Abstract:
:The aim of the study was to test whether normal rat epiphyseal chondrocytes react with monoclonal antibodies (MoAbs) detecting different markers of lymphoid cells. For this purpose, isolated chondrocyte and, for comparison, splenocyte cytosmears were exposed to a battery of different MoAbs followed by indirect immunoperoxidase staining. As we were able to show, all chondrocytes reacted with OX17 MoAb detecting Class II (Ia) antigen encoded by the RT1.D subregion of rat major histocompatibility complex (MHC). However, unlike splenocytes they did not react with OX6 MoAb detecting the RT1.B encoded Class II molecule. Moreover, all chondrocytes were stained with W3/25 MoAb specific for the rat equivalent of human CD4 (T4) antigen and with W3/13 MoAb specific for rat leucocyte sialoglycoprotein. A positive reaction was also obtained with the antibody against the S-100 protein. By contrast, chondrocytes did not react with antibodies specific for all T (OX19) or B (HIS14) cells, rat CD8 (T8) equivalent, monocytes/macrophages (ED1, ED2), factor VIII (M616), or glial fibrillary acidic protein (Z334).
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Malejczyk J,Romaniuk Asubject
Has Abstractpub_date
1989-03-01 00:00:00pages
477-80issue
3eissn
0009-9104issn
1365-2249journal_volume
75pub_type
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
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journal_title:Clinical and experimental immunology
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