Abstract：
:The production of interleukin-2 (IL-2) by Con A-activated spleen cells (SC) progressively declined and reached negligible values during the course of infection of C57BL/6 mice with Mycobacterium lepraemurium. In addition, the capacity of cultured SC to utilize IL-2 was highly reduced, as demonstrated by the accumulation of IL-2 activity in culture supernatants at 48 and 72 h after Con A activation. The depressed IL-2 utilization started to be observed about 1 to 2 weeks prior to the onset of the depressed IL-2 production and was not reversed by the addition of exogenous IL-2; thus implying that a lack of IL-2 utilization rather than a lack of IL-2 production could be directly responsible for the inhibition of T-cell proliferative responses to Con A in SC cultures of infected mice. The utilization of IL-2 was found to be down-regulated, at least in part, by splenic suppressor cells since, in mixed-culture experiments, SC from infected mice actively depressed the capacity of normal splenocytes to consume IL-2. Finally, the depressed IL-2 utilization would result from a 2- to 3-fold reduction of either or both the density of high-affinity IL-2 receptors and their affinity for IL-2.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Turcotte R,Lemieux Ssubject
Has Abstractpub_date
1989-04-01 00:00:00pages
126-31issue
1eissn
0009-9104issn
1365-2249journal_volume
76pub_type
杂志文章abstract：:The aim of this study was to determine the contribution of neutrophils to adjuvant arthritis (AA) by in vivo depletion of peripheral blood neutrophils. Specific anti-neutrophil MoAb, RP3 (10 mg), or a control antibody was given twice daily on days 8-11 after injection of Mycobacterium tuberculosis in inbred male Sprag...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1997.263-ce1154.x
更新日期：1997-02-01 00:00:00
abstract：:Lymphocytes isolated from Graves' and Hashimoto thyroid tissue by enzymatic (dispase) digestion or mechanical disaggregation were markedly different in terms of their ability to synthesize thyroid autoantibodies in culture. Dispase digestion, followed by removal of thyroid follicular cells, gave a lymphocyte populatio...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-08-01 00:00:00
abstract：:The Fc receptor mediated antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis induced by bispecific antibody (BsAb) to the high-affinity Fc receptor for IgG (Fc gamma RI) and to human red blood group antigen RhD were studied in vitro, using human mononuclear leucocytes as effector cells. The results w...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1992.tb06951.x
更新日期：1992-08-01 00:00:00
abstract：:We describe the usefulness of a fast (48-h) limiting dilution assay (LDA) for the enumeration of human alloreactive helper T lymphocytes (HTL) in the peripheral blood, in relation to histologically defined rejection grades after heart transplantation. HTL frequencies (HTLf) in pretransplant samples varied from patient...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1999.01091.x
更新日期：1999-12-01 00:00:00
abstract：:The antigen specificity of anti-phospholipid antibodies in infectious mononucleosis (IM) was studied using ELISA for the detection of anti-beta2-glycoprotein I (beta2-GPI), anti-annexin V, anti-protein S and anti-prothrombin antibodies and TLC immunostaining for the detection of anti-phospholipid antibodies. This tech...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2000.01206.x
更新日期：2000-05-01 00:00:00
abstract：:By using OKT monoclonal antibodies; OKT3(pan T), OKT4(inducer/helper), OKT8 (suppressor/cytotoxic) and OKIa1, T lymphocyte subsets were examined in lymphocytes of patients with tuberculosis both before and after in vitro stimulation with purified protein derivative of tuberculin (PPD). In freshly obtained lymphocytes ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
abstract：:In pulmonary sarcoidosis, the typical T helper 1-mediated immune response in the lungs has been proposed to be co-ordinated by regulatory T cells; however, their exact role needs to be clarified. We used real-time polymerase chain reaction to study genes involved in regulatory T cell functions in CD4+ T cells isolated...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2008.03609.x
更新日期：2008-04-01 00:00:00
abstract：:In order to establish normal reference ranges of serum immunoglobulin G subclasses for different age groups in Chinese, we measured the four IgG subclasses by radial immunodiffusion using polyclonal antisera in 350 normal healthy subjects (148 males and 202 females) recruited from the community. There was no significa...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1993.tb05905.x
