Abstract：
:Several studies in murine systems have suggested a role of apoptosis in the pathogenesis of leishmaniasis. However, the role of apoptosis in visceral leishmaniasis in man has not been explored. In this study, we show that patients with visceral leishmaniasis demonstrate significant dysregulation of Fas and Fas ligand. Levels of soluble Fas (sFas) and soluble Fas ligand (sFasL) were elevated in plasma of patients with active visceral leishmaniasis (VL) and individuals co-infected with VL-HIV-1 compared to healthy controls. The levels of sFas and sFasL were normalized 6 months after successful treatment. In VL patients, the expression of membrane bound Fas, and to a lower extent FasL, were up-regulated on Leishmania donovani-infected spleen cells, the site of parasite multiplication. Expression of Fas and FasL on peripheral blood mononuclear cells was within normal range, probably reflecting that the blood is not a normal site of L. donovani infection. Furthermore, this is suggested by the finding that in vitro infection of macrophages with L. donovani up-regulated Fas expression on the surface of infected cells and enhanced the levels of sFasL in supernatants from infected cultures. How this dysregulation may affect the pathogenesis of human visceral leishmaniasis is discussed.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Eidsmo L,Wolday D,Berhe N,Sabri F,Satti I,El Hassan AM,Sundar S,Chiodi F,Akuffo Hdoi
10.1046/j.1365-2249.2002.01976.xsubject
Has Abstractpub_date
2002-11-01 00:00:00pages
307-13issue
2eissn
0009-9104issn
1365-2249pii
1976journal_volume
130pub_type
临床试验,杂志文章,多中心研究abstract：:Spleens of BALB/c mice with plasmacytoma contain macrophages (M phi) which were found to suppress the in vitro immune function of normal splenic cells through a diffusible factor. The suppressor activity of these macrophages was reversibly inhibited by ligands specific for the corresponding PC globulin, but not by ant...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-11-01 00:00:00
abstract：:The cytotoxic functions of highly purified blood lymphocytes from patients with breast cancer were studied before and after radiotherapy. Addition of PHA or of rabbit antibodies to target cells (chicken erythrocytes) were chosen as two means of inducing lymphocyte cytotoxicity in vitro. The proportion of T and non-T-l...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1975-11-01 00:00:00
abstract：:Whilst the mechanism by which Helicobacter pylori causes different gastroduodenal diseases is uncertain, strains producing the cytotoxin-associated protein (CagA) have greater pathogenicity. Hsps are immunogenic molecules induced by inflammatory mediators. The aim of this study was to assess pathogenicity of hsp antib...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1998.00608.x
更新日期：1998-06-01 00:00:00
abstract：:The C77G polymorphism in exon A of the human CD45 gene occurs with low frequency in healthy individuals. An enhanced frequency of C77G individuals has been reported in cohorts of patients suffering from multiple sclerosis, systemic sclerosis, autoimmune hepatitis, hepatitis C and human immunodeficiency virus (HIV)-1. ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2007.03508.x
更新日期：2007-12-01 00:00:00
abstract：:Ws/Ws rats have a small deletion of the c-kit gene, and are deficient in both mucosal and connective tissue-type mast cells. In this study, the role of mucosal type mast cells (MMC) in the development of intestinal ischaemia-reperfusion injury was investigated in Ws/Ws rats. Autoperfused segments of the jejunum were e...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1999.00851.x
更新日期：1999-04-01 00:00:00
abstract：:HAART may increase CD4+ T cell counts despite a persistently detectable HIV load. The impact of HAART on apoptosis, which may play a role in the disease process in HIV-infected patients, has not been extensively studied. We performed a study to compare the level of spontaneous T cell apoptosis and anti-retroviral trea...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1999.01076.x
更新日期：1999-12-01 00:00:00
abstract：:Recently we described the occurrence of B cells producing polyspecific natural IgM with anti-tumour specificity in the spleen of non-tumour-bearing individuals as well as in fetal organisms. Immunoprecipitation and 2-D electrophoresis showed the binding of such antibodies to a 55-kD (pI 6.0) membrane surface glycoprot...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1995.tb05561.x
更新日期：1995-03-01 00:00:00
