Abstract：
:Spontaneous sperm agglutination was observed in the ejaculate of a 39-year-old male (patient Z). Using an enzyme-linked immunosorbent assay (ELISA) with spermatozoa fixed to wells of a microtitre plate it was determined that IgA was associated with patient Z's spermatozoa but not with a pooled sample of spermatozoa from fertile men Z's seminal fluid agglutinated human spermatozoa (titre 1:16). In contrast, no sperm-agglutinating or immobilizing activity was present in the patient's serum. Agglutination was prevented by preincubation of the seminal fluid with anti-IgA antibody but not with anti-IgA antibody. IgG from Z's seminal fluid readily bound to human spermatozoa, as determined by the ELISA assay. IgA from a pool of seminal fluids from fertile men or from 18 human sera bound to spermatozoa to a much lesser extent. By sucrose-gradient velocity centrifugation it was demonstrated that the anti-sperm IgA in patient Z's seminal fluid was 11S. Finally, antibody to secretory component removed the anti-sperm IgA from Z's seminal fluid. These data provide evidence that secretory IgA with anti-sperm activity is produced in the male genital tract. Furthermore, a new methodology is presented for the determination of antibodies to spermatozoa.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Witkin SS,Zelikovsky G,Good RA,Day NKsubject
Has Abstractpub_date
1981-05-01 00:00:00pages
368-74issue
2eissn
0009-9104issn
1365-2249journal_volume
44pub_type
杂志文章abstract：:Immunoenzymatic assays were developed for the measurement of antibodies against mycobacterial lipoarabinomannan (LAM), a cell-free proteic extract (CFX) of Mycobacterium leprae, and the 38-kD protein antigen of M. tuberculosis. Sera from 108 leprosy patients, belonging to all clinical-immunological forms of the spectr...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1998.00452.x
更新日期：1998-01-01 00:00:00
abstract：:Previous studies using isolated complement proteins have shown that more C4A than C4B binds to certain types of immune complexes. However, the in vivo binding of the C4 isoforms to an immune complex has not been investigated in detail and may differ from events when measured with the isolated proteins. We report here ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1999.00940.x
更新日期：1999-07-01 00:00:00
abstract：:The serum levels of TGF-beta1, measured by solid-phase ELISA, were determined to be significantly augmented in patients with both relapsing remitting (RR) and secondary chronic progressive (CP) MS compared with sex- and age-matched healthy controls. Moreover, in RR MS patients, the blood levels of the cytokine were fu...
journal_title：Clinical and experimental immunology
pub_type： 临床试验,杂志文章
doi：10.1046/j.1365-2249.1998.00604.x
更新日期：1998-07-01 00:00:00
abstract：:The emerging role of interleukin (IL)-17 as a hallmark proinflammatory cytokine of the adaptive immune system, produced primarily by a new T helper cell subset termed 'Th17', has received considerable attention. Differentiation of Th17 cells is driven by the simultaneous presence of transforming growth factor-beta and...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2249.2009.03944.x
更新日期：2009-08-01 00:00:00
abstract：:We have compared features of experimental thyroiditis in the Buffalo strain rat induced by neonatal thymectomy, immunization with rat thyroglobulin (Tg) and complete Freund's adjuvant, and subcutaneous administration of trypan blue or 3-methylcholanthrene. The disease produced by neonatal thymectomy resulted in signif...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-07-01 00:00:00
abstract：:Hepatitis B (HB) vaccine is very promising for the prevention of HB infection. There exist, however, some non-responders to current vaccination trials. In this study, taurine, parotin and lithium were selected as adjuvants which can be administered orally. The mechanisms of these three materials as adjuvants and their...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:The introduction of 25 mg of a new protein antigen into the diets of mice produced transient increases in PVP clearance which reached a maximum 60 hr after the change of diet and had disappeared by 4 days. At the same time the immune responsiveness to unrelated antigens given parenterally was also raised transiently, ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-02-01 00:00:00
abstract：:Leucocyte adhesion deficiency type 1 (LAD-1) is characterized by the incapacity of leucocytes to carry out their adhesion functions via their CD11/CD18 antigens, which are also referred to as the leucocyte integrins. The patients generally suffer from poor wound healing and recurrent bacterial and fungal infections. I...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2000.01277.x
更新日期：2000-07-01 00:00:00
abstract：:This longitudinal study investigates the change of erythrocyte complement receptor (E-CR1) expression in patients with severe acute respiratory syndrome (SARS). Circulating E-CR1 expression was semiquantified by flow cytometric analyses in 54 SARS patients and in 212 healthy individuals as a control. Since E-CR1 expre...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2005.02681.x
更新日期：2005-01-01 00:00:00
abstract：:The effect of various exogenous cells on the in-vitro PWM stimulation of immunoglobulin synthesis by normal human peripheral blood mononuclear cells was investigated. The accessory cells tested were bovine and human endothelial cells, bovine and human fibroblasts, normal human monocytes, and a monocyte cell line U937....
