Antisera to human IgE can also recognize IgA and IgM molecules as produced by selected B lymphoblastoid cell lines.

Abstract:

:A series of polyclonal and monoclonal anti-human IgE sera reacted with a panel of 11 selected IgA and IgM producing B lymphoblastoid cell lines (BLCLs) in cytoplasmic fluorescence, ELISA and immunoprecipitation assays. The reactions were discordant between the BLCLs and the individual antisera. The panel of 11 BLCLs was selected from over 500 BLCLs by an ELISA spot assay using a polyclonal anti-human IgE serum. The BLCLs produced exclusively IgM or IgA, both with either kappa or lambda light chains as shown by immunoprecipitation/PAGE and mRNA analyses. The data indicate that IgE antisera may recognize IgE-like epitopes on the variable parts of some IgM or IgA molecules. These reactivities of IgE antisera may lead to false positive results of serum IgE determinations. Selected BLCLs are useful targets for exposing such reactivities in IgE antisera.

journal_name

Clin Exp Immunol

authors

Hendriks RW,Thompson A,Kraakman ME,Drayer AL,Schuurman RK

subject

Has Abstract

pub_date

1988-11-01 00:00:00

pages

305-10

issue

2

eissn

0009-9104

issn

1365-2249

journal_volume

74

pub_type

杂志文章
  • Treatment effect of CDKN1A on rheumatoid arthritis by mediating proliferation and invasion of fibroblast-like synoviocytes cells.

    abstract::The objective of the present study was to evaluate the role of CDKN1A in rheumatoid arthritis (RA). Related gene expression data screened from Gene Expression Omnibus (GEO) were processed with network analysis. Protein-protein interaction was analysed through string database. Quantitative reverse transcription-polymer...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/cei.13161

    authors: Gang X,Xu H,Si L,Zhu X,Yu T,Jiang Z,Wang Y

    更新日期:2018-11-01 00:00:00

  • Dichotomy of the human T cell response to Leishmania antigens. II. Absent or Th2-like response to gp63 and Th1-like response to lipophosphoglycan-associated protein in cells from cured visceral leishmaniasis patients.

    abstract::The T cell response to different Leishmania donovani antigens was investigated using peripheral blood mononuclear cells (PBMC) from Kenyans cured of visceral leishmaniasis and non-exposed Danes. Crude promastigote and amastigote antigens both induced proliferation and interferon-gamma (IFN-gamma) production in PBMC fr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.1994.tb06044.x

    authors: Kurtzhals JA,Hey AS,Jardim A,Kemp M,Schaefer KU,Odera EO,Christensen CB,Githure JI,Olafson RW,Theander TG

    更新日期:1994-06-01 00:00:00

  • Immune complex binding efficiency of erythrocyte complement receptor 1 (CR1).

    abstract::C3b-coated immune complexes adhere to the complement receptor 1 (CR1, CD35) on human erythrocytes. This multi-valent binding might be favoured by the known clustering of CR1 and by the multiple C3b-binding sites on each CR1. The size of the CR1 clusters correlates directly with the number of CR1/erythrocytes, and the ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.1991.tb08116.x

    authors: Madi N,Paccaud JP,Steiger G,Schifferli JA

    更新日期:1991-04-01 00:00:00

  • Anti-lymphocyte antibodies in lethal mouse malaria. II. Induction of an autoantibody specific suppressor T cell by non-lethal P. yoelii.

    abstract::The anti-lymphocyte autoantibody response to irradiated lethal Plasmodium berghei malaria parasites in normal mice was significantly reduced when recipients were pre-treated with splenic T cells from mice recovered from a non-lethal Plasmodium yoelii infection. Suppression was specific for the autoantibody and did not...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: de Souza JB,Playfair JH

    更新日期:1983-10-01 00:00:00

  • Effect of oestrogen on Mycobacterium avium complex pulmonary infection in mice.

    abstract::The purpose of the present study was to elucidate the role of oestrogen in the pathogenesis of Mycobacterium avium complex (MAC) pulmonary disease, which occurs most frequently in postmenopausal women. The study was carried out in a murine infectious model using ovariectomized DBA/2 female mice. Infection with MAC was...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2249.2001.01474.x

    authors: Tsuyuguchi K,Suzuki K,Matsumoto H,Tanaka E,Amitani R,Kuze F

    更新日期:2001-03-01 00:00:00

  • The interaction of anti-glomerular basement membrane antibody deposition with immune elimination of bovine serum albumin in the rabbit.

    abstract::We studied the interaction of two different forms of immune glomerular damage occurring simultaneously: anti-glomerular basement membrane (GBM) antibody fixation and immune elimination of bovine serum albumin (BSA). 125I-radiolabelled BSA anti-BSA immune complexes, formed in response to a single small intravenous dose...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Trevillian P,Cameron JS