abstract：:Diagnosis of perinatal infection in the newborn is difficult; there may be few clinical signs and current tests are slow or non-specific. Detection of organisms, antigen or specific antibody to common pathogens often requires repeat samples and does not give immediate results. Haematological parameters, although relie...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1998.00626.x
更新日期：1998-07-01 00:00:00
abstract：:The number of NK T cells was measured in relation to the Th1/Th2 imbalance observed in RA. Peripheral blood samples of patients with RA (n = 60) and healthy controls (n = 36) were stained with anti-NK receptor 1A (anti-NKR-P1A), anti-CD56, and anti-CD3 MoAbs, and examined by three-colour flow cytometry. NK T (NKR-P1A+...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1999.01018.x
更新日期：1999-10-01 00:00:00
abstract：:Amyloid obtained from the myocardium of a patient (Han) with familial amyloid cardiomyopathy of Danish origin was studied. Gel filtration and electrophoresis of purified and denatured amyloid fibrils Han revealed various fractions ranging in mol. wt from 40,000 to 8,000 daltons. Amyloid Han and fractions reacted with ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:Complement receptor 1-related gene/protein y (Crry) is a potent murine complement regulator that inhibits C3 convertases. Transgenic mice that overexpress soluble Crry (sCrry), directed systemically by the metallothionein-I promoter, have been used as an animal model for chronic blockade of complement activation. Rece...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2004.02450.x
更新日期：2004-05-01 00:00:00
abstract：:Anti-CD2 MoAbs have previously been shown to induce tolerance and to block B cell differentiation, T cell and monocyte activation. Since these immune functions are important in joint inflammation, we asked whether administration of the anti-CD2 MoAb OX34 has a beneficial effect on established rat adjuvant arthritis, a...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1997.4881385.x
更新日期：1997-10-01 00:00:00
abstract：:In this paper, we report the presence on Epstein-Barr virus-transformed lymphoblastoid cell lines on platelets and on fibroblasts of an HLA-B27-associated cell surface complex (antigenically related to some antigens of Klebsiella K43 and K21) which is identical to or cross-reactive with the determinant present on the ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-07-01 00:00:00
abstract：:A monoclonal IgM smooth muscle antibody secreted by a hybrid (MMI-1) of mouse plasmacytoma NS-1 with spleen cells from mouse immunized with Treponema pallidum was detected by indirect immunofluorescence tests on frozen tissue sections and on acetone fixed monolayers of rat and human fibroblasts. The antibody did not r...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1983-06-01 00:00:00
abstract：:Anti-cardiolipin antibodies, oxidatively modified low-density lipoproteins (oxLDL) and circulating immune complexes are humoral factors that have been linked to vascular damage. To analyse their possible role in the vascular complications in type 1 diabetes mellitus, we investigated patients with and without vascular ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1999.00788.x
更新日期：1999-02-01 00:00:00
abstract：:Autoimmune T cell responses directed against insulin-producing β cells are central to the pathogenesis of type 1 diabetes (T1D). Detection of such responses is therefore critical to provide novel biomarkers for T1D 'immune staging' and to understand the mechanisms underlying the disease. While different T cell assays ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2249.2010.04272.x
更新日期：2011-01-01 00:00:00
abstract：:C4BP has a central role in regulating the classical complement (C') pathway, but it is still uncertain whether or not it is consumed during in vivo complement activation. Attempts to demonstrate changes in C4BP plasma levels in systemic lupus erythematosus and essential mixed cryoglobulinaemia have failed, probably du...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1999.00874.x
更新日期：1999-05-01 00:00:00
abstract：:We have investigated TNF-alpha secretory response of peripheral blood mononuclear cells (PBMC) from 13 uraemic patients undergoing regular haemodialysis with cuprophane membrane (CM). Sixteen healthy subjects and five uraemic patients under conservative therapy were also studied as controls. Cells of haemodialysis pat...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1992.tb05830.x
更新日期：1992-10-01 00:00:00
abstract：:In general, exogenous proteins are processed by antigen-presenting cells in the endosomes for major histocompatibility complex (MHC) class II presentation to CD4+ T cells, while proteins synthesized endogenously are processed in the cytoplasm for MHC class I presentation to CD8+ T cells. However, it is recognized that...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2001.01613.x
