当前位置： SCI文献检索 > CLINICAL AND EXPERIMENTAL IMMUNOLOGY期刊下所有文献 > Epitope analysis of insulin autoantibodies using recombinant Fab.

Epitope analysis of insulin autoantibodies using recombinant Fab.

Abstract：

:Autoantibodies to insulin are often the first autoantibodies detected in young children with type 1 diabetes and can be present before the onset of clinical diabetes. These autoantibodies and their epitopes are, however, not well characterized. We explored the use of monoclonal antibodies and their recombinant Fab as reagents for epitope analysis. In this study we cloned and characterized the recombinant Fab of the insulin-specific monoclonal antibody CG7C7. We found the epitope of this antibody to be located predominantly at the A-chain loop of the insulin molecule. The recombinant Fab was then used to compete for insulin binding against insulin autoantibodies present in sera from patients with type 1 or type 1.5 diabetes. In competition experiments with sera positive for autoantibodies to insulin the recombinant Fab significantly reduced the binding to [125I]-insulin by sera of type 1 (n = 35) and type 1.5 diabetes [latent autoimmune diabetes in adults (LADA)] (n = 14) patients (P < 0.0001). We conclude that competition between insulin-specific monoclonal antibodies or their recombinant Fab and insulin autoantibodies should prove useful in the epitope analysis of autoantibodies to insulin.

journal_name

Clin Exp Immunol

journal_title

Clinical and experimental immunology

authors

Padoa CJ,Crowther NJ,Thomas JW,Hall TR,Bekris LM,Torn C,Landin-Olsson M,Ortqvist E,Palmer JP,Lernmark A,Hampe CS

doi

10.1111/j.1365-2249.2005.02802.x

subject

Has Abstract

pub_date

2005-06-01 00:00:00

pages

564-71

issue

3

eissn

0009-9104

issn

1365-2249

pii

CEI2802

journal_volume

140

pub_type

杂志文章

相关文献

CLINICAL AND EXPERIMENTAL IMMUNOLOGY文献大全
  • Immune response to rabbit liver-specific lipoprotein in acute viral hepatitis.

    abstract：:A serial prospective study of cell-mediated immunity to rabbit liver-specific lipoprotein (RLSP) has been done in 26 patients with acute viral hepatitis (AH) (18 HBsAg+ and eight HBsAg-) using a lymphocyte transformation test. An increased stimulation index was recorded in 56% of HBsAg+ cases and in 63% of the HBsAg- ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Ortona L,Laghi V,Cauda R,Nervo P

    更新日期：1980-12-01 00:00:00

  • Studies of the molecular basis of IgA production, subclass regulation and class-switch recombination in IgA nephropathy patients.

    abstract：:IgA nephropathy (IgAN), the most common form of glomerulonephritis, is characterized by normal to elevated levels of serum IgA. In order to understand the molecular mechanism(s) involved in the production of IgA in IgAN, peripheral blood mononuclear cells (PBMC) from these patients were analysed in this study. IL-10, ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1996.d01-859.x

    authors： Baskin B,Pettersson E,Rekola S,Smith CI,Islam KB

    更新日期：1996-12-01 00:00:00

  • The interaction of anti-glomerular basement membrane antibody deposition with immune elimination of bovine serum albumin in the rabbit.

    abstract：:We studied the interaction of two different forms of immune glomerular damage occurring simultaneously: anti-glomerular basement membrane (GBM) antibody fixation and immune elimination of bovine serum albumin (BSA). 125I-radiolabelled BSA anti-BSA immune complexes, formed in response to a single small intravenous dose...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Trevillian P,Cameron JS

    更新日期：1979-03-01 00:00:00

  • Nickel, cobalt, chromium, palladium and gold induce a mixed Th1- and Th2-type cytokine response in vitro in subjects with contact allergy to the respective metals.

