当前位置： SCI文献检索 > CLINICAL AND EXPERIMENTAL IMMUNOLOGY期刊下所有文献 > T helper frequencies in peripheral blood reflect donor-directed reactivity in the graft after clinical heart transplantation.

T helper frequencies in peripheral blood reflect donor-directed reactivity in the graft after clinical heart transplantation.

Abstract：

:We describe the usefulness of a fast (48-h) limiting dilution assay (LDA) for the enumeration of human alloreactive helper T lymphocytes (HTL) in the peripheral blood, in relation to histologically defined rejection grades after heart transplantation. HTL frequencies (HTLf) in pretransplant samples varied from patient to patient, ranging from 106 to 625 HTL/106 peripheral blood mononuclear cells (PBMC). In the first week after heart transplantation (HTx), when immunosuppression was instituted, HTLf were significant lower (range 30-190 HTL/106). The level of HTL in the first week after HTx when rejection grade was 0 or 1A (ISHLT score) was considered to be the baseline frequency. This frequency did not correlate with the number of subsequent rejection episodes. During rejection (grade 3), donor-specific HTLf were increased above their baseline frequencies (P = 0.01). Expressed as percentage of baseline frequencies, HTLf increased significantly during acute rejection (AR) compared with 1-2 weeks before rejection (P = 0.003). The increase was specific, since viral infections did not result in a rise of donor-specific HTL, while also HTLf specific for third party HLA antigens were not elevated during rejection. Monitoring HTLf in peripheral blood with a shortened (48-h) assay may serve as a non-invasive method for detecting intragraft immunological reactivity. Demonstrating absence of donor-specific reactivity may limit the number of invasive endomyocardial biopsy (EMB) procedures and allow tapering of immunosuppressive treatment.

journal_name

Clin Exp Immunol

journal_title

Clinical and experimental immunology

authors

Vaessen LM,Daane CR,Maat AP,Balk AH,Claas FH,Weimar W

doi

10.1046/j.1365-2249.1999.01091.x

subject

Has Abstract

pub_date

1999-12-01 00:00:00

pages

473-9

issue

3

eissn

0009-9104

issn

1365-2249

pii

cei1091

journal_volume

118

pub_type

杂志文章

相关文献

CLINICAL AND EXPERIMENTAL IMMUNOLOGY文献大全
  • Study of antigenic sites on the asialoglycoprotein receptor recognized by autoantibodies.

    abstract：:The aim of this study was to identify the epitopes recognized by antibodies to the asialoglycoprotein receptor, a specific hepatocyte protein, from sera of patients with autoimmune hepatitis. An ELISA test was used to detect anti-asialoglycoprotein receptor antibodies in the sera of patients with autoimmune hepatitis....

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1998.00673.x

    authors： Hajoui O,Martin S,Alvarez F

    更新日期：1998-09-01 00:00:00

  • Inhibition of HIV-1-mediated syncytium formation and virus replication by the lipophosphoglycan from Leishmania donovani is due to an effect on early events in the virus life cycle.

    abstract：:Previous findings have indicated that the major surface molecule of Leishmania, lipophosphoglycan (LPG), could abrogate HIV-1-induced syncytium formation and virus replication. In the present work, we were interested in characterizing this inhibitory process. Data from a new luciferase-based semiquantitative assay for...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2001.01492.x

    authors： Genois N,Barbeau B,Olivier M,Tremblay MJ

    更新日期：2001-04-01 00:00:00

  • Eosinophilic gastroenteritis: ultrastructural evidence for a selective release of eosinophil major basic protein.

    abstract：:Ultrastructural study of mucosal eosinophils in a case of eosinophilic gastroenteritis involving stomach, duodenum and ileum showed an altered structure in ulcerated duodenal areas. The electron core density of eosinophil granules was inverted or disappeared and tubulovesicular structures occurred. Using immunogold st...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Torpier G,Colombel JF,Mathieu-Chandelier C,Capron M,Dessaint JP,Cortot A,Paris JC,Capron A

