Direct Coombs antiglobulin reactions in Gambian children with Plasmodium falciparum malaria. I. Incidence and class specificity.

abstract：:The technical details of a fixation procedure with formaldehyde which was applied in a direct membrane immunofluorescence technique to mononuclear cells from normal human blood are described. After separation of the cells with Ficoll--Isopaque according to Böyum (1963) they were washed and fixed with 0 . 04% formaldeh...

journal_title：Clinical and experimental immunology

authors： Schuit HR,Hijmans W,Asma GE

更新日期：1980-09-01 00:00:00

abstract：:Reliable risk assessment for biotherapeutics requires accurate evaluation of risk factors associated with immunogenicity. Immunogenicity risk assessment tools were developed and applied to investigate the immunogenicity of a fully human therapeutic monoclonal antibody, ATR-107 [anti-interleukin (IL)-21 receptor] that ...

journal_title：Clinical and experimental immunology

authors： Xue L,Hickling T,Song R,Nowak J,Rup B

更新日期：2016-01-01 00:00:00

abstract：:Spontaneous sperm agglutination was observed in the ejaculate of a 39-year-old male (patient Z). Using an enzyme-linked immunosorbent assay (ELISA) with spermatozoa fixed to wells of a microtitre plate it was determined that IgA was associated with patient Z's spermatozoa but not with a pooled sample of spermatozoa fr...

journal_title：Clinical and experimental immunology

authors： Witkin SS,Zelikovsky G,Good RA,Day NK

更新日期：1981-05-01 00:00:00

abstract：:We describe the usefulness of a fast (48-h) limiting dilution assay (LDA) for the enumeration of human alloreactive helper T lymphocytes (HTL) in the peripheral blood, in relation to histologically defined rejection grades after heart transplantation. HTL frequencies (HTLf) in pretransplant samples varied from patient...

journal_title：Clinical and experimental immunology

authors： Vaessen LM,Daane CR,Maat AP,Balk AH,Claas FH,Weimar W

更新日期：1999-12-01 00:00:00

abstract：:One approach to study the role of distinct cellular mechanisms in susceptibility/resistance to tuberculosis (TB) is to compare parameters of response to infection in the lungs of mouse strains exhibiting genetically determined differences in TB susceptibility/severity. Interstrain differences in antimycobacterial macr...

journal_title：Clinical and experimental immunology

authors： Eruslanov EB,Majorov KB,Orlova MO,Mischenko VV,Kondratieva TK,Apt AS,Lyadova IV

更新日期：2004-01-01 00:00:00

abstract：:Immune responses towards malignant plasma cells have clearly been demonstrated in the course of monoclonal B cell dyscrasias and shown to be mostly specific for idiotypic determinants of the monoclonal immunoglobulin (Ig). These responses are specifically efficient against lymphoma cells expressing a membrane form of ...

journal_title：Clinical and experimental immunology

authors： Galea HR,Cogné M

更新日期：2002-08-01 00:00:00

abstract：:Calculated globulin (total protein - albumin) is usually tested as part of a liver function test profile in both primary and secondary care and determines the serum globulin concentration, of which immunoglobulins are a major component. The main use hitherto of calculated globulin is to detect paraproteins when the le...

journal_title：Clinical and experimental immunology

authors： Jolles S,Borrell R,Zouwail S,Heaps A,Sharp H,Moody M,Selwood C,Williams P,Phillips C,Hood K,Holding S,El Shanawany T

更新日期：2014-09-01 00:00:00

abstract：:To identify and measure the spontaneous cell-mediated cytotoxicity of natural killer cells, we used the lysis of an established lymphoblastoid cell line as target in a 4-hr 51Cr-release assay. Mononuclear cell suspensions of the peripheral blood of thirty-four patients with Crohn's disease (group CD), eleven patients ...

journal_title：Clinical and experimental immunology

authors： Auer IO,Ziemer E,Sommer H

更新日期：1980-10-01 00:00:00

abstract：:It is well known that monocytes may play an active role in thrombogenesis, since they may express on their surface tissue factor, the major initiator of the clotting cascade. The results of this investigation demonstrate beta-2-glycoprotein I (beta2-GPI) mRNA expression by human peripheral blood monocytes, indicating ...

journal_title：Clinical and experimental immunology

authors： Conti F,Sorice M,Circella A,Alessandri C,Pittoni V,Caronti B,Calderaro C,Griggi T,Misasi R,Valesini G

更新日期：2003-06-01 00:00:00

abstract：:Cellular immunity with interferon gamma production could have a role in protection from hepatitis C virus (HCV). Interleukin (IL)-12 is a key cytokine in promoting such anti-viral T helper 1 (Th1) responses. We hypothesized that a genetic background able to promote cellular responses may be associated with apparent pr...

journal_title：Clinical and experimental immunology

authors： Hegazy D,Thurairajah P,Metzner M,Houldsworth A,Shaw S,Kaminski E,Demaine AG,Cramp ME

