Abstract:
:In order to investigate the T cell cytokine profile during age-dependent maturation of the immune response, we evaluated the cytokine expression of CD4+ and CD8+ circulating cells by flow cytometric single-cell analysis after non-specific stimulation in vitro in different age groups of normal individuals, from cord blood to adulthood. Moreover, we correlated these lymphocyte cytokine patterns with the expression/release of CD30, a member of the tumour necrosis factor (TNF) receptor superfamily, which has been suggested to be related to the T helper/cytotoxic (Th(c))2-type immune responses, in order to verify this association in vivo, in non-pathological conditions. The results showed a progressive increase of circulating Th(c)1-type, interferon-gamma (IFN-gamma)- and/or IL-2-producing T cells along with ageing and, conversely, a stable number, although higher than in cord blood samples, of CD4+/IL-4+ T cells in the post-natal groups. In addition, serum levels of soluble CD30 (sCD30) and numbers of circulating CD4+/CD30+ and CD8+/CD30+ T cells were significantly higher in children aged < 5 years in comparison with those found either in cord blood or in blood from both older children and adults. These data support the concept of a progressive polarization of the Th(c) cell cytokine profile towards the Th(c)1 pattern during age-dependent maturation of the immune response. Moreover, the peak of CD30 expression/release in early infancy before the Th(c)1 shifting occurs, although not associated with a significant increase of circulating IL-4+ T cells, raises the question of the possible relationship in vivo between CD30 and Th(c)2-type immune responses.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Krampera M,Vinante F,Tavecchia L,Morosato L,Chilosi M,Romagnani S,Zanolin ME,Pizzolo Gdoi
10.1046/j.1365-2249.1999.00977.xsubject
Has Abstractpub_date
1999-08-01 00:00:00pages
291-7issue
2eissn
0009-9104issn
1365-2249pii
cei977journal_volume
117pub_type
杂志文章abstract::Sensorineural hearing loss is a common problem in the otolaryngologist's practice, with autoimmune disease of the inner ear being one possible cause. The restoration of auditory function in some patients following immunosuppressive therapy has created a desire to define and understand this disease better. Because of t...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1994.tb06591.x
更新日期:1994-07-01 00:00:00
abstract::Cytokines have been extensively studied in coeliac disease, but cytokine release related to exposure to gluten and associated symptoms has only recently been described. Prominent, early elevations in serum interleukin (IL)-2 after gluten support a central role for T cell activation in the clinical reactions to gluten ...
journal_title:Clinical and experimental immunology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1111/cei.13369
更新日期:2020-01-01 00:00:00
abstract::Protein kinases mediate protein phosphorylation, which is a fundamental component of cell signalling, with crucial roles in most signal transduction cascades: from controlling cell growth and proliferation to the initiation and regulation of immunological responses. Aberrant kinase activity is implicated in an increas...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章,评审
doi:10.1111/cei.12248
更新日期:2014-04-01 00:00:00
abstract::In this study, we aimed to explore the effects of imatinib on the proliferation of rheumatoid arthritis synovial cell (RA-FLS) and inflammatory responses by regulating CSF1R. Differential genes were screened via microarray analysis, followed by being analysed through the weighted co-expression network (WGCNA) network,...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.13220
更新日期:2019-02-01 00:00:00
abstract::Complement activation may play an important role in renal injury associated with glomerular deposition of IgA immune complexes. The ability of naturally occurring human IgA immune complexes (IgA-IC) and covalently cross-linked human IgA oligomers (X-IgA) to activate complement were examined in vitro and in vivo. Large...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1988-09-01 00:00:00
abstract::Synovial tissues from fifteen patients with psoriatic arthritis were investigated with direct immunofluorescence staining for immunoglobulins (IgG, IgA, and IgM) and from eigth patients for complement component C3. As control groups, there were synovial tissues from seven patients with seropositive rheumatoid arthriti...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1977-09-01 00:00:00
abstract::The role of suppressive anti-retroviral therapy (ART) in eliciting restoration of dysregulated immune function remains unclear in HIV-1 infection. Also, due to tailoring of therapeutic regimens towards HIV-1, this possible impairment of therapy may be even more pronounced in HIV-2 and dual (HIV-D) infection. Thus, we ...