    更新日期：1988-12-01 00:00:00

  • Expression of CD45R0 (UCHL1) by CD4+ and CD8+ T cells as a sign of in vivo activation in infectious mononucleosis.

    abstract：:CD45R0 (UCHL1), a member of leucocyte common antigen family, is expressed largely on previously activated or memory T cells. We examined CD45R0 expression of T cell subpopulations in patients with Epstein-Barr virus (EBV) induced infectious mononucleosis (IMN) as a sign of in vivo activation. Consistent with the notio...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1991.tb05659.x

    authors： Miyawaki T,Kasahara Y,Kanegane H,Ohta K,Yokoi T,Yachie A,Taniguchi N

    更新日期：1991-03-01 00:00:00

  • Characterization of monoclonal antibodies directed towards the microsomal/microvillar thyroid autoantigen recognized by Hashimoto autoantibodies.

    abstract：:Monoclonal antibodies reacting with thyroid microsomes have been described. In this report, we present evidence that some of these monoclonal antibodies (MoAb) MAH C3 and perhaps MAH C6 are directed towards the thyroid microsomal antigen towards which microsomal autoantibodies from Hashimoto patients are directed. Imm...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Banga JP,Mirakian R,Hammond L,Pryce G,Bidey S,Bottazzo F,Weetman AP,McGregor AM,Roitt IM

    更新日期：1986-06-01 00:00:00

  • Effect of rituximab on B cell phenotype and serum B cell-activating factor levels in patients with thrombotic thrombocytopenic purpura.

    abstract：:Autoantibodies inhibiting the activity of the metalloproteinase, ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13), underlie the pathogenesis of thrombotic thrombocytopenic purpura (TTP). Rituximab (RTX) combined with plasma-exchange (PEX) is an effective treatment in TTP. Pa...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12472

    authors： Becerra E,Scully MA,Leandro MJ,Heelas EO,Westwood JP,De La Torre I,Cambridge G

    更新日期：2015-03-01 00:00:00

  • Antibody penetration into living cells. IV. Different effects of anti-native DNA and anti-ribonucleoprotein IgG on the cell cycle of activated T gamma cells.

    abstract：:Normal T cells bearing receptors for the Fc portion of IgG that were incubated in anti-RNP or anti-DNA at the time of activation with phytohaemagglutinin showed different effects on this activation as determined by flow cytometric analysis of acridine orange stained cells. Incubation in anti-RNP caused an arrest in th...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Alarcón-Segovia D,Llorente L

    更新日期：1983-05-01 00:00:00

  • Correlation between chemical structure and biological activities of Porphyromonas gingivalis synthetic lipopeptide derivatives.

    abstract：:We recently separated a PG1828-encoded triacylated lipoprotein (Pg-LP), composed of two palmitoyl and one pentadecanoyl groups at the N-terminal of glycerocysteine from Porphyromonas gingivalis, a periodontopathic bacteria, and found that Pg-LP exhibited definite biological activities through Toll-like receptor (TLR) ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2006.03182.x

    authors： Makimura Y,Asai Y,Taiji Y,Sugiyama A,Tamai R,Ogawa T

    更新日期：2006-10-01 00:00:00

  • Intrapulmonary concentrations of inflammatory cytokines in a mouse model of chronic respiratory infection caused by Pseudomonas aeruginosa.

    abstract：:We investigated the role of inflammatory cytokines in a mouse model of chronic Pseudomonas aeruginosa infection mimicking diffuse panbronchiolitis (DPB), and determined the effects of clarithromycin therapy on the production of these cytokines. The concentrations of IL-1beta, IL-2, IL-4, IL-5, interferon-gamma (IFN-ga...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2000.01343.x

    authors： Yanagihara K,Tomono K,Kuroki M,Kaneko Y,Sawai T,Ohno H,Miyazaki Y,Higashiyama Y,Maesaki S,Kadota J,Kohno S

    更新日期：2000-10-01 00:00:00

  • Selective CCR2-targeted macrophage depletion ameliorates experimental mesangioproliferative glomerulonephritis.