    abstract：:Nickel (Ni), the main cause of contact allergy to metals, induces in vitro production of both Th1- and Th2-type cytokines in peripheral blood mononuclear cells (PBMC) from allergic subjects. Because the knowledge of the cellular immune response to other metals involved in contact allergy has been limited, we investiga...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2006.03226.x

    authors： Minang JT,Areström I,Troye-Blomberg M,Lundeberg L,Ahlborg N

    更新日期：2006-12-01 00:00:00

  • Toll-like receptor (TLR) 4 polymorphisms are associated with a chronic course of sarcoidosis.

    abstract：:The aetiology of sarcoidosis, an inflammatory granulomatous multi-system disorder, is unclear. It is thought to be the product of an unknown exogenous antigenic stimulus and an endogenous genetic susceptibility. Toll-like receptors (TLR) are signal molecules essential for the cellular response to bacterial cell wall c...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2006.03008.x

    authors： Pabst S,Baumgarten G,Stremmel A,Lennarz M,Knüfermann P,Gillissen A,Vetter H,Grohé C

    更新日期：2006-03-01 00:00:00

  • Regulation of experimental autoimmune uveitis in rats--separation of MHC and non-MHC gene effects.

    abstract：:Experimental autoimmune uveitis (EAU) is an organ-specific autoimmune disease and has served as a model of certain ocular inflammatory conditions in man. The present study was aimed at separating the effects of MHC and non-MHC genes on the development of EAU in the rat. EAU-susceptible LEW (RT1l), EAU-resistant WKAH (...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1991.tb02947.x

    authors： Hirose S,Ogasawara K,Natori T,Sasamoto Y,Ohno S,Matsuda H,Onoé K

    更新日期：1991-12-01 00:00:00

  • Serum amyloid P component and autoimmune parameters in the assessment of arthritis activity in MRL/lpr/lpr mice.

    abstract：:The concentration of immunoglobulins, anti-ssDNA, anti-dsDNA, anti-TNP antibodies, IgM rheumatoid factor, C3, immune complexes and serum amyloid P component (SAP), in the serum were measured in 68 male and female MRL/lpr/lpr mice, a strain affected with a systemic autoimmune disease. The degree of lymphoproliferation ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Rordorf-Adam C,Serban D,Pataki A,Grüninger M

    更新日期：1985-09-01 00:00:00

  • Analysis of autoantibody production in SCID-systemic lupus erythematosus (SLE) chimeras.

    abstract：:Mice with SCID disease have previously been successfully engrafted with human peripheral blood mononuclear cells (PBMC) obtained from normal individuals and from patients with various diseases. To determine whether SCID mice engrafted with SLE PBMC produced autoantibodies with specificities similar to those in the SLE...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1992.tb03043.x

    authors： Ashany D,Hines J,Gharavi A,Mouradian J,Elkon KB

    更新日期：1992-04-01 00:00:00

  • Increased IgE-dependent cytotoxicity by blood mononuclear cells of allergic patients.

    abstract：:Peripheral blood mononuclear cells from 14 healthy donors and 22 allergic patients were incubated with 51Cr-labelled chicken erythrocytes coated with an IgE myeloma protein or rabbit IgG antibodies. Mononuclear cells from patients with severe atopic disorders released a significantly greater percentage of 51Cr (P less...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Melewicz FM,Zeiger RS,Mellon MH,O'Connor RD,Spiegelberg HL

    更新日期：1981-03-01 00:00:00

  • Immunosuppression with cyclosporin A alters the thymic microenvironment.

    abstract：:The effect of cyclosporin A (CyA) immunosuppression on the murine thymic microenvironment and T lymphocyte development has been analysed using monoclonal antibodies to epithelial and lymphocyte subpopulations, macrophages and major histocompatibility complex (MHC) class II antigens in immunohistochemistry and flow cyt...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Kanariou M,Huby R,Ladyman H,Colic M,Sivolapenko G,Lampert I,Ritter M

    更新日期：1989-11-01 00:00:00

  • Arthritogenic actions of recombinant IL-1 and tumour necrosis factor alpha in the rabbit: evidence for synergistic interactions between cytokines in vivo.