更新日期：2008-06-01 00:00:00

abstract：:Lymphocyte transformation test has often been used as an in vitro test for nickel allergy. We have previously demonstrated the presence of nickel-reactive T cells in individuals with no history of allergic disease and with a negative patch test towards NiSO4. In this study, we show that this proliferative response was...

journal_title：Clinical and experimental immunology

authors： Lisby S,Hansen LH,Menn T,Baadsgaard O

更新日期：1999-08-01 00:00:00

abstract：:One hundred and seventy-five sera from thirty-three patients with acute myeloid leukaemia, forty-two patients with chronic myeloid leukaemia and twelve patients with acute lymphatic leukaemia were examined by a radioimmunological technique for the presence of antibodies against single-stranded and double-stranded DNA....

journal_title：Clinical and experimental immunology

authors： Izui S,Lambert PH,Carpentier N,Miescher PA

更新日期：1976-06-01 00:00:00

abstract：:Nucleotide-binding, oligomerization domain (NOD)-like receptor family, pyrin domain containing 3 (NLRP3) gene polymorphism was reported to be associated with susceptibility, disease activity or anti-tumour necrosis factor (TNF) treatment response in rheumatoid arthritis (RA). However, the roles of NLRP3 inflammasome i...

journal_title：Clinical and experimental immunology

authors： Guo C,Fu R,Wang S,Huang Y,Li X,Zhou M,Zhao J,Yang N

更新日期：2018-11-01 00:00:00

abstract：:Activation of human peripheral blood lymphocytes from unsensitized donors by fractions of T.b rhodesiense has been demonstrated in vitro. Both T and B cells were stimulated to differentiate into blast cells. Purified T cells failed to proliferate, but did so when mitomycin C-treated non-T cells were added. Non-T cell ...

journal_title：Clinical and experimental immunology

authors： Selkirk ME,Ogilvie BM,Platts-Mills TA

更新日期：1981-09-01 00:00:00

abstract：:Cells from the peripheral blood of patients with B cell chronic lymphocytic leukaemia (B-CLL) and acute lymphoblastic leukaemia (ALL) were examined for the expression of MHC class II antigens, using a number of monoclonal antibodies (MoAb) including L243 (anti-DR) and TU22 (anti-DQ). There was wide variation in expres...

journal_title：Clinical and experimental immunology

authors： Guy K,Docherty LJ,Dewar AE

更新日期：1986-02-01 00:00:00

abstract：:Environmental factors such as diet are known to play important roles in inflammatory bowel disease (IBD). Epidemiological studies have indicated that a high-fat diet is a risk factor for IBD. In addition, the balance between effector T cells (T(eff)) and regulatory T cells (T(reg)) contributes to the pathogenesis of m...

journal_title：Clinical and experimental immunology

authors： Ma X,Torbenson M,Hamad AR,Soloski MJ,Li Z

更新日期：2008-01-01 00:00:00

abstract：:Appropriate activation of the immune system and effective targeting of tumour cells are the primary hurdles to be overcome for cancer immunotherapy to be successful and applicable to a wide range of tumour types. Our studies have examined the ability of bacterial-stimulated dendritic cells (DCs), loaded with tumour-as...

journal_title：Clinical and experimental immunology

authors： Shilling DA,Smith MJ,Tyther R,Sheehan D,England K,Kavanagh EG,Redmond HP,Shanahan F,O'Mahony L

更新日期：2007-07-01 00:00:00

abstract：:Autoimmune Addison's disease (AAD), or primary adrenocortical insufficiency, is a classical organ-specific autoimmune disease with 160 years of history. AAD is remarkably homogeneous with one major dominant self-antigen, the cytochrome P450 21-hydroxylase enzyme, which is targeted by both autoantibodies and autoreacti...

journal_title：Clinical and experimental immunology

authors： Hellesen A,Bratland E

更新日期：2019-01-01 00:00:00

abstract：:Anti-endothelial cell antibodies (AECA) have been frequently detected in systemic vasculitis, which affects blood vessels of various sizes. To understand the pathogenic roles of AECA in systemic vasculitis, we attempted to identify target antigens for AECA comprehensively by a proteomic approach. Proteins extracted fr...

journal_title：Clinical and experimental immunology

authors： Karasawa R,Kurokawa MS,Yudoh K,Masuko K,Ozaki S,Kato T

更新日期：2010-09-01 00:00:00

abstract：:We previously demonstrated that high levels of IL-6/sIL-6R complexes are present in sera of patients with systemic juvenile idiopathic arthritis (s-JIA) and that the amount of IL-6 estimated in the IL-6/sIL-6R complexes is markedly higher than that measured by the B9 assay. Here, we show that two additional bioassays,...

journal_title：Clinical and experimental immunology

authors： Pignatti P,Ciapponi L,Galle P,Hansen MB,Massa M,Meazza C,Paonessa G,Novick D,Ciliberto G,Martini A,De Benedetti F

更新日期：2003-02-01 00:00:00

abstract：:Atopic disorders are caused by disregulated activation of T helper 2 (Th2) cells that produce IL-4 and IL-5. Because the presence of IL-4 potently augments the differentiation of naive T cells into Th2 cells, it is important to seek the cell population which provides IL-4 for naive T cells. Recently, a unique subpopul...

