Abstract：
:Donor-specific CTL present within the cardiac allograft during a rejection episode are distinct from those that populate the cardiac allograft in the absence of rejection. Whereas the former generally have a high avidity for donor cells, the latter mainly have a low avidity for donor cells. This observation made us reason that high-avidity CTL are implicated in transplant rejection, whereas low-avidity CTL are not. In the present study, we analyse whether both CTL subsets were distinct with respect to their IL-2, IL-4, IL-6 and interferon-gamma (IFN-gamma) secretion pattern. CTL clones with either a high or a low avidity for donor antigens were stimulated with donor cells, third party cells, or immobilized anti-CD3 MoAb and the amount of cytokine released was measured. High- and low-avidity CTL clones were found to differ with respect to their IFN-gamma production profile. Stimulation with donor cells resulted in IFN-gamma secretion by high-avidity CTL clones, but not by low-avidity CTL clones. CD3 stimulation, in contrast, led to secretion of equivalent amounts of IFN-gamma by both CTL subsets. These observations indicate that low-avidity CTL are fully capable of producing IFN-gamma, but, in contrast to high avidity CTL, fail to do so when they encounter donor cells. As IFN-gamma favours the occurrence of transplant rejection, this observation emphasizes the relevance of high-avidity CTL in the rejection process. Additionally, the data show that the cytokine production profile of CTL depends on the nature of the stimulus.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Van Emmerik NE,Daane CR,Knoop CJ,Hesse C,Vaessen LM,Balk AH,Mochtar B,Claas FH,Weimar Wdoi
10.1046/j.1365-2249.1997.4441458.xsubject
Has Abstractpub_date
1997-12-01 00:00:00pages
447-53issue
3eissn
0009-9104issn
1365-2249journal_volume
110pub_type
杂志文章abstract：:The in vitro effect of the anti-thyroid drugs (ATD), propylthiouracil (PTU) and methimazole (MMI) on blastogenesis of peripheral blood mononuclear cells (PBMC) from healthy subjects was studied in 72 hr PHA stimulated cultures. PTU in therapeutic concentrations (10 micrograms/ml) suppressed blastogenesis only when add...
journal_title：Clinical and experimental immunology
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doi：
更新日期：1982-10-01 00:00:00
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journal_title：Clinical and experimental immunology
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更新日期：1987-03-01 00:00:00
abstract：:Insufficient stimulatory capacities of autologous dendritic cells (DC) may contribute in part to impaired T cell stimulation and therefore viral persistence in patients with chronic hepatitis B virus (HBV) infection. In order to characterize the antigen presenting functions of DC from chronic HBV carriers and controls...
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
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doi：
更新日期：1986-01-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1999.00986.x
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journal_title：Clinical and experimental immunology
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doi：
更新日期：1975-05-01 00:00:00
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-11-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-06-01 00:00:00
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
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doi：
更新日期：1985-08-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1978-11-01 00:00:00
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13142
更新日期：2018-08-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-09-01 00:00:00
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journal_title：Clinical and experimental immunology
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doi：10.1111/j.1365-2249.2004.02581.x
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