Abstract：
:Chronic bone infection is associated with bone resorption. From animal studies, CD3/CD28-activated T cells are known to enhance osteoclastogenesis and bone resorption. Because CD28 is expressed constitutively on T cells and its expression is down-regulated by chronic exposure to the inflammatory environment, we characterized co-stimulatory molecule expression on T cells from chronically infected patients. We used cytofluorometric techniques to phenotypically characterize T cells, its co-stimulatory molecules and perforin secretion from infected and non-infected human bones. Chronic bone infection was defined as infection lasting for more than a month. We show a higher T cell activation [human leucocyte antigen D-related (HLA-DR⁺)] in infected compared to non-infected bones: median being 16 versus 7%, P = 0·009 for CD4 T cells, and 33 versus 15%, P = 0·038 for CD8 T cells, respectively. However, T cell proliferation (Ki67⁺) was lower for CD8 T cells in infected bones: 26 versus 34%, P = 0·045. In contrast, we detected no difference in apoptosis and regulatory T cells. In infected bone, we found higher CD28-negative CD4⁺ T cells compared to non-infected bone: 20 versus 8%, respectively (P = 0·005); this T cell subset had higher CD11b expression and perforin secretion. Chronically infected human bones are characterized by an increase of CD28-negative CD4⁺ T cells, indicating long-term activated cells with cytotoxic ability. Therefore, this alteration of co-stimulatory molecules may modify interactions with osteoclasts and impact bone resorption.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Kumar G,Roger PM,Ticchioni M,Trojani C,Bernard de Dompsur R,Bronsard N,Carles M,Bernard Edoi
10.1111/cei.12245subject
Has Abstractpub_date
2014-04-01 00:00:00pages
49-57issue
1eissn
0009-9104issn
1365-2249journal_volume
176pub_type
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journal_title：Clinical and experimental immunology
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pub_type： 杂志文章
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journal_title：Clinical and experimental immunology
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pub_type： 杂志文章
doi：
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journal_title：Clinical and experimental immunology
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doi：
更新日期：1983-11-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：1983-09-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
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doi：
更新日期：1979-07-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：1981-05-01 00:00:00
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journal_title：Clinical and experimental immunology
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更新日期：1985-07-01 00:00:00
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