更新日期：1993-02-01 00:00:00
abstract：:A ligand radioassay for the detection of IC which utilizes C1q, bovine conglutinin and low affinity rabbit IgM anti-human Fc in a reagent coctail, is presented. IC are first isolated from serum by precipitation in polyethylene glycol, then analysed for their ability to react with the ligand coctail. Dual-label studies...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:The specific response of human peripheral blood lymphocyte cultures to TNP-polyacrylamide was suppressed by the addition of concanavalin A (Con A). A dose of Con A (0.5 microgram/ml) could be selected, which induced a reproducible but incomplete suppression independent of the magnitude of the anti-TNP response. Con A-...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
abstract：:A complete functional deficiency of C1q is described in a patient suffering from SLE. From reduced plasma C1 activity of the parents a hereditary trait was assumed. The defective C1q molecule was haemolytically inactive, did not bind to immune complexes, and was not recognized by the monocyte C1q receptor. C1 activity...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1993.tb03442.x
更新日期：1993-11-01 00:00:00
abstract：:Sjögren's syndrome (SS) is an autoimmune disease characterized by clonal B cell attack of the exocrine glands and dysregulated expression of B cell-activating factor (BAFF). Based upon the current data of increased rates of lymphoid malignancy, as non-Hodgkin's lymphoma (NHL) is associated with SS, we propose the dete...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2010.04144.x
更新日期：2010-07-01 00:00:00
abstract：:Although the inhibitory effect of iron on macrophage production of tumoricidal free radical nitric oxide (NO) has been reported, its possible influence on macrophage anti-tumour activity has not been established. In the present study, FeSO4 markedly reduced IFN-gamma + LPS-induced NO synthesis in mouse and rat macroph...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2004.02515.x
更新日期：2004-07-01 00:00:00
abstract：:The chemotactic responsiveness of peripheral blood monocytes was evaluated in three groups of subjects: 32 patients with multiple myeloma, 27 subjects with benign monoclonal gammapathy, and 64 normal controls. Monocyte chemotactic responsiveness was significantly depressed both in multiple myeloma patients (P less tha...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-02-01 00:00:00
abstract：:Disseminated infection with virulent Mycobacterium avium in C57Bl/6 (B6) mice leads to severe lymphocyte depletion in secondary lymphoid organs. In this study, we found an up-regulation of caspase-8 activity in spleen cell extracts from M. avium 25291-infected B6 mice compared to non-infected mice. The activation of t...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2011.04385.x
更新日期：2011-06-01 00:00:00
abstract：:Quantification of human peripheral blood NK subsets has been made in a group of Kenyan adults and children with acute P. falciparum malaria. Results were compared with data obtained from three age- and sex-matched control cohorts: parasitaemic but asymptomatic children; aparasitaemic children and adults; and adult Cau...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1991.tb05768.x
更新日期：1991-10-01 00:00:00
abstract：:The structural characteristics of autologous red blood cell (ARBC) receptors on human peripheral blood lymphocytes (PBL) induced by phytohaemagglutinin (PHA) were examined. Tunicamycin, which is known to be a blocker of the protein glycosylation of N-glycosidic type glycoprotein, significantly inhibited the production...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
abstract：:Mixed cryoglobulinaemia (MC) is a disorder characterized by the presence of large amounts of cryoprecipitating IgM-IgG complexes. An immune complex glomerulonephritis develops in one third of all patients, but its occurrence does not seem related to the amount of cryoglobulins in the sera, nor to their complement-fixi...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1994.tb06560.x
更新日期：1994-05-01 00:00:00
abstract：:Antibody titres to whole ovary, theca cells, granulosa cells and endometrium were determined by passive haemagglutination and immunofluorescence assays in sera and in cervical and vaginal secretions from 13 patients with endometriosis. Antibody titres to endometrium (mean log2 +/- s.e.m., 7.08 +/- 0.80; P less than 0....