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-09-01 00:00:00
abstract：:Vacuum-assisted closure involving the implantation of polyvinyl alcohol foam is a technique recently developed for the treatment of patients suffering from either wound infection or chronic wounds. This method has been shown to improve and accelerate wound healing. However, little is known about the cell populations t...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2001.01547.x
更新日期：2001-06-01 00:00:00
abstract：:The effect of a standard laparatomy on antibody synthesis was studied in Wistar R/A rats recieving an intravenous injection of 10(9) sheep red blood cells (SRBC) during the surgical procedure. Anti-SRBC antibody titres were significantly higher in operated animals than in controls. When SRBC were given 2 hr after the ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1978-05-01 00:00:00
abstract：:A growing body of evidence points to autophagy as an essential component in the immune response to tuberculosis. Autophagy is a direct mechanism of killing intracellular Mycobacterium tuberculosis and also acts as a modulator of proinflammatory cytokine secretion. In addition, autophagy plays a key role in antigen pro...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2249.2011.04381.x
更新日期：2011-06-01 00:00:00
abstract：:Patients with common variable immunodeficiency disorders are monitored for liver function test abnormalities. A proportion of patients develop deranged liver function and some also develop hepatomegaly. We investigated the prevalence of abnormalities and types of liver disease, aiming to identify those at risk and det...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2008.03711.x
更新日期：2008-09-01 00:00:00
abstract：:Human bronchoalveolar lavage (BAL) macrophages were obtained from normal human volunteers and infected with an AIDS-associated strain of Mycobacterium avium. Infected cells were exposed to purified envelope glycoprotein (gp120) from HIV-1 or to the recombinant non-glycosylated gp120 fragments PBI-RF and PBI-IIIB. Nati...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1994.tb06617.x
更新日期：1994-10-01 00:00:00
abstract：:The capacity of B cells from patients with chronic lymphocytic leukaemia (CLL) to produce and secrete immunoglobulin following mitogen stimulation was investigated using sensitive radioimmunoassays for mu, gamma, alpha, kappa, and lambda immunoglobulin chains. Lymphocytes from seven of the 11 patients studied secreted...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-03-01 00:00:00
abstract：:Glomerulonephritis with organized microtubular monoclonal immunoglobulin deposits (GOMMID) and glomerulonephritis related to type I cryoglobulin are well-known but rare complications of B cell derived chronic lymphocytic leukaemia. In these disorders, monoclonal Ig have never been studied at the molecular level. We co...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2002.01896.x
更新日期：2002-07-01 00:00:00
abstract：:Five out of 51 sera (10%) from patients with progressive systemic sclerosis reacted by immunoblotting with tissue concanavalin A binding glycoproteins of 50 kD and 45 kD Mr, whereas only one out of 133 control sera gave the same reaction (P less than 0.001). The antigens were localized to the microsomal fractions of t...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:Studies were performed on eleven patients with inflammatory bowel disease to determine if there was an alteration in concanavalin A (Con A) induced suppressor cell activity. Similar investigations were also performed on twenty-one control subjects and five patients with other inflammatory conditions. Supressor cells w...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1978-06-01 00:00:00
