Abstract：
:Solubilization of an immune precipitate by serum is a complement function mediated by the alternative pathway and enhanced by the classical pathway--it therefore provides the basis of a simple quantitative assessment of the integrity of complement function. Using a preformed radiolabelled precipitate of BSA-alpha BSA Ab, the solubilization capacity of serial sera from 75 patients with various immune complex diseases or diseases associated with hypocomplementaemia was investigated to correlate this assay of complement function prospectively with disease activity and with measurements of circulating immune complexes (CIC). Reduction in solubilization, defined by more than 25% of values in a given patient being below the normal range, was found in 11 of 12 patients with active SLE, two of 19 patients with active systemic vasculitis, three of three patients with post-streptococcal glomerulonephritis and in three of six patients with nephrotic syndrome due to other types of nephritis. In serial studies, solubilization correlated with disease activity in patients with SLE (P less than 0.005), systemic vasculitis (P less than 0.05) and post-streptococcal glomerulonephritis (P less than 0.05). CIC were found more frequently than abnormalities in solubilization; however, the solubilization assay identified a population of patients with CIC more likely to have active disease. This simple assay of complement function provides data on an aspect of immune complex disease not readily apparent from standard estimations of circulating immune complexes, and appears to be a better measure of their potential phlogistic effects.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Schifferli JA,Morris SM,Dash A,Peters DKsubject
Has Abstractpub_date
1981-12-01 00:00:00pages
557-64issue
3eissn
0009-9104issn
1365-2249journal_volume
46pub_type
杂志文章abstract：:Epstein-Barr virus (EBV) is a persistent virus with oncogenic capacity that has been implicated in the development of aggressive B cell lymphomas, primarily in immunosuppressed individuals, although it can be present in immunocompetent individuals. Changes in the function and clonal diversity of T lymphocytes might be...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12682
更新日期：2015-11-01 00:00:00
abstract：:Five out of 51 sera (10%) from patients with progressive systemic sclerosis reacted by immunoblotting with tissue concanavalin A binding glycoproteins of 50 kD and 45 kD Mr, whereas only one out of 133 control sera gave the same reaction (P less than 0.001). The antigens were localized to the microsomal fractions of t...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:Secretory IgA antibody responses such as those directed against respiratory and enteric pathogens, may be impaired in protein-calorie malnutrition. In previous studies using a rat model of the mucosal immune response to cholera toxin, as little as 2 weeks of severe protein deprivation markedly impaired mucosal anti-to...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:We describe the usefulness of a fast (48-h) limiting dilution assay (LDA) for the enumeration of human alloreactive helper T lymphocytes (HTL) in the peripheral blood, in relation to histologically defined rejection grades after heart transplantation. HTL frequencies (HTLf) in pretransplant samples varied from patient...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1999.01091.x
更新日期：1999-12-01 00:00:00
abstract：:Positive skin reactions to PPD in guinea-pigs immunized with Freund's complete adjuvant (FCA) were reversed after treatment with 10 mg/kg/day nifurtimox for 12 days. The in vitro migration of peripheral blood leucocytes from FCA-immunized guinea-pigs was inhibited with PPD, but it returned to normal values after nifur...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-12-01 00:00:00
abstract：:One hundred and sixty-two unrelated, healthy normals and 118 unrelated SLE patients were subdivided by sex, race, and disease, and each group was in Hardy-Weinberg equilibrium for autosomal codominant expression of the four CR1 alleles. There were no significant phenotypic differences between males and females (P grea...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:Induction of allograft tolerance has been considered the ultimate goal in organ transplantation. Although numerous protocols to induce allograft tolerance have been reported in mice, a chimerism-based approach through donor haematopoietic stem cell transplantation has been the only approach to date that induced allogr...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/cei.12969
更新日期：2017-08-01 00:00:00
abstract：:Lymphocytes from normal individuals with the histocompatibility antigens HLA-B8 and DR3 have impaired proliferative responses when stimulated with suboptimal concentrations of mitogens. We have previously shown that an important factor in the impaired response is a failure to produce normal quantities of interleukin-2...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1990.tb05183.x
更新日期：1990-02-01 00:00:00
abstract：:Although Mycobacterium leprae shares many protein antigens with other mycobacterial species, there is a degree of specificity in the T cell response to the organism. This is evident in the failure of cross-protection between mycobacterial species and the specific unresponsiveness to M. leprae in lepromatous leprosy pa...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1995.tb06636.x
更新日期：1995-10-01 00:00:00
abstract：:Three B cell hybridomas were produced by the fusion of spleen cells from a 5 month old MRL/Mp-lpr/lpr mouse with the myeloma cell line, NS-1. By competitive inhibition, all three monoclonal antibodies (MoAb) were specific for poly(rA) and were inhibited to a lesser extent by dDNA, nDNA, poly(rI) and poly(rC). Moreover...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:People receiving therapy with either 8-methoxypsoralen plus long wavelength ultraviolet radiation (PUVA) or medium wavelength ultraviolet radiation (UVB) have been studied to see whether their unresponsiveness to the contact sensitizer dinitrochlorobenzene (DNCB) is a form of immunological tolerance. It was confirmed ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:Silicosis is a chronic progressive granulomatous and fibrotic lung disease caused by inhaled silica. Although the causative agent is known, the pathogenesis, especially the immunologic response, is not well understood. We examined two important components of cell-mediated immune responses in the lungs of rats with sil...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
