Abstract：
:We previously demonstrated that high levels of IL-6/sIL-6R complexes are present in sera of patients with systemic juvenile idiopathic arthritis (s-JIA) and that the amount of IL-6 estimated in the IL-6/sIL-6R complexes is markedly higher than that measured by the B9 assay. Here, we show that two additional bioassays, employing human myeloma XG-1 cells and human hepatoma Hep3B cells, detected serum IL-6 levels similar to those measured by the B9 assay and approximately 10-fold lower than the IL-6 levels estimated to be present in the IL-6/sIL-6R complex. Using an assay for the measurement of the amount of circulating IL-6 complexed with the sIL-6R and available for binding to gp130 (gp130 binding activity), we show that the IL-6/gp130 binding activity is similar to that detected by the bioassays and again significantly lower than that estimated to be present in the IL-6/sIL-6R complex. Addition of recombinant human IL-6 (rhIL-6) to sera of patients or controls results in a markedly lower increase in the gp130 binding activity in patients than in controls. Moreover, sera from s-JIA patients inhibited in a dose dependent manner the gp130 binding activity assay. These results show that sera from patients with s-JIA contain a factor, or factors, that inhibit(s) the binding of the IL-6/sIL-6R complex to gp130. This inhibitory activity does not appear to be due to soluble gp130, C-reactive protein or autoantibodies to IL-6.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Pignatti P,Ciapponi L,Galle P,Hansen MB,Massa M,Meazza C,Paonessa G,Novick D,Ciliberto G,Martini A,De Benedetti Fdoi
10.1046/j.1365-2249.2003.02052.xsubject
Has Abstractpub_date
2003-02-01 00:00:00pages
355-63issue
2eissn
0009-9104issn
1365-2249pii
2052journal_volume
131pub_type
杂志文章abstract：:The capacity of B cells from patients with chronic lymphocytic leukaemia (CLL) to produce and secrete immunoglobulin following mitogen stimulation was investigated using sensitive radioimmunoassays for mu, gamma, alpha, kappa, and lambda immunoglobulin chains. Lymphocytes from seven of the 11 patients studied secreted...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-03-01 00:00:00
abstract：:Thirty sera reacting by IFL technique in titres greater than or equal to 100 with smooth muscle fibres of rat stomach, rat renal glomeruli, and with the membrane region of thyroid cells were randomly chosen among sera sent in for routine testing of tissue antibodies. All sera but one were found to be derived from pati...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
abstract：:Two-stage stimulation of SK activity of normal human peripheral blood lymphocytes by soluble factors was demonstrated. The first stage was initiated by factors present in supernatant derived from normal B-LCL cultures. Only cell lines which could induce SK activity in culture in an MLR-type reaction had the capacity t...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-12-01 00:00:00
abstract：:Two kinds of brush border antigens were isolated from pronase-treated rat tubular material by gel filtration, DEAE-chromatography and disc-electrophoresis, successively. One, a 0.05 M antigen which was eluted from DEAE-column with 0.05 mol of NaCl solution, has no nephritogenic ability when inoculated into homologous ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-12-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-11-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2011.04324.x
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journal_title：Clinical and experimental immunology
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1982-05-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 临床试验,杂志文章
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更新日期：1998-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：2004-04-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1978-07-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-12-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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