journal_title:Clinical and experimental immunology
pub_type: 临床试验,杂志文章
doi:10.1111/cei.13342
更新日期:2019-11-01 00:00:00
abstract::Increasing evidence has demonstrated that the immune system closely interacts with other physiological systems, whose communications are mediated by circulating cytokines and hormones. The aim of our study was to test whether the number and cytolytic activity of NK cells in a group of relatively healthy Italian nonage...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1999.00855.x
更新日期:1999-04-01 00:00:00
abstract::Biologically significant levels of IL-2 activity were produced by isolated lamina propria mononuclear cells (LPMC) from normal intestine (n = 12), cancer-bearing colons (n = 35) and inflammatory bowel disease (IBD) affected tissue (n = 12). The levels of IL-2 produced were similar for all three sources of LPMC (normal...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1992.tb03052.x
更新日期:1992-04-01 00:00:00
abstract::Fifty sera which gave ring-like nuclear staining in immunofluorescence on rat liver tissue sections were characterized. Using immunoprecipitation and Western blotting we showed that 10 of the sera contained antibodies to 200 kD polypeptide(s) of nuclear envelope. Clinical and biological data were available for nine of...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::Frozen sections of control and coeliac jejunal mucosa were examined for HLA-DR staining. In general the staining pattern in the controls and treated coeliac patients was a patchy subapical pattern on villous tips. Untreated coeliac patients gave a different pattern with staining extending to the crypt enterocytes. Glu...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::The pattern of T cell receptor V gene expression by lymphocytes from rheumatoid synovial fluid and paired peripheral blood samples was compared using a polymerase chain reaction (PCR)-based assay. Eight rheumatoid arthritis (RA) patients who had varying durations of disease (from 2 to 20 years) were studied. In all pa...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1992.tb05865.x
更新日期:1992-12-01 00:00:00
abstract::We studied the interaction of two different forms of immune glomerular damage occurring simultaneously: anti-glomerular basement membrane (GBM) antibody fixation and immune elimination of bovine serum albumin (BSA). 125I-radiolabelled BSA anti-BSA immune complexes, formed in response to a single small intravenous dose...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1979-03-01 00:00:00
abstract::A rabbit antiserum has been prepared using peripheral mononuclear cells from a patient with common variable hypogammaglobulinaemia (CVH) who had demonstrable suppressor cells in vitro. This antiserum (anti-Ck), after absorption with pooled normal glass-non-adherent cells and platelets, showed selective reactivity with...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1981-12-01 00:00:00
abstract::Streptococcus pneumoniae infection may result in asymptomatic carriage, mucosal or invasive disease. We hypothesize that self-limiting or fatal disease outcome follows infection with S. pneumoniae differential activation of the host immune response. BALB/c and C57BL/6 mice were inoculated intranasally with S. pneumoni...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2003.02261.x
更新日期:2003-10-01 00:00:00
abstract::The administration of the polyclonal activator HgCl2 (1 mg/kg i.p.) to Brown Norway (BN) rats on days 0, 2, 4 and 7 resulted in the cyclical production of anti-glomerular basement membrane (GBM) antibodies, the first peak of which occurred at day 14 with a smaller peak at day 26. Glomerular anti-GBM antibody levels we...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract::An altered expression pattern of adhesion molecules (AM) on the surface of immune cells is a premise for their extravasation into the central nervous system (CNS) and the formation of acute brain lesions in multiple sclerosis (MS). We evaluated the impact of glatiramer acetate (GA) on cell-bound and soluble AM in the ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.12125
更新日期:2013-09-01 00:00:00
abstract::The expression of guinea-pig major histocompatibility antigens (class I and II) has been investigated on guinea-pig epididymal spermatozoa. Specific alloantisera (anti-B1, anti-B3, anti-Ia2,4 and anti-Ia1,3,7) were obtained by cross-immunization of strains 2, 13 and BIO-AD animals with relevant spleen cell membranes. ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1984-09-01 00:00:00