    abstract：:The CCL2/CCR2 chemokine/receptor axis directs the chemotaxis of infiltrating monocytes/macrophages and T cells and plays a pivotal role in tissue damage and fibrosis in kidney diseases. The eradication of the activated leucocytes should diminish the production of inflammatory mediators, limit tissue damage and amelior...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2008.03819.x

    authors： McIntosh LM,Barnes JL,Barnes VL,McDonald JR

    更新日期：2009-02-01 00:00:00

  • Expression of the B cell repertoire in lpr mice; abnormal expansion of a few VHJ558 germ-line genes.

    abstract：:Analysis of the VH gene repertoire of the J558 family was done in lipopolysaccharide (LPS)-stimulated resting cells and in vivo activated cells derived from C57Bl/6-lpr mice (IghCb). Using a restriction fragment length polymorphism (RFLP) based on digestion with the restriction enzyme Pstl, the expression of the subfa...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1995.tb05543.x

    authors： Alarcón-Riquelme ME,Fernández C

    更新日期：1995-02-01 00:00:00

  • Membrane characteristics of established human T- and B-cell lines. Cross-reactivity of human T-antigenic determinants with peripheral lymphocytes of non-human primates and the presence of MLC antigens on cultured T-cell lines.

    abstract：:Human T-cell lines (MOLT-4 and SOMMER-T) were injected into rabbits and monkeys (stumptail and squirrel monkeys). Rabbit anti-MOLT serum was absorbed with human liver and cultured B cells. Absorbed anti-MOLT serum was cytotoxic to lymphocytes of baboons and stumptail monkeys. Rabbit anti-MOLT and SOMMER-T sera after a...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Takada A,Babbitt H,Hayata I,Takada Y

    更新日期：1976-04-01 00:00:00

  • Correlations among cutaneous reactivity to DNCB, PHA-induced lymphocyte blastogenesis and peripheral blood E rosettes.

    abstract：:Comparisons of the results obtained in a study of fifty-two patients with genitourinary malignancies using three assays to monitor the thymus-dependent immune system (delayed cutaneous hypersensitivity to DNCB, PHA-induced lymphocyte blastogenesis, and peripheral blood E rosette-forming lymphocyte counts) yielded stat...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Catalona WJ,Tarpley JL,Potvin C,Chretien PB

    更新日期：1975-02-01 00:00:00

  • Production of interleukin (IL)-5 and IL-10 accompanies T helper cell type 1 (Th1) cytokine responses to a major thyroid self-antigen, thyroglobulin, in health and autoimmune thyroid disease.

    abstract：:Tumour necrosis factor (TNF)-alpha and interferon (IFN)-gamma exert detrimental effects in organ-specific autoimmune disease, while both destructive and protective roles have been demonstrated for interleukin (IL)-10, IL-4 and IL-5. We examined the production of these cytokines by peripheral blood mononuclear cells (P...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2006.03283.x

    authors： Nielsen CH,Hegedüs L,Rieneck K,Moeller AC,Leslie RG,Bendtzen K

    更新日期：2007-02-01 00:00:00

  • The influence of oral immunization on local and systemic immune responses to heterologous antigens.

    abstract：:Oral immunization with 25 mg/day of ovalbumin resulted in a number of changes in non-specific immune responsiveness, including increases in antibody produced to T-dependent but not T-independent antigens and the abrogation of tolerance induced by both oral and intravenous routes. The effect was transient, sex- and str...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Stokes CR,Newby TJ,Bourne FJ

    更新日期：1983-05-01 00:00:00

  • Immunostimulating activities of the novel peptidomimetic L-glutamyl-histamine.

    abstract：:An original representative of histamine-containing peptidomimetics L-glutamyl-histamine (L-Glu-Hist) was synthesized and characterized as a cytokine mimic leading to cellular responses of improved specificity. The energy-minimized 3-D conformations of L-Glu-Hist derived from its chemical structure resulted in stabiliz...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2004.02710.x

    authors： Babizhayev MA,Semiletov YA,Lul'kin YA,Sakina NL,Savel'yeva EL,Alimbarova LM,Barinskii IP

    更新日期：2005-03-01 00:00:00

  • Depression of lymphocyte response to phytohaemagglutinin in the presence of plasma from children with acute protein energy malnutrition.