    abstract：:Intra-articular injection of highly purified or recombinant interleukin 1 (IL-1) into the rabbit knee induces a transient synovitis with leucocytic infiltration into the synovial lining and joint cavity and loss of proteoglycan from articular cartilage. Tumour necrosis factor alpha (TNF-alpha), which has many of the a...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Henderson B,Pettipher ER

    更新日期：1989-02-01 00:00:00

  • Ethyl pyruvate significantly inhibits tumour necrosis factor-α, interleukin-1β and high mobility group box 1 releasing and attenuates sodium taurocholate-induced severe acute pancreatitis associated with acute lung injury.

    abstract：:In this study, we examined the effect of ethyl pyruvate (EP) on pulmonary inflammation in rats with severe pancreatitis-associated acute lung injury (ALI). Severe acute pancreatitis (SAP) was induced in rats by the retrograde injection of 5% sodium taurocholate into the pancreatic duct. Rats were randomly divided into...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12062

    authors： Luan ZG,Zhang J,Yin XH,Ma XC,Guo RX

    更新日期：2013-06-01 00:00:00

  • Neopterin release from human endothelial cells is triggered by interferon-gamma.

    abstract：:Human umbilical vein endothelial cells (HUVEC) were investigated for their ability to produce neopterin, a biochemical marker for an activated immune system. Interferon-gamma (IFN-gamma), IL-1 alpha, IL-2, IL-6, tumour necrosis factor-alpha, granulocyte/macrophage colony stimulating factor, phytohaemagglutinin and con...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1992.tb06486.x

    authors： Andert SE,Griesmacher A,Zuckermann A,Müller MM

    更新日期：1992-06-01 00:00:00

  • Alteration of Fas and Fas ligand expression during human visceral leishmaniasis.

    abstract：:Several studies in murine systems have suggested a role of apoptosis in the pathogenesis of leishmaniasis. However, the role of apoptosis in visceral leishmaniasis in man has not been explored. In this study, we show that patients with visceral leishmaniasis demonstrate significant dysregulation of Fas and Fas ligand....

    journal_title：Clinical and experimental immunology

    pub_type： 临床试验,杂志文章,多中心研究

    doi：10.1046/j.1365-2249.2002.01976.x

    authors： Eidsmo L,Wolday D,Berhe N,Sabri F,Satti I,El Hassan AM,Sundar S,Chiodi F,Akuffo H

    更新日期：2002-11-01 00:00:00

  • Airway-specific recruitment of T cells is reduced in a CD26-deficient F344 rat substrain.

    abstract：:Asthma is a chronic inflammatory disease affecting the airways. Increased levels of T cells are found in the lungs after the induction of an allergic-like inflammation in rats, and flow cytometry studies have shown that these levels are reduced in CD26-deficient rats. However, the precise anatomical sites where these ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2009.03991.x

    authors： Schade J,Schmiedl A,Kehlen A,Veres TZ,Stephan M,Pabst R,von Hörsten S

    更新日期：2009-10-01 00:00:00

  • Identification of autoantigens in canine autoimmune haemolytic anaemia.

    abstract：:Autoantigens in canine autoimmune haemolytic anaemia (AIHA) were identified by immunoprecipitation using autoantibody eluted from the erythrocytes of affected dogs. At least three patterns of precipitated antigen were identified in six cases of AIHA. The most commonly precipitated antigen pattern was a combination of ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1991.tb05678.x

    authors： Barker RN,Gruffydd-Jones TJ,Stokes CR,Elson CJ

    更新日期：1991-07-01 00:00:00

  • Generation and functional analysis of T cell lines and clones specific for schistosomula released products (SRP-A).

    abstract：:Antigens present in the products released by the larval stage of schistosome (SRP-A) were shown to induce a strong cytotoxic and protective IgE response both in the rat and the monkey. T cell lines and clones specific for SRP-A or 26 kD antigens which are the main target of the cytotoxic IgE have been derived. The pas...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Damonneville M,Velge F,Verwaerde C,Pestel J,Auriault C,Capron A