abstract：:It has been postulated that IgA nephropathy (IgAGN) is caused by deposition within the glomerular mesangium of IgA polymers and IgA containing immune complexes which are overproduced in response to antigens presented at mucosal surfaces. To test this, the concentrations of specific antibodies to capsular polysaccharid...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Antibodies to the major phenolic glycolipid purified from Mycobacterium leprae have been demonstrated previously in sera of leprosy but not tuberculosis patients using an ELISA. The major antigenic determinants on this molecule were investigated using antisera raised in rabbits to the purified glycolipid and with a po...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-04-01 00:00:00
abstract：:The incidence of circulating T lymphocytes (ET-RFC) and a sub-population of T lymphocytes (EE-RFC) were monitored in the blood of seventy-one cadaver renal allograft recipients for the first 2 months after transplantation. In patients with uneventful post-operative courses, the incidence of both ET-RFC and EE-RFC fell...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a group of autoimmune diseases, including granulomatosis with polyangiitis (GPA) and microscopic polyangiitis (MPA). It is not known why ANCA develop, but it has been shown that they participate in pathogenesis by activating polymorphonuclear n...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2012.04633.x
更新日期：2012-10-01 00:00:00
abstract：:A method is described which can be used to quantitate class II MHC antigens (HLA-DR) expressed by cells within tissue sections. A mouse anti-human HLA-DR monoclonal antibody is directly conjugated to the fungal enzyme glucose oxidase. The enzyme, in the presence of its substrate can be used to reduce tetrazolium salts...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:Most patients with late-onset bypogammaglobulinaemia have normal numbers of B cells. In addition, some of these patients have been shown to have circulating T cells which suppress immunolobulin production in vitro. From our present studies on a large series of patients it appears that these two findings distinguish tw...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-05-01 00:00:00
abstract：:Groups of subjects during acute (0-3 days) and convalescent (2-3 weeks) phase of recurrent herpes labialis (RHL), and other subjects seropositive or seronegative for herpes simplex virus type 1 (HSV-1) antibody without any history of RHL, were tested for the appearance of cell-mediated cytotoxic responses by stimulati...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:A series of polyclonal and monoclonal anti-human IgE sera reacted with a panel of 11 selected IgA and IgM producing B lymphoblastoid cell lines (BLCLs) in cytoplasmic fluorescence, ELISA and immunoprecipitation assays. The reactions were discordant between the BLCLs and the individual antisera. The panel of 11 BLCLs w...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:The mechanism of action of methotrexate (MTX) in autoimmune diseases (AID) is unclear. A pro-apoptotic effect has been demonstrated in mitogen-stimulated peripheral blood mononuclear cells (PBMC), but studies employing conventional antigens have disputed a pro-apoptotic effect. CD4+ T helper (Th) cells play a signific...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2007.03335.x
更新日期：2007-05-01 00:00:00
abstract：:Environmental factors such as diet are known to play important roles in inflammatory bowel disease (IBD). Epidemiological studies have indicated that a high-fat diet is a risk factor for IBD. In addition, the balance between effector T cells (T(eff)) and regulatory T cells (T(reg)) contributes to the pathogenesis of m...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2007.03530.x
更新日期：2008-01-01 00:00:00
abstract：:In this study, we aimed to explore the effects of imatinib on the proliferation of rheumatoid arthritis synovial cell (RA-FLS) and inflammatory responses by regulating CSF1R. Differential genes were screened via microarray analysis, followed by being analysed through the weighted co-expression network (WGCNA) network,...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13220
更新日期：2019-02-01 00:00:00