abstract::A new antimitochondrial antibody (AMA) against an outer membrane associated antigen on liver mitochondria was detected by ELISA in sera from patients with primary biliary cirrhosis (PBC). This antibody was named anti-M9. There is evidence that it is a partial organ-specific antibody as shown by absorption studies usin...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::Human bronchoalveolar lavage (BAL) macrophages were obtained from normal human volunteers and infected with an AIDS-associated strain of Mycobacterium avium. Infected cells were exposed to purified envelope glycoprotein (gp120) from HIV-1 or to the recombinant non-glycosylated gp120 fragments PBI-RF and PBI-IIIB. Nati...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1994.tb06617.x
更新日期:1994-10-01 00:00:00
abstract::A phenotypic and functional analysis of the circulating natural killer (NK) cell population was carried out in a series of patients with hairy cell leukaemia (HCL). The overall mean NK activity of both the mononuclear and T cell fractions was reduced compared to that of normal controls (466 lytic units (lu) v 573 lu a...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::Pneumococcal infections cause a large global health burden, and the search for serotype-independent vaccines continues. Existing conjugate vaccines reduce nasopharyngeal colonization by target serotypes. Such mucosal effects of novel antigens may similarly be important. CD4+ Th17 cell-dependent, antibody-independent r...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.13225
更新日期:2019-02-01 00:00:00
abstract::It has previously been shown that murine sarcoma cells express high-affinity IL-4 receptors (IL-4R) which are internalized after binding to the ligand (Puri et al., Cancer Res 1991; 51:3011-7). We have also reported that human renal cell carcinoma cells express high-affinity IL-4R, and IL-4 inhibits tumour growth in v...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1994.tb06029.x
更新日期:1994-01-01 00:00:00
abstract::Immune complexes (IC) were detected and isolated from the serum of Brown Norway (BN), (Lewis x BN)F1, and Lewis rats bearing a Moloney sarcoma (MST). IC were isolated from the serum of individual rats employing a system of G-200 chromatography and passage through a heavy chain specific anti-rat IgG Immunoadsorbent. Ig...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-04-01 00:00:00
abstract::The capacity of peripheral blood mononuclear (PBM) cells from patients with leprosy to generate immunoglobulin-secreting cells in response to pokeweed mitogen (PWM) was evaluated by a reverse haemolytic plaque forming cell (PFC) assay. The PFC responses of PBM cells from patients with lepromatous (Lpr) leprosy were si...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00
abstract::Although Mycobacterium leprae shares many protein antigens with other mycobacterial species, there is a degree of specificity in the T cell response to the organism. This is evident in the failure of cross-protection between mycobacterial species and the specific unresponsiveness to M. leprae in lepromatous leprosy pa...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1995.tb06636.x
更新日期:1995-10-01 00:00:00
abstract::In order to evaluate the impact of induction and increase target antigen expression on immunotoxin potency, we measured the potentiating effect of recombinant immune interferon-gamma (rIFN-gamma) on the cytotoxicity of an anti HLA-DR ricin A-chain immunotoxin (2G5 RTA-IT) on the myeloid cell line ML-3. After 48 h of i...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1990.tb05429.x
更新日期:1990-11-01 00:00:00
abstract::Cellular sensitization to rabbit liver specific lipoprotein (RLSP) has been investigated using a lymphocyte transformation test in patients with chronic active hepatitis (CAH). A stimulation index greater than 2 was recorded in twenty out of twenty-five cases (eight of ten HBsAg positive and twelve of fiteen HBsAg neg...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1979-11-01 00:00:00
abstract::Granulocyte colony-stimulating factor (G-CSF) administration in vivo has been shown to improve the defence mechanisms against infection by different microbes. Here we evaluated a possible protective role of this molecule in a mouse model of mycobacterial infection. The administration of recombinant G-CSF promoted an e...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2001.01552.x
更新日期:2001-05-01 00:00:00
abstract::Previous studies show that endoplasmic reticulum-associated aminopeptidase (ERAP1/ERAP2) and runt-related transcription factor 3 (RUNX3) gene polymorphisms are associated with AS (ankylosing spondylitis) in European Caucasians. However, contradictory results were reported in different Asian populations. The purpose of...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.13121
更新日期:2018-07-01 00:00:00