cytokine levels have been reported in patients with autoimmune thyroid disease, but less is known about their levels in patients with Graves' ophthalmopathy (GO). It is not known whether GO is a cell-mediated or humoral autoimmune disease. We investigated whether serum cytokines are elevated in GO pati...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2000.01335.x
更新日期:2000-09-01 00:00:00
abstract::Proliferation of mesangial cells is a hallmark of glomerular disease, and understanding the regulatory mechanisms is critically important. The purpose of this study was to examine the relationship between mesangial cell proliferation and phosphorylated signal transducer and activator of transcription (STAT) 3 and to d...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2006.03073.x
更新日期:2006-05-01 00:00:00
abstract::Interferons play an important, but incompletely understood role in HIV-related disease. We investigated the effect of HAART on plasma levels of IFN-alpha, IFN-gamma, neopterin and interferon-inducible protein 10 (IP-10) in 41 HIV-infected patients during 78 weeks of therapy. At baseline HIV-infected patients had raise...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2000.01144.x
更新日期:2000-03-01 00:00:00
abstract::The emerging role of interleukin (IL)-17 as a hallmark proinflammatory cytokine of the adaptive immune system, produced primarily by a new T helper cell subset termed 'Th17', has received considerable attention. Differentiation of Th17 cells is driven by the simultaneous presence of transforming growth factor-beta and...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2249.2009.03944.x
更新日期:2009-08-01 00:00:00
abstract::A technique is described which shows how to obtain a suspension of only progressively motile spermatozoa, from semen, by active penetration into an overlying buffer layer. About 60% of the number of motile spermatozoa in the original semen sample can be obtained as an output in the removed buffer layer. With this meth...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::Chronic pulmonary infection is known to constitute a major complication of cystic fibrosis (CF). In order to determine whether a defect of antibody formation is associated with this clinical finding, we have investigated antibody production on the cellular level by using a haemolytic plaque forming cell (PFC) assay. T...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract::This study examined the expression of the inhibitory receptor, leucocyte-associated immunoglobulin (Ig)-like receptor-1 (LAIR-1) in fibroblast-like synoviocytes (FLS) in rheumatoid arthritis (RA) patients to investigate its potential role in the modulation of inflammatory cytokines, matrix metalloproteinases (MMPs) an...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.13100
更新日期:2018-05-01 00:00:00
abstract::Recent studies have suggested that a high percentage of Epstein-Barr virus (EBV)-infected lymphocytes in peripheral blood of patients with chronic, active EBV infection (CAEBV) is of T cell origin. Although T cells are expanded oligoclonally in CAEBV, it is not clear whether the restricted diversity of T cells arise f...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2003.02270.x
更新日期:2003-10-01 00:00:00
abstract::Amino acid residues involved in the peptide binding groove of HLA-DRB1 alleles were examined in three Nigerian ethnic groups with leprosy (n = 287) and 170 controls to determine the role of DRB1 alleles in disease outcome with Mycobacterium leprae. Nine positively charged motifs and two others with neutral charge to t...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1999.01034.x
更新日期:1999-10-01 00:00:00
abstract::In vitro lymphocyte function of 13 patients with selective IgA deficiency was studied. IgG, IgA and IgM secretion by pokeweed mitogen-stimulated peripheral blood lymphocytes from normal donors and IgA deficient patients was measured by an enzyme-linked immunosorbent assay. Addition of concanavalin A or hydrocortisone ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-08-01 00:00:00
abstract::We found that IgA is predominant among the immune deposits in the renal glomeruli of mice infected with Schistosoma mansoni, and thus conducted an analysis of the deposition of different immunoglobulin isotypes in the glomeruli throughout the course of infection in mice. Both immunofluorescent and immunoperoxidase met...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::Recently, naturally occurring antibodies to IFN-alpha were discovered in a few systemic lupus erythematosus (SLE) and cancer patients; however, in most patients monitored for anti-IFN antibodies before treatment, no antibodies were found. In an attempt to explain the 'IFN-blocking effect' that we observed in all serum...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1990.tb05403.x
更新日期:1990-10-01 00:00:00