    更新日期:1979-03-01 00:00:00

  • Anti-inflammatory activity of salmeterol: down-regulation of cytokine production.

    abstract::Elevation of intracellular cAMP levels has been shown previously to inhibit cytokine secretion by various cell types in vitro. Since salmeterol is a beta 2-agonist which activates adenylate cyclase, its ability to inhibit cytokine production was evaluated. Though salmeterol, and the related drug albuterol, did not inh...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.1995.tb05573.x

    authors: Sekut L,Champion BR,Page K,Menius JA Jr,Connolly KM

    更新日期:1995-03-01 00:00:00

  • Immunoregulation of the in vitro anti-HBs antibody synthesis in chronic HBsAg carriers and in recently boosted anti-hepatitis B vaccine recipients.

    abstract::We report a study on immunoregulation of in vitro antibody to hepatitis B surface antigen (anti-HBs) synthesis induced by pokeweed mitogen (PWM) from peripheral blood mononuclear cells (PBMC) in chronic hepatitis B surface antigen (HBsAg) carriers and in 'high responders', (anti-HBs RIA ratio greater than or equal to ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Barnaba V,Valesini G,Levrero M,Zaccari C,Van Dyke A,Falco M,Musca A,Balsano F

    更新日期:1985-05-01 00:00:00

  • Macrophage migration inhibitory factor deficiency protects pancreatic islets from cytokine-induced apoptosis in vitro.

    abstract::During pathogenesis of diabetes, pancreatic islets are exposed to high levels of cytokines and other inflammatory mediators that induce deterioration of insulin-producing beta cells. Macrophage migration inhibitory factor (MIF) plays a key role in the onset and development of several immunoinflammatory diseases and al...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.2012.04607.x

    authors: Stojanovic I,Saksida T,Nikolic I,Nicoletti F,Stosic-Grujicic S

    更新日期:2012-08-01 00:00:00

  • Autoantibodies recognizing citrullinated rat filaggrin in an ELISA using citrullinated and non-citrullinated recombinant proteins as antigens are highly diagnostic for rheumatoid arthritis.

    abstract::The objective of the study was to determine the diagnostic value for rheumatoid arthritis (RA) of anti-filaggrin autoantibodies (autoAb) recognizing citrullinated recombinant rat filaggrin (ACRF) in community cases of very early arthritis. To evaluate the diagnostic value of ACRF, were studied sera from patients with ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,多中心研究

    doi:10.1111/j.1365-2249.2004.02341.x

    authors: Vittecoq O,Incaurgarat B,Jouen-Beades F,Legoedec J,Letourneur O,Rolland D,Gervasi G,Ménard JF,Gayet A,Fardellone P,Daragon A,Jolivet M,le Loët X,Tron F

    更新日期:2004-01-01 00:00:00

  • Effect of nandrolone decanoate on Sjögren's syndrome like disorders in NZB/NZW mice.

    abstract::The effects of 2.5-40 mg/kg nandrolone decanoate (ND) on the development and growth of mononuclear cell infiltrations in the submandibular glands of NZB/W (B/W) mice have been studied using a quantitative histological method. Injections with ND for 9 months, once every 3 weeks, starting at 4 weeks of age, reduced the ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Schot LP,Verheul HA,Schuurs AH

    更新日期:1984-09-01 00:00:00

  • Natural autoantibodies, IgG antibodies to tetanus toxoid and CD5+ B cells in patients with Mediterranean visceral leishmaniasis. The Leishmania Study Group.

    abstract::Natural autoantibodies (NaAb) and IgG antibodies to tetanus toxoid (TT) were analysed in the sera of 38 children with active visceral leishmaniasis (VL) previously vaccinated with TT and in 30 healthy controls matched for sex and age. Patients exhibited high levels of NaAb to a panel of self antigens (tubulin, myosin,...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.1994.tb07022.x

    authors: Louzir H,Belal-Kacemi L,Sassi A,Laouini D,Ben Ismail R,Dellagi K

    更新日期:1994-03-01 00:00:00

  • Defective antigen presentation by lavage cells from terminal patients with cystic fibrosis.

    abstract::Macrophages separated from lavage samples obtained from lungs removed at transplantation from patients with cystic fibrosis (CF) and other lung diseases, have been compared with circulating monocytes from the same patients for their ability to stimulate allogeneic normal circulating lymphocytes and to present antigen ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2249.1997.d01-954.x

    authors: Knight RA,Kollnberger S,Madden B,Yacoub M,Hodson ME

    更新日期:1997-03-01 00:00:00

  • C3 nephritic factor and hypocomplementaemia in a clinically healthy individual.