journal_title：Clinical and experimental immunology

authors： Oishi Y,Sakamoto A,Kurasawa K,Nakajima H,Nakao A,Nakagawa N,Tanabe E,Saito Y,Iwamoto I

更新日期：2000-03-01 00:00:00

abstract：:Hypersensitivity to Chironomidae (non-biting midges) has been a problem in Northern Sudan since about 1927 and is probably due to the working of dams which have produced lake-like conditions on parts of the Blue and main Niles where breeding has evidently increased. Studies were undertaken to determine whether this hy...

journal_title：Clinical and experimental immunology

authors： Kay AB,Gad El Rab MO,Stewart J,Erwa HH

更新日期：1978-10-01 00:00:00

abstract：:Eosinophils contribute to the pathogenesis of bullous pemphigoid (BP) by secretion of proinflammatory cytokines and proteases. Trafficking of eosinophils into tissue in animal models and asthma depends on interleukin-5 and a family of chemokines named eotaxins, comprising CCL11, CCL24 and CCL26. Up-regulation of CCL11...

journal_title：Clinical and experimental immunology

authors： Günther C,Wozel G,Meurer M,Pfeiffer C

更新日期：2011-11-01 00:00:00

abstract：:Conversion of arginyl to citrullyl residues (citrullination) is essential for the formation of the epitopes recognized by rheumatoid arthritis (RA)-associated autoantibodies to citrullinated proteins (ACPA). ACPA are secreted by plasma cells of the rheumatoid synovial tissue where their major target, citrullinated fib...

journal_title：Clinical and experimental immunology

authors： Duplan V,Foulquier C,Clavel C,Al Badine R,Serre G,Saoudi A,Sebbag M

更新日期：2006-09-01 00:00:00

abstract：:Secretory IgA antibody responses such as those directed against respiratory and enteric pathogens, may be impaired in protein-calorie malnutrition. In previous studies using a rat model of the mucosal immune response to cholera toxin, as little as 2 weeks of severe protein deprivation markedly impaired mucosal anti-to...

journal_title：Clinical and experimental immunology

authors： Koster F,Pierce NF

更新日期：1985-04-01 00:00:00

abstract：:In the present study we examined the role of thymic stromal lymphopoietin (TSLP) in experimental autoimmune encephalomyelitis (EAE). Here, we report that TSLP knock-out (KO) mice display a delayed onset of disease and an attenuated form of EAE. This delayed onset was accompanied by a reduced number of encephalitogenic...

journal_title：Clinical and experimental immunology

authors： Eckhardt J,Döbbeler M,König C,Kuczera K,Kuhnt C,Ostalecki C,Zinser E,Mak TW,Steinkasserer A,Lechmann M

更新日期：2015-07-01 00:00:00

abstract：:Monoclonal anti-dermatan sulphate antibodies were produced with hybridization after immunizing BALB/c mice with dermatan sulphate (DS). These antibodies were tested for cross-reactivity to double-stranded DNA (dsDNA) and heat-denatured DNA (ssDNA). Five clones had binding activity to DS, dsDNA and ssDNA in enzyme-link...

journal_title：Clinical and experimental immunology

authors： Ohnishi K,Sagawa A,Nakabayashi T,Ohmoto A,Megumi,Yoshikawa M,Watanabe I,Mukai M,Yasuda I,Nakagawa S

更新日期：1989-01-01 00:00:00

abstract：:This study evaluates the correlation between natural killer (NK) cell function and human immunodeficiency virus (HIV)-1 disease progression in 133 untreated HIV-1 positive Chinese subjects, including 41 former plasma donors (FPDs) and 92 men who have sex with men, and 35 HIV-negative controls. Flow cytometry was used ...

journal_title：Clinical and experimental immunology

authors： Jia M,Li D,He X,Zhao Y,Peng H,Ma P,Hong K,Liang H,Shao Y

更新日期：2013-01-01 00:00:00

abstract：:Human cartilage gp-39 (HC gp-39) is a well-known autoantigen in rheumatoid arthritis (RA). However, the exact localization, fluctuation and function of HC gp-39 in RA are unknown. Therefore, using a glucose-6-phosphate isomerase (GPI)-induced model of arthritis, we investigated these aspects of HC gp-39 in arthritis. ...

journal_title：Clinical and experimental immunology

authors： Tanaka Y,Matsumoto I,Inoue A,Umeda N,Takai C,Sumida T

更新日期：2014-08-01 00:00:00

abstract：:Biologically significant levels of IL-2 activity were produced by isolated lamina propria mononuclear cells (LPMC) from normal intestine (n = 12), cancer-bearing colons (n = 35) and inflammatory bowel disease (IBD) affected tissue (n = 12). The levels of IL-2 produced were similar for all three sources of LPMC (normal...

journal_title：Clinical and experimental immunology

authors： Pullman WE,Doe WF

更新日期：1992-04-01 00:00:00