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Antibody to severe acute respiratory syndrome (SARS)-associated coronavirus spike protein domain 2 cross-reacts with lung epithelial cells and causes cytotoxicity.

Abstract：

:Both viral effect and immune-mediated mechanism are involved in the pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. In this study, we showed that in SARS patient sera there were autoantibodies (autoAbs) that reacted with A549 cells, the type-2 pneumocytes, and that these autoAbs were mainly IgG. The autoAbs were detectable 20 days after fever onset. Tests of non-SARS-pneumonia patients did not show the same autoAb production as in SARS patients. After sera IgG bound to A549 cells, cytotoxicity was induced. Cell cytotoxicity and the anti-epithelial cell IgG level were positively correlated. Preabsorption and binding assays indicated the existence of cross-reactive epitopes on SARS-CoV spike protein domain 2 (S2). Furthermore, treatment of A549 cells with anti-S2 Abs and IFN-gamma resulted in an increase in the adherence of human peripheral blood mononuclear cells to these epithelial cells. Taken together, we have demonstrated that the anti-S2 Abs in SARS patient sera cause cytotoxic injury as well as enhance immune cell adhesion to epithelial cells. The onset of autoimmune responses in SARS-CoV infection may be implicated in SARS pathogenesis.

journal_name

Clin Exp Immunol

journal_title

Clinical and experimental immunology

authors

Lin YS,Lin CF,Fang YT,Kuo YM,Liao PC,Yeh TM,Hwa KY,Shieh CC,Yen JH,Wang HJ,Su IJ,Lei HY

doi

10.1111/j.1365-2249.2005.02864.x

subject

Has Abstract

pub_date

2005-09-01 00:00:00

pages

500-8

issue

3

eissn

0009-9104

issn

1365-2249

pii

CEI2864

journal_volume

141

pub_type

杂志文章

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