    abstract::Nephritic factor was detected in an individual whose serum showed a selective decrease in the third component of complement (C3) during and subsequent to an illness resembling disseminated gonococcal infection (DGI). As is characteristic of C3 nephritic factor (C3NeF), its activity was heat stable, was associated with...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Gewurz AT,Imherr SM,Strauss S,Gewurz H,Mold C

    更新日期:1983-10-01 00:00:00

  • Antibodies from patients with psoriasis recognize N-acetylglucosamine terminals in glycoproteins from Pityrosporum ovale.

    abstract::We have previously reported the finding of circulating antibodies recognizing two proteins of 100 and 120 kD (PO100 and PO120) from Pityrosporum ovale in patients with psoriasis. These antibodies were specific, since they were not detected in normal sera nor in other diseases linked to P. ovale such as seborrhoeic der...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2249.1996.d01-719.x

    authors: Mathov I,Plotkin L,Abatangelo C,Galimberti R,Squiquera L,Leoni J

    更新日期:1996-07-01 00:00:00

  • Two novel cationic staphylococcal proteins induce IL-2 secretion, proliferation and immunoglobulin synthesis in peripheral blood mononuclear cells (PBMC) of both healthy controls and patients with common variable immunodeficiency (CVID).

    abstract::Two cationic proteins, a neutral phosphatase (NP-tase) and a 70-kD protein (p70) were isolated from Staphylococcus aureus by ion exchange chromatography. We compared their properties to those of the well established B cell mitogen of whole, fixed Staph. aureus strain Cowan I cells (SAC). Both purified proteins were ab...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.1995.tb03714.x

    authors: Jahreis A,Yousif Y,Rump JA,Dräger R,Vogt A,Peter HH,Schlesier M

    更新日期:1995-06-01 00:00:00

  • Subsets of T lymphocytes in relation to T lymphocyte function in multiple sclerosis.

    abstract::T lymphocyte control of Epstein-Barr virus (EBV) infection of autologous B lymphocytes was examined in parallel to the enumeration of subpopulations of mononuclear cells in 22 multiple sclerosis (MS) patients and in 22 healthy individuals. All were seropositive for EBV. The incidence of lack of T cell control was sign...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Craig JC,Hawkins SA,Swallow MW,Lyttle JA,Patterson VH,Merrett JD,Haire M

    更新日期:1985-09-01 00:00:00

  • Impaired CD4 and CD8 T cell phenotype and reduced chemokine secretion in recent-onset type 1 diabetic children.

    abstract::Although the role of the T cell-mediated autoimmune reaction in type 1 diabetes (T1D) is conclusive, studies including data from human circulating CD4(+) and CD8(+) lymphocytes subsets during the disease onset and posterior development are scarce. Further, chemokines and chemokine receptors are key players in the migr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,多中心研究

    doi:10.1111/j.1365-2249.2008.03720.x

    authors: Hedman M,Faresjö M,Axelsson S,Ludvigsson J,Casas R

    更新日期:2008-09-01 00:00:00

  • Eosinophil involvement in rheumatoid arthritis as reflected by elevated serum levels of eosinophil cationic protein.

    abstract::Circulating levels of eosinophil cationic protein (ECP), an eosinophil specific granule protein, and numbers of peripheral eosinophils were determined in 42 patients with rheumatoid arthritis. At the time of the investigation the patients were without drug treatment. They had normal blood counts of eosinophils but on ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Hällgren R,Feltelius N,Svenson K,Venge P

    更新日期:1985-03-01 00:00:00

  • Strong prostaglandin associated suppression of the proliferation of human maternal lymphocytes by neonatal lymphocytes linked to T versus T cell interactions and differential PGE2 sensitivity.

    abstract::Lymphocytes from human fetuses and newborns strongly, regularly, and non-specifically suppress the proliferation of PHA stimulated peripheral blood mononuclear leucocytes. The suppression is prostaglandin (PG)-dependent. Our present investigation clearly indicates that the suppression is associated with neonatal T ver...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Papadogiannakis N,Johnsen SA,Olding LB

    更新日期:1985-07-01 00:00:00

  • Circulating immune complexes in six patients with trichinosis.

    abstract::The sera of six patients infected with Trichinella spiralis were studied for the presence of circulating immune complexes (CIC). CIC were present in all six patients studied 1 month after infection. In two patients in whom serial serum samples were available, as clinical improvement occurred there was a decrease in th...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Géniteau M,Verroust PJ,Smith MD,Morel-Maroger L,Saimot G,Coulaud JP

    更新日期:1977-10-01 00:00:00

  • Changes in the phenotype of monocytes/macrophages and expression of cytokine mRNA in peripheral blood and synovial fluid of patients with rheumatoid arthritis.