abstract：:Histamine (10(-3)-10(-8) M) inhibits PHA-induced proliferation of human peripheral blood lymphocytes (HPBL). Inhibition is detected at low concentrations of PHA but is rarely observed at high PHA concentrations. The histamine type II (H2) receptor agonists dimaprit, impromidine and 4-methylhistamine (4MH) inhibit HPBL...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:Membranous nephropathy (MN), the leading cause of nephrotic syndrome in adults, is characterized by the deposition of subepithelial immune deposits that consist mainly of immunoglobulin (Ig)G and complement. Most of the cases are primary or idiopathic (iMN), while only approximately 25% of the cases are secondary to s...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/cei.12729
更新日期：2016-02-01 00:00:00
abstract：:The immune system and the kidneys are closely linked. In health the kidneys contribute to immune homeostasis, while components of the immune system mediate many acute forms of renal disease and play a central role in progression of chronic kidney disease. A dysregulated immune system can have either direct or indirect...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/cei.13119
更新日期：2018-05-01 00:00:00
abstract：:We found that IgA is predominant among the immune deposits in the renal glomeruli of mice infected with Schistosoma mansoni, and thus conducted an analysis of the deposition of different immunoglobulin isotypes in the glomeruli throughout the course of infection in mice. Both immunofluorescent and immunoperoxidase met...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Human umbilical vein endothelial cells (HUVEC) were investigated for their ability to produce neopterin, a biochemical marker for an activated immune system. Interferon-gamma (IFN-gamma), IL-1 alpha, IL-2, IL-6, tumour necrosis factor-alpha, granulocyte/macrophage colony stimulating factor, phytohaemagglutinin and con...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1992.tb06486.x
更新日期：1992-06-01 00:00:00
abstract：:CD5 is an antigen normally found on T cells and on a minority subpopulation of B cells in fetal spleen and tonsil and on the majority of cells in B-chronic lymphocytic leukaemia. Recent reports described the occurrence of large numbers of CD5-positive B cells in the peripheral blood after bone marrow transplantation (...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:Neutrophils maintain immune homeostasis by engulfing apoptotic cells and debris. We describe the rapid activation of neutrophils after engulfing hemoglobin (Hb)-activated platelets, which are abundant in the circulation of hemolytic patients. Neutrophils from healthy individuals after engulfing Hb-activated platelets ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13310
更新日期：2019-08-01 00:00:00
abstract：:Small molecule metabolites that are produced or altered by host-associated microbial communities are emerging as significant immune response modifiers. However, there is a key gap in our knowledge of how oral microbial metabolites affect the immune response. Here, we examined the effects of metabolites from five bacte...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12943
更新日期：2017-06-01 00:00:00
abstract：:In stimulated cultures, a proportion of human T lymphocytes form multilayer rosettes with sheep red blood cells (SRBC) and they can be easily separated from common E rosettes by velocity sedimentation. We have developed a simplified method for harvesting multilayer rosettes and quantifying them. Practical applications...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-04-01 00:00:00
abstract：:Five patients with no detectable serum IgA (less than 20 mug/ml) and one patient with low serum IgA were compared to normal subjects. The number of circulating E-RFC was normal as was the lymphocyte DNA synthesis induced by PHA, Con A, and streptokinase-streptodornase. The patients had normal numbers of IgA-bearing ly...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
abstract：:Severe acute respiratory syndrome (SARS) is a new disease which has spread rapidly and widely. We wished to know whether evaluation of in vitro cytokine production could contribute to improved understanding of disease pathogenesis and to better patient management. Numbers of unstimulated and mitogen-stimulated cytokin...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2003.02391.x
更新日期：2004-03-01 00:00:00
abstract：:The anti-EBV T cell response was studied in a severe combined immunodeficiency patient (PS) who received two transplants of fetal liver cells. His peripheral blood mononuclear cells (PBMC) were incubated with EBV and cultured during 15 days. Eleven colonies were derived from the T lymphocytes causing the regression of...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：:The anti-lymphocyte autoantibody response to irradiated lethal Plasmodium berghei malaria parasites in normal mice was significantly reduced when recipients were pre-treated with splenic T cells from mice recovered from a non-lethal Plasmodium yoelii infection. Suppression was specific for the autoantibody and did not...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1983-10-01 00:00:00
abstract：:The C77G polymorphism in exon A of the human CD45 gene occurs with low frequency in healthy individuals. An enhanced frequency of C77G individuals has been reported in cohorts of patients suffering from multiple sclerosis, systemic sclerosis, autoimmune hepatitis, hepatitis C and human immunodeficiency virus (HIV)-1. ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2007.03508.x
更新日期：2007-12-01 00:00:00
abstract：:Saimiri sciureus monkeys have been successfully immunized against a human malaria parasite, Plasmodium falciparum, using soluble antigens purified from schizont and merozoite extracts. High levels of antibodies reacting with schizont and merozoite specific polypeptides of 140 and 200 kdaltons were detected in the sera...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-04-01 00:00:00
abstract：:Eosinophils from the peritoneal washings of normal rats adhered to live or formalin-fixed schistosomula of Schistosoma mansoni in vitro, in the presence of heat-inactivated serum from infected rats. Eosinophil adherence caused permeability changes in the schistosomula as revealed by 51Cr release and methylene blue upt...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-10-01 00:00:00
abstract：:Disseminated infection with virulent Mycobacterium avium in C57Bl/6 (B6) mice leads to severe lymphocyte depletion in secondary lymphoid organs. In this study, we found an up-regulation of caspase-8 activity in spleen cell extracts from M. avium 25291-infected B6 mice compared to non-infected mice. The activation of t...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2011.04385.x
更新日期：2011-06-01 00:00:00
abstract：:Peripheral large granular lymphocytes (LGL) were enumerated in normal subjects and patients with autoimmune thyroid diseases. In normal subjects, the percentage of LGL was significantly lower in women (mean +/- s.d., 17.0 +/- 3.6%; n = 35; P less than 0.05) than in men (19.5 +/- 5.3%; n = 20). In untreated patients wi...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
