Abstract:
:The high affinity form of the human IL-2 receptor (IL-2R) has two known components, the IL-2R alpha (p55) and the IL-2R beta chain (p75). We have previously shown that recombinant IL-2 (rIL-2) could induce the expression of the alpha-chain (p55) on T cells and thymocytes, and increase this expression following suboptimal activation with concanavalin A (Con A) in combination with IL-2. An increase in the accumulation of IL-2R alpha-specific mRNA induced by rIL-2 in T cells and thymocytes had also been documented. We report here that the expression of IL-2R beta on the cell surface can be demonstrated on human thymocytes by the binding of Mik beta1, a MoAb directed against an epitope of the beta-chain. The IL-2R beta chain is constitutively expressed on freshly isolated thymocytes; this expression can be increased in thymocytes activated with Con A in combination with IL-2 or tetradecanoylphorbol 13-acetate (TPA). Blocking the formation of high affinity receptors with a MoAb directed against the alpha-chain of the receptor results in an increase in the display of IL-2R beta as evidenced by binding of MoAb Mik beta1. The accumulation of IL-2R-beta-specific mRNA is observed in freshly isolated thymocytes and it is increased in thymocytes cultured with rIL-2 alone, with Con A, and further enhanced by the addition of rIL-2 in combination with Con A or with TPA. Cyclosporine (CsA), which inhibits the accumulation of lymphokine-specific mRNA of thymocytes, does not inhibit the induction of the accumulation of IL-2R beta-specific mRNA. This is analogous to its effect on the expression of the alpha-chain (p55), and the accumulation of alpha-chain-specific mRNA.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Reem GH,Han X,Marcelli Adoi
10.1111/j.1365-2249.1992.tb06429.xsubject
Has Abstractpub_date
1992-01-01 00:00:00pages
150-6issue
1eissn
0009-9104issn
1365-2249journal_volume
87pub_type
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更新日期:1982-11-01 00:00:00
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doi:
更新日期:1986-01-01 00:00:00
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journal_title:Clinical and experimental immunology
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doi:
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journal_title:Clinical and experimental immunology
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doi:
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journal_title:Clinical and experimental immunology
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pub_type: 杂志文章
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