    abstract::Data from a previous study suggested that peripheral blood monocytes in patients with rheumatoid arthritis (RA) may be activated. Therefore, in this study we sought further evidence of 'presynovial' activation of monocytes. Our results show that phenotypic changes are demonstrable in peripheral blood monocytes in pati...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.1995.tb03850.x

    authors: Highton J,Carlisle B,Palmer DG

    更新日期:1995-12-01 00:00:00

  • In vitro histamine and serotonin release by radiographic contrast media (RCM). Complement-dependent and -independent release reaction and changes in ultrastructure of human blood cells.

    abstract::Peripheral leucocytes and platelets from twenty healthy volunteers were incubated in vitro with radiographic contrast media (diatrizoate-Hypaque, iothalamate-Conray and iodipamide-Cholografin) under varying conditions. All radiographic contrast media (RCM) were able to induce histamine release from peripheral leucocyt...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Ring J,Arroyave CM,Frizler MJ,Tan EM

    更新日期:1978-04-01 00:00:00

  • Impaired interleukin (IL)-4-associated generation of CCR4-expressing T cells in neonates with hereditary allergy risk.

    abstract::Reduced microbial exposure in early life may contribute to the increase of atopic diseases in 'westernized' societies but the underlying mechanisms remain elusive. The objective of this study was to examine how exposure to bacterial lipopolysaccharide (LPS) during early antigen encounter might influence the maturation...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.2005.02706.x

    authors: Haddeland U,Sletten GB,Brandtzaeg P,Nakstad B

    更新日期:2005-02-01 00:00:00

  • Immunoglobulin (Ig)M antibodies against oxidized cardiolipin but not native cardiolipin are novel biomarkers in haemodialysis patients, associated negatively with mortality.

    abstract::The risk of premature death is high in haemodialysis (HD) patients. Antibodies against cardiolipin (anti-CL) are thrombogenic in diseases such as systemic lupus erythematosus (SLE). CL is easily oxidized (Ox) and plays a role in apoptosis. In this work we studied immunoglobulin (Ig)M anti-CL and anti-OxCL in HD-patien...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/cei.12181

    authors: Frostegård AG,Hua X,Su J,Carrero JJ,Heimbürger O,Bárány P,Stenvinkel P,Frostegård J

    更新日期:2013-12-01 00:00:00

  • Immune status in Crohn's disease. V. Decreased in vitro natural killer cell activity in peripheral blood.

    abstract::To identify and measure the spontaneous cell-mediated cytotoxicity of natural killer cells, we used the lysis of an established lymphoblastoid cell line as target in a 4-hr 51Cr-release assay. Mononuclear cell suspensions of the peripheral blood of thirty-four patients with Crohn's disease (group CD), eleven patients ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Auer IO,Ziemer E,Sommer H

    更新日期:1980-10-01 00:00:00

  • Study of cell changes induced by radiolabelling of human lymphocytes with 99m-technetium.

    abstract::With the aim of evaluating cell changes possibly induced by labelling lymphocytes with 99m-technetium (99mTc), several rosette tests and stimulation assays with mitogens have been carried out before and after radiolabelling. The results presented here show that (i) as the rosette tests are not modified statistically, ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Doly A,Finat-Duclos F,Doly M,Veyre A,Plagne R,Meyniel G

    更新日期:1983-06-01 00:00:00

  • Serosuppression in experimental filariasis.

    abstract::Both antigen specific and non-specific immunoregulation by cells have been described in jirds infected with Brugia pahangi, but the contribution of serum factors to immunoregulatory phenomena in this infection has not been examined. The present study determined the effect of serum from normal or B. pahangi infected ji...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Lammie PJ,Katz SP,Anderson WH

    更新日期:1984-03-01 00:00:00

  • Seroreactivity with the Plasmodium falciparum blood stage antigen Pf332 in adults and children from malaria-endemic regions.

    abstract::It has earlier been reported that the human monoclonal antibody (MoAb 33G2) and polyclonal antibodies reactive with Pf332 may interfere in vitro with the erythrocytic cycle of Plasmodium falciparum at two potential target sites for protective antibodies, indicating that the antigen may constitute an important target f...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.1993.tb05979.x

    authors: Iqbal J,Perlmann P,Greenwood BM,Berzins K

    更新日期:1993-10-01 00:00:00

  • Natural killer cell activities of synbiotic Lactobacillus casei ssp. casei in conjunction with dextran.

    abstract::We have reported previously that Lactobacillus casei ssp. casei, together with specific substrate dextran, exhibited an adjuvant effect of stimulating humoral immune responses against bovine serum albumin (BSA) as a model antigen in BALB/c mice. In the present study, among the Lactobacillus species tested, L. casei ss...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.2005.02975.x

    authors: Ogawa T,Asai Y,Tamai R,Makimura Y,Sakamoto H,Hashikawa S,Yasuda K

    更新日期:2006-01-01 00:00:00