更新日期：2001-09-01 00:00:00
abstract：:Autoimmune diseases are either tissue-specific like multiple sclerosis (MS) or multisystemic like systemic lupus erythematosus (SLE), although clinically both exhibit common features. To gain insight into the properties of the genes involved in each disease we have investigated the gene expression signature of periphe...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2004.02587.x
更新日期：2004-10-01 00:00:00
abstract：:The biological role of T cell receptor (TCR) gamma delta bearing cells is currently not fully understood. Recently, a monoclonal antibody (TCR delta 1) reacting against the whole molecule became available which facilitates the direct analysis of TCR-gamma delta+ cells. We studied 11 children with atopic dermatitis, 20...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1991.tb02950.x
更新日期：1991-12-01 00:00:00
abstract：:The high incidence of infections characteristic of impaired cell-mediated immunity in patients with hairy cell leukemia led us to study T lymphocyte function in sixteen patients with the lymphocyte transformation test. All patients showed imparied responses to mitogens, attributable to one or more of the following cau...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-11-01 00:00:00
abstract：:Recent advances in immunological and genetic research in coeliac disease provide new and complementary insights into the immune response driving this chronic intestinal inflammatory disorder. Both approaches confirm the central importance of T cell-mediated immune responses to disease pathogenesis and have further beg...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2249.2008.03704.x
更新日期：2008-08-01 00:00:00
abstract：:Previous studies show that endoplasmic reticulum-associated aminopeptidase (ERAP1/ERAP2) and runt-related transcription factor 3 (RUNX3) gene polymorphisms are associated with AS (ankylosing spondylitis) in European Caucasians. However, contradictory results were reported in different Asian populations. The purpose of...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13121
更新日期：2018-07-01 00:00:00
abstract：:The effect of low-density lipoprotein (LDL) on the binding of DNA and DNA-anti-DNA immune complexes to cultured human skin fibroblasts was examined. Using radioisotope analysis, ELISA and indirect immunofluorescent staining, a correlation between plasma membrane-bound DNA or DNA-anti-DNA immune complexes and cell-asso...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1991.tb05643.x
更新日期：1991-03-01 00:00:00
abstract：:F-42, an autoantibody found in the sera of some patients with systemic lupus erythematosus, was isolated from sera of seven different patients. By binding of the autoantibodies to cell bound C42, the autoantibodies were purified to homogeneity and radiolabelled with 125I. Purified preparations of 125I-F-42 were bound ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-06-01 00:00:00
abstract：:In order to study the role of gamma/delta T cells in the pathogenesis of inflammatory bowel disease (IBD) in humans, we measured the percentage of these cells in the peripheral blood, assessed the ratio of the non-disulphide-linked (delta TCS1) type of T cell receptor (TCR) in the total gamma/delta T cells, studied th...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1994.tb06611.x
更新日期：1994-10-01 00:00:00
abstract：:Cattle, infected with Fasciola hepatica metacercariae, produce antibodies against the outer glycocalyx of freshly excysted juvenile F. hepatica. Using 51Cr-release and viability assays such antibodies in the presence or absence of bovine complement did not cause discernible damage to the parasite. The presence of exce...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-09-01 00:00:00
abstract：:IgE was isolated from a patient with the hyper IgE, recurrent infection syndrome by immunoadsorption on sepharose bound goat anti-human IgE. Addition of this IgE to a monolayer culture of human monocytes resulted in a dose-dependent increase in PGE2 secretion. The addition of F(ab')2 fraction of goat anti-human IgE in...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
abstract：:During HIV-1 infection, the current tenet is that only anti-gag antibodies decline, while those directed at env remain stable. Among the latter antibodies, those directed to the V3 domain of gp120 are assumed to play a role in the immune surveillance against HIV-1. We investigated the correlation between anti-V3 antib...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1995.tb05567.x
更新日期：1995-03-01 00:00:00