    更新日期：1987-08-01 00:00:00

  • Oxidized low-density lipoprotein (Ox-LDL) but not LDL aggravates the manifestations of experimental antiphospholipid syndrome (APS).

    abstract：:Ox-LDL is thought to play a major role in atherogenesis. The mechanisms mediating the deleterious influences of Ox-LDL include foam cell formation and cell cytotoxicity. The production of anti-Ox-LDL antibodies results in the formation of immune complexes which are taken up at enhanced rate by macrophages, leading to ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1997.d01-1019.x

    authors： George J,Blank M,Hojnik M,Bar-Meir E,Koike T,Matsuura E,Lorber M,Aviram M,Shoenfeld Y

    更新日期：1997-05-01 00:00:00

  • Epigenetic alterations on C1-inhibitor expression may influence hereditary angioedema attack frequency and C4 levels: Comment on: Karagianni P, Goules AV, Tzioufas AG. Epigenetic alterations in Sjogren's syndrome patient saliva.

    abstract：:Epigenetic studies reveal how our genes (nature) are influenced by environment (nurture) leading to wide variability in clinical presentations, especially in autoimmune diseases. Patients with C1-inhibitor deficiency, even within the same family, have diverse clinical presentations that may reflect epigenetic control ...

    journal_title：Clinical and experimental immunology

    pub_type： 评论,杂志文章

    doi：10.1111/cei.13516

    authors： Khan S,Longhurst H

    更新日期：2020-11-01 00:00:00

  • Intravenous immunoglobulin (IVIG) preparations induce apoptosis in TNF-alpha-stimulated endothelial cells via a mitochondria-dependent pathway.

    abstract：:Endothelial cells (ECs) are a target in inflammation, and the death of EC is regulated by various factors. Although intravenous immunoglobulin (IVIG) preparations are known to be beneficial therapeutic agents for the treatment of autoimmune diseases and systemic inflammatory disorders, their mechanism of action have n...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2002.01769.x

    authors： Nakatani K,Takeshita S,Tsujimoto H,Sekine I

    更新日期：2002-03-01 00:00:00

  • Immunological investigation in adenine phosphoribosyl transferase (APRT) deficiency.

    abstract：:In a family with partial or total adenine phosphoribosyl transferase (APRT) deficiency, T and B cell function was evaluated by counting rosette-forming cells, by investigating lymphocyte transformation to phytohaemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), by assaying serum immunoglobulins an...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Stevens WJ,Peetermans ME,Van Acker KJ

    更新日期：1979-06-01 00:00:00

  • In vitro monitoring of cadaver kidney donor pretreatment by lymphocyte culture.

    abstract：:Fourteen cadaver kidney donors were pretreated with a rapid infusion of 5 g of cyclophosphamide and 5 g of methylprednisolone in an attempt to reduce graft immunogenicity by altering immunogenic "passenger" leucocytes. The effects of this drug infusion on peripheral lymphocytes were monitored by measuring the ability ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Jeffery JR,Guttmann RD,Charpentier B

    更新日期：1976-09-01 00:00:00

  • IL-5 expressed by CD4+ lymphocytes from Echinococcus multilocularis-infected patients.

    abstract：:IL-5 is a major factor inducing differentiation of B lymphocytes into immunoglobulin-producing cells as well as a main regulator of eosinophils. Recently, we have shown that peripheral blood mononuclear cells (PBMC) from patients with alveolar echinococcosis (AE) express IL-5 mRNA after stimulation with crude Echinoco...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1997.4031299.x

    authors： Jenne L,Kilwinski J,Scheffold W,Kern P

    更新日期：1997-07-01 00:00:00

  • The amyloid in familial amyloid cardiomyopathy of Danish origin is related to pre-albumin.

