Abstract:
:Booster immunization of normal individuals with soluble tetanus toxoid (TT) produced significant levels of plasma anti-tetanus IgG and IgM detectable by an enzyme linked immunosorbent assay (ELISA). Similar changes in circulating anti-TT antibody were found in mice following primary immunization. There was. however, no correlation observed between circulating anti-TT antibody response and the proliferative response of peripheral blood mononuclear cells exposed to soluble TT in the immunized individuals. The capacity of the peripheral blood mononuclear cells to produce specific anti-TT antibody in vitro was evaluated using a new microculture antibody synthesis enzyme-linked assay (MASELA). It was observed that the production of specific antibody in vitro correlated with the circulating anti-TT antibody level and not with the proliferative response. Peripheral blood mononuclear cells from these recently secondary immunized individuals produced greater specific anti-TT antibody when cultured in TT coated plastic wells than did controls. The potential utility of this technique in assessing response to immunization and basic immune competence is discussed.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Ershler WB,Moore AL,Hacker MPsubject
Has Abstractpub_date
1982-09-01 00:00:00pages
552-8issue
3eissn
0009-9104issn
1365-2249journal_volume
49pub_type
杂志文章abstract::Monoclonal anti-dermatan sulphate antibodies were produced with hybridization after immunizing BALB/c mice with dermatan sulphate (DS). These antibodies were tested for cross-reactivity to double-stranded DNA (dsDNA) and heat-denatured DNA (ssDNA). Five clones had binding activity to DS, dsDNA and ssDNA in enzyme-link...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章,评审
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1985-09-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2009.04036.x
更新日期:2010-01-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.13574
更新日期:2021-01-12 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1978-11-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1980-02-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2002.01854.x
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1999.01018.x
更新日期:1999-10-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2002.01745.x
更新日期:2002-02-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1991.tb05626.x
更新日期:1991-02-01 00:00:00
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1987-08-01 00:00:00
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1976-04-01 00:00:00
abstract::The production of anti-tetanus toxoid (TT) antibody and anti-anti-TT (auto-anti-Id) antibody has been measured in three young and three old persons. Both antibodies were measured using ELISA. We found that: (i) the anti-TT response of old subjects was lower than that of young subjects, (ii) serum auto-anti-Id antibody...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1993.tb05965.x
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::Immune repopulation, despite virological failure, often occurs in children under highly active anti-retroviral therapy (HAART). The aim of this study was to analyse the characteristics of immune repopulation and activation in children with and without virological response to HAART. Fourteen human immunodeficiency viru...
journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1999.00874.x
更新日期:1999-05-01 00:00:00
abstract::The distribution and regulatory function of immunoglobulin (Ig) synthesis by monocytes were assessed using several monoclonal antibodies and a cell sorter in samples from 13 patients with multiple myeloma (MM) and nine patients with benign monoclonal gammopathy (BMG). Since all of these cells were separated directly w...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章,评审
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更新日期:2019-05-01 00:00:00
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journal_title:Clinical and experimental immunology
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更新日期:2009-03-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
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更新日期:2011-11-01 00:00:00