Abstract：
:In general, exogenous proteins are processed by antigen-presenting cells in the endosomes for major histocompatibility complex (MHC) class II presentation to CD4+ T cells, while proteins synthesized endogenously are processed in the cytoplasm for MHC class I presentation to CD8+ T cells. However, it is recognized that exogenous proteins can be processed for MHC class I presentation also, and evidence in favour of alternatives to the conventional MHC class I processing and presentation pathway is accumulating. Here, we show that exogenous recombinant influenza A virus nucleoprotein (rNP) is processed for MHC class I presentation to CD8+ cytotoxic T lymphocytes (CTL) by EBV-transformed, B-lymphoblastoid cell lines (B-LCL). Processing of rNP for HLA-B27-associated presentation seemed to follow the conventional MHC class I pathway predominantly, as presentation was diminished in the presence of lactacystin and brefeldin A, but was less sensitive to chloroquine and NH4Cl. HLA-B27-associated presentation was also observed using cells lacking a functional transporter associated with antigen processing, suggesting that alternative pathways may be exploited for processing of rNP.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Voeten JT,Rimmelzwaan GF,Nieuwkoop NJ,Fouchier RA,Osterhaus ADdoi
10.1046/j.1365-2249.2001.01613.xsubject
Has Abstractpub_date
2001-09-01 00:00:00pages
423-31issue
3eissn
0009-9104issn
1365-2249pii
cei1613journal_volume
125pub_type
杂志文章abstract：:Factor H and the FHL-1/reconectin protein are two human plasma proteins that act as important regulators of the alternative complement pathway. Each protein is encoded by a unique transcript, but both mRNAs are derived from the factor H gene by means of alternative processing. In order to address potential functional ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2000.01285.x
更新日期：2000-08-01 00:00:00
abstract：:Thymic biopsies taken under general anaesthetic prior to partial thyroidectomy for non-toxic nodular goitre were studied with histometric techniques: evidence is presented that the tissue studied is representative of the normal adult human thymus. The pattern of age involution of the normal adult thymus is described: ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1975-02-01 00:00:00
abstract：:The aim of the study was to test whether normal rat epiphyseal chondrocytes react with monoclonal antibodies (MoAbs) detecting different markers of lymphoid cells. For this purpose, isolated chondrocyte and, for comparison, splenocyte cytosmears were exposed to a battery of different MoAbs followed by indirect immunop...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:People receiving therapy with either 8-methoxypsoralen plus long wavelength ultraviolet radiation (PUVA) or medium wavelength ultraviolet radiation (UVB) have been studied to see whether their unresponsiveness to the contact sensitizer dinitrochlorobenzene (DNCB) is a form of immunological tolerance. It was confirmed ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:The production of anti-tetanus toxoid (TT) antibody and anti-anti-TT (auto-anti-Id) antibody has been measured in three young and three old persons. Both antibodies were measured using ELISA. We found that: (i) the anti-TT response of old subjects was lower than that of young subjects, (ii) serum auto-anti-Id antibody...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1993.tb05965.x
更新日期：1993-04-01 00:00:00
abstract：:IL-5 is a major factor inducing differentiation of B lymphocytes into immunoglobulin-producing cells as well as a main regulator of eosinophils. Recently, we have shown that peripheral blood mononuclear cells (PBMC) from patients with alveolar echinococcosis (AE) express IL-5 mRNA after stimulation with crude Echinoco...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1997.4031299.x
更新日期：1997-07-01 00:00:00
abstract：:T cells implicated in chronic inflammatory diseases such as RA respond weakly when stimulated in vitro with mitogen or antigen. The mechanism behind this hyporesponsiveness is unclear, but a depressed expression of the T cell receptor (TCR)-associated CD3zeta chain has been suggested. In the present work we describe a...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2000.01180.x
更新日期：2000-04-01 00:00:00
abstract：:Recent evidence has shown that microRNA-126 (miR-126) has been involved in the development and function of immune cells, which contributed to the pathogenesis of related clinical diseases. However, the potential role of miR-126 in the development and function of CD4+ T cells remains largely unknown. Here we first foun...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13067
更新日期：2018-02-01 00:00:00
abstract：:Regulatory T cells (T(regs)) may inhibit immunity against cancer. Induction and expansion of T(regs) in the immunosuppressive microenvironment created by a growing tumour appear to be one of the mechanisms by which it can evade host defence. We studied the impact of CD25+ T(regs) in a B cell lymphoma model in which Ra...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2008.03642.x
更新日期：2008-05-01 00:00:00
abstract：:Analysis of the VH gene repertoire of the J558 family was done in lipopolysaccharide (LPS)-stimulated resting cells and in vivo activated cells derived from C57Bl/6-lpr mice (IghCb). Using a restriction fragment length polymorphism (RFLP) based on digestion with the restriction enzyme Pstl, the expression of the subfa...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1995.tb05543.x
更新日期：1995-02-01 00:00:00
abstract：:Anti-neutrophil cytoplasmic antibodies (ANCA) against proteinase 3 (PR3) are strongly associated with Wegener's granulomatosis (WG) and are thought to be involved in its pathogenesis. Levels of PR3-ANCA do not always correspond to clinical disease activity. To investigate the relationship between functional effects of...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2002.01926.x
更新日期：2002-09-01 00:00:00
abstract：:The HLA haplotype A 10,B18 has been associated with hereditary deficiency of the second component of complement(C2). In an effort to detect individuals homozygous for C2 deficiency, a thorough audit of HLA serotyping results in 3,100 individuals was performed, and a single patient homozygous for the A10, B18 haplotype...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-11-01 00:00:00