    abstract：:Amyloid obtained from the myocardium of a patient (Han) with familial amyloid cardiomyopathy of Danish origin was studied. Gel filtration and electrophoresis of purified and denatured amyloid fibrils Han revealed various fractions ranging in mol. wt from 40,000 to 8,000 daltons. Amyloid Han and fractions reacted with ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Husby G,Ranløv PJ,Sletten K,Marhaug G

    更新日期：1985-04-01 00:00:00

  • The impact of an early truncating founder ATM mutation on immunoglobulins, specific antibodies and lymphocyte populations in ataxia-telangiectasia patients and their parents.

    abstract：:Eleven Norwegian patients (aged 2-33 years, seven males and four females) with Ataxia-telangiectasia (A-T) and their parents were investigated. Five of the patients were homozygous for the same ATM mutation, 3245delATCinsTGAT, a Norwegian founder mutation. They had the lowest IgG2 levels; mean (95% confidence interval...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2004.02492.x

    authors： Stray-Pedersen A,Jónsson T,Heiberg A,Lindman CR,Widing E,Aaberge IS,Borresen-Dale AL,Abrahamsen TG

    更新日期：2004-07-01 00:00:00

  • Desialylation of dying cells with catalytically active antibodies possessing sialidase activity facilitate their clearance by human macrophages.

    abstract：:Recently we reported the first known incidence of antibodies possessing catalytic sialidase activity (sialidase abzymes) in the serum of patients with multiple myeloma and systemic lupus erythematosus (SLE). These antibodies desialylate biomolecules, such as glycoproteins, gangliosides and red blood cells. Desialylati...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12312

    authors： Tomin A,Dumych T,Tolstyak Y,Kril I,Mahorivska I,Bila E,Stoika R,Herrmann M,Kit Y,Bilyy R

    更新日期：2015-01-01 00:00:00

  • Monocyte IgG-Fc receptors in myotonic dystrophy.

    abstract：:Myotonic dystrophy (MyD), an autosomal dominant neuromuscular disease with multisystem abnormalities, is associated with hypercatabolism of IgG. The hypercatabolism is not related to structural abnormalities of the IgG molecule in MyD but appears to be due to a derangement of the serum IgG concentration-fractional cat...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Banerjee D,McClintock J,Silver MM,Hudson AJ

    更新日期：1982-12-01 00:00:00

  • Inhibition of immune reactions in vivo by liposome associated transforming growth factor (TGF) type beta 1.

    abstract：:In view of its potent inhibitory capacity on immune cells in culture, we wished to determine the ability of transforming growth factor (TGF) beta 1 to down-regulate immune responses in vivo. Preliminary experiments suggested that, at the doses used, systemic injection of soluble TGF beta 1 could not affect bacterial-i...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1991.tb02965.x

    authors： Strassmann G,Bertolini DR,Eidelman O

    更新日期：1991-12-01 00:00:00

  • Immunity to herpes simplex virus in children receiving treatment for acute lymphoblastic leukaemia (ALL).

    abstract：:Children receiving anti-leukaemic therapy often become susceptible to severe herpesvirus infection. To investigate specific immunity to herpes simplex virus (HSV) in patients with acute lymphoblastic leukaemia (ALL), in first remission and receiving maintenance chemotherapy, we measured their HSV specific T responder ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Tellez PA,Odom L,Hayward AR

    更新日期：1985-12-01 00:00:00

  • Fractionation of mycobacterial integral membrane proteins by continuous elution SDS-PAGE reveals the immunodominance of low molecular weight subunits for human T cells.

    abstract：:Integral membrane proteins (IMP) represent a serologically distinct class of mycobacterial antigens which are potent stimulators of human T cells (Mehrotra et al., Clin Exp Immunol 1995; 102:626). The range of IMP from Mycobacterium fortuitum was resolved by continuous elution SDS-PAGE to recover 31 discrete fractions...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1997.4531351.x

    authors： Mehrotra J,Mittal A,Dhindsa MS,Sinha S

    更新日期：1997-09-01 00:00:00