    abstract：:Phytohaemagglutinin-induced lymphocyte transformation of four healthy adults and a child with kwashiorkor was performed in the presence of plasma from nineteen patients with protein energy malnutrition and a nutritionally normal child. Marked inhibition occurred in the presence of plasma from six patients who subseque...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Heyworth B,Moore DL,Brown J

    更新日期：1975-10-01 00:00:00

  • Cross-reactive antibodies between HIV-gp120 and platelet gpIIIa (CD61) in HIV-related immune thrombocytopenic purpura.

    abstract：:We have previously demonstrated that immune platelet destruction observed in an AIDS-free HIV-infected patient was associated with the presence of a cross-reactive antibody recognizing both HIV-glycoprotein (gp)120 and platelet gpIIIa (CD61). We have now investigated the presence of such antibodies in other HIV-infect...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1996.917606.x

    authors： Bettaieb A,Oksenhendler E,Duedari N,Bierling P

    更新日期：1996-01-01 00:00:00

  • Anti-cardiolipin antibodies and circulating immune complexes in type 1 diabetes mellitus: increased prevalence and relation to vascular complications.

    abstract：:Anti-cardiolipin antibodies, oxidatively modified low-density lipoproteins (oxLDL) and circulating immune complexes are humoral factors that have been linked to vascular damage. To analyse their possible role in the vascular complications in type 1 diabetes mellitus, we investigated patients with and without vascular ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1999.00788.x

    authors： Ahmed E,Nityanand S,Mustafa A,Brismar K,Lefvert AK

    更新日期：1999-02-01 00:00:00

  • Rapid intestinal ischaemia-reperfusion injury is suppressed in genetically mast cell-deficient Ws/Ws rats.

    abstract：:Ws/Ws rats have a small deletion of the c-kit gene, and are deficient in both mucosal and connective tissue-type mast cells. In this study, the role of mucosal type mast cells (MMC) in the development of intestinal ischaemia-reperfusion injury was investigated in Ws/Ws rats. Autoperfused segments of the jejunum were e...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1999.00851.x

    authors： Andoh A,Kimura T,Fukuda M,Araki Y,Fujiyama Y,Bamba T

    更新日期：1999-04-01 00:00:00

  • Piroxicam, indomethacin and aspirin action on a murine fibrosarcoma. Effects on tumour-associated and peritoneal macrophages.

    abstract：:Growth of a methylcholanthrene-induced fibrosarcoma in BALB/c mice was accompanied by an increase in the activation state of tumour-associated macrophages (TAM), as measured by their FcIgG receptor expression, phagocytic index and beta-glucuronidase levels. All of these parameters were markedly higher in TAM than in p...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1991.tb05816.x

    authors： Valdéz JC,Perdigón G

    更新日期：1991-11-01 00:00:00

  • Antibody-independent activation of the classical pathway of complement by Epstein-Barr virus.

    abstract：:A purified preparation of Epstein-Barr virus (EBV) has been shown to activate the classical complement pathway by direct interaction with the first component of complement, C1, without the intervention of antibody. No evidence was found for activation of the alternative pathway. Following classical pathway activation ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Martin H,McConnell I,Gorick B,Hughes-Jones NC

    更新日期：1987-03-01 00:00:00

  • The effect of anti-neutrophil cytoplasm autoantibodies on the signal transduction in human neutrophils.

    abstract：:The effect of anti-neutrophil cytoplasm autoantibodies (ANCA) on neutrophil activation was studied by pre-incubating neutrophils with IgG and F(ab')2 prepared from ANCA patients with Wegener's granulomatosis or microscopic polyarteritis. We measured the generation of inositol triphosphate (IP3) (a product of hydrolysi...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1991.tb05738.x

    authors： Lai KN,Lockwood CM

    更新日期：1991-09-01 00:00:00

  • Analysis of T cell subsets by monoclonal antibodies in patients with tuberculosis after in vitro stimulation with purified protein derivative of tuberculin.