abstract：:Autoantibodies in the sera of patients with Goodpasture's syndrome showed a strong reaction in indirect immunofluorescence tests on unfixed, frozen sections of a mouse tumour (EHS sarcoma), previously shown to produce extracellular basement membrane. Anti-basement membrane antibodies from patients with bullous pemphig...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:Autoantibodies directed against cytoplasmic antigens of neutrophils (ANCA), especially those with specificity for proteinase 3 (PR-3) and myeloperoxidase, are valuable markers for differential diagnosis and monitoring of disease activity in Wegener's granulomatosis (WG) and other vasculitides. Till now, several concep...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1994.tb06110.x
更新日期：1994-09-01 00:00:00
abstract：:Serum activity of the adenosine deaminase (ADA) isozyme, ADA2, has been reported to be elevated during various disease states. Macrophages have been suggested as the cellular source of extracellular ADA activity because they are one of the only cell types in which intracellular ADA2 activity has been measured, but ext...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2004.02591.x
更新日期：2004-10-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：:IFN-gamma is known to be a major inhibitor of IgE synthesis in vitro. Recent studies demonstrating reduced production of IFN-gamma in children and adults with atopic dermatitis and elevated serum IgE suggest a similar role for this cytokine in vivo. The reasons for this reduced IFN-gamma production are not known. One ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1994.tb06016.x
更新日期：1994-01-01 00:00:00
abstract：:It has been shown that bronchoalveolar lavages (BALs) from patients with sarcoidosis and other interstitial lung diseases contain abnormally increased numbers of alveolar macrophages (AM) expressing antigens found on monocytes. The aim of this study was to compare the phenotype of AM from patients with sarcoidosis wit...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1991.tb05791.x
更新日期：1991-10-01 00:00:00
abstract：:Erythrocyte autoantibodies in NZB mice react by hemagglutination methods with exposed and hidden red cell antigens. The hidden antigens can be exposed by treatment with proteolytic enzymes. By indirect immunofluorescence one antibody population can be shown to react with modified red cells. In the present study the lo...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:Bone marrow transplantation (BMT) recipients are immunosuppressed and are at risk for contracting severe infections. Recently, adoptive transfer of immunity against hepatitis B virus (HBV) was documented in BMT recipients receiving bone marrow from 'naturally' HBV-infected individuals who recovered spontaneously, or t...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1994.tb06084.x
更新日期：1994-08-01 00:00:00
abstract：:Antigen-antibody complexes, composed of 125I-BSA and guinea-pig or rabbit antibody, were incubated at 37 degrees C with human blood cells suspended in autologous serum and kinetics of binding analysed. When purified polymorphonuclear (PMN) or mononuclear cells (MNC) were studied, maximum binding was observed within 8 ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-06-01 00:00:00
abstract：:Whether differences in foetoplacental weight and post-implantation mortality in rodents are secondary to heterosis and inbreeding depression or antigenic differences between mother and foetus has been a continuing controversy. To determine whether non-specific depression or stimulation of the maternal immune system af...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-12-01 00:00:00
abstract：:Transmembrane activator and calcium modulator and cyclophilin ligand interactor-immunoglobulin (TACI-Ig) is a human fusion protein that binds and neutralizes both B lymphocyte stimulator (BLyS), a cytokine shown to be a key regulator of B cell maturation, proliferation and survival, and a proliferation-inducing ligand...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2010.04293.x
更新日期：2011-02-01 00:00:00
abstract：:Antilactoferrin antibodies have been reported in patients with several autoimmune disorders, including primary biliary cirrhosis, autoimmune hepatitis and autoimmune cholangitis. We investigated the prevalence and the clinical significance of such autoreactivity in patients with autoimmune and viral chronic liver dise...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2001.01524.x
更新日期：2001-06-01 00:00:00
abstract：:Peripheral blood mononuclear cells from thirty-one patients with inflammatory bowel disease (ulcerative colitis and Crohn's disease) were analysed for the proportions and absolute numbers of total T cells, and for the T cell subpopulations carrying Fc receptors for either IgM (Tmu cells) or IgG (T gamma cells). Twenty...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-07-01 00:00:00
abstract：:Cytokines have been extensively studied in coeliac disease, but cytokine release related to exposure to gluten and associated symptoms has only recently been described. Prominent, early elevations in serum interleukin (IL)-2 after gluten support a central role for T cell activation in the clinical reactions to gluten ...
journal_title：Clinical and experimental immunology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1111/cei.13369
更新日期：2020-01-01 00:00:00
abstract：:We examined the effect of IL-6 on the development of autoimmune diseases (primary biliary cirrhosis, Sjögren's syndrome) employing murine graft-versus-host reaction (GVHR) model with MHC class II disparity. For this purpose, we used IL-6 transgenic (B6.6) mice in which a high level of IL-6 was detected. C57Bl/6 (B6) s...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1994.tb07030.x
更新日期：1994-03-01 00:00:00
abstract：:Neutrophil phagocytosis and killing of Candida albicans were examined using a radiometric assay in 25 patients with insulin-dependent diabetes and 17 controls under various in vitro metabolic conditions. Glucose was present at 5, 10 and 50 mM, beta-hydroxybutyrate at 1, 5 and 20 mM and glucose with beta-hydroxybutyrat...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-02-01 00:00:00
abstract：:CD5 is an antigen normally found on T cells and on a minority subpopulation of B cells in fetal spleen and tonsil and on the majority of cells in B-chronic lymphocytic leukaemia. Recent reports described the occurrence of large numbers of CD5-positive B cells in the peripheral blood after bone marrow transplantation (...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：:The introduction of 25 mg of a new protein antigen into the diets of mice produced transient increases in PVP clearance which reached a maximum 60 hr after the change of diet and had disappeared by 4 days. At the same time the immune responsiveness to unrelated antigens given parenterally was also raised transiently, ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-02-01 00:00:00