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-11-01 00:00:00
abstract：:The cytotoxic functions of highly purified blood lymphocytes from patients with breast cancer were studied before and after radiotherapy. Addition of PHA or of rabbit antibodies to target cells (chicken erythrocytes) were chosen as two means of inducing lymphocyte cytotoxicity in vitro. The proportion of T and non-T-l...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1975-11-01 00:00:00
abstract：:Organ-specific surface-reactive antibodies to viable human adrenal cell suspensions from adult or fetal glands were detected by indirect immunofluorescence (IFL) in 24 out of 28 idiopathic Addison's disease sera with adrenal cytoplasmic antibodies. Cell-surface reactions were also present in nine out of 10 cases of po...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-07-01 00:00:00
abstract：:Human red cells (RBC) coated with IgG anti-D are cleared from the circulation to the spleen by macrophages which express IgG receptors (Fcgamma R). Polymorphisms of Fcgamma RIIa and Fcgamma RIIIa affect IgG binding in vitro, and may alter the efficiency of clearance of immune complexes in vivo. In a RBC clearance stud...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2003.02119.x
更新日期：2003-04-01 00:00:00
abstract：:Previous studies have shown that male mice of strains with high target organ responsiveness to androgen, the C57L/J and RF/J, have lower plasma immunoglobulin levels and weaker antibody responses to protein and polysaccharide antigens than mice of low androgen responder (LAR) strains, the A/J and 129/J. In the current...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：:Maintenance of lung homeostasis involves a complex interaction between T lymphocytes and alveolar macrophages (AM), in which AM suppress pulmonary T cell proliferation to antigenic stimuli. To assess whether AM-mediated suppression is attenuated in healthy young infants, AM and peripheral blood mononuclear cells (PBMC...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2002.01848.x
更新日期：2002-05-01 00:00:00
abstract：:The lymphotoxin-beta receptor (LTbetaR) pathway is critical for maintenance of organized lymphoid structures and is involved in the development of colitis. To investigate the mechanisms by which LTbetaR activation contributes to the pathology of chronic inflammation we used a soluble LTbetaR-Ig fusion protein as a com...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2004.02402.x
更新日期：2004-04-01 00:00:00
abstract：:The requirement for specificity of antibody-dependent inhibition or killing of intracellular Toxoplasma gondii trophozoites by normal mouse peritoneal macrophages was evaluated in vitro using light microscopy and autoradiography. Anti-toxoplasma antibody in the presence of 'accessory factor' rendered extracellular T. ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-12-01 00:00:00
abstract：:Toll-like receptor 4 (TLR4), which recognizes lipopolysaccharides, plays an important role in the innate immune response. In this study, we investigated the role of TLR4 in the development of experimental colitis with regard to the biological actions of macrophage migration inhibitory factor (MIF) using TLR4 null ((-/...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2005.02877.x
更新日期：2005-09-01 00:00:00
abstract：:Oxazolone-induced colitis in mice has become a recognized model to study the efficacy of therapeutics targeting the immunological response underlying the development of inflammatory bowel disease. However, this model cannot be used when therapeutics designed to address human targets do not interact with the respective...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12057
更新日期：2013-05-01 00:00:00
abstract：:Circulating immune complexes were repeatedly searched for using the C1q-binding assay and the Raji cell test in Lewis and BN rats immunized with the Fx1A fraction from convoluted proximal tubule or with kidney homogenate, mixed with various adjuvants. An autoimmune glomerulonephritis was easily induced in Lewis rats. ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-10-01 00:00:00
abstract：:Statins reduce plasma cholesterol, but clinical trials and in vitro studies indicate that they might also possess anti-inflammatory properties. The effect of simvastatin on circulating cytokines and leucocytes was evaluated in a human in vivo model of low-grade inflammation. Thirty young healthy male participants rece...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,随机对照试验
doi：10.1111/j.1365-2249.2006.03042.x
更新日期：2006-04-01 00:00:00