    abstract：:By using OKT monoclonal antibodies; OKT3(pan T), OKT4(inducer/helper), OKT8 (suppressor/cytotoxic) and OKIa1, T lymphocyte subsets were examined in lymphocytes of patients with tuberculosis both before and after in vitro stimulation with purified protein derivative of tuberculin (PPD). In freshly obtained lymphocytes ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Shiratsuchi H,Tsuyuguchi I

    更新日期：1984-08-01 00:00:00

  • Prevalence in myositis of antibodies recognizing anti-U3 RNA probably in a novel complex with 22/25 kD protein and not fibrillarin.

    abstract：:New antibodies against a U3 snRNP, which were named anti-Myo 22/25 antibodies, were detected in four (8%) of 53 serum samples from patients with polymyositis/dermatomyositis (PM/DM) by RNA immunoprecipitation. In the protein immunoprecipitation analysis, all four serum samples precipitated 22 kDa and 25 kDa proteins, ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2001.01650.x

    authors： Kubo M,Ihn H,Kuwana M,Yamane K,Yazawa N,Tamaki K

    更新日期：2001-11-01 00:00:00

  • Hepatic expression of CCL2 in alcoholic liver disease is associated with disease severity and neutrophil infiltrates.

    abstract：:Serum levels and liver expression of CCL2 are increased in patients with alcoholic hepatitis (AH). In an experimental model of alcoholic liver disease (ALD), CCL2 was implicated in proinflammatory cytokines activation and hepatic lipid metabolism, but its role in human disease is currently unknown. In a large cohort o...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2012.04609.x

    authors： Degré D,Lemmers A,Gustot T,Ouziel R,Trépo E,Demetter P,Verset L,Quertinmont E,Vercruysse V,Le Moine O,Devière J,Moreno C

    更新日期：2012-09-01 00:00:00

  • Suppression of IL-6 biological activities by activin A and implications for inflammatory arthropathies.

    abstract：:Activin A is a cytokine whose multiple functions have yet to be fully determined. In this study, the role of proinflammatory cytokines in regulatory control of activin A production was shown in synoviocytes and chondrocytes. Additional facets of functional inflammation-related activities of activin A were also determi...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1998.00522.x

    authors： Yu EW,Dolter KE,Shao LE,Yu J

    更新日期：1998-04-01 00:00:00

  • Diabetic sera react with the glutamic acid decarboxylase molecule in a dimeric-oligomeric form.

    abstract：:Glutamic acid decarboxylase (GAD) is a major autoantigen in insulin-dependent diabetes mellitus (IDDM). This was initially identified as a 64-65 kD molecule according to migration in gels after immunoprecipitation from pancreatic islets. We studied the antigenicity of two different radiolabelled preparations of GAD, d...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1996.d01-827.x

    authors： Rowley MJ,Chen QY,Teoh KL,Zimmet PZ,Tuomi T,Knowles WJ,Mackay IR

    更新日期：1996-11-01 00:00:00

  • Membrane phenotype and functional behaviour of T lymphocytes in multiple myeloma: correlation with clinical stages of the disease.

    abstract：:The distribution of T lymphocyte subsets was assessed using monoclonal antibodies (MoAbs) in 44 untreated patients with multiple myeloma (MM) subdivided according to the clinical stage of the disease. A significant reduction (P less than 0.001) of T lymphocytes was observed only in stage II and III patients. The propo...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Lauria F,Foa R,Cavo M,Gobbi M,Raspadori D,Giubellino MC,Tazzari PL,Tura S

    更新日期：1984-06-01 00:00:00

  • Anti-thyroid drugs and lymphocyte function. I. The in vitro effect on blastogenesis and suppressor cell activity.

    abstract：:The in vitro effect of the anti-thyroid drugs (ATD), propylthiouracil (PTU) and methimazole (MMI) on blastogenesis of peripheral blood mononuclear cells (PBMC) from healthy subjects was studied in 72 hr PHA stimulated cultures. PTU in therapeutic concentrations (10 micrograms/ml) suppressed blastogenesis only when add...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Goldrath N,Eisenstein Z,Bank H,Shoham J

    更新日期：1982-10-